BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,855,625	+C	intergenic (+116/‑50)	sppA → / → ansA	signal peptide peptidase SppA/asparaginase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,855,621	1	.	C	96.6%	955.6 / 22.5	293	intergenic (+112/‑54)	sppA/ansA	signal peptide peptidase SppA/asparaginase
Reads supporting (aligned to +/- strand): ref base . (2/8); new base C (99/183); total (101/192)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 312 total aligned reads displayed.

TGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑T‑C‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGGTG  >  NZ_CP025268/1855483‑1855755

tGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑T‑c                                                                                                                                         >  2:3339711/1‑140 (MQ=255)

tGCCTGACCTGCGCCAACATGCGTTAAGTATTGTACTGAGTGGCCGACAGATCGGCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑T‑c                                                                                                                                         >  2:3301573/1‑140 (MQ=255)

  ccTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑T‑c‑c‑c                                                                                                                                     <  1:267181/140‑1 (MQ=255)

   cTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGCCAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑T‑c‑c‑cc                                                                                                                                    <  1:2546135/140‑1 (MQ=255)

   cTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑T‑c‑c‑cc                                                                                                                                    <  1:3590170/140‑1 (MQ=255)

   cTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGAGCAC‑T‑c‑c‑cc                                                                                                                                    <  2:5096840/140‑1 (MQ=255)

    tGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑T‑c‑c‑ccc                                                                                                                                   <  2:4035/140‑2 (MQ=255)

      aCCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑T‑c‑c‑ccctg                                                                                                                                 >  1:1654043/1‑137 (MQ=255)

       ccTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑T‑c‑c‑ccctgc                                                                                                                                <  2:3725190/140‑5 (MQ=255)

       ccTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑T‑c‑c‑ccctgc                                                                                                                                <  1:3423605/140‑5 (MQ=255)

         tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCt                                                                                                                               <  1:1322240/140‑1 (MQ=255)

         tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCt                                                                                                                               <  1:655613/140‑1 (MQ=255)

         tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCt                                                                                                                               <  1:219546/140‑1 (MQ=255)

         tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCt                                                                                                                               <  2:1788060/140‑1 (MQ=255)

         tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCt                                                                                                                               <  2:877505/140‑1 (MQ=255)

         tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCt                                                                                                                               <  1:981632/140‑1 (MQ=255)

         tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCt                                                                                                                               <  2:78832/140‑1 (MQ=255)

         tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCt                                                                                                                               <  2:482341/140‑1 (MQ=255)

         tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCt                                                                                                                               <  2:3198132/140‑1 (MQ=255)

         tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCt                                                                                                                               <  1:4997036/140‑1 (MQ=255)

         tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTACCCGCATAGTATCAGGTGCGC‑T‑CAC‑CCTGCCt                                                                                                                               <  2:3069222/140‑1 (MQ=255)

          gcgcCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTc                                                                                                                              <  1:4666434/140‑1 (MQ=255)

           cgcCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCa                                                                                                                             <  1:476638/140‑1 (MQ=255)

           cgcCAACATGCGTTAAGTCTTGTACTGAGTGGCCAACCGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTATTTCTCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCa                                                                                                                             >  1:4564379/1‑140 (MQ=255)

            gcCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCAc                                                                                                                            <  1:1960330/140‑1 (MQ=255)

            gcCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCAc                                                                                                                            <  2:6700/140‑1 (MQ=255)

            gcCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCAc                                                                                                                            <  1:333359/140‑1 (MQ=255)

            gcCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCAc                                                                                                                            <  1:3786727/140‑1 (MQ=255)

               aaCATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGta                                                                                                                         <  2:4872979/140‑1 (MQ=255)

                     cGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACt                                                                                                                   >  2:4718277/1‑140 (MQ=255)

                     cGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACt                                                                                                                   >  1:1391111/1‑140 (MQ=255)

                           gTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGct                                                                                                             <  1:5099583/140‑1 (MQ=255)

                               tGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCttt                                                                                                         >  1:4111448/1‑140 (MQ=255)

                               tGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGGTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCttt                                                                                                         <  2:2767120/140‑1 (MQ=255)

                                gTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTc                                                                                                        >  1:1583572/1‑140 (MQ=255)

                                   cTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGat                                                                                                     <  2:4179581/140‑1 (MQ=255)

                                    tGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGata                                                                                                    <  1:1907876/140‑1 (MQ=255)

                                    tGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGata                                                                                                    <  1:2365382/140‑1 (MQ=255)

                                      aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                  >  1:3808725/1‑140 (MQ=255)

                                      aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                  >  2:2972078/1‑140 (MQ=255)

                                      aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                  >  2:1543824/1‑140 (MQ=255)

                                      aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                  >  2:4256622/1‑140 (MQ=255)

                                       ttGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATAtca                                                                                                 <  2:1055455/139‑1 (MQ=255)

                                         ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                               <  2:2157383/140‑1 (MQ=255)

                                         ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                               <  2:4349429/140‑1 (MQ=255)

                                         ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                               <  2:4642691/140‑1 (MQ=255)

                                         ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                               <  1:1672790/140‑1 (MQ=255)

                                         cgCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                               <  2:1871828/139‑1 (MQ=255)

                                           ccGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATGTTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcattca                                                                                             >  1:3143889/1‑140 (MQ=255)

                                           ccGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcattca                                                                                             >  2:2524118/1‑140 (MQ=255)

                                           ccGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcattca                                                                                             <  2:4776763/140‑1 (MQ=255)

                                           ccGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcattca                                                                                             >  1:1048607/1‑140 (MQ=255)

