BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,218,374	(C)_5→6	pseudogene (306/1685 nt)	btsS ←	two‑component regulatory system sensor histidine kinase BtsS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,218,369	1	.	C	97.3%	728.0 / 11.2	223	pseudogene (311/1685 nt)	btsS	two‑component regulatory system sensor histidine kinase BtsS
Reads supporting (aligned to +/- strand): ref base . (4/2); new base C (131/85); total (136/87)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 243 total aligned reads displayed.

CAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑G‑C‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAAC  >  NZ_CP025268/2218230‑2218503

   cgacgaACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑G‑c‑cc                                                                                                                                     >  2:2280635/1‑140 (MQ=255)

    gacgaACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑G‑c‑ccc                                                                                                                                    <  1:1520395/140‑1 (MQ=255)

    gacgaACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑G‑c‑ccc                                                                                                                                    <  2:4893384/140‑1 (MQ=255)

    gacgaACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑G‑c‑ccc                                                                                                                                    <  2:3926921/140‑1 (MQ=255)

    gacgaACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑G‑c‑ccc                                                                                                                                    <  2:5096059/140‑1 (MQ=255)

      cgaACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑G‑c‑ccccc                                                                                                                                  <  1:2367270/140‑2 (MQ=255)

       gaACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑G‑c‑ccccca                                                                                                                                 <  2:4943109/140‑3 (MQ=255)

        aaCGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑G‑c‑cccccat                                                                                                                                >  1:3214906/1‑137 (MQ=255)

        aaCGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑G‑c‑cccccat                                                                                                                                >  2:1860971/1‑137 (MQ=255)

           gTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGa                                                                                                                              <  1:3397466/140‑1 (MQ=255)

               cGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAatat                                                                                                                          <  1:4405646/140‑1 (MQ=255)

               cGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAatat                                                                                                                          <  2:1140835/140‑1 (MQ=255)

                  cACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGa                                                                                                                       >  1:2989811/1‑140 (MQ=255)

                          gTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAgcc                                                                                                               <  1:985203/140‑1 (MQ=255)

                            aaTGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAgccgc                                                                                                             >  1:2026367/1‑140 (MQ=255)

                             aTGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAgccgcc                                                                                                            >  2:3813668/1‑140 (MQ=255)

                               ggggTTAACGACTTTATCAGTGCACCCGCGTCGGATCAGGATGATGCGTACCATGCCACCGCCTAATCCTACAGCGATGGTCGAGATCAAGCCACTTAACGCGGTCATAC‑C‑CCCCATCGAATATCGATGTAAGCCGCCgg                                                                                                          <  2:3898043/140‑1 (MQ=255)

                               ggggTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCgg                                                                                                          <  2:1510212/140‑1 (MQ=255)

                               ggggTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCgg                                                                                                          <  1:1722309/140‑1 (MQ=255)

                               ggggTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCgg                                                                                                          <  2:4292943/140‑1 (MQ=255)

                                  gTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCa                                                                                                       >  1:2439612/1‑140 (MQ=255)

                                  gTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGGCGAGGTCATGCAACTTAACGCGGTCAT‑GCC‑CCCCAGCGAATATCGATGTAAGCCGCCGGGCa                                                                                                       >  2:2423578/1‑140 (MQ=255)

                                     aaaaACTTTATCAGTGCGCCCGCGGCGAATCAGGAAGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGCTGGTCTAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATCTCTACGTAAGCCGCCGGTCAGcc                                                                                                    >  2:830050/1‑140 (MQ=255)

                                     aaaGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATTGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGcc                                                                                                    >  1:902376/1‑140 (MQ=255)

                                     aaaGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGcc                                                                                                    >  1:2542017/1‑140 (MQ=255)

                                     aaaGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGcc                                                                                                    >  2:4180894/1‑140 (MQ=255)

                                           tttATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCTTTCAACGATGGTCGAGATCATGCAACATAACGCGGTCAT‑GTC‑CCCCATCGGATTTCGATGTAAGCCGCCTGTCAGCCCTAcct                                                                                              >  2:656401/1‑139 (MQ=255)

                                           tttATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCa                                                                                              <  1:3614429/140‑1 (MQ=255)

                                            ttATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAg                                                                                             >  2:3312716/1‑140 (MQ=255)

