BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,215,602	T→C	148 (TAA→TAG)	yjaB ←	acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,215,602	0	T	C	99.8%	1418.0 / NA	406	148 (TAA→TAG)	yjaB	acetyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (250/155); minor base A (1/0); total (251/155)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 406 total aligned reads displayed.

CGCCAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGG  >  NZ_CP025268/4215465‑4215735

     gTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGccc                                                                                                                                <  2:1894656/140‑1 (MQ=255)

     gTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGccc                                                                                                                                <  1:808311/140‑1 (MQ=255)

           caTCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACAt                                                                                                                          >  1:3441684/1‑140 (MQ=255)

           caTCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACAt                                                                                                                          >  1:4290048/1‑140 (MQ=255)

           caTCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACAt                                                                                                                          <  1:2821810/140‑1 (MQ=255)

           caTCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACAt                                                                                                                          >  2:5913842/1‑140 (MQ=255)

                aTGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGACATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGcc                                                                                                                     >  1:1934532/1‑140 (MQ=255)

                aTGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGcc                                                                                                                     >  2:2063873/1‑140 (MQ=255)

                aTGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGcc                                                                                                                     >  1:1094066/1‑140 (MQ=255)

                aTGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGcc                                                                                                                     >  2:5109283/1‑140 (MQ=255)

                 tGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCa                                                                                                                    >  1:3267453/1‑140 (MQ=255)

                 tGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCa                                                                                                                    >  2:4790994/1‑140 (MQ=255)

                 tGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCa                                                                                                                    >  1:1682767/1‑140 (MQ=255)

                  gcgcAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAg                                                                                                                   >  1:139592/1‑140 (MQ=255)

                     cAATCCGCGGTACTCAGAGCTAGAGCGGTCATGGCAATGCTTAACCAGAAATAATCGTGTCCCCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGAGGAGAAAAAATCGCCTACGCCCCCACATACGCCAGAta                                                                                                                <  1:685784/140‑2 (MQ=255)

                       aTCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCa                                                                                                              <  1:6446982/140‑1 (MQ=255)

                        tCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCTGATTTTATCGTGGCAGCATTGGTGGGTACTAAACGTGAAGTGCAGCCCACCGGGATGTGTCTATATCGCCGTCGCGCGCACTTACGCCCGATTCAg                                                                                                             >  2:6192783/1‑140 (MQ=255)

                         cAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGc                                                                                                            <  1:2529793/140‑1 (MQ=255)

                          aGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCAACGCCCCCACATACGCCAGATTCAGCa                                                                                                           <  1:6299029/140‑1 (MQ=255)

                           gCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAACTAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCaa                                                                                                          <  1:553747/140‑1 (MQ=255)

                             ggTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACg                                                                                                        >  1:1540921/1‑140 (MQ=255)

                             ggTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATAGCCTACGCCCCCACATACGCCAGATTCAGCAACg                                                                                                        >  1:944053/1‑140 (MQ=255)

                               tACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGACAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGa                                                                                                      >  2:869965/1‑140 (MQ=255)

                                aCTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGAt                                                                                                     <  1:4790994/140‑1 (MQ=255)

                                aCTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGAt                                                                                                     <  1:1612837/140‑1 (MQ=255)

                                  tCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATag                                                                                                   >  2:2340604/1‑139 (MQ=255)

                                    aGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACgg                                                                                                 >  1:5428145/1‑140 (MQ=255)

                                      tGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAACCCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGtt                                                                                               <  2:6296504/140‑1 (MQ=255)

                                         tAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTcc                                                                                            >  1:3077589/1‑140 (MQ=255)

                                         tAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTcc                                                                                            >  2:3917605/1‑140 (MQ=255)

                                         tAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTcc                                                                                            >  2:700791/1‑140 (MQ=255)

                                         tAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTcc                                                                                            >  2:57297/1‑140 (MQ=255)

                                         tAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTcc                                                                                            >  2:4642446/1‑140 (MQ=255)

                                           gTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACCTACGCCAGATTCAGCAACGGCTACGGTTTcccc                                                                                          >  2:2985400/1‑140 (MQ=255)

                                           gTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTcccc                                                                                          >  1:3031718/1‑140 (MQ=255)

                                           gTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTcccc                                                                                          >  1:77813/1‑140 (MQ=255)

                                            tGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCa                                                                                         >  1:5314596/1‑140 (MQ=255)

                                            tGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCa                                                                                         >  1:2640838/1‑140 (MQ=255)

                                            tGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCa                                                                                         >  2:523111/1‑140 (MQ=255)

                                            tGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCa                                                                                         >  1:3125471/1‑140 (MQ=255)

                                              cGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCaaa                                                                                       <  1:1279725/140‑1 (MQ=255)

                                              cGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCaaa                                                                                       >  2:2254606/1‑140 (MQ=255)

                                                gTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCGAATc                                                                                     >  2:1402729/1‑140 (MQ=255)

                                                gTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATc                                                                                     >  1:4630023/1‑140 (MQ=255)

                                                gTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATc                                                                                     >  2:303474/1‑140 (MQ=255)

                                                gTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATc                                                                                     >  2:2433490/1‑140 (MQ=255)

                                                gTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATc                                                                                     >  2:4919353/1‑140 (MQ=255)

