Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,512,432 (A)9→8 pseudogene (599/907 nt) CXP41_RS18440 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,512,4240A.99.2% 1698.8 / 11.3 365pseudogene (607/907 nt)CXP41_RS18440hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base . (220/136);  total (224/141)
Fisher's exact test for biased strand distribution p-value = 5.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 387 total aligned reads displayed.

CGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAGCGCG  >  NZ_CP025268/3512286‑3512560
                                                                                                                                          |                                                                                                                                        
cggcgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCaa                                                                                                                                         >  2:898492/1‑140 (MQ=255)
cggcgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCaa                                                                                                                                         <  2:188303/140‑1 (MQ=255)
  gcgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCaaaa                                                                                                                                       >  2:4728622/1‑140 (MQ=255)
   cgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCaaaaa                                                                                                                                      >  2:792428/1‑140 (MQ=255)
   cgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCaaaaa                                                                                                                                      >  1:4905025/1‑140 (MQ=255)
   cgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCaaaaa                                                                                                                                      >  2:1743700/1‑140 (MQ=255)
    gTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCaaaaaa                                                                                                                                     >  2:1614554/1‑140 (MQ=255)
         ctctAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCaaaaaaaacat                                                                                                                                <  2:2153686/140‑4 (MQ=255)
              aTCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTc                                                                                                                          <  2:2384075/140‑1 (MQ=255)
               tCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCa                                                                                                                         <  1:4119844/140‑1 (MQ=255)
                 ccAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATg                                                                                                                       <  2:4279632/140‑1 (MQ=255)
                   aGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGAt                                                                                                                     >  1:1703804/1‑140 (MQ=255)
                   aGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGAt                                                                                                                     >  2:2477265/1‑140 (MQ=255)
                   aGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGAt                                                                                                                     >  2:3490968/1‑140 (MQ=255)
                   aGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGAt                                                                                                                     >  1:2160833/1‑140 (MQ=255)
                   aGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGAt                                                                                                                     >  1:4780113/1‑140 (MQ=255)
                   aGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGAt                                                                                                                     >  2:1025132/1‑140 (MQ=255)
                   aGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGAt                                                                                                                     >  1:4973586/1‑140 (MQ=255)
                   aGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCCTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGaa                                                                                                                     >  2:1184487/1‑139 (MQ=255)
                     cATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGAttt                                                                                                                   >  1:1597732/1‑140 (MQ=255)
                        ggTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCt                                                                                                                >  1:4388004/1‑140 (MQ=255)
                        ggTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCt                                                                                                                >  1:4436391/1‑140 (MQ=255)
                          tCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGa                                                                                                              >  2:481739/1‑140 (MQ=255)
                          tCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGa                                                                                                              >  1:2794493/1‑140 (MQ=255)
                          tCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATCCGTTGTAAACTCTCGGGATAAGATAGATCGACTCGTTCCACATCACTACC‑CCCAAAAAAAACATCGCTCCTGATTTTccac                                                                                                              >  2:3087486/1‑137 (MQ=255)
                            aGTTCGTTTTCCGCCCCCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTACACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATc                                                                                                            <  1:2348061/140‑1 (MQ=255)
                              ttCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACGTCACTACCGCC‑AAAAAAAACATCGGTCATGACTTTCTGATcct                                                                                                          >  2:4503097/1‑139 (MQ=255)
                              ttCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATccg                                                                                                          >  1:125762/1‑140 (MQ=255)
                                 gTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAGAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                       <  1:273566/140‑1 (MQ=255)
                                 gTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                       <  1:2957269/140‑1 (MQ=255)
                                 gTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                       <  1:4603154/140‑1 (MQ=255)
                                 cttttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                       <  1:579883/139‑1 (MQ=255)
                                   tttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGaa                                                                                                     <  1:5110441/140‑1 (MQ=255)
