BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,426,312	A→G	229 (TAA→TAG)	ulaF →	L‑ribulose‑5‑phosphate 4‑epimerase UlaF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,426,312	0	A	G	100.0%	1216.0 / NA	350	229 (TAA→TAG)	ulaF	L‑ribulose‑5‑phosphate 4‑epimerase UlaF
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (216/134); total (216/134)

Only 100 of 351 total aligned reads displayed.

TGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGC  >  NZ_CP025268/4426176‑4426446

tggtgATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAac                                                                                                                                      >  1:4335401/1‑140 (MQ=255)

tggtgATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAac                                                                                                                                      >  2:4074506/1‑140 (MQ=255)

  gtgATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAacac                                                                                                                                    >  2:4941678/1‑140 (MQ=255)

   tgATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACAcg                                                                                                                                   >  1:687705/1‑140 (MQ=255)

     aTGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAGACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACAcgcg                                                                                                                                 <  2:149943/140‑1 (MQ=255)

     aTGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACAcgcg                                                                                                                                 >  1:5048513/1‑140 (MQ=255)

        gaagaaGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTg                                                                                                                              >  2:532588/1‑140 (MQ=255)

          agaagTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCg                                                                                                                            >  2:4685597/1‑140 (MQ=255)

          agaagTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCg                                                                                                                            >  2:2126520/1‑140 (MQ=255)

            aagTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGa                                                                                                                          >  2:4703791/1‑140 (MQ=255)

            aagTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGa                                                                                                                          >  2:2255960/1‑140 (MQ=255)

             agTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGaa                                                                                                                         >  1:6015071/1‑140 (MQ=255)

               tGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAAt                                                                                                                       >  1:4149977/1‑140 (MQ=255)

                        tGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTtccttcct                                                                                                              <  2:2062371/140‑1 (MQ=255)

                        tGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTtccttcct                                                                                                              <  1:6141225/140‑1 (MQ=255)

                        tGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTtccttcct                                                                                                              <  1:2934857/140‑1 (MQ=255)

                        tGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTtccttcct                                                                                                              <  2:6297954/140‑1 (MQ=255)

                             tGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGga                                                                                                         <  2:453316/140‑1 (MQ=255)

                                   gctcgcgGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATACACACTTCAGGCGTAAACACGGTCCTACCGCTTATTCCGGGCAGAAGTAGAACACGCGCTGCGGACATTTCCTTCCTCGGGAGATacc                                                                                                   <  2:2700675/137‑3 (MQ=255)

                                   gCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAAc                                                                                                   <  2:1064739/140‑1 (MQ=255)

                                   gCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAAc                                                                                                   <  2:413592/140‑1 (MQ=255)

                                   gCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAAc                                                                                                   <  1:4451775/140‑1 (MQ=255)

                                   gCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAAc                                                                                                   <  2:903998/140‑1 (MQ=255)

                                    cccGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACt                                                                                                  >  2:4474397/1‑140 (MQ=255)

                                              tAACCCACAACTCAATCACATCGACAGATTCCTGATGAATAAACACTTCATGCTTAAACACGTTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTc                                                                                        >  2:2489839/1‑140 (MQ=255)

                                                          cAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGtttt                                                                            >  2:3488968/1‑140 (MQ=255)

                                                           aaTCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGttttt                                                                           >  2:3148963/1‑140 (MQ=255)

                                                           aaTCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGttttt                                                                           >  2:776013/1‑140 (MQ=255)

                                                           aaTCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGttttt                                                                           >  1:4546719/1‑140 (MQ=255)

                                                            aTCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTc                                                                          >  2:4433546/1‑140 (MQ=255)

                                                            aTCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTc                                                                          >  1:2225025/1‑140 (MQ=255)

                                                                caTCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCaaaa                                                                      >  2:1924125/1‑140 (MQ=255)

                                                                  tCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCaaaaaa                                                                    >  1:3661862/1‑140 (MQ=255)

                                                                  tCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCaaaaaa                                                                    >  1:1011996/1‑140 (MQ=255)

                                                                      cAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGc                                                                <  1:262489/140‑1 (MQ=255)

                                                                      cAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGc                                                                <  2:1289868/140‑1 (MQ=255)

