BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,374,862	(T)_8→9	intergenic (+40/‑172)	pspE → / → ycjM	thiosulfate sulfurtransferase PspE/glucosylglycerate phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,374,854	1	.	T	94.9%	991.6 / 36.8	276	intergenic (+32/‑180)	pspE/ycjM	thiosulfate sulfurtransferase PspE/glucosylglycerate phosphorylase
Reads supporting (aligned to +/- strand): ref base . (3/11); new base T (132/129); total (136/140)
Fisher's exact test for biased strand distribution p-value = 5.18e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 299 total aligned reads displayed.

GGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑G‑‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCG  >  NZ_CP025268/1374715‑1374989

ggACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑g                                                                                                                                            >  2:2179489/1‑140 (MQ=255)

 gACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑G‑‑t                                                                                                                                         >  2:4375999/1‑140 (MQ=255)

   cGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCTGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCTTGATCGGCGCTTATGCGCC‑G‑‑ttt                                                                                                                                       >  2:2377015/1‑140 (MQ=255)

        gTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑G‑‑tttttttt                                                                                                                                  >  1:3036477/1‑140 (MQ=255)

         taaGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATACCGAAAGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑G‑‑ttttttttt                                                                                                                                 <  2:3049645/138‑2 (MQ=255)

         tCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑G‑‑ttttttttt                                                                                                                                 <  2:1844398/140‑2 (MQ=255)

         tCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑G‑‑ttttttttt                                                                                                                                 <  2:534992/140‑2 (MQ=255)

          cAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑G‑‑tttttttttt                                                                                                                                <  2:4777006/140‑3 (MQ=255)

            gggCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCAGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTCAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑G‑‑tttttttttctg                                                                                                                              >  2:2597736/1‑136 (MQ=255)

             ggCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑G‑‑tttttttttctgt                                                                                                                             <  1:1467342/140‑6 (MQ=255)

             ggCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑G‑‑tttttttttctgt                                                                                                                             <  2:2129719/140‑6 (MQ=255)

               caagcaaAAGAGATCCTTAGCGAGGTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATTGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCCCCATGAGCGGCGCTTATGCGCCGG‑‑TTTTTTTT‑CTGTca                                                                                                                           <  2:594589/140‑1 (MQ=255)

               caagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTca                                                                                                                           <  1:2411009/140‑1 (MQ=255)

               aaagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATGCACCATGAGCTGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTca                                                                                                                           <  2:3709650/139‑1 (MQ=255)

                aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTcac                                                                                                                          <  1:750819/140‑1 (MQ=255)

                aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTcac                                                                                                                          <  2:1383260/140‑1 (MQ=255)

                aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTcac                                                                                                                          <  1:3985232/140‑1 (MQ=255)

                aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGGTGAGAACGCCGGTGGCCTGAAAGAGATCGCCATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTcac                                                                                                                          <  2:3898715/140‑1 (MQ=255)

                 agcaaCAGAGAGCCTTACCGAGATGGGATATACCCACGTTGAGAACGCCGGTGTCCTGAAAGACATCGCAATGCCGAAGGTCAAATGTTAAAAGCATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTcaca                                                                                                                         <  2:3222774/140‑1 (MQ=255)

                   caaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACAcc                                                                                                                       <  1:1337494/140‑1 (MQ=255)

                   caaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACAcc                                                                                                                       <  1:1649429/140‑1 (MQ=255)

                       agagATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTAt                                                                                                                   >  1:4513769/1‑140 (MQ=255)

                       agagATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTAt                                                                                                                   >  1:2500661/1‑140 (MQ=255)

                       agagATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTAt                                                                                                                   >  1:3864323/1‑140 (MQ=255)

                          gaTCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTa                                                                                                                <  1:498737/140‑1 (MQ=255)

                          gaTCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTa                                                                                                                <  1:1859966/140‑1 (MQ=255)

                            tCCTTAGCGAGATGGGCTATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACa                                                                                                              <  1:4164154/140‑1 (MQ=255)

                            tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACa                                                                                                              <  1:4893635/140‑1 (MQ=255)