                                           ccGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcattca                                                                                             <  1:1858790/140‑1 (MQ=255)

                                           ccGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcattca                                                                                             >  1:1062049/1‑140 (MQ=255)

                                                aGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCCTTCatatca                                                                                        >  2:5009859/1‑140 (MQ=255)

                                                                tattTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGaa                                                                        <  2:2005662/140‑1 (MQ=255)

                                                                tattTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGaa                                                                        <  2:3808725/140‑1 (MQ=255)

                                                                 attGTTTTCGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATTCGCATAGTATCAGGTGCGC‑T‑C‑CTCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCGTATCAATATCATTCAAAAGaaa                                                                       <  2:1967414/140‑1 (MQ=255)

                                                                        aCGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAAttt                                                                >  1:407102/1‑140 (MQ=255)

                                                                          gTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTAc                                                              <  2:1614273/140‑1 (MQ=255)

                                                                           tCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCCTAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACg                                                             <  2:1504844/140‑1 (MQ=255)

                                                                           tCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACg                                                             <  2:4975119/140‑1 (MQ=255)

                                                                                    ccTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTaca                                                    <  2:102667/140‑1 (MQ=255)

                                                                                         tCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACAcgggc                                               <  2:2233924/140‑1 (MQ=255)

                                                                                             gCTGTTTCGCCCCTTATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGa                                           <  1:917874/140‑1 (MQ=255)

                                                                                             gCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGa                                           >  2:2118956/1‑140 (MQ=255)

                                                                                              cTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGAc                                          >  1:2798414/1‑140 (MQ=255)

                                                                                                  ttCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATTATTCATATCAATATCATGCAAAAGAAATCAATTTACTTTGCCTACACGGGCGGGACCAtt                                      >  1:2990585/1‑139 (MQ=255)

                                                                                                  ttCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATc                                      >  2:356409/1‑140 (MQ=255)

                                                                                                  ttCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATc                                      <  2:1662502/140‑1 (MQ=255)

                                                                                                      ccccATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGa                                  >  1:2049919/1‑140 (MQ=255)

                                                                                                         cATATTTCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATgt                               >  1:4502753/1‑139 (MQ=255)

                                                                                                               tCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTCTATACTTGTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGtt                         <  2:3473191/140‑1 (MQ=255)

                                                                                                               tCCTTATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGtt                         <  1:1475554/140‑1 (MQ=255)

                                                                                                                   tctacGCCTAGCATCAGGTGCGCGC‑C‑C‑CCTGCCTCACGTAGATACTTTTGCTCTTTCGCTATCATTCATATCAAGATCATGCAAAAGAAATCAAGGTACGTTGCCTACACGGGCGGGACTCTCGGGATGCTGCGTTcctg                     >  2:561608/5‑138 (MQ=255)

                                                                                                                   tATCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGa                     >  2:4448119/1‑140 (MQ=255)

                                                                                                                    gtCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTCTGATCTTTCGATATCATCCAAATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACTGGCGGGTCCATCGGGATGCAGCGTTCCGAg                    <  1:3058656/139‑1 (MQ=255)

                                                                                                                    aTCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg                    <  2:3117769/140‑1 (MQ=255)

                                                                                                                    aTCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg                    <  1:1433526/140‑1 (MQ=255)

                                                                                                                    aTCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg                    <  2:2366028/140‑1 (MQ=255)

                                                                                                                    aTCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg                    <  2:1313106/140‑1 (MQ=255)

                                                                                                                     tCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCACAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGc                   >  1:4254991/1‑140 (MQ=255)

                                                                                                                     tCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTTCCTACACGGGCGGGACCATCCGGATGCTTCGGTCCGAGc                   >  2:1228036/1‑140 (MQ=255)

                                                                                                                     tCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCa                              <  1:4155338/129‑1 (MQ=255)

                                                                                                                     tCCGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGc                   >  1:4618811/1‑140 (MQ=255)

                                                                                                                     tCCGAATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGc                   >  1:101117/1‑140 (MQ=255)

                                                                                                                      ccGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                  <  1:2459288/140‑1 (MQ=255)

                                                                                                                      ccGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                  <  1:136542/140‑1 (MQ=255)

                                                                                                                      ccGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                  <  1:1203593/140‑1 (MQ=255)

                                                                                                                      ccGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                  <  2:2049919/140‑1 (MQ=255)

                                                                                                                       cGCATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTGGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAg                 <  2:4100652/140‑1 (MQ=255)

                                                                                                                         cATAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAggg               <  1:4449438/140‑1 (MQ=255)

                                                                                                                          aTAGTATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAgggg              >  1:1834200/1‑139 (MQ=255)

                                                                                                                              tATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATCTCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTtata          >  1:4389170/1‑140 (MQ=255)

                                                                                                                              tATCAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTtata          >  2:2912051/1‑140 (MQ=255)

                                                                                                                                 cAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATAc       <  1:349587/140‑1 (MQ=255)

                                                                                                                                 cAGGTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATAc       <  2:2798414/140‑1 (MQ=255)

                                                                                                                                   ggTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCg     >  1:2594391/1‑140 (MQ=255)

                                                                                                                                   ggTGCGC‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCg     >  2:2650976/1‑140 (MQ=255)

                                                                                                                                      gcgc‑TCC‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGgtg  >  1:3277355/1‑140 (MQ=255)

TGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGC‑T‑C‑C‑CCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGGTG  >  NZ_CP025268/1855483‑1855755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`