                                      ccGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAATAGAGATCGCCGCGTTCCACATCACTACCG‑CGAAAAAAATCATCGGTCATGATTTTTTGATCCGCCGAACgg                                                                                                  <  2:4883440/140‑1 (MQ=255)
                                      ccGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                                  <  1:2651058/140‑1 (MQ=255)
                                      ccGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                                  <  1:1873839/140‑1 (MQ=255)
                                      ccGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                                  <  2:616774/140‑1 (MQ=255)
                                        gCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTATCGCC‑AAAAAAAACAGCTGTCTTGATTTTCTGATCCGCCGTACGGtt                                                                                                >  2:1461436/1‑140 (MQ=255)
                                           accacGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATc                                                                                             <  1:4025838/140‑1 (MQ=255)
                                             cacGTTCCAGATCAACGCATCGACATCGCCTTTAACAATGCGTTGTAACCTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCa                                                                                           <  2:3170216/140‑1 (MQ=255)
                                                     aGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                                   <  1:3254167/140‑1 (MQ=255)
                                                     aGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                                   <  2:2741274/140‑1 (MQ=255)
                                                          aCCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt                                                                              >  1:3860402/1‑140 (MQ=255)
                                                           ccGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTGATCCAGCCCCACGCGCTTc                                                                             >  1:2076568/1‑140 (MQ=255)
                                                            cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCa                                                                            >  2:3887782/1‑140 (MQ=255)
                                                            cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCa                                                                            >  1:847107/1‑140 (MQ=255)
                                                              cATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACg                                                                          >  1:2450429/1‑140 (MQ=255)
                                                              cATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACg                                                                          >  1:1458224/1‑140 (MQ=255)
                                                                 cGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGttt                                                                       <  1:4205308/140‑1 (MQ=255)
                                                                    cATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCg                                                                    <  2:4384462/140‑1 (MQ=255)
                                                                      tCGCCTTTAACAATGCGTTGTAACCTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCGGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGa                                                                  <  2:1345651/140‑1 (MQ=255)
                                                                      tCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGa                                                                  >  1:107441/1‑140 (MQ=255)
                                                                                 aaTGCTTTGTAAACTCTCGTGATAAGACAGATCGACTCGTTCCACCTCACCACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTAc                                                       <  2:3387014/140‑1 (MQ=255)
                                                                                   tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACgg                                                     >  1:712258/1‑140 (MQ=255)
                                                                                   tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACgg                                                     >  1:1225376/1‑140 (MQ=255)
                                                                                   tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACgg                                                     >  2:4138198/1‑140 (MQ=255)
                                                                                   tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACgg                                                     >  1:2216475/1‑140 (MQ=255)
                                                                                   tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACgg                                                     >  2:4983223/1‑140 (MQ=255)
                                                                                    gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                                    >  1:650628/1‑140 (MQ=255)
                                                                                    gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                                    >  2:4618135/1‑140 (MQ=255)
                                                                                    gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                                    >  1:1352953/1‑140 (MQ=255)
                                                                                    gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                                    >  1:5042973/1‑140 (MQ=255)
                                                                                       ttGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCaaa                                                 <  2:3016143/140‑1 (MQ=255)
                                                                                            aaCTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAAc                                            <  1:3556034/140‑1 (MQ=255)
                                                                                            aaCTCTCGTGATAAGAGAGATCGACTCGTTACACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCGCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAAc                                            <  2:1577230/140‑1 (MQ=255)
                                                                                               tctcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGCTTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCCCGTTTGCGGACTCGCCTTTACGGCAAATCAACTgg                                         >  1:4815914/1‑140 (MQ=255)
                                                                                               tctcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTgg                                         >  2:1248991/1‑140 (MQ=255)
                                                                                                 tcGTGATAAGGGAGCTTGACTCGTTCCACATCACTACTGCC‑AAAATAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCACACGCGCTTCACGATTACGGACTCGGCTTTACGGAAAATCAACTGGTg                                       >  2:2920067/1‑140 (MQ=255)
