BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	356,677	2 bp→CC	intergenic (+172/+164)	codA → / ← cynR	cytosine/isoguanine deaminase/transcriptional regulator CynR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	356,677	0	T	C	96.9%	859.5 / NA	299	intergenic (+172/+165)	codA/cynR	cytosine/isoguanine deaminase/transcriptional regulator CynR
Reads supporting (aligned to +/- strand): ref base T (1/4); major base C (89/194); minor base A (8/1); total (100/199)
Fisher's exact test for biased strand distribution p-value = 7.89e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NZ_CP025268	356,678	0	A	C	95.6%	885.3 / 10.1	299	intergenic (+173/+164)	codA/cynR	cytosine/isoguanine deaminase/transcriptional regulator CynR
Reads supporting (aligned to +/- strand): ref base A (8/5); new base C (92/193); total (100/199)
Fisher's exact test for biased strand distribution p-value = 3.72e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 299 total aligned reads displayed.

CGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGG  >  NZ_CP025268/356546‑356812

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  tGAACGCCTTATCCGGCCTGCGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGcc                                                                                                                               <  2:596119/140‑1 (MQ=11)

     aCGCCTTATCCGGCCTACGTAGAGCACTGACCTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACCCCCGCGCATTCCTCCAACGCCAtg                                                                                                                            <  2:4394486/140‑1 (MQ=11)

              ccGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAAt                                                                                                                   >  1:5085259/1‑140 (MQ=255)

                ggCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGc                                                                                                                 <  2:5877275/140‑1 (MQ=255)

                  ccTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCt                                                                                                               >  2:4920474/1‑140 (MQ=255)

                  ccTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCt                                                                                                               >  1:33971/1‑140 (MQ=255)

                   cTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCtt                                                                                                              <  2:6305958/140‑1 (MQ=255)

                           agCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTAATCCAACGCCATGTGCAAAAATGcc                                                                                                                >  1:103085/1‑130 (MQ=25)

                           agCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGcc                                                                                                                >  1:4726908/1‑130 (MQ=255)

                           agCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTACTCCAACGCCATGTGCAAAAATGCCTTCGcggcgg                                                                                                      >  1:1527267/1‑140 (MQ=11)

                            gCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTACCGCCAACAACCGCGCATTCATCCAACGCCATGTGCAAAAATGCCTTCGCggcggc                                                                                                     >  1:4887812/1‑140 (MQ=255)

                             cACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCt                                                                                                    >  2:1098667/1‑140 (MQ=255)

                               cTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTACTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGt                                                                                                  >  2:3571538/1‑140 (MQ=255)

                                tGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTc                                                                                                 >  1:4806631/1‑140 (MQ=255)

                                 gAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCt                                                                                                >  1:3425451/1‑140 (MQ=11)

                                   aCTCGTAGGCCTGATAAGCGTAGCGCCTCAGGCAAGTCCAGCCGCTGCTCTGTGTCAGCGGCTACCCTGAGTCCGTCCCGCCAACCACCGCGCATTCCCCCCCCGCCATGTGCCCACACGCCTTCGCAGCGGCTGACTGc                                                                                              <  2:1531690/140‑1 (MQ=2)

                                   aCTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGc                                                                                              <  1:5432745/140‑1 (MQ=255)

                                    cTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGcc                                                                                             <  1:4879024/140‑1 (MQ=255)

                                    cTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGcc                                                                                             <  2:2561806/140‑1 (MQ=255)

                                    cTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGcc                                                                                             <  1:3766087/140‑1 (MQ=255)

                                        tAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCTGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCt                                                                                         <  1:171405/140‑1 (MQ=11)

                                          ggCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGt                                                                                       >  2:5316682/1‑140 (MQ=255)

                                                    gCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGcc                                                                             <  1:500572/140‑1 (MQ=255)

                                                     cGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACCACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTGTAGTTGAGGCCg                                                                            >  1:3590486/1‑140 (MQ=11)

                                                     cGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCg                                                                            >  1:3294650/1‑140 (MQ=255)

                                                     cGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTCTTGttcaggcgc                                                                            >  2:2882416/1‑133 (MQ=11)

                                                           gcATCAGGCACGTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCAGTCCCGCCAACACCCGCGCATTCCTCCACCGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATgcg                                                                      <  2:3996251/140‑1 (MQ=17)

                                                           gcATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATgcg                                                                      <  1:4309155/140‑1 (MQ=255)

                                                              tCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATgcggcg                                                                   >  1:3619123/1‑140 (MQ=255)