                                                                        gCTTCCTGATGAATAAACACTTCATGCGTAAACAAGGTCCTAACGCTTATTACGGGCAGAATTAGAACACGCTCTGCTGAAATTTACTTCATCTGGAGATAACTTGTCTAATTCCGCAGCAGTTTTTCAAAAAAAAGccc                                                              <  2:2553443/140‑1 (MQ=255)

                                                                           tCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGATTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑t                                                          >  2:5179158/1‑140 (MQ=255)

                                                                               gatgaATAAACACATCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAACAGTTTTTCAAAAAAAAGCCCCC‑TGCGa                                                      >  1:5655092/1‑140 (MQ=255)

                                                                                ctgaATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGaa                                                     <  2:2417401/139‑1 (MQ=255)

                                                                                   aaTAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAggg                                                  >  1:3202620/1‑140 (MQ=255)

                                                                                    aTAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAgggg                                                 >  1:5793282/1‑140 (MQ=255)

                                                                                     tAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGAAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAggggg                                                <  2:245644/140‑1 (MQ=255)

                                                                                       aaCACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGgca                                              >  1:1547989/1‑140 (MQ=255)

                                                                                          acTTCATGCGTACACACGGTCCTAACGATTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTGCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTAGTTCAAAAAAAAGCCCCC‑TGCGAAGGGGgcaaag                                           <  2:6202336/140‑1 (MQ=255)

                                                                                          acTTCATGCGTAAACACGGTGCTCACGGTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAACGTTCCTTCCTCGGGAGATTACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGTCGGGGgctaag                                           >  2:6032443/1‑140 (MQ=255)

                                                                                            ttCATGCGTAAACACGGTCCTAACGCTTATTCCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCGTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGgcaaagca                                         <  1:844147/140‑1 (MQ=255)

                                                                                            ttCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCTGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGgcaaagca                                         <  1:1470997/140‑1 (MQ=255)

                                                                                            ttCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGgcaaagca                                         <  2:5499540/140‑1 (MQ=255)

                                                                                            ttCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTGTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGgcaaagca                                         >  1:4069572/1‑140 (MQ=255)

                                                                                            ttCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCGTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGgcaaagca                                         <  2:1768527/140‑1 (MQ=255)

                                                                                             tCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGgcaaagcaa                                        >  1:2457674/1‑140 (MQ=255)

                                                                                             tCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGgcaaagcaa                                        >  1:6351726/1‑140 (MQ=255)

                                                                                             tCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGgcaaagcaa                                        >  1:1607385/1‑140 (MQ=255)

                                                                                             tCATGCGTAAACACGGTCATAACGCTTATTACGGGCAGAAGTAGAACACGAGCTGCGGAAATTTCCATCCTCGGGAGATAACTGGTCTAATTCCGCTGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGgcaaagcaa                                        >  2:6247065/1‑140 (MQ=255)

                                                                                               aTGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAAc                                      <  1:3568653/140‑1 (MQ=255)

                                                                                                tGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACt                                     >  1:1206708/1‑140 (MQ=255)

                                                                                                tGCGTAAACACGGTCCTAACGCTAATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCATCCGTTTTTCAAAAAAAAGCCCCC‑AGCGAAGGGGGCAAAGCAAACt                                     >  2:5024051/1‑140 (MQ=255)

                                                                                                 gCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTa                                    <  1:1962541/140‑1 (MQ=255)

                                                                                                 gCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTa                                    <  1:4772571/140‑1 (MQ=255)

                                                                                                 gCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTa                                    <  1:4026689/140‑1 (MQ=255)

                                                                                                      aaCACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAATCCCCCGTGGGAAGGGGgcaatgcaaaaattgcc                                >  1:2133265/1‑133 (MQ=255)

                                                                                                      aaCACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCa                               >  2:709035/1‑140 (MQ=255)

                                                                                                      aaCACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCa                               >  1:304581/1‑140 (MQ=255)

                                                                                                      aaCACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCa                               >  2:2022750/1‑140 (MQ=255)

                                                                                                       acacGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCaa                              <  1:6268016/140‑1 (MQ=255)

                                                                                                           ggTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGtt                          >  2:3753194/1‑140 (MQ=255)

