BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,224,095	+GA	coding (248/249 nt)	CXP41_RS06440 →	protein YmgC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,224,094	1	.	A	99.1%	823.9 / ‑0.8	215	coding (247/249 nt)	CXP41_RS06440	protein YmgC
Reads supporting (aligned to +/- strand): ref base . (1/1); new base A (123/90); total (124/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01
*	NZ_CP025268	1,224,094	2	.	G	99.1%	720.2 / ‑0.5	215	coding (247/249 nt)	CXP41_RS06440	protein YmgC
Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (123/89); total (124/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 235 total aligned reads displayed.

CAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑A‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTC  >  NZ_CP025268/1223956‑1224223

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cAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑a                                                                                                                                    <  2:2967500/140‑1 (MQ=255)

cAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑a                                                                                                                                    >  1:2121083/1‑140 (MQ=255)

 aGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑ag                                                                                                                                   >  2:1383264/1‑139 (MQ=255)

 aGGATTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATAACATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑ag                                                                                                                                   >  2:5194/1‑139 (MQ=255)

     tttACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑agaact                                                                                                                               >  1:3337113/1‑135 (MQ=255)

           cTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTa                                                                                                                         <  1:5035344/140‑1 (MQ=255)

           cTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTa                                                                                                                         <  2:3337113/140‑1 (MQ=255)

             tGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGCTATCATTATTAATGACAGGGTATCCGTGCTCTCTTAGA‑A‑CTTGCGTAAg                                                                                                                       >  2:2070976/1‑140 (MQ=255)

             tGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAg                                                                                                                       >  2:4087934/1‑140 (MQ=255)

             tGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAg                                                                                                                       >  1:836158/1‑140 (MQ=255)

             tGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAg                                                                                                                       >  2:3064975/1‑140 (MQ=255)

             tGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAg                                                                                                                       >  1:3950851/1‑140 (MQ=255)

               cGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGt                                                                                                                     >  1:1807241/1‑140 (MQ=255)

               cGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑agaac                                                                                                                                <  2:3099018/129‑5 (MQ=255)

                 tttAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGttt                                                                                                                   >  2:3167348/1‑140 (MQ=255)

                 tttAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGttt                                                                                                                   >  2:2806784/1‑140 (MQ=255)

                 tttAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGttt                                                                                                                   <  2:2526700/140‑1 (MQ=255)

                              aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACacc                                                                                                      <  1:4089245/140‑3 (MQ=255)

                              aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTAAATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTGAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATc                                                                                                      <  1:4522891/140‑1 (MQ=255)

                                cAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAg                                                                                                    <  1:1005000/140‑1 (MQ=255)

                                cAGTGTGATTCTTTCTGATATCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCCTTATTAATGACAGGGCATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAg                                                                                                    <  1:1098675/140‑1 (MQ=255)

                                cAGAGTGATTCTTTATGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTAAACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAg                                                                                                    <  1:4138615/140‑1 (MQ=255)

                                   tgtgATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGtttt                                                                                                                  >  1:3877428/1‑123 (MQ=255)

                                   tgtgATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTg                                                                                                 <  2:32547/140‑1 (MQ=255)

                                   tgtgATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTg                                                                                                 >  1:1528256/1‑140 (MQ=255)

                                       aTTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATc                                                                                             >  2:4024858/1‑140 (MQ=255)

                                        ttctttctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCa                                                                                            >  1:2107114/1‑140 (MQ=255)

                                             tctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATg                                                                                       <  1:1489155/140‑1 (MQ=255)

                                             gctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATg                                                                                       >  2:2036658/2‑140 (MQ=255)

                                               tGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCt                                                                                     >  2:933307/1‑140 (MQ=255)

                                               tGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCt                                                                                     >  1:2949756/1‑140 (MQ=255)

                                               tGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCt                                                                                     <  1:2408047/140‑1 (MQ=255)

                                                 aTCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATAGCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CGTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGa                                                                                   >  2:1195821/1‑140 (MQ=255)

                                                     ctATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCa                                                                               <  2:3570366/140‑1 (MQ=255)

                                                        tCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAg                                                                            >  1:486377/1‑140 (MQ=255)

                                                            cAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                        <  2:732331/140‑1 (MQ=255)

                                                            cAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                        <  2:4762008/140‑1 (MQ=255)

                                                            cAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                        <  1:4242789/140‑1 (MQ=255)

                                                              aaTATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTACGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCa                                                                      >  2:4631789/1‑140 (MQ=255)

                                                                tatTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATg                                                                                       <  2:2354871/121‑1 (MQ=255)

                                                                tatTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATg                                                                                       >  1:2354871/1‑121 (MQ=255)

                                                                  ttGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTc                                                                  <  1:3674669/140‑1 (MQ=255)

                                                                         aCATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGtt                                                           <  1:3490766/140‑1 (MQ=255)

                                                                            ttGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                        <  1:4781510/140‑1 (MQ=255)

                                                                            ttGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                        <  2:1815297/140‑1 (MQ=255)

                                                                            ttGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                        <  1:697975/140‑1 (MQ=255)

                                                                            ttGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                        <  1:3090921/140‑1 (MQ=255)

                                                                              gAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCt                                                      <  1:667406/140‑1 (MQ=255)

                                                                              gAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCt                                                      <  1:3253655/140‑1 (MQ=255)

