BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,913,492	+T	coding (88/144 nt)	mgrB ←	PhoP/PhoQ regulator MgrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,913,492	1	.	T	98.8%	909.8 / 1.9	253	coding (88/144 nt)	mgrB	PhoP/PhoQ regulator MgrB
Reads supporting (aligned to +/- strand): ref base . (3/0); new base T (148/99); total (152/101)
Fisher's exact test for biased strand distribution p-value = 2.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 262 total aligned reads displayed.

TCGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTG‑ATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCGT  >  NZ_CP025268/1913354‑1913625

 cGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAGTGGCACAAATTCCGCTGAAAAATTGTACATCCTgta                                                                                                                                      >  2:2763039/1‑138 (MQ=255)

 cGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTgta                                                                                                                                      >  1:2588185/1‑138 (MQ=255)

   ttCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTgtatc                                                                                                                                    <  1:4442852/140‑5 (MQ=255)

        ttCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGcaca                                                                                                                               <  2:5008814/140‑1 (MQ=255)

        ttCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGcaca                                                                                                                               <  1:3302958/140‑1 (MQ=255)

        ttCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGcaca                                                                                                                               <  1:2080175/140‑1 (MQ=255)

          caccccCTCTGAGTAGCGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCAcctc                                                                                                                             <  2:3129298/140‑1 (MQ=255)

          caccacCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCAcatc                                                                                                                             <  1:1092180/140‑1 (MQ=255)

          caccacCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCAcatc                                                                                                                             <  2:4516231/140‑1 (MQ=255)

          caccacCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCAcatc                                                                                                                             <  1:4803897/140‑1 (MQ=255)

          caccacCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCAcatc                                                                                                                             <  2:444940/140‑1 (MQ=255)

                ctctGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTg                                                                                                                       >  2:354494/1‑140 (MQ=255)

                ctctGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTg                                                                                                                       >  2:1652060/1‑140 (MQ=255)

                  ctGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGaa                                                                                                                     >  2:4619216/1‑140 (MQ=255)

                  ctGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAAGCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTGCATCCTGTATCGCACATCATGTTGaa                                                                                                                     >  2:4398148/1‑140 (MQ=255)

                     aGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATAc                                                                                                                  >  2:3718993/1‑140 (MQ=255)

                     aGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATAc                                                                                                                  >  1:1756435/1‑140 (MQ=255)

                       tAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCt                                                                                                                <  2:4472016/140‑1 (MQ=255)

                       tAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCt                                                                                                                <  2:189583/140‑1 (MQ=255)

                       tAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCt                                                                                                                >  1:1695085/1‑140 (MQ=255)

                       tAGAGGCGCTAGTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCt                                                                                                                <  1:3031641/140‑1 (MQ=255)

                          agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  1:1903263/1‑140 (MQ=255)

                          agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  1:99275/1‑140 (MQ=255)

                          agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  2:2265170/1‑140 (MQ=255)

                          agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  2:2850101/1‑140 (MQ=255)

                          agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  1:2222054/1‑140 (MQ=255)

                          agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  2:4936078/1‑140 (MQ=255)

                          agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGGTGAATACCTgcg                                                                                                             >  2:188767/1‑140 (MQ=255)

                          agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTAGCGCACATCATGTTGAATACCTgcc                                                                                                             >  2:4877822/1‑139 (MQ=255)

                            gcgcTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGcc                                                                                                           >  2:4310358/1‑140 (MQ=255)

                            gcgcTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGcc                                                                                                           >  2:1143788/1‑140 (MQ=255)

                            gcgcTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGcc                                                                                                           >  2:599313/1‑140 (MQ=255)

                               cTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAGATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCaa                                                                                                        >  1:4261210/1‑140 (MQ=255)

                               cTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCaa                                                                                                        >  2:5099972/1‑140 (MQ=255)

                               cTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCaa                                                                                                        >  2:710115/1‑140 (MQ=255)

                               cTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCaa                                                                                                        >  1:2478909/1‑140 (MQ=255)

                               cTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCaa                                                                                                        >  1:1515322/1‑140 (MQ=255)

                               cTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCaa                                                                                                        >  1:4299262/1‑140 (MQ=255)

                               cTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAACGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCaa                                                                                                        >  2:161217/1‑140 (MQ=255)

                                 aTTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAag                                                                                                      >  2:3966328/1‑140 (MQ=255)

                                 aTTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAag                                                                                                      >  2:1829526/1‑140 (MQ=255)

                                   tCTACCACTGCTTGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATCTCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACACCATGTTGAATACCTGCGCCCAAagca                                                                                                    <  2:772343/140‑1 (MQ=255)

                                      aCCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAgcagca                                                                                                 >  1:1186653/1‑140 (MQ=255)

                                       ccACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaa                                                                                                >  2:199556/1‑140 (MQ=255)

                                          ctgctgGAGAGTAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaagca                                                                                             >  1:1611386/1‑140 (MQ=255)

                                          ctgctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTTCGCCCAAAGCagcaagca                                                                                             >  2:4154709/1‑140 (MQ=255)

                                          ctgctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaagca                                                                                             >  1:3089500/1‑140 (MQ=255)

                                            gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACGTGCGCCCAAAGCAGcaagcaag                                                                                           >  2:948838/1‑140 (MQ=255)

                                            gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  1:397236/1‑140 (MQ=255)

                                            gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  1:789635/1‑140 (MQ=255)

                                            gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  1:4992379/1‑140 (MQ=255)

                                            gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  1:5107436/1‑140 (MQ=255)

                                            gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  1:4422528/1‑140 (MQ=255)

                                            gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTG‑ATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCaagcaagc                                                                                          >  1:1003984/1‑140 (MQ=255)