                            tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACa                                                                                                              <  2:1606106/140‑1 (MQ=255)

                            tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACa                                                                                                              <  2:1577690/140‑1 (MQ=255)

                             ccTTAGCTAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACAt                                                                                                             <  2:1496668/140‑1 (MQ=255)

                             ccTTAGAGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGCTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGG‑‑TGTTTTTT‑CTGTCACACCTTATTTACAt                                                                                                             <  1:4965531/140‑1 (MQ=255)

                                tAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATTGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATccc                                                                                                          >  1:3074037/1‑140 (MQ=255)

                                 aGCGAGATGGGGTATACCCACGTTGAGACCGCCGGTGGCCTGAAAGACATCGCAATGCCGAATGTCAAAGGTTAAAAGAATTCACCATGAGCGTCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATcccc                                                                                                         <  2:4505474/140‑1 (MQ=255)

                                   cGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCAt                                                                                                       >  1:647147/1‑140 (MQ=255)

                                     agaTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAg                                                                                                     >  1:171496/1‑140 (MQ=255)

                                       aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GTTTTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGa                                                                                                    >  1:1773981/1‑140 (MQ=255)

                                       aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGat                                                                                                   >  1:22609/1‑140 (MQ=255)

                                       aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGat                                                                                                   >  2:1660396/1‑140 (MQ=255)

                                       aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGat                                                                                                   >  1:3369441/1‑140 (MQ=255)

                                       aTGGGATATACCCACGTTAAGAACGCCGGTAGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGat                                                                                                   >  2:5031074/1‑140 (MQ=255)

                                        tGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGatt                                                                                                  >  1:3723188/1‑140 (MQ=255)

                                        tGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGa                                                                                                    >  1:2222247/1‑138 (MQ=255)

                                                aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                          <  1:5121088/140‑1 (MQ=255)

                                                aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                          <  1:1080356/140‑1 (MQ=255)

                                                aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                          <  1:331/140‑1 (MQ=255)

                                                aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                          >  1:1915135/1‑140 (MQ=255)

                                                  ccACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCa                                                                                        >  2:2768771/1‑140 (MQ=255)

                                                    aCGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTGAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGACTATTTGCGTCAGc                                                                                      >  2:4431754/1‑140 (MQ=255)

                                                    aCGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGc                                                                                      >  1:1633231/1‑140 (MQ=255)

                                                     cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGCAGGTCAAAGGTTACAAGCATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGcg                                                                                     >  2:4745304/1‑139 (MQ=255)

                                                     cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCATCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                     >  2:3653525/1‑140 (MQ=255)

                                                     cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                     >  2:3857880/1‑140 (MQ=255)

                                                     cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                     >  2:2582463/1‑140 (MQ=255)

                                                     cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                     >  1:2364980/1‑140 (MQ=255)

                                                     cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                     >  2:4930982/1‑140 (MQ=255)

                                                     cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                     >  2:4811504/1‑140 (MQ=255)

                                                     cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                     <  1:2893427/140‑1 (MQ=255)

                                                     cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                     >  2:2039858/1‑140 (MQ=255)

                                                     cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                     >  1:377640/1‑140 (MQ=255)

                                                     cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                     >  2:3468111/1‑140 (MQ=255)

                                                     cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                     >  2:4389438/1‑140 (MQ=255)

                                                     cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                     >  1:436264/1‑140 (MQ=255)

                                                     cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                     >  1:4427290/1‑140 (MQ=255)

                                                          agaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTCAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTGACACCTTATTTACATCGCCATAGATTATTTGCGTGAGCTCaaaa                                                                                >  2:3520027/1‑140 (MQ=255)

                                                          agaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTAt                                                                                                                   >  2:838500/1‑105 (MQ=255)

                                                          agaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACaa                                                                                >  1:3560424/1‑140 (MQ=255)

                                                            aaCGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAAt                                                                              >  1:3192806/1‑140 (MQ=255)

                                                                ccGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGACACACCTTATTTACATCCCCATGGATTATTTGCGTTTGCTCACAAATATGc                                                                          >  2:1740386/1‑140 (MQ=255)