                                                                aGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTg                                                                 <  1:737338/140‑1 (MQ=255)

                                                                aGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTg                                                                 <  1:3231102/140‑1 (MQ=255)

                                                                aGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCAGTCCCGCCACCAACCGCGCATTCCTCCAACGCCATGTGCAACAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTg                                                                 <  2:6336688/140‑1 (MQ=255)

                                                                aGGCAATGCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATACAGTCCCGCCAACAACCGCGCATTCCTCCAACTCCATGTGCAAACATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGACGGATGCGGCGTg                                                                 <  2:192004/140‑1 (MQ=255)

                                                                 ggCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAACATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGa                                                                <  1:2894626/140‑1 (MQ=18)

                                                                 ggCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGa                                                                <  1:6427743/140‑1 (MQ=255)

                                                                 ggCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGa                                                                <  1:3538056/140‑1 (MQ=255)

                                                                 ggCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGa                                                                <  2:315152/140‑1 (MQ=255)

                                                                 ggCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCAGTCCCGCCACCAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGa                                                                <  2:5823331/140‑1 (MQ=18)

                                                                 cgCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCAGTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGa                                                                <  2:1714113/139‑1 (MQ=18)

                                                                     aTTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACACCCGCGCAGTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGc                                                            <  2:199954/140‑1 (MQ=18)

                                                                     aTTCCAGCCGCTGAGCTGGGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTACTCCAACGCCATGTGCAAAAATGCCTTCGCCGCGGCTGTCTGCCAGCTCTAGTTTAGGCCGGAAGCGGCCTGACCGc                                                            >  2:3363868/1‑140 (MQ=14)

                                                                       tCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCt                                                          >  2:2714452/1‑140 (MQ=255)

                                                                       tCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATCCAGGAAACGGCGTGAACGcca                                                          >  2:3500841/1‑139 (MQ=17)

                                                                          aGCCGCTGCTCTGTGTCAGCGGCTACCGAGAGTCATTCCCGCCCCCACCCGCGCATTCCTCCAACGCCATGTGCACAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTag                                                       <  2:5663412/140‑2 (MQ=255)

                                                                          aGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTAt                                                       <  1:580517/140‑1 (MQ=255)

                                                                          aGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTAt                                                       <  2:5835018/140‑1 (MQ=255)

                                                                          aGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTAt                                                       <  1:5263681/140‑1 (MQ=255)

                                                                          aGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCAGTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTAt                                                       <  2:323408/140‑1 (MQ=21)

                                                                          aGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCAGTCCTCCAACGCCATGTGCAAAAATGCATTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTAt                                                       <  2:3731229/140‑1 (MQ=21)

                                                                            ccGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATcc                                                     >  2:2113116/1‑140 (MQ=255)

                                                                              gCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCgg                                                   >  1:6338861/1‑140 (MQ=255)

                                                                               cggATCTGTGTCAGCGGCTACCGTGATTCCGACCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                  <  2:2990152/138‑1 (MQ=21)

                                                                               cggATCGGGGTCAGCGGCTACCGTGATTCATTCCCGCCAACCACCGCGCAGTCCTCCAACGCCATGTGCAACCATGCCTTCGCAGCGGCTGTCTGCCCGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                  <  2:4227545/138‑1 (MQ=37)

                                                                               cTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCTAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                  <  2:1289556/140‑1 (MQ=39)

                                                                               cTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                  <  2:4693337/140‑1 (MQ=255)

                                                                               cTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                  <  1:972674/140‑1 (MQ=255)

                                                                               cTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                  <  2:2151210/140‑1 (MQ=255)

                                                                               cTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                                  <  1:4004598/140‑1 (MQ=255)

                                                                                tGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTCGTTTATGCCGGATGCGGCGTGACCGCCTTATCCGGcc                                                 >  1:6225883/1‑140 (MQ=18)

                                                                                 gATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCt                                                <  1:2348175/140‑1 (MQ=255)

                                                                                 gATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCt                                                <  2:2838410/140‑1 (MQ=255)

                                                                                 gATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCt                                                <  2:6245399/140‑1 (MQ=255)

                                                                                    cTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACg                                             <  1:5316682/140‑1 (MQ=255)

                                                                                    cTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACg                                             <  1:5743873/140‑1 (MQ=255)

                                                                                      gtgtCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTa                                           <  1:6273469/140‑1 (MQ=255)

