Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 489,335 T→C *176* (TAA→TAG)  priC ← primosomal replication protein N''

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268489,3350TC99.4% 1236.1 / NA 355*176* (TAA→TAG) priCprimosomal replication protein N''
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (241/112);  minor base A (2/0);  total (243/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 355 total aligned reads displayed.

ATAATCACGCTTTACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGG  >  NZ_CP025268/489199‑489471
                                                                                                                                        |                                                                                                                                        
    tCACGCTTTACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCggg                                                                                                                                   >  1:1906791/1‑140 (MQ=255)
       cGCTTTACAAGATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCAATTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTa                                                                                                                                <  1:4431181/140‑1 (MQ=255)
        gCTTTACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCAGATTTTCCTCCACCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTGCCAGTGCCATATTCTCTCCAGTGCTAGCGGGTTaa                                                                                                                               <  2:331119/140‑1 (MQ=255)
                  atCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAAc                                                                                                                     >  2:5830076/1‑140 (MQ=255)
                    ccAGCGCGCGCCGCACGGTACTGGCAGGGATCTGATTTTCCTCCTGCAGCACACTCAAATCGACAGCCAGGGTGACATCGTCAGGGGCATTTTCCCGTGACGTATTCTCGCCAGTGCTAGCGGGTGAAACgcgcgacccg                                                                                                                   >  2:2763965/1‑134 (MQ=255)
                    ccAGCGCGCGCAGCACGGTACTGGCCGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACAGCGTCAGGGGCATTTTCCAGTGACATATTCTCTCAATTGCTAGCGGGTTAAACGCGCTAAccg                                                                                                                   >  2:5575355/1‑139 (MQ=255)
                    ccAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCt                                                                                                                   >  1:3612624/1‑140 (MQ=255)
                    ccAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCt                                                                                                                   >  2:5082417/1‑140 (MQ=255)
                    ccAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCt                                                                                                                   >  2:5444712/1‑140 (MQ=255)
                      aGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTCACCTgg                                                                                                                 >  1:6251038/1‑139 (MQ=255)
                      aGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGt                                                                                                                 >  2:4282763/1‑140 (MQ=255)
                      aGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGt                                                                                                                 >  1:870188/1‑140 (MQ=255)
                      aGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGt                                                                                                                 >  2:5363157/1‑140 (MQ=255)
                      aGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAAACGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCCGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTCACCTGt                                                                                                                 >  2:345878/1‑140 (MQ=255)
                        cgcgcgcAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCCTTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGttt                                                                                                               >  1:1025488/1‑140 (MQ=255)
                        cgcgcgcAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGttt                                                                                                               >  2:3099829/1‑140 (MQ=255)
                        cgcgcgcAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGttt                                                                                                               >  1:4925298/1‑140 (MQ=255)
                        cgcgcgcAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGttt                                                                                                               >  1:4530384/1‑140 (MQ=255)
                        cgcgcgcAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGttt                                                                                                               >  1:3945173/1‑140 (MQ=255)
                            cgcAGCAACGTACAGGCAGGGATCTGAATTTCCTCCAGCAGCACACTCACTTCGACAGACAGTATGACAACGTCAGGGGCATTTTCCAGTGACATATTATCTCCATTGCTAGCGAGTAAAACCCGCTAACCTGTTTTcta                                                                                                           >  2:4093543/1‑138 (MQ=255)
                             gcagcaCGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGAt                                                                                                          >  1:2643826/1‑140 (MQ=255)
                                     gTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGGGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCa                                                                                                  >  2:563636/1‑140 (MQ=255)
                                      tACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAg                                                                                                 >  1:908539/1‑140 (MQ=255)
                                      tACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAg                                                                                                 <  1:5574644/140‑1 (MQ=255)
                                      tACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAAGCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAg                                                                                                 <  2:5933880/140‑1 (MQ=255)
                                      tACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATAAAATCTACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTTCTAGCGGGTTAAACGAGCTAAACTGTTTTCGATTTTTTCCAg                                                                                                 <  1:527840/140‑1 (MQ=255)