                                                                 cGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCt                                                                         <  1:814638/140‑1 (MQ=255)

                                                                       ccTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTcc                                                                   <  1:2121320/140‑1 (MQ=255)

                                                                           aaaGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GTTTTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTg                                                                >  1:189846/1‑140 (MQ=255)

                                                                            aaGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGt                                                              <  2:4580876/140‑1 (MQ=255)

                                                                                 aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGAC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaaa                                                         >  2:179494/1‑140 (MQ=255)

                                                                                  tCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaaaa                                                        >  2:1231650/1‑140 (MQ=255)

                                                                                              aGGTGAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCg                                            <  1:2462649/140‑1 (MQ=255)

                                                                                              aGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCg                                            <  2:4346131/140‑1 (MQ=255)

                                                                                              aGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCg                                            <  2:4226112/140‑1 (MQ=255)

                                                                                              aGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCg                                            <  2:758341/140‑1 (MQ=255)

                                                                                               ggTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGGCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGt                                           <  2:4013062/140‑1 (MQ=255)

                                                                                                    aaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTa                                      >  2:2689150/1‑140 (MQ=255)

                                                                                                    aaGGTTAAAAGAATTCACCATGAGCGGCGCTGATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACCTCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTa                                      >  2:2958954/1‑140 (MQ=255)

                                                                                                          aaaaGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAAccc                                >  1:1107319/1‑140 (MQ=255)

                                                                                                          aaaaGAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAAccc                                >  2:2206627/1‑140 (MQ=255)

                                                                                                              gAATTCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTtgt                            <  1:2186101/140‑1 (MQ=255)

                                                                                                              gAACTCACTCTGATCGGCGCTTATACGCC‑GT‑TTTTTTTT‑CTGTCCCACCTTCTTTACATCCCCATAGATTATTTGCGTCAGCTCACAAAGACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTtgt                            <  2:2265684/140‑1 (MQ=255)

                                                                                                                 ttCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTACAACCCTTGTGCt                         >  2:483836/1‑140 (MQ=255)

                                                                                                                 ttCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCt                         >  2:637943/1‑140 (MQ=255)

                                                                                                                 ttCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCt                         >  2:1295898/1‑140 (MQ=255)

                                                                                                                 ttCACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCt                         >  1:314108/1‑140 (MQ=255)

                                                                                                                   cACCATGAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCa                       >  1:3954876/1‑140 (MQ=255)

                                                                                                                         gAGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGAc                 <  1:4105829/140‑1 (MQ=255)

                                                                                                                          aGCGGCGCTTATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTTTGCTCAATTGACa                >  2:2659619/1‑140 (MQ=255)

                                                                                                                                   tATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAtttt       <  1:2631744/140‑1 (MQ=255)

                                                                                                                                   tATGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAtttt       <  1:983505/140‑1 (MQ=255)

                                                                                                                                    aTGCGGC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACAGCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCGATTGACAGTTTAttttt      >  2:4523588/1‑139 (MQ=255)

                                                                                                                                    aTGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCGCAAATACGCGTTTTCCCTGGTAAAAAATGATTTTCTGCGTGACAAAAACCCTTGTGCTCAATTGATAGTTTATTTTc      >  2:3002464/1‑140 (MQ=255)

                                                                                                                                    aTGCGCC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTc      >  2:3818291/1‑140 (MQ=255)

                                                                                                                                      gcgcC‑GT‑TTTTTTTT‑CTGTCACACCTTATTTACAGCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTg    <  1:908783/140‑1 (MQ=255)

                                                                                                                                        gcC‑G‑‑TTTTTTTTCCTGTCACACCTTATTTCCATCCCCATCGATTATTTGCGTCAGCTCACAACTACGCGTTTTCCCGGGTACAAAATGATTGCCTGCGTGACTACAACCCGTGTGCTCAATTGACAGTTTATTGTCTGCg  <  2:3416330/140‑1 (MQ=255)

GGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCC‑G‑‑TTTTTTTT‑CTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCG  >  NZ_CP025268/1374715‑1374989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`