                                            gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTG‑ATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCaagcaagc                                                                                          >  1:791391/1‑140 (MQ=255)

                                             ctgGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCaagcaagc                                                                                          >  2:3253008/1‑140 (MQ=255)

                                                     gaagaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAAcacca                                                                                  >  2:778010/1‑140 (MQ=255)

                                                     gaagaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAAcacca                                                                                  >  1:1987350/1‑140 (MQ=255)

                                                     gaagaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAAcacca                                                                                  >  1:2542166/1‑140 (MQ=255)

                                                     gaagaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAAcacca                                                                                  >  1:2663887/1‑140 (MQ=255)

                                                       aggaaaTCTAGGGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATGGTACATCCTGTATCGAACATCATGTTGAATAACTGAGCCAAAAGAAGCAAGAAATCCAACGCCACg                                                                                >  2:530242/4‑140 (MQ=255)

                                                       agacAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACg                                                                                <  1:3629025/140‑1 (MQ=255)

                                                       agaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAAcaccaaa                                                                                >  2:3890814/1‑138 (MQ=255)

                                                       agaaAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACg                                                                                <  2:1947044/140‑1 (MQ=255)

                                                            aTCTAGTGCTGAAAAAACGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAACACCTGCGCCCCAAGCAGCAAGCAATCCAACACCACGACAAc                                                                           <  2:3077504/140‑1 (MQ=255)

                                                                  tGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATGCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCGGAGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGaag                                                                     >  2:2208642/1‑139 (MQ=255)

                                                                                 atCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACtt                                                      >  1:2952078/1‑140 (MQ=255)

                                                                                      cacGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAATATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCAACCGAAACTTTTTcc                                                 >  2:4596112/1‑139 (MQ=255)

                                                                                           gATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTGTTCACTCCa                                            <  2:479797/140‑1 (MQ=255)

                                                                                            aTAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCaa                                           >  2:2712715/1‑140 (MQ=255)

                                                                                              aaaCTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAAtc                                         <  2:2545764/140‑1 (MQ=255)

                                                                                                   ggTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGt                                    <  1:3691968/140‑1 (MQ=255)

                                                                                                    gTTAATGGCACATATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGtt                                   >  2:5035850/1‑140 (MQ=255)

                                                                                                    gTTAATGGCACAAATTCCGCTGAGAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTACGtt                                   >  1:2989192/1‑140 (MQ=255)

                                                                                                    gTTAATGGCACAAATTCCGCTGAAAAATTGTAGATCCTGTATGGCGCATCATGTTGAATACCTGCGCGCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCTAAACTTTTTCACTCCAATCTCCGtt                                   >  2:3035289/1‑140 (MQ=255)

                                                                                                     ttAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGttt                                  >  1:4044380/1‑140 (MQ=255)

                                                                                                     ttAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGttt                                  >  2:1097151/1‑140 (MQ=255)

                                                                                                     ttAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCCCCGttt                                  >  1:2878936/1‑140 (MQ=255)

                                                                                                       aaTGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCa                                >  1:1365720/1‑140 (MQ=255)

                                                                                                       aaTGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCa                                <  1:3756217/140‑1 (MQ=255)

                                                                                                        attatccaAATTCCGCTGAAAAATTGTACATCCAGTATAGCACACCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAGCACCACGACTACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac                               <  2:1502289/134‑1 (MQ=255)

                                                                                                        aTGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac                               <  2:3596701/140‑1 (MQ=255)

                                                                                                        aTGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac                               <  1:4535004/140‑1 (MQ=255)

                                                                                                        aTGGCACAAATACCGCTGAACAATTGTACATCCTGTATCGCACATCATGTTGAAAACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac                               <  2:1308580/140‑1 (MQ=255)

                                                                                                         tGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacc                              >  1:3930356/1‑140 (MQ=255)

                                                                                                         tGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacc                              >  1:1273632/1‑140 (MQ=255)

                                                                                                         tGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacc                              <  1:4921815/140‑1 (MQ=255)

                                                                                                         tGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacc                              >  1:3110415/1‑140 (MQ=255)

                                                                                                             acaAAGTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacctacc                          <  2:2042520/140‑1 (MQ=255)

                                                                                                              caAATTCCGCTGAAAAATTTTACATCCTGTATCGCACATCTTGTTGAGTACCGGCGTCCATAGCATCAATCAAGCCAAGAGTTCGACAACCAGAACGACCCATCTAAACTTTTTCACGCGAATCTCCGTTTCacctacat                         >  2:2830231/1‑138 (MQ=255)

                                                                                                              caAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacctacct                         >  2:3303231/1‑140 (MQ=255)

                                                                                                              caAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacctacct                         >  1:3830692/1‑140 (MQ=255)

                                                                                                                 aTTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTAt                      <  1:724436/140‑1 (MQ=255)

                                                                                                                     cGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCa                  <  1:599313/140‑1 (MQ=255)

                                                                                                                     cGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCa                  <  2:1407821/140‑1 (MQ=255)

                                                                                                                                   aCATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCACGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGc    <  2:1713116/140‑1 (MQ=255)

                                                                                                                                   aCATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGc    <  2:402945/140‑1 (MQ=255)

                                                                                                                                   aCATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGc    <  2:1276686/140‑1 (MQ=255)

                                                                                                                                     aTCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACATTATGTCATTAAACTAGCATGCgt  <  2:300991/140‑1 (MQ=255)

                                                                                                                                     aTCCTGTATCGCAAATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCgt  <  2:3823928/140‑1 (MQ=255)

TCGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTGAAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTG‑ATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCGT  >  NZ_CP025268/1913354‑1913625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`