BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	674,649	+T	pseudogene (947/977 nt)	ybeQ ←	SEL1‑like repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	674,649	1	.	T	99.3%	1155.2 / ‑1.7	305	pseudogene (947/977 nt)	ybeQ	SEL1‑like repeat protein
Reads supporting (aligned to +/- strand): ref base . (2/0); new base T (136/167); total (138/167)
Fisher's exact test for biased strand distribution p-value = 2.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 313 total aligned reads displayed.

TGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAGTGGCGTC  >  NZ_CP025268/674510‑674773

  gTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAACTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTcctg                                                                                                                             >  2:4565594/1‑138 (MQ=255)

     aTGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTcctgact                                                                                                                          <  1:3448272/140‑6 (MQ=255)

                cAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTgg                                                                                                               <  1:4900622/140‑1 (MQ=255)

                       gCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGa                                                                                                        >  2:1216268/1‑140 (MQ=255)

                       gCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGa                                                                                                        >  2:3530621/1‑140 (MQ=255)

                       gCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGa                                                                                                        >  2:2311381/1‑140 (MQ=255)

                       gCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGa                                                                                                        >  1:3983303/1‑140 (MQ=255)

                       gCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGa                                                                                                        >  1:4259804/1‑140 (MQ=255)

                       gCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGa                                                                                                        >  2:1214191/1‑140 (MQ=255)

                       gCCCGTTTGAGCCGTAATAGTCGGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGa                                                                                                        >  1:3365497/1‑140 (MQ=255)

                          cGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGa                                                                                                     <  2:1766510/140‑1 (MQ=255)

                            tttGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACt                                                                                                   <  2:799014/140‑1 (MQ=255)

                               gAGCCGTAATAGTCTGATTTCGCACAGCCATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGaa                                                                                                <  1:4733704/140‑1 (MQ=255)

                               gAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGaa                                                                                                <  1:769053/140‑1 (MQ=255)

                               gAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGaa                                                                                                <  1:991644/140‑1 (MQ=255)

                               gAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGaa                                                                                                <  2:1382920/140‑1 (MQ=255)

                               gAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGaa                                                                                                <  2:4903541/140‑1 (MQ=255)

                               gAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGaa                                                                                                <  1:2376810/140‑1 (MQ=255)

                               gAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGaa                                                                                                <  1:183206/140‑1 (MQ=255)

                               gAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGaa                                                                                                <  1:1168646/140‑1 (MQ=255)

                               gAGCCGTAATAGTATGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGaa                                                                                                <  1:545855/140‑1 (MQ=255)

                                 gCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACt                                                                                              >  1:1926050/1‑140 (MQ=255)

                                 gCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCCGATGACTGAACt                                                                                              >  2:1369555/1‑140 (MQ=255)

                                    gTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTa                                                                                           <  2:11479/140‑1 (MQ=255)

                                    gTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTa                                                                                           <  2:2851490/140‑1 (MQ=255)

                                     tAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTAc                                                                                          >  1:4554067/1‑140 (MQ=255)

                                       aTAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGa                                                                                        >  2:2388729/1‑140 (MQ=255)

                                       aTAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGa                                                                                        <  1:3948050/140‑1 (MQ=255)

                                       aTAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTATCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGa                                                                                        <  1:4214056/140‑1 (MQ=255)

                                         aGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATa                                                                                      <  2:2908551/140‑1 (MQ=255)

                                         aGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATa                                                                                      <  1:4839041/140‑1 (MQ=255)

                                          gTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATT‑CCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACa                                                                                    <  1:3544817/140‑1 (MQ=255)

                                           tCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACa                                                                                    >  1:1210435/1‑140 (MQ=255)

                                            cTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACAtctc                                                                                                                  <  1:1724828/109‑1 (MQ=255)

                                               aTTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTc                                                                                >  2:2264354/1‑140 (MQ=255)

                                               aTTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTc                                                                                >  2:4052504/1‑140 (MQ=255)

                                                 ttCGCACAGCACTAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCga                                                                              <  2:662278/140‑1 (MQ=255)

                                                 ttCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCga                                                                              <  1:3761718/140‑1 (MQ=255)

                                                  tCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCgag                                                                             <  1:1624331/140‑1 (MQ=255)

                                                     cacaGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGc                                                                          >  2:4688030/1‑140 (MQ=255)

                                                         gCAATACAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGt                                                                      <  2:4199103/140‑1 (MQ=255)

                                                         gCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGt                                                                      >  2:5083327/1‑140 (MQ=255)

                                                             tAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAAGTTCCTAACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGCTCGAGAGCCTGTCGga                                                                  >  1:2845684/1‑140 (MQ=255)

                                                                 gagaATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATc                                                              <  2:1532948/140‑1 (MQ=255)

                                                                   gaATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGa                                                            <  2:828498/140‑1 (MQ=255)

                                                                    aaTATGGGCCGAATGAAATTCCCCGGCTCGTGTTTTTATCTCTACGGTAAATCGTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGAGGACTGAACTTTACGATACAGGTCGAGAGACTGTGGGAGATCGAc                                                           >  1:3713186/1‑140 (MQ=255)

                                                                    aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGGTGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGAc                                                           >  2:2650244/1‑140 (MQ=255)

                                                                    aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGAc                                                           >  1:3848831/1‑140 (MQ=255)

                                                                    aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGAc                                                           >  2:4620692/1‑140 (MQ=255)

                                                                    aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGAc                                                           >  1:2105162/1‑140 (MQ=255)