                                        cTGCCAGCGAACTGAATTTCCTCCAGCCGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCAATTACCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATATTATCCAgcg                                                                                               <  2:12142/140‑1 (MQ=255)
                                         tGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCNTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAgcgc                                                                                              >  1:5703905/1‑140 (MQ=255)
                                         tGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAgcgc                                                                                              >  1:2916761/1‑140 (MQ=255)
                                          ggCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCa                                                                                             <  2:3571709/140‑1 (MQ=255)
                                              gggATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggc                                                                                         >  2:1148057/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCTATTTTTTCCAGCGCATggcgg                                                                                       >  1:4478846/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCTTggcgg                                                                                       >  1:4063844/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  1:1626102/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  2:371492/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  1:2518738/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  2:5879744/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  2:479806/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  2:4684791/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  1:6103664/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  2:2116377/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  1:1812319/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  1:5516118/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  1:1372804/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  2:4461451/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  1:4820693/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  1:3474357/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  1:4588533/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  1:3561252/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGGGACCTATTCTCTCCATTGCTAGCGGGTTAAACGCGCTACCCTGGTTTCGATTTTTTCCAGCGCCTggcgg                                                                                       >  1:5818092/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTACAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  2:460765/1‑140 (MQ=255)
                                                  tCTGATTGTCCTCCAGCAGCACAATCAAATCGGCAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACCTATTCTCTCCCTGGCTAGCGGGTTAAGCGCGCTACCCTGGTTTCGATTTTTTCCAGCGCATGGCGGCa                                                                                     >  2:1502150/1‑140 (MQ=255)
                                                   cTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGCCATCGTCAGGGGCATTTTCCAGTGACATATTCACTCCATTGCTAGCGGGTTAAACGCGCGAACCTGATTTCGATTTTTTCCAGCGCATGGCGGCAg                                                                                    <  2:3866820/140‑1 (MQ=255)
                                                          tcctccAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCa                                                                             >  1:4305588/1‑140 (MQ=255)
                                                             tccAGCAGCACAATCAAATCGACAGCCAGTTTGACGTCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAAGCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgc                                                                          >  2:6038784/1‑140 (MQ=255)
                                                              ccAGCAGCACAATCCAATCGACAGCCAGTTTGACATCGTCAGGGGCAATTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgcg                                                                         <  2:574590/140‑1 (MQ=255)
                                                              ccAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgcg                                                                         <  2:6316192/140‑1 (MQ=255)
                                                                cgcagcaCAATCAACTCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGCCATATTCTCTCCAGTGCGAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCTCATGGCGGCAGCGTGCCAGgcgcg                                                                       <  2:2391428/139‑1 (MQ=255)
                                                                agcagcACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgcgcg                                                                       >  1:6312008/1‑140 (MQ=255)
                                                                agcagcACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgcgcg                                                                       <  2:656539/140‑1 (MQ=255)
                                                                agcagcACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgcgcg                                                                       >  1:2828841/1‑140 (MQ=255)
                                                                agcagcACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCAGTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgcgcg                                                                       >  1:275299/1‑140 (MQ=255)
                                                                     cacaATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTCACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCa                                                                  >  1:3315857/1‑140 (MQ=255)
                                                                     cacaATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCa                                                                  <  2:5256303/140‑1 (MQ=255)
                                                                        aaTCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAg                                                               <  1:5444712/140‑1 (MQ=255)
                                                                          tCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGc                                                             <  1:2800509/140‑1 (MQ=255)
                                                                          tCAAAGCGACAGCCAGTTTGACATCGTCAGGGGCAAATTACAGTGACATATTCTCTCAATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATATTTTCCAGCGCATGGCTGCAGCGTGCCAGGCGCGCTTCATAggg                                                             >  2:396541/1‑139 (MQ=255)
                                                                          ccacaTCGCCCGCCACTTTGCCATCGTCAGGGGCAATTTCCCCTGACATAATCTCTCCAGTGCTACCGCGTGAACCGCGCTAACCAGTCTTCGCTATATTCCAGCGCACGGCGGCAGCGTGCCAGGCGCGCTTCATCGGc                                                             <  2:1713162/136‑1 (MQ=255)
