BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,292,596	(C)_6→7	pseudogene (361/2531 nt)	yejO ←	autotransporter YejO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,292,590	1	.	C	96.2%	686.2 / 17.5	211	pseudogene (367/2531 nt)	yejO	autotransporter YejO
Reads supporting (aligned to +/- strand): ref base . (3/5); new base C (83/119); total (86/125)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 223 total aligned reads displayed.

ACCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GG‑C‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCG  >  NZ_CP025268/2292451‑2292723

aCCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑gg                                                                                                                                          <  2:35679/140‑1 (MQ=255)

aCCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑gg                                                                                                                                          <  1:2783345/140‑1 (MQ=255)

aCCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTCTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑gg                                                                                                                                          <  1:4657014/140‑1 (MQ=255)

      aCGGTTTGTTCCGAATACCTCCATGGCCCGGGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTATTCCTCCCTTTTCGATAGCGACAAAGAC‑GG‑c‑cccc‑c                                                                                                                                 >  1:2339041/1‑140 (MQ=255)

          tttGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GG‑c‑cccc‑ccatc                                                                                                                             >  2:4409226/1‑136 (MQ=255)

                ccGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTc                                                                                                                        <  2:3575779/140‑1 (MQ=255)

                  gAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCaa                                                                                                                      <  1:2755434/140‑1 (MQ=255)

                   aaTACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAAc                                                                                                                     >  2:4084711/1‑140 (MQ=255)

                   aaTACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCAACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAAc                                                                                                                     >  2:221788/1‑140 (MQ=255)

                   aaTACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATACAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAAc                                                                                                                     >  1:2268789/1‑140 (MQ=255)

                        cTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTa                                                                                                                >  2:2592040/1‑140 (MQ=255)

                             tGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCACCGATTAAgcgc                                                                                                           >  2:2192956/1‑140 (MQ=255)

                             tGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAgcgg                                                                                                           >  2:1642088/1‑139 (MQ=255)

                             tGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAgcgc                                                                                                           >  1:3456075/1‑140 (MQ=255)

                             tGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAgcgc                                                                                                           >  1:3373887/1‑140 (MQ=255)

                               gCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGcgccc                                                                                                         <  2:2044267/140‑1 (MQ=255)

                               gCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGcgccc                                                                                                         <  2:1897082/140‑1 (MQ=255)

                               gCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGcgccc                                                                                                         <  2:4595609/140‑1 (MQ=255)

                               gCCCGCGTGCTTGTTTTAATAGCACCGCCTGCTTTCTGATCGACCGCAAATGCAAATCCCCCCTCTTTATCCTCCAGTAGTCCTCCCTTTTCGATAGCGACACAGAC‑GGCC‑CCCC‑CATCTTTTTCAACTATTAAGcgccc                                                                                                         <  2:2992958/140‑1 (MQ=255)

                                 ccGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGcgcccgc                                                                                                       >  1:2817862/1‑140 (MQ=255)

                                  cgcgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGcgcccgcc                                                                                                      >  1:4761023/1‑140 (MQ=255)

                                   gcgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGCCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCt                                                                                                     >  2:2927170/1‑140 (MQ=255)

                                   gcgTGGTTGTTTGAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCt                                                                                                     >  2:5058106/1‑140 (MQ=255)

                                    cgTTGTTGGTTTAATAGCACTGCCTGCTTTCTGATCTACCGCAAATGCAAATCCGCCCCCTTTAACCTCCAGAAGTCCGCCCTTTTCGAGAGCGACAAAGACGGG‑C‑CCCC‑CCCCTTTTTCAACGATTAAGcgcccgccct                                                                                                    >  2:2266774/1‑138 (MQ=255)

                                    cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCtt                                                                                                    >  2:1463821/1‑140 (MQ=255)

                                    cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCtt                                                                                                    >  1:97439/1‑140 (MQ=255)

                                    cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCtt                                                                                                    >  2:1175183/1‑140 (MQ=255)

                                     gTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCttt                                                                                                   <  1:746271/140‑1 (MQ=255)

                                            ttttAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTg                                                                                            <  1:220233/140‑1 (MQ=255)

                                            ttttAATAGCACCGCCTGCTTTCTAATCTACCACAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTg                                                                                            <  1:895892/140‑1 (MQ=255)

                                              ttAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGt                                                                                          >  1:851745/1‑140 (MQ=255)

                                                 aTAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCGCGCGTTAACCTCCAGTAGACCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAGACGTCCCCCTTGAGTCGTGGTATc                                                                                       >  2:818547/1‑140 (MQ=255)

                                                 aTAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATc                                                                                       >  1:3883726/1‑140 (MQ=255)

                                                     cACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGGCCTCCCTTTTCGATAGCGCCAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTc                                                                                   >  2:2190759/1‑140 (MQ=255)

                                                       ccGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGc                                                                                 >  1:4596594/1‑140 (MQ=255)

                                                        cGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCt                                                                                >  1:3258313/1‑140 (MQ=255)

                                                         gCCTGCTTTCTGATCTCCCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                                                               <  1:4177581/140‑1 (MQ=255)