                                                                    aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGAc                                                           >  1:300070/1‑140 (MQ=255)

                                                                    aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGAc                                                           >  1:1394298/1‑140 (MQ=255)

                                                                    aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGAc                                                           >  1:703843/1‑140 (MQ=255)

                                                                    aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGAc                                                           >  1:3219352/1‑140 (MQ=255)

                                                                    aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGAc                                                           >  1:2002978/1‑140 (MQ=255)

                                                                    aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGAc                                                           >  1:5075864/1‑140 (MQ=255)

                                                                    aaTATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGAc                                                           >  1:1585577/1‑140 (MQ=255)

                                                                     atatGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACa                                                          <  2:2114092/140‑1 (MQ=255)

                                                                     atatGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACa                                                          <  1:1001175/140‑1 (MQ=255)

                                                                     atatGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACa                                                          <  1:2957234/140‑1 (MQ=255)

                                                                      tatGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAg                                                         <  2:3428567/140‑1 (MQ=255)

                                                                       atGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAgg                                                        <  1:5111739/140‑1 (MQ=255)

                                                                       atGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAgg                                                        <  1:2816345/140‑1 (MQ=255)

                                                                        tGGGCCGAATGAACTTCCCCGGCCCTTGTTTTTATCTCTACAGTAGATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTTCGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGt                                                       <  2:4418748/140‑1 (MQ=255)

                                                                        tGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAGATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGt                                                       <  1:2737843/140‑1 (MQ=255)

                                                                          ggCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTct                                                     <  1:494225/140‑1 (MQ=255)

                                                                           gCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTctt                                                    >  1:207069/1‑140 (MQ=255)

                                                                            ccGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTcttc                                                   <  2:3823010/140‑1 (MQ=255)

                                                                            ccGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTcttc                                                   <  2:2133522/140‑1 (MQ=255)

                                                                            ccGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTcttc                                                   <  2:4259804/140‑1 (MQ=255)

                                                                            ccGAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTcttc                                                   >  2:2942964/1‑140 (MQ=255)

                                                                              gAATGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTcttcct                                                 >  1:4698383/1‑140 (MQ=255)

                                                                               aaTGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTcttcctt                                                <  1:4435432/140‑1 (MQ=255)

                                                                               aaTGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTcttcctt                                                <  2:2758710/140‑1 (MQ=255)

                                                                                aTGAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTcttccttc                                               >  2:1991895/1‑140 (MQ=255)

                                                                                  gAAATTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTtctc                                             <  1:865986/140‑1 (MQ=255)

                                                                                    aaTTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCg                                           <  2:874896/140‑1 (MQ=255)

                                                                                    aaTTCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCg                                           <  1:3139784/140‑1 (MQ=255)

                                                                                       tCCCCGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAg                                        <  2:2107427/140‑1 (MQ=255)

                                                                                           cGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTcttc                                                   <  2:3070557/125‑1 (MQ=255)

                                                                                           cGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGAt                                    <  1:2047347/140‑1 (MQ=255)

                                                                                           cGGCCCTTGTTTTTATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGAt                                    <  1:2433021/140‑1 (MQ=255)

                                                                                           cGGCCCTTGTTTTTATCTCTACAGTAAATCGTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTcttc                                                   >  1:3070557/1‑125 (MQ=255)

                                                                                                    tttttATCTCTACAGTAAATCTTCAGTCTCACGAATTTCGTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATgg                           <  2:2421896/140‑1 (MQ=255)

                                                                                                     ttttATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGc                          <  1:1111754/140‑1 (MQ=255)

                                                                                                     ttttATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGc                          <  2:3337972/140‑1 (MQ=255)

                                                                                                     ttttATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGc                          <  1:4155038/140‑1 (MQ=255)

                                                                                                     ttttATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGc                          <  2:1702141/140‑1 (MQ=255)

                                                                                                     ttttATCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGc                          <  2:3862027/140‑1 (MQ=255)

                                                                                                         aTCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAgg                      <  1:2241447/140‑1 (MQ=255)

                                                                                                         aTCTCTACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAgg                      <  2:3563620/140‑1 (MQ=255)

                                                                                                             ctACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTtat                  >  1:1516549/2‑140 (MQ=255)

                                                                                                              tACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCg                                                                                                          >  1:1903707/1‑51 (MQ=255)

                                                                                                              tACAGTAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTtata                 <  2:411156/140‑1 (MQ=255)

                                                                                                                   tAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAgg            <  2:3941224/140‑1 (MQ=255)

                                                                                                                   tAAATCTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAgg            >  1:2520759/1‑140 (MQ=255)

                                                                                                                        cTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAGTg       >  1:2633678/1‑140 (MQ=255)

                                                                                                                        cTTCAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAGTg       >  2:3792188/1‑140 (MQ=255)

                                                                                                                           cAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAGTGGCg    <  2:1912908/140‑1 (MQ=255)

                                                                                                                           cAGTCTCACGAATTTCCTGACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAGTGGCg    <  2:3622346/140‑1 (MQ=255)

TGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGTTTTTATCTTTACAGTAAATCTTCAGTCTCACGAATTTCC‑GACTGACATCTCTGGTTCCGGATGACTGAACTTTACGATACAGGTCGAGAGCCTGTCGGAGATCGACAGGTCTTCCTTCTCCGTAGTGATACATAATGGCCAGGTTATACCAGGCAGTGGCGTC  >  NZ_CP025268/674510‑674773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`