                                                                                                 tcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg                                       >  1:1843675/1‑140 (MQ=255)
                                                                                                  cGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg                                       <  2:1079524/140‑1 (MQ=255)
                                                                                                  cGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGc                                      >  1:3944310/1‑140 (MQ=255)
                                                                                                    tGATCAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc                                    <  1:1518428/140‑1 (MQ=255)
                                                                                                    tGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGt                                                                                                                           >  1:1970665/1‑53 (MQ=255)
                                                                                                    tGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGt                                                                                                                           <  2:1970665/53‑1 (MQ=255)
                                                                                                    tGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc                                    <  1:1282112/140‑1 (MQ=255)
                                                                                                    ggATAAGAGAGATCGACTCGTTCCACATCATTACCGAC‑AAACAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACCCGCTTCACGTTTGCGGACTCGCCTTTACGGAAAATCAACTGGTGCTc                                    <  2:673786/139‑1 (MQ=255)
                                                                                                      aTAAGAGTGATCACCTCGTTCCACATCA‑GAGCCTCTAAAAAAATCGTCGGTCACGATTTTGCCATCTGCCAAACGGTTATCCAGACCACCGCGCTTCACGTGTGCGGACTCGATTTTACGGCAAATCAACTGGTGCTCGc                                  <  2:4445652/140‑1 (MQ=255)
                                                                                                      aTAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑CAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                                  <  2:3770526/140‑1 (MQ=255)
                                                                                                      aTAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                                  <  1:375062/140‑1 (MQ=255)
                                                                                                      aTAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                                  <  1:1609392/140‑1 (MQ=255)
                                                                                                      aTAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                                  <  1:2533210/140‑1 (MQ=255)
                                                                                                       tAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGcc                                 <  2:2217109/140‑1 (MQ=255)
                                                                                                         agagagATCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCaa                               >  1:2556482/1‑140 (MQ=255)
                                                                                                           agagaTCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACg                             >  1:2364812/1‑140 (MQ=255)
                                                                                                           agagaTCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACg                             >  1:1000219/1‑140 (MQ=255)
                                                                                                               aTCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGTACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAgg                         >  2:5061643/1‑140 (MQ=255)
                                                                                                               aTCGACTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAgg                         >  1:3357320/1‑140 (MQ=255)
                                                                                                               aTCGACTCGTTCCACATCACTACAGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCCAATCAACTGGTGCTCGCCAACGTAgg                         >  1:951685/1‑140 (MQ=255)
                                                                                                                   aCTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGtgtg                     <  2:4780356/140‑1 (MQ=255)
                                                                                                                    cTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGtgtgg                    >  1:4512979/1‑139 (MQ=255)
                                                                                                                    cTCGTTCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGc                    <  1:3086496/140‑1 (MQ=255)
                                                                                                                       gTTCCACATCACTACCG‑CGAAAAAAAGCATGGGTCATGATTTTCTGATCCCCCGTACGGTTATCCAGCCCCACGCCCTTCACGTTTGCGGACTCGCCTTTACGGCAGCGCAACTGGTGCTCGCCAACGTCGGCGTGGGGc                 >  2:2056789/1‑140 (MQ=255)
                                                                                                                        ttCCACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGcc                >  1:3828884/1‑140 (MQ=255)
                                                                                                                          ccACATCACTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGCCTCGCCTTTACGGCAAATCAACTGGTGATCGCCAACGTAGGTGTGCGGcccc              <  2:402461/140‑1 (MQ=255)
                                                                                                                                  cTACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAg      >  2:3678939/1‑140 (MQ=255)
                                                                                                                                  cTACCGCC‑AAAAAAAACATCGGTCAGGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCGTCACGTTTGCGGAGTGGCCGTTACGGCAAATCAACTGGGGCTCGCCAACGTAGGTGTTCGGCCCCGACTCCAg      >  2:2784009/1‑140 (MQ=255)
                                                                                                                                   tACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGc                 <  1:3595107/128‑1 (MQ=255)
                                                                                                                                   tACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAgc     >  1:3097098/1‑140 (MQ=255)
                                                                                                                                   tACCGCC‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAgc     >  1:2271489/1‑140 (MQ=255)
                                                                                                                                       cgcAATAACAAACGTCGGTCATGATTTTCTGGTCCGTCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGACCCAACTCCAGcgcg  <  2:2597352/138‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                        
CGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAGCGCG  >  NZ_CP025268/3512286‑3512560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