BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,873,836	(T)_5→6	pseudogene (67/1283 nt)	yeaH →	YeaH/YhbH family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,873,831	1	.	T	93.9%	992.3 / 47.8	282	pseudogene (62/1283 nt)	yeaH	YeaH/YhbH family protein
Reads supporting (aligned to +/- strand): ref base . (10/7); new base T (159/104); total (171/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 330 total aligned reads displayed.

AACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑TT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATC  >  NZ_CP025268/1873693‑1873964

    aCAGCGACCCGTGGGCGTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGGCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑ct‑t‑t‑t                                                                                                                                  >  1:3081779/1‑140 (MQ=255)

    aCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACTGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑t‑t‑t                                                                                                                                  >  1:5044854/1‑140 (MQ=255)

    aCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑t‑t‑t                                                                                                                                  >  2:4681315/1‑140 (MQ=255)

     cAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑t‑t‑tt                                                                                                                                 >  1:2276365/1‑139 (MQ=255)

      aGCGAACCGTGGGCCTGAGAAGCGGCCACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑t‑t‑tta                                                                                                                                <  1:2626670/140‑3 (MQ=255)

      aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑t‑t‑tta                                                                                                                                <  1:3358650/140‑3 (MQ=255)

        cggaCCGTGGGCCTGAGAAGCGGAAACACAGGCGTAGCATACAGTTGGCAACTGTAGTACGGGGGGCATATGACCTGGTTTATTGCCCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑t‑t‑ttacg                                                                                                                              <  1:550306/137‑5 (MQ=255)

        cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACATTTCGCAAATGTAGGACGGGAGGCATATGACCTGGTTTATTGACCGGCCTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑t‑t‑ttacg                                                                                                                              <  2:1289791/140‑5 (MQ=255)

        cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑t‑t‑ttacg                                                                                                                              <  1:1272279/140‑5 (MQ=255)

        cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑t‑t‑ttacg                                                                                                                              <  1:5105911/140‑5 (MQ=255)

        cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑t‑t‑ttacg                                                                                                                              <  2:3896390/140‑5 (MQ=255)

        cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTCACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑tt‑t‑t‑ttacg                                                                                                                              <  2:1357601/140‑5 (MQ=255)

          aaCCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGATGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GATT‑T‑T‑TACAcc                                                                                                                             >  1:418822/1‑140 (MQ=255)

               tGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTtat                                                                                                                        >  2:3932592/1‑140 (MQ=255)

               tGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTtat                                                                                                                        >  2:4561411/1‑140 (MQ=255)

                    cTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAgc                                                                                                                   <  1:1437547/140‑1 (MQ=255)

                    cTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAgc                                                                                                                   <  1:4911167/140‑1 (MQ=255)

                    cTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAgc                                                                                                                   <  1:4120929/140‑1 (MQ=255)

                       agaAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGG‑TT‑T‑T‑TACGCCGTTATAAAGCTCa                                                                                                                >  2:2130997/1‑140 (MQ=255)

                       agaAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCa                                                                                                                >  2:2088229/1‑140 (MQ=255)

                            cGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTa                                                                                                           >  2:3529482/1‑140 (MQ=255)

                            cGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTa                                                                                                           >  2:737372/1‑140 (MQ=255)

                            cGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTa                                                                                                           >  1:3584929/1‑140 (MQ=255)

                            cGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑TT‑T‑TGTACGCCGTTATAAAGCGCAAATTa                                                                                                           >  1:393229/1‑140 (MQ=255)

                              gCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTaaa                                                                                                         <  2:1205347/140‑1 (MQ=255)

                              gCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTaaa                                                                                                         <  1:1037301/140‑1 (MQ=255)

                              gCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTaaa                                                                                                         <  2:4456737/140‑1 (MQ=255)

                                 acacAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGCATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCACATTAAACAg                                                                                                      >  1:4323294/1‑140 (MQ=255)

                                  cacaGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGt                                                                                                     <  2:875286/140‑1 (MQ=255)