                                                                                       tgtCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGGGAACGCCTTATCCGGCCTACGTag                                          >  1:374368/1‑140 (MQ=21)

                                                                                          cAGCGGCTACCGAGAGTCATGCCCGCCCACCCCCGCGCATGCCTCCACCGCCATGGGCAAAAATGCCGTCGCAGCGGCTGTCGGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCAGGCCTACgagttca                                       <  2:5779231/140‑7 (MQ=19)

                                                                                           agctgctACCTTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTTCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCa                                      <  1:6082770/134‑1 (MQ=37)

                                                                                           aGCGGCTACCGTGATTCATTCCCGCCAACACCCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCa                                      <  1:453894/140‑1 (MQ=25)

                                                                                           aGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCCACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCa                                      <  1:4453107/140‑1 (MQ=25)

                                                                                            gCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCAc                                     >  1:2863651/1‑140 (MQ=255)

                                                                                             cGGCTACCGTGATTCCTTCCCGCCAACAACCGCGCATTCCTCCCACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACt                                    <  2:4441785/140‑1 (MQ=25)

                                                                                              ggctACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTg                                   <  2:4842420/137‑1 (MQ=255)

                                                                                              ggctACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCATATCCGGCCTACGTAGAGCACTg                                   >  1:2237527/4‑140 (MQ=25)

                                                                                              ggctACCGTGATTCAGTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAACTGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGAAGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTg                                   <  1:5605776/137‑1 (MQ=37)

                                                                                               gctACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAACAATGCCTTCGCAGCGGCTGTCTGCCAGCAGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGa                                  <  2:3821677/138‑1 (MQ=25)

                                                                                               gctACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGa                                  <  1:1222926/138‑1 (MQ=255)

                                                                                               gctACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGa                                  <  1:4959290/138‑1 (MQ=255)

                                                                                                 tACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCTGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGAAGCGGCGTGAACTCATTATCCGGCCTACGTAGAGCACTGaaa                                >  2:716640/1‑139 (MQ=21)

                                                                                                 tACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAAc                                >  1:1616188/1‑140 (MQ=255)

                                                                                                 tACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAAc                                >  2:2192733/1‑140 (MQ=255)

                                                                                                 tACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAAc                                >  1:5968750/1‑140 (MQ=255)

                                                                                                    cGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCg                             <  1:3922110/140‑1 (MQ=255)

                                                                                                    cGTGATTCAGTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCg                             <  2:33971/140‑1 (MQ=255)

                                                                                                      tGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTa                           <  2:5201946/140‑1 (MQ=255)

                                                                                                      tGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGCCCTACGTAGAGCACTGAACTCGTa                           <  2:121295/140‑1 (MQ=255)

                                                                                                      tGAGTCATTCCCGCCAACACCCGCGCAGTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCGGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTa                           <  1:1467471/140‑1 (MQ=25)

                                                                                                       gATTCATTCCCGCCAACAACCGCGCAGTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAg                          <  1:1956867/140‑1 (MQ=255)

                                                                                                        attcattcCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAgg                         >  2:2176103/1‑140 (MQ=255)

                                                                                                         ttCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGc                        <  1:2714452/140‑1 (MQ=255)

                                                                                                         ttCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGc                        <  1:2074753/140‑1 (MQ=255)

                                                                                                         ttCAGTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGc                        <  2:1872633/140‑1 (MQ=255)

                                                                                                             ttcccGCCAACAACCGCGCAGTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGa                    <  1:2611359/137‑1 (MQ=255)

                                                                                                              tcccGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAACTGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGAt                   <  2:2275859/138‑1 (MQ=255)

                                                                                                              tcccGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGAt                   <  2:272542/138‑1 (MQ=255)

                                                                                                                  gCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGc               >  1:3068104/1‑140 (MQ=255)

                                                                                                                   cccacaccCGCGCAGTCCTCCAACGCCATGGGCAAAAATGCCTTCGCAGCGGCTGACTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGTCGCGCACTGAACTCGTAGGCCTGATAAGCg              <  1:1831687/137‑1 (MQ=21)

                                                                                                                   ccAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCt                                                                                         <  1:84003/65‑1 (MQ=255)

                                                                                                                   ccAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCt                                                                                         >  2:84003/1‑65 (MQ=255)

                                                                                                                               cATATATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACGCGTAGGCCTGATAAGCGTAGCGCATCAgg  <  1:2023198/140‑1 (MQ=255)

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CGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGG  >  NZ_CP025268/356546‑356812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`