                                                   aTGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCcaca                                                                                                                           >  2:260783/1‑99 (MQ=255)

                                                   aTGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTc                                                                                  <  1:5804585/140‑1 (MQ=255)

                                                           gCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCag                                                                          >  2:1486678/1‑140 (MQ=255)

                                                           gCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCag                                                                          >  1:2201989/1‑140 (MQ=255)

                                                           gCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCag                                                                          <  1:1974966/140‑1 (MQ=255)

                                                             ttAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCagag                                                                        <  2:5360361/140‑1 (MQ=255)

                                                             ttAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCagag                                                                        <  1:4957109/140‑1 (MQ=255)

                                                              tAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGc                                                                       <  1:978623/140‑1 (MQ=255)

                                                               aaGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCg                                                                      >  2:3725497/1‑140 (MQ=255)

                                                               aaGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCg                                                                      <  1:3895431/140‑1 (MQ=255)

                                                                     aaaTAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCACCGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGt                                                                >  2:101487/1‑140 (MQ=255)

                                                                     aaaTAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGt                                                                >  2:4401088/1‑140 (MQ=255)

                                                                     aaaTAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGt                                                                <  1:4121492/140‑1 (MQ=255)

                                                                     aaaTAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGt                                                                >  1:1712884/1‑140 (MQ=255)

                                                                     aaaTAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGt                                                                <  1:596565/140‑1 (MQ=255)

                                                                     aaaTAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGt                                                                <  2:3446444/140‑1 (MQ=255)

                                                                      aataatCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTa                                                               >  2:4262377/1‑140 (MQ=255)

                                                                      aataatCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTa                                                               >  2:2832453/1‑140 (MQ=255)

                                                                             gtgtCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTaa                                                        <  2:1149091/140‑1 (MQ=255)

                                                                               gtCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTaaaa                                                      >  2:3285780/1‑140 (MQ=255)

                                                                                tCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAAc                                                     >  1:316918/1‑140 (MQ=255)

                                                                                  aCCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAAccc                                                   >  2:5520860/1‑140 (MQ=255)

                                                                                   ccATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCa                                                  <  1:3541900/140‑1 (MQ=255)

                                                                                   ccATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTGTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAAcccc                                                  <  2:1578434/140‑2 (MQ=255)

                                                                                    cATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCAc                                                 <  1:3784519/140‑1 (MQ=255)

                                                                                       tggtggGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACctt                                              <  1:5753090/140‑1 (MQ=255)

                                                                                        ggtggGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACcttc                                             <  1:3995091/140‑1 (MQ=255)

                                                                                        ggtggGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACACACcttc                                             >  2:6093917/1‑140 (MQ=255)

                                                                                         gtggGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACcttct                                            >  2:4490788/1‑140 (MQ=255)

                                                                                         gtggGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACcttct                                            >  1:1454425/1‑140 (MQ=255)

                                                                                          tggGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACcttctt                                           >  1:1821644/1‑140 (MQ=255)

                                                                                           gggTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTta                                          <  1:473860/140‑1 (MQ=255)

                                                                                            ggTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTtat                                         <  1:4490788/140‑1 (MQ=255)

                                                                                              tACTAAACCTGAAGTTCAGCCCACCGGGATGCGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAg                                       >  2:5805790/1‑140 (MQ=255)

                                                                                              tACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAg                                       >  1:608300/1‑140 (MQ=255)

                                                                                              tACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAg                                       >  2:4104134/1‑140 (MQ=255)

                                                                                               aCTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGa                                      <  1:4704934/140‑1 (MQ=255)

                                                                                               aCTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGa                                      <  1:4402491/140‑1 (MQ=255)

                                                                                                cTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGaa                                     >  1:4591362/1‑140 (MQ=255)

                                                                                                   aaCCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCGTATAGAAccc                                  >  2:1740105/1‑140 (MQ=255)

                                                                                                        gAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCa                                                  <  2:4018594/119‑1 (MQ=255)

                                                                                                        gAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCa                                                  >  1:4018594/1‑119 (MQ=255)

                                                                                                        gAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCg                             >  1:2885676/1‑140 (MQ=255)

                                                                                                        gAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCg                             >  1:2775814/1‑140 (MQ=255)

                                                                                                             tCAGCCCACCGGGATGAGAAAAAATCGCCTCCGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGc                        <  1:2585943/140‑1 (MQ=255)

                                                                                                             tCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGc                        >  1:2090025/1‑140 (MQ=255)

                                                                                                             tCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGc                        >  1:2348028/1‑140 (MQ=255)

                                                                                                                gCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCa                     >  1:5045218/1‑140 (MQ=255)

                                                                                                                   cACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCAttt                  >  1:876813/1‑140 (MQ=255)

                                                                                                                     ccGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTg                <  1:1209822/140‑1 (MQ=255)

                                                                                                                      cGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGc               >  1:2436922/1‑140 (MQ=255)

                                                                                                                               aaaaaaTCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACg      >  2:5605551/1‑140 (MQ=255)

                                                                                                                               aaaaaaTCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACg      <  2:3111371/140‑1 (MQ=255)

CGCCAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTGG  >  NZ_CP025268/4215465‑4215735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`