BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,943,931	A→G	noncoding (205/1542 nt)	CXP41_RS20515 →	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,943,931	0	A	G	99.2%	875.5 / ‑1.3	385	noncoding (205/1542 nt)	CXP41_RS20515	16S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base A (3/0); new base G (173/209); total (176/209)
Fisher's exact test for biased strand distribution p-value = 9.46e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 391 total aligned reads displayed.

TAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTC  >  NZ_CP025268/3943796‑3944067

  aCAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGCGGGGGGCCTTCg                                                                                                                                    <  2:2845156/140‑1 (MQ=17)

  aCAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCg                                                                                                                                    <  2:1125182/140‑1 (MQ=17)

     ggAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGc                                                                                                                                 <  2:1374189/140‑1 (MQ=17)

        aaCCGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCctc                                                                                                                              <  1:393513/140‑1 (MQ=17)

        aaCAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCctc                                                                                                                              <  2:6281902/140‑1 (MQ=17)

          cAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCtt                                                                                                                            <  1:4617595/140‑1 (MQ=17)

          cAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCtt                                                                                                                            <  2:2318846/140‑1 (MQ=17)

          cAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGGGAGTAATGTCTGGGAACCTGCCTGATGGAGGGGGATAACTACGGGAAACGGTAGCTAATACCGCATAACGTCGCACGACCAAAGAGGGGGGCCTTCGGGCCTCtt                                                                                                                            >  2:5584648/1‑140 (MQ=11)

              ttGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCa                                                                                                                        <  1:3819228/140‑1 (MQ=17)

                  tGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAg                                                                                                                    <  2:5167495/140‑1 (MQ=18)

                    tttCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGAt                                                                                                                  <  2:1475008/140‑1 (MQ=21)

                    tttCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGAt                                                                                                                  <  2:4180688/140‑1 (MQ=21)

                       cGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGAtgtg                                                                                                               <  2:875765/140‑1 (MQ=18)

                         cTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGcc                                                                                                             <  2:3147357/140‑1 (MQ=17)

                         cTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGcc                                                                                                             <  1:4048529/140‑1 (MQ=17)

                            ccGAGGGGCGGACTGGTGAGTAATGTCTGGGCACCTGCCTGCTGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGAACGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAg                                                                                                          <  2:681221/139‑1 (MQ=11)

                             cGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGa                                                                                                         <  1:6306500/140‑1 (MQ=17)

                             cGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGa                                                                                                         <  1:2725773/140‑1 (MQ=17)

                              gAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGAt                                                                                                        >  1:698198/1‑140 (MQ=17)

                                  ggCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGa                                                                                                    <  1:1641739/140‑1 (MQ=17)

                                   gCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGAt                                                                                                   <  1:1825128/140‑1 (MQ=17)

                                    cGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGCGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGAtt                                                                                                  <  2:2940199/140‑1 (MQ=17)

                                          ggTGATTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCtag                                                                                            <  1:111088/140‑1 (MQ=17)

                                           gTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCtagt                                                                                           <  1:3604311/140‑1 (MQ=17)

                                              aGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAgg                                                                                        <  1:5748321/140‑1 (MQ=17)

                                              aGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAgg                                                                                        >  1:644826/1‑140 (MQ=17)

                                              aGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAgg                                                                                        >  1:1986011/1‑140 (MQ=17)

                                                    gTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGt                                                                                  <  1:2063372/140‑1 (MQ=17)

                                                    gTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGt                                                                                  <  1:2519592/140‑1 (MQ=17)

                                                    gTCTGGGAAACTGCCTGATGGAGGGGGAGAACTACTGGAATCGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGt                                                                                  <  1:4534107/140‑1 (MQ=12)

                                                     tCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTa                                                                                 >  2:1420228/1‑140 (MQ=17)

                                                     tCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTa                                                                                 >  1:1101899/1‑140 (MQ=17)

                                                     tCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCGTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGAGTAGCTAGTAGGTGGGGTa                                                                                 >  1:3723067/1‑140 (MQ=12)

                                                     tCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTa                                                                                 >  2:3718361/1‑140 (MQ=32)

                                                        gggAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACg                                                                              >  2:6360009/1‑140 (MQ=17)

                                                        gggAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACg                                                                              >  2:1252648/1‑140 (MQ=17)

                                                        gggAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACg                                                                              >  1:5874266/1‑140 (MQ=17)

                                                          gAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGc                                                                            >  2:1571424/1‑140 (MQ=17)

                                                          gAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGAGTAGCTAGTAGGTGGGGTAACGGc                                                                            >  2:2587693/1‑140 (MQ=17)

                                                          gAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGGGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGc                                                                            <  1:557838/140‑1 (MQ=17)

                                                           aaaCTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCt                                                                           <  1:5189535/140‑1 (MQ=17)

                                                           aaaCTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGGAGGTGGGGTAACGGCt                                                                           <  2:5269129/140‑1 (MQ=17)

                                                            aaCTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTc                                                                          <  1:1693039/140‑1 (MQ=17)

                                                              cTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCAc                                                                        >  2:3007488/1‑140 (MQ=17)

                                                              cTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCAc                                                                        >  1:1012988/1‑140 (MQ=17)

                                                               tGCCTGATGGAGGGGGATAACTACTGGAACCGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCAcc                                                                       >  2:1341688/1‑140 (MQ=17)

                                                                 ccTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTa                                                                     >  2:3365047/1‑140 (MQ=17)

                                                                   tGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAgg                                                                   <  2:3065529/140‑1 (MQ=17)