                                            ttATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑G‑CACCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAg                                                                                             >  1:4861899/1‑140 (MQ=255)

                                            ttATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGGCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAg                                                                                             >  1:1685080/1‑140 (MQ=255)

                                             tATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGGCCAACCAGc                                                                                            >  1:2122329/1‑140 (MQ=255)

                                             tATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGc                                                                                            >  1:1263872/1‑140 (MQ=255)

                                                  gTGCGCCCGCGGCGGATCAGGATGCTGTGTACCACGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCAcc                                                                                       <  2:4548927/140‑1 (MQ=255)

                                                   tGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACcg                                                                                      >  1:485641/1‑140 (MQ=255)

                                                   tGCGCCCGCGGCGGATCACGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGAGGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCTATGTAAGCCGCCGGTCAGCCCAACCAGCCCACcg                                                                                      >  2:1410528/1‑140 (MQ=255)

                                                     cgcCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACcgac                                                                                    >  1:3480786/1‑140 (MQ=255)

                                                       cccGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACcgacga                                                                                  >  2:233431/1‑140 (MQ=255)

                                                              cggATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACgaccggacc                                                                           <  1:2580924/140‑1 (MQ=255)

                                                                        tGCTGTGTACCAGGCCACCTAGTAAGCCTTCAACCATGCTCGAGATCATGCAACTTAACGCGCTCAT‑GCC‑CCCCCTCGAATTCCGCTGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                                 <  2:2125883/140‑1 (MQ=255)

                                                                        tGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATGTCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                                 <  1:271907/140‑1 (MQ=255)

                                                                        tGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                                 >  1:4114435/1‑140 (MQ=255)

                                                                        tGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGGCAGCCCAACCAGCACACCGACGACCGGACCGCCGAGTAAg                                                                 <  1:1786439/140‑1 (MQ=255)

                                                                          cTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAgcc                                                               <  1:2814818/140‑1 (MQ=255)

                                                                          cTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAgcc                                                               <  1:1446328/140‑1 (MQ=255)

                                                                          cTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAgcc                                                               >  2:2513671/1‑140 (MQ=255)

                                                                           tgtgTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAgccg                                                              >  2:4337848/1‑140 (MQ=255)

                                                                           tgtgTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAgccg                                                              >  1:1799896/1‑140 (MQ=255)

                                                                              gtACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGATTATCGATGTAAGCCGCCGGTCAGCCCAACCGGCCCACCGACGACCGGACAGCCGAGTAAgccgctc                                                           >  2:1969697/1‑138 (MQ=255)

                                                                                aCCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGc                                                                                            >  2:2747643/1‑105 (MQ=255)

                                                                                aCCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCAt                                                         >  1:3966433/1‑140 (MQ=255)

                                                                                 ccAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCAtt                                                        >  1:3443960/1‑140 (MQ=255)

                                                                                    ggCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTAcc                                                     >  1:3315234/1‑140 (MQ=255)

                                                                                    ggCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTAcc                                                     >  1:2071385/1‑140 (MQ=255)

                                                                                    ggCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTAcc                                                     >  2:1230604/1‑140 (MQ=255)

                                                                                     gCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcg                                                    >  1:2271035/1‑140 (MQ=255)

                                                                                     gCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcg                                                    >  1:4041314/1‑140 (MQ=255)

                                                                                     gCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGGCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTAcca                                                    >  2:3929744/1‑139 (MQ=255)

                                                                                       cACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCGGGTCAGCCCAACCAGCCCACCGACGACCGGACGGCCGAGTAAgcc                                                               >  2:4619969/1‑127 (MQ=255)

                                                                                       cACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcgcg                                                  >  1:3889426/1‑140 (MQ=255)

                                                                                       cACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTAAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcgcg                                                  >  1:1186621/1‑140 (MQ=255)

                                                                                       cACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAAGATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGGAACCCGCCCATTACcgcg                                                  >  1:3909932/1‑140 (MQ=255)

                                                                                         ccGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGcc                                                >  2:2624153/1‑140 (MQ=255)

                                                                                         ccGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGcc                                                >  1:4756100/1‑140 (MQ=255)

                                                                                          cGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGGCAGCCGAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCAATTAAAGCGcca                                               >  2:4459829/1‑139 (MQ=255)