                                                                               tCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCa                                                        <  2:3612624/140‑1 (MQ=255)
                                                                               tCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCa                                                        >  1:2350096/1‑140 (MQ=255)
                                                                               tCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCa                                                        >  1:5788940/1‑140 (MQ=255)
                                                                               tCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGGTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCa                                                        >  1:3720282/1‑140 (MQ=255)
                                                                                  aCAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACtt                                                     >  1:1798592/1‑140 (MQ=255)
                                                                                   cAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTc                                                    <  2:2846702/140‑1 (MQ=255)
                                                                                    aGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCa                                                   <  2:842766/140‑1 (MQ=255)
                                                                                    aGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCa                                                   <  1:1263442/140‑1 (MQ=255)
                                                                                    aGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCa                                                   >  2:1333397/1‑140 (MQ=255)
                                                                                                  cGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTg                                     <  2:3217812/140‑1 (MQ=255)
                                                                                                  cGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTg                                     <  1:5133249/140‑1 (MQ=255)
                                                                                                  cGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTg                                     >  2:181424/1‑140 (MQ=255)
                                                                                                   gTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGc                                    >  2:6199856/1‑140 (MQ=255)
                                                                                                   gTCAGGGGCACGTTCCAGTGACAGATACTCTCCAGTGCTAGCGGGTTAAACGCGCTACCCAGATTGCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCCCGATGCAGCGTTTGc                                    <  1:2097416/140‑1 (MQ=255)
                                                                                                     cAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTg                                  <  1:1114921/140‑1 (MQ=255)
                                                                                                      aGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGt                                 >  1:647486/1‑140 (MQ=255)
                                                                                                          gCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCa                             <  1:6013735/140‑1 (MQ=255)
                                                                                                          gCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCa                             <  2:1882803/140‑1 (MQ=255)
                                                                                                             ttttCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACga                          <  2:3042073/140‑1 (MQ=255)
                                                                                                             ttttCCAGTGACATATTCTCTCCAGTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATCGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACga                          <  1:1497401/140‑1 (MQ=255)
                                                                                                                   aGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCgg                    <  2:1558929/140‑1 (MQ=255)
                                                                                                                     tGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTc                  >  1:4738062/1‑140 (MQ=255)
                                                                                                                     tGACATATTCTCTCCATTGCTAGCGGGGTAAACGCGCGAACCTGTGTTCGATTGTTTGCAGCGCATGGCGGCAGGGTGCCTGGCGCGCTGCATAGGCGTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTc                  >  2:2584202/1‑140 (MQ=255)
                                                                                                                       aCATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCAc                <  2:5703905/140‑1 (MQ=255)
                                                                                                                        cATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCAcc               >  2:3751118/1‑140 (MQ=255)
                                                                                                                        cATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCAcc               <  1:4190141/140‑1 (MQ=255)
                                                                                                                           atTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCcgc            >  2:4200344/1‑140 (MQ=255)
                                                                                                                               tctcCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCa        >  1:255500/1‑140 (MQ=255)
                                                                                                                                 tcCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGa      <  2:4768839/140‑1 (MQ=255)
                                                                                                                                 tcCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGa      >  1:3205194/1‑140 (MQ=255)
                                                                                                                                 tcCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGa      >  1:2233987/1‑140 (MQ=255)
                                                                                                                                 tcCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGa      >  2:4154346/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                        
ATAATCACGCTTTACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGG  >  NZ_CP025268/489199‑489471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