                                                                      tGGAGGGGGATAACTACTGGAAACGGNAGCTAATNCCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGNGTAACGGCTCACCTAGGcga                                                                <  1:5637155/140‑1 (MQ=17)

                                                                       tgAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGGCGCAAGACCAAAGACGGGGGACTTCGGGCCTCTTGCCATCAGATGTGCCCAGATTGGATTAGCTATTAGGTGGGGTAACGGCTCACCTAGGcgac                                                               <  2:1890634/139‑1 (MQ=11)

                                                                           ggggATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGCTTAGCTAGTAGGTGGGGTACCGGCTCACCTAGGCGACGATc                                                           >  2:4951317/1‑140 (MQ=12)

                                                                           ggggATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATc                                                           >  2:4291334/1‑140 (MQ=17)

                                                                               aTAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTa                                                       >  2:464717/1‑140 (MQ=17)

                                                                               aTAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTa                                                       >  2:5655977/1‑140 (MQ=17)

                                                                                tAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAg                                                      >  1:3165174/1‑140 (MQ=17)

                                                                                tAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAg                                                      <  1:2423048/140‑1 (MQ=17)

                                                                                tAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGATAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAg                                                      <  2:2719019/140‑1 (MQ=17)

                                                                                 aaCTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGc                                                     <  2:6143142/140‑1 (MQ=17)

                                                                                     actGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGt                                                 <  2:3903733/140‑1 (MQ=17)

                                                                                       tGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCt                                               >  1:2211676/1‑140 (MQ=17)

                                                                                            aCGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGagag                                          <  2:644826/140‑1 (MQ=17)

                                                                                             cGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAgg                                         >  2:2067579/1‑140 (MQ=17)

                                                                                              ggTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGa                                        >  1:4970510/1‑140 (MQ=17)

                                                                                              ggTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGa                                        <  2:3253466/140‑1 (MQ=17)

                                                                                              ggTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGa                                        >  2:3924308/1‑140 (MQ=17)

                                                                                              ggTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGa                                        <  2:4114812/140‑1 (MQ=17)

                                                                                                tAGCTAATACCGCATAACGTCGCAAGACCACAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATg                                      <  2:1759238/140‑1 (MQ=17)

                                                                                                tAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATg                                      >  1:1244366/1‑140 (MQ=17)

                                                                                                   cTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGAcc                                   <  1:3905608/140‑1 (MQ=17)

                                                                                                   cTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGAcc                                   <  1:836255/140‑1 (MQ=17)

                                                                                                   cTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGAGTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGAcc                                   >  2:3581204/1‑140 (MQ=17)

                                                                                                      aTACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCGTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGc                                <  2:4034227/140‑1 (MQ=17)

                                                                                                        aCCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCca                              >  1:1329281/1‑140 (MQ=17)

                                                                                                        aCCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCca                              >  1:3482661/1‑140 (MQ=17)

                                                                                                        aCCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCca                              >  1:4296007/1‑140 (MQ=17)

                                                                                                          cGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCcaca                            >  2:5881941/1‑140 (MQ=17)

                                                                                                            cATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGcgac                                                               <  1:2267367/103‑1 (MQ=17)

                                                                                                            cATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACt                          <  1:4725955/140‑1 (MQ=17)

                                                                                                            cATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACt                          >  1:970307/1‑140 (MQ=17)

                                                                                                            cATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACt                          >  2:4490378/1‑140 (MQ=17)

                                                                                                             aTAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTg                         <  1:3295583/140‑1 (MQ=17)

                                                                                                             aTAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTg                         >  1:5328446/1‑140 (MQ=17)

                                                                                                             aTAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGAAGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTg                         >  2:470510/1‑140 (MQ=17)

                                                                                                                 cGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCAAGATGGGATTAGCTAGTAGGGGGGGTACCGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGCGAGGGTGACCGGCCACACTGGAAc                     >  1:5967558/1‑140 (MQ=11)

                                                                                                                    cGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTga                  >  1:525343/1‑140 (MQ=17)

                                                                                                                      cAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTgaga                >  1:2881296/1‑140 (MQ=17)

                                                                                                                      cAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTgaga                >  1:2125356/1‑140 (MQ=17)

                                                                                                                      cAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTgaga                <  1:3539572/140‑1 (MQ=17)

                                                                                                                      cAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTgaga                >  1:6114276/1‑140 (MQ=17)

                                                                                                                        aGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGaca              <  1:6360010/140‑1 (MQ=17)

                                                                                                                        aGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGaca              <  1:504071/140‑1 (MQ=17)

                                                                                                                        aGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCGAGTAGGTGGGGTACCGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGaca              <  2:4358585/140‑1 (MQ=12)

                                                                                                                           ccAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACgg           >  1:4840628/1‑140 (MQ=17)

                                                                                                                             aaaGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTc         <  1:4181433/140‑1 (MQ=17)

                                                                                                                                gagGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAg      <  2:3475616/140‑1 (MQ=17)

                                                                                                                                 agGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGa     <  2:5478206/140‑1 (MQ=17)

                                                                                                                                   ggggGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTCGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACt   >  2:2233504/1‑140 (MQ=17)

                                                                                                                                   ggggGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACt   >  1:3432147/1‑140 (MQ=17)

                                                                                                                                    ggggCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTc  >  1:3054469/1‑140 (MQ=17)

                                                                                                                                    ggggCCTTCGGGCCTCTTGCCATCAGATGTGCACAGATGGGATTAGCGAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTGTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTc  >  1:4228632/1‑140 (MQ=255)

TAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTC  >  NZ_CP025268/3943796‑3944067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`