                                                                               aaaTGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCtt                                                     >  2:3295260/1‑140 (MQ=255)

                                                                                  tGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCt                                                                                     >  2:2761797/1‑105 (MQ=255)

                                                                                  tGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCt                                                  >  2:4110268/1‑140 (MQ=255)

                                                                                   gCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCGTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAAGATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCtt                                                 <  1:979878/140‑1 (MQ=255)

                                                                                    cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTa                                                                                                                 <  2:1707725/75‑1 (MQ=255)

                                                                                    cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTa                                                                                                                 >  1:1707725/1‑75 (MQ=255)

                                                                                    cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                        <  1:4185709/132‑1 (MQ=255)

                                                                                    cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                        >  2:4185709/1‑132 (MQ=255)

                                                                                    cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCtta                                                >  2:1261120/1‑140 (MQ=255)

                                                                                      tAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCttatt                                              <  1:2864559/140‑1 (MQ=255)

                                                                                       aaGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGttt                                                                                                                   <  1:2504717/70‑1 (MQ=255)

                                                                                       aaGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGttt                                                                                                                   >  2:2504717/1‑70 (MQ=255)

                                                                                        aGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTtattat                                            <  1:2806764/140‑1 (MQ=255)

                                                                                         gACTACACTAACGGCTATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGATTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATg                                           >  2:4094779/1‑140 (MQ=255)

                                                                                         gACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTc                                                                  <  2:1978681/117‑1 (MQ=255)

                                                                                         gACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTc                                                                  >  1:1978681/1‑117 (MQ=255)

                                                                                         gACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATg                                           >  2:2140008/1‑140 (MQ=255)

                                                                                            tACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTcc                                        >  1:1845415/1‑140 (MQ=255)

                                                                                            tACACTAACGGATATCATTATTAATGACAGGGAATCCGTGATCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGATTTCTTATTATGTcc                                        <  1:3111575/140‑1 (MQ=255)

                                                                                              cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGc                                      >  1:3642906/1‑140 (MQ=255)

                                                                                              cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGc                                      >  1:3805549/1‑140 (MQ=255)

                                                                                              cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGc                                      >  1:869174/1‑140 (MQ=255)

                                                                                              cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGc                                      >  1:4583960/1‑140 (MQ=255)

                                                                                              cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGc                                      <  2:3947157/140‑1 (MQ=255)

                                                                                              cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGc                                      >  1:5094330/1‑140 (MQ=255)

                                                                                              cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGc                                      >  1:4991513/1‑140 (MQ=255)

                                                                                              cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGc                                      >  1:1981731/1‑140 (MQ=255)

                                                                                               acTAACGGATATCATTATTAATGACAGGGAATCTGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCa                                     <  2:2821575/140‑1 (MQ=255)

                                                                                               acTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCa                                     <  2:178389/140‑1 (MQ=255)

                                                                                                   aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTCTGCTTTCTTATTATGTCCGCAatat                                 >  1:4652176/1‑140 (MQ=255)

                                                                                                   aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAatat                                 >  2:4964301/1‑140 (MQ=255)

                                                                                                   aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAatat                                 >  1:934316/1‑140 (MQ=255)

                                                                                                   aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AGAGCTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAatat                                 >  2:4472387/1‑140 (MQ=255)

                                                                                                    cGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATc                                >  1:2561921/1‑140 (MQ=255)

                                                                                                      gATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAg                              >  2:2312831/1‑140 (MQ=255)

                                                                                                        tatCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAgtt                            >  2:143707/1‑138 (MQ=255)

                                                                                                        tatCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGgt                            <  1:1922427/140‑1 (MQ=255)

                                                                                                        tatCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGgt                            <  2:3737/140‑1 (MQ=255)

                                                                                                        tatCATTATTAATGACAGGGAATCCGTGCTCTCGTAGA‑A‑CTTGAGTAAGGTTGTAGAATAAACATCAGCTGTAGCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTGTTCTTTGTTATTAGGTCGGCAATATCAGgt                            >  2:652978/1‑140 (MQ=255)

                                                                                                         atCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGgtg                           >  1:2990646/1‑140 (MQ=255)

                                                                                                         atCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGgtg                           >  1:549778/1‑140 (MQ=255)

                                                                                                         atCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGgtg                           >  1:4922286/1‑140 (MQ=255)

                                                                                                                 taATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAAt                   >  2:4942929/1‑140 (MQ=255)

                                                                                                                     gACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGgag               >  1:1430749/1‑140 (MQ=255)

                                                                                                                        aGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGt            >  1:3393702/1‑140 (MQ=255)

                                                                                                                         gggAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTAGTATGTCCGCAATATCATGTGTCAAGAATGGAGAGtt           >  2:553004/1‑140 (MQ=255)

                                                                                                                          ggAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTtc          >  1:3123328/1‑140 (MQ=255)

                                                                                                                          ggAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTtc          >  2:4980296/1‑140 (MQ=255)

                                                                                                                          ggAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTtc          >  1:1633889/1‑140 (MQ=255)

                                                                                                                          ggAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTCATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTtc          >  1:4045436/1‑140 (MQ=255)

                                                                                                                                cGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGctc    <  1:2745823/140‑1 (MQ=255)

||

CAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑A‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTC  >  NZ_CP025268/1223956‑1224223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`