                                                                                                            gTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATCACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGttt                         >  1:1868032/1‑140 (MQ=255)

                                                                                                             tCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTc                        >  1:1380134/1‑140 (MQ=255)

                                                                                                             tCCTAACGCTGATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTc                        >  2:3421681/1‑140 (MQ=255)

                                                                                                              ccTAACGCTTTTTACGGGCAGAAGTAGAACACGCGCTGCGGATAGTTCCGTCCTCGGGAGATTAATGTTCTTATTCCGCAGCCGTTTTTCTAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCg                       <  1:5379264/140‑1 (MQ=255)

                                                                                                               cTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGt                      <  2:2977710/140‑1 (MQ=255)

                                                                                                               cTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGt                      <  2:5934092/140‑1 (MQ=255)

                                                                                                               cTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGt                      <  1:1173446/140‑1 (MQ=255)

                                                                                                               cTAACGCTTATTACGGGCAGAAGTAGAACACGCGATGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCTTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAACCTATGGCAATGTTTCGt                      <  2:2908989/140‑1 (MQ=255)

                                                                                                                 aaCGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTg                    >  1:4930216/1‑140 (MQ=255)

                                                                                                                    gCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGtt                 <  1:785728/140‑1 (MQ=255)

                                                                                                                     cTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTATTTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCCAACTATGGCAATGTTTCGTTGGTta                <  2:5117993/140‑1 (MQ=255)

                                                                                                                     cTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTta                <  2:2803104/140‑1 (MQ=255)

                                                                                                                     cTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAAATCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTta                >  2:2177392/1‑140 (MQ=255)

                                                                                                                       tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATCACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTAAGGCAATGTTTCGTTGGTtata              >  2:4225753/1‑140 (MQ=255)

                                                                                                                       tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAATAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTtata              >  2:4284223/1‑140 (MQ=255)

                                                                                                                       tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTtata              >  2:5592883/1‑140 (MQ=255)

                                                                                                                       tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTtata              >  1:1002655/1‑140 (MQ=255)

                                                                                                                       tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTtata              >  1:1834632/1‑140 (MQ=255)

                                                                                                                       tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTtata              >  2:395354/1‑140 (MQ=255)

                                                                                                                       tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTtata              >  2:1609723/1‑140 (MQ=255)

                                                                                                                       tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTtata              >  1:2527992/1‑140 (MQ=255)

                                                                                                                       tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTtata              >  1:2799484/1‑140 (MQ=255)

                                                                                                                       tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTtata              >  1:5559759/1‑140 (MQ=255)

                                                                                                                       tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTtata              >  1:5147502/1‑140 (MQ=255)

                                                                                                                       tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTtata              >  1:3314305/1‑140 (MQ=255)

                                                                                                                       tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTtata              >  1:3789490/1‑140 (MQ=255)

                                                                                                                       tattaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGAAAAGCAAACTATGGCAATGTTTCGTTGGTtata              >  1:2015798/1‑140 (MQ=255)

                                                                                                                        attaCGGTCAGAAGTAGAACACGCGCTGCGGAAATTTCGTTCCTCGGTTGATAACTTGTCTAATTCCGAAGCCGTTTTTCAAAAAATAGCCACC‑TTCGATGGGTGCAAAGCAATCTATGGAAATGTTTCGTTGGTtattc             >  2:534270/1‑138 (MQ=255)

                                                                                                                         tgaCGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTACAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATAcc            <  1:957447/138‑1 (MQ=255)

                                                                                                                              ggCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGgtg       >  2:3595836/1‑140 (MQ=255)

                                                                                                                              ggCAGAAGTAGAACACGCGCTGCGGAAATTTCCGACCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGgtg       <  2:1369099/140‑1 (MQ=255)

                                                                                                                                  gAAGTAGAACACGCGCTGCGGAAATGTCCGTCCTCGGGAGATCACTGGTCTAATTCCGCAGACGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAg   <  2:388519/140‑1 (MQ=255)

                                                                                                                                   aaGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGc  >  2:5796748/1‑140 (MQ=255)

TGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCC‑TGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGC  >  NZ_CP025268/4426176‑4426446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`