                                    caGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCg                                                                                                   <  1:3379556/140‑1 (MQ=255)

                                     aGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTAGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGa                                                                                                  <  2:4191586/140‑1 (MQ=255)

                                          tAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGCACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTcc                                                                                             <  2:4350055/140‑1 (MQ=255)

                                          tAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTcc                                                                                             >  2:158385/1‑140 (MQ=255)

                                           aGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCg                                                                                            <  1:2498176/140‑1 (MQ=255)

                                            gCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGa                                                                                           <  2:372537/140‑1 (MQ=255)

                                             cATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAg                                                                                          >  1:4714094/1‑140 (MQ=255)

                                              aTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                         <  1:1492633/140‑1 (MQ=255)

                                                 cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                                      >  1:2185085/1‑140 (MQ=255)

                                                 cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                                      >  2:1197416/1‑140 (MQ=255)

                                                      ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAACAACAAAAGCATGGTGAAGCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                                 <  1:691617/140‑1 (MQ=255)

                                                      ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                                 >  2:133933/1‑140 (MQ=255)

                                                      ggCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACCGCAAAAACAAAAGCATGGTGAATCGCCAGA‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaat                                                                                 <  2:1141496/140‑1 (MQ=255)

                                                       gCAAATGTAGTTCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCGAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaata                                                                                >  1:4236107/1‑140 (MQ=255)

                                                            tGTAGTACGGGGGGCATATGACCTGGTTTATTGACTGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGt                                                                           >  2:4642324/1‑140 (MQ=255)

                                                            tGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGt                                                                           >  2:1618772/1‑140 (MQ=255)

                                                            tGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGt                                                                           >  2:1838813/1‑140 (MQ=255)

                                                            tGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGt                                                                           >  2:2751795/1‑140 (MQ=255)

                                                              tagtaCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTc                                                                         >  2:3421996/1‑140 (MQ=255)

                                                                   cGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtga                                                                    <  2:2276365/140‑1 (MQ=255)

                                                                   cGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtga                                                                    <  2:1414202/140‑1 (MQ=255)

                                                                   cGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtga                                                                    <  2:3568301/140‑1 (MQ=255)

                                                                     gggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgact                                                                  >  1:3703601/1‑140 (MQ=255)

                                                                      ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                 >  2:3481390/1‑140 (MQ=255)

                                                                      ggggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactg                                                                 >  2:655269/1‑140 (MQ=255)

                                                                        ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactgac                                                               >  1:209700/1‑140 (MQ=255)

                                                                        ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactgac                                                               >  1:1935193/1‑140 (MQ=255)

                                                                        ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactgaa                                                               >  2:1489456/1‑139 (MQ=255)

                                                                        ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGCCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactgac                                                               >  2:4982215/1‑140 (MQ=255)

                                                                           atatGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTc                                                            >  1:1598624/1‑140 (MQ=255)

                                                                           atatGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTc                                                            >  1:4567383/1‑140 (MQ=255)

                                                                           atatGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTc                                                            >  1:4849166/1‑140 (MQ=255)

                                                                                aCCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAg                                                       <  2:4075464/140‑1 (MQ=255)

                                                                                aCCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAg                                                       >  1:3905716/1‑140 (MQ=255)

                                                                                aCCTGGTTTATTGACCGGCGTCTGAACGACAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAg                                                       >  2:3340332/1‑140 (MQ=255)

                                                                                  cTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcg                                                     >  1:3756803/1‑140 (MQ=255)

                                                                                    ggTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggc                                                   >  2:1434534/1‑140 (MQ=255)

                                                                                    ggTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑TT‑T‑TCTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCCCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggc                                                   >  2:3060644/1‑140 (MQ=255)

                                                                                     gTTTATTGGCCGGCGTCTGAACGGCAAAAACAAACGCATGGTGAATCGCCAGG‑G‑TTGT‑T‑TACGCCGTTATAAAGCGCAAAGTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  <  2:1962524/140‑1 (MQ=255)