                                                                                            aGTAATCCTTCAACGATGGTCGAGATCATGCAACTTATCGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTAt                                             <  2:4021680/140‑1 (MQ=255)

                                                                                                aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  2:3443960/140‑1 (MQ=255)

                                                                                                aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  1:712673/140‑1 (MQ=255)

                                                                                                aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  2:2207619/140‑1 (MQ=255)

                                                                                                aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  2:184664/140‑1 (MQ=255)

                                                                                                aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  1:2172823/140‑1 (MQ=255)

                                                                                                aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  1:766757/140‑1 (MQ=255)

                                                                                                aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  1:4640282/140‑1 (MQ=255)

                                                                                                aTCCTAAAACGACGGTCGAAATAATGCAACCTAACGCGGTCCT‑GCC‑CCCCACCCAATATCGATGTACCCCGCCGGTCAGCCCATCCAGCCCACCGACGCCCGAACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  2:4201694/140‑1 (MQ=255)

                                                                                                aTCCCTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCCGCCCACCGACGTCCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  1:494503/140‑1 (MQ=255)

                                                                                                      cAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTa                                   >  1:1114639/1‑140 (MQ=255)

                                                                                                      cAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTa                                   >  1:2966266/1‑140 (MQ=255)

                                                                                                      cAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTa                                   <  1:4937379/140‑1 (MQ=255)

                                                                                                      cAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTa                                   >  2:4045532/1‑140 (MQ=255)

                                                                                                      cAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCAATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACAGCCGAGTAAGCCGCCCATCACCGCGCATATCGCACGGGTa                                   >  2:4817547/1‑140 (MQ=255)

                                                                                                        aCGATGGTCGAGATCATGCATCTTAACGCGGTCATGG‑C‑CCCCATCGATTATCGATGTATGCCGCAGGTCAGCCCAACCAGCGCACCGACGACCGGATCGCCGAGTAAGCCGCCCATTACCGCTCTTATCGCATGGGTAtt                                 <  2:4680691/140‑1 (MQ=255)

                                                                                                           aTGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGc                              >  2:1686983/1‑140 (MQ=255)

                                                                                                           aTGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGGAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGc                              >  2:3039139/1‑140 (MQ=255)

                                                                                                            tGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGACAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCa                             >  1:3729154/1‑140 (MQ=255)

                                                                                                             ggTCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCaa                            >  2:44867/1‑140 (MQ=255)

                                                                                                               tCGAGATCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATa                          >  1:3090971/1‑140 (MQ=255)

                                                                                                                  agaTCATGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGaa                       <  1:4157641/140‑1 (MQ=255)

                                                                                                                       aTGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTc                  <  1:1719000/140‑1 (MQ=255)

                                                                                                                        tGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCa                 >  1:453972/1‑140 (MQ=255)

                                                                                                                        tGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCa                 >  2:2234614/1‑140 (MQ=255)

                                                                                                                        tGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCa                 <  1:2280908/140‑1 (MQ=255)

                                                                                                                        tGCAACTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCa                 >  1:1868516/1‑140 (MQ=255)

                                                                                                                             cTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAGTCGTCAAtgtg            <  2:3504080/140‑1 (MQ=255)

                                                                                                                             cTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAAtgtg            <  1:5041247/140‑1 (MQ=255)

                                                                                                                             cTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAAtgtg            <  2:3674297/140‑1 (MQ=255)

                                                                                                                             cTTAACGCGGTCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAAtgtg            <  1:2233434/140‑1 (MQ=255)

                                                                                                                                       tCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAAc  >  1:4454715/1‑140 (MQ=255)

                                                                                                                                       tCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAAc  >  1:3275048/1‑140 (MQ=255)

                                                                                                                                       tCAT‑GCC‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAAc  >  1:1010468/1‑140 (MQ=255)

                                                                                                                                       tCAT‑G‑CGCCCCATCGAATCTCGATCTAAGCCACAGGTCACCCCACCCACCCCCCCGACCGCCTGACCGCCGGGTAACCCCCCCATAACCGCGCCTATCGCACGGGTATTGGCAATAGAATCATCAATGTGCAACCCAAAc  >  2:3009972/1‑140 (MQ=255)

CAGCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑G‑C‑CCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAAC  >  NZ_CP025268/2218230‑2218503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`