                                                                                     gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  >  2:4157199/1‑140 (MQ=255)

                                                                                     gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  <  2:3788221/140‑1 (MQ=255)

                                                                                     gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  <  1:4116289/140‑1 (MQ=255)

                                                                                     gTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                  >  2:4847526/1‑140 (MQ=255)

                                                                                        tATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TATGCCGGTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAAt                                               >  1:2756174/1‑140 (MQ=255)

                                                                                        tATTGACCGGCGTCTGAACGACAAAAACAAAACCATGGTGAATCTCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGCGGCCATTAATACGCGTTCGGTGACTGACGTCGACAGCGGCGAAt                                               <  2:244314/140‑1 (MQ=255)

                                                                                          ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc                                             >  1:2323663/1‑140 (MQ=255)

                                                                                          ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc                                             >  1:3157821/1‑140 (MQ=255)

                                                                                          ttGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATcc                                             >  2:4946569/1‑140 (MQ=255)

                                                                                           tGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCg                                            >  2:4381638/1‑140 (MQ=255)

                                                                                               cggcggCTGAACGGCAAAAGCAAAAGCATGGGGACTCGCCCGCGG‑TT‑G‑T‑TACGCCGTTATAAAGCGCAAGTTATACTGTCGATTCCCGAGGCCAGTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                        <  2:2657281/140‑1 (MQ=255)

                                                                                               cGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑TT‑TGT‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACATCGACAGCGGCGAATCCGTATc                                        >  2:2831527/1‑140 (MQ=255)

                                                                                                    tCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTc                                   >  1:1871928/1‑140 (MQ=255)

                                                                                                    ccTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGGCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCAtcc                                   >  2:106744/2‑138 (MQ=255)

                                                                                                     cTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTcc                                  <  1:3968918/140‑1 (MQ=255)

                                                                                                       gAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCa                                <  1:133933/140‑1 (MQ=255)

                                                                                                           ggCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGa                            <  1:3256132/140‑1 (MQ=255)

                                                                                                            gCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGaa                           >  1:886944/1‑140 (MQ=255)

                                                                                                            gCAAAAACAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTGATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGaa                           >  1:4947809/1‑140 (MQ=255)

                                                                                                              aaaaaCAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGa                         <  1:3821268/140‑1 (MQ=255)

                                                                                                                aaaCAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGata                       <  1:4838022/140‑1 (MQ=255)

                                                                                                                 aaCAAAAGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGatat                      <  2:2268404/140‑1 (MQ=255)

                                                                                                                     aaaGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGc                  >  2:2043186/1‑140 (MQ=255)

                                                                                                                      aaGCATGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCg                 >  2:2551648/1‑140 (MQ=255)

                                                                                                                          aTGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAAcc             <  1:2786239/140‑1 (MQ=255)

                                                                                                                          aTGGTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAAcc             <  2:4759456/140‑1 (MQ=255)

                                                                                                                          aTGGTGAATCGCCAGA‑GTTT‑T‑T‑TACGCCGTTATAAAGCGGAAATTAAACAGTCGATCTGCGAGGCCAGTAATAAGCGTCCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAAcc             <  2:1703270/140‑1 (MQ=255)

                                                                                                                             gTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGAt          <  1:1641228/140‑1 (MQ=255)

                                                                                                                             gTGAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCGCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATGAGTAACCATTCCCACGGAAGAGATTAGCGAACCGAt          >  2:3153212/1‑140 (MQ=255)

                                                                                                                               gAATCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGt        <  2:2963749/140‑1 (MQ=255)

                                                                                                                                  tCGCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTtc     >  1:3505691/1‑140 (MQ=255)

                                                                                                                                    gCCAGC‑GTTT‑T‑T‑TACGCCGTTATAAAGCGCAAATGAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTtcat   <  1:2963637/140‑1 (MQ=255)

AACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGC‑G‑TT‑T‑T‑TACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATC  >  NZ_CP025268/1873693‑1873964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`