                                                         gCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                                                               <  2:2493892/140‑1 (MQ=255)

                                                         gCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                                                               <  1:4288800/140‑1 (MQ=255)

                                                         gCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGACCGCCTTTAATCGTGGTATCATTCGCTg                                                                               <  2:4253732/140‑1 (MQ=255)

                                                          ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgc                                                                              >  1:4979754/1‑140 (MQ=255)

                                                          ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgc                                                                              >  1:1668214/1‑140 (MQ=255)

                                                          ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgc                                                                              >  1:1709999/1‑140 (MQ=255)

                                                          ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgc                                                                              >  2:2794205/1‑140 (MQ=255)

                                                           cTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgcg                                                                             >  1:3764525/1‑140 (MQ=255)

                                                                ttCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GG‑C‑CCCCGCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATa                                                                        >  1:4251774/1‑140 (MQ=255)

                                                                 tCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATaa                                                                       <  1:4105432/140‑1 (MQ=255)

                                                                 tCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATaa                                                                       <  2:727169/140‑1 (MQ=255)

                                                                        tACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTa                                                                >  1:2223606/1‑140 (MQ=255)

                                                                         cccGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                               <  2:4149048/139‑1 (MQ=255)

                                                                         aCCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                               <  1:1774804/140‑1 (MQ=255)

                                                                         aCCGCAAACGGAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGACACAAAGAC‑GGCC‑CCCC‑CATCTTGTTCAACGATTATGCGCCCGCCTTTAATCGTGGCATCATTCGCTGCGCCATAAACCTGTAg                                                               <  2:3734756/140‑1 (MQ=255)

                                                                          ccGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGc                                                              >  1:4455585/1‑140 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  2:3250833/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  2:3795602/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  2:2339041/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  1:1096554/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  1:3853103/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  1:2625711/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  2:1012772/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  1:5042353/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  1:1955941/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  1:4409226/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCATCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCAGAAACCTGTAGca                                                             <  2:4883223/140‑1 (MQ=255)

                                                                           cGCAAATGCAAAGCCCCCCTCTTTAACCTCCAGTCGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CCTCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  1:1655295/140‑1 (MQ=255)

                                                                           cGCAAATGCAAAGCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  2:1807368/140‑1 (MQ=255)

                                                                             caaatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGcaca                                                           <  1:1141117/140‑1 (MQ=255)

                                                                             caaatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGcaca                                                           <  2:202172/140‑1 (MQ=255)

                                                                              aaatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTACGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCacac                                                          <  2:1652238/140‑1 (MQ=255)

                                                                               aatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACAcc                                                         >  1:2396037/1‑140 (MQ=255)

                                                                                    aaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCattat                                                    <  2:1314670/140‑1 (MQ=255)

                                                                                    aaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCattat                                                    <  1:397715/140‑1 (MQ=255)

                                                                                     aatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCattatt                                                   <  2:42041/140‑1 (MQ=255)

                                                                                     aatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCattatt                                                   <  2:2691969/140‑1 (MQ=255)

                                                                                     aatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCattatt                                                   >  1:3135444/1‑140 (MQ=255)

                                                                                     aacccccccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAATCTGTAGCACACCattatt                                                   <  2:4481723/137‑1 (MQ=255)

                                                                                       tCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGa                                                 <  1:3199769/140‑1 (MQ=255)

                                                                                       tCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGa                                                 <  2:3315036/140‑1 (MQ=255)

                                                                                        ccccccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAg                                                <  1:3816067/140‑1 (MQ=255)

                                                                                        ccccccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTAGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAg                                                <  2:1239316/140‑1 (MQ=255)

                                                                                          ccccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCg                                              <  2:851745/140‑1 (MQ=255)

                                                                                           cccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGt                                             >  2:1849326/1‑140 (MQ=255)

                                                                                                  tAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTgg                                      >  2:126967/1‑140 (MQ=255)

                                                                                                  tAACCTCCAGTAGGCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGAGTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTgg                                      >  2:2210719/1‑140 (MQ=255)

                                                                                                          agtagtCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcgc                              >  1:2983270/1‑140 (MQ=255)

                                                                                                              gtCCTCCCTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCa                          <  1:682004/140‑1 (MQ=255)

                                                                                                                   cccTTTTCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCAt                     >  1:2011762/1‑140 (MQ=255)

                                                                                                                      ttttCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTg                  <  2:2723080/140‑1 (MQ=255)

                                                                                                                      ttttCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTg                  >  1:149441/1‑140 (MQ=255)

                                                                                                                        ttCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGaa                <  1:4594570/140‑1 (MQ=255)

                                                                                                                         tCGATAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAAc               <  1:4980063/140‑1 (MQ=255)

                                                                                                                             tAGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                                                               >  1:2863341/1‑72 (MQ=255)

                                                                                                                              aGCGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAAttt          >  1:1352026/1‑140 (MQ=255)

                                                                                                                                cGACAAAGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGa        >  1:3876657/1‑140 (MQ=255)

                                                                                                                                      aGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTTATAACCg  <  2:3456075/140‑1 (MQ=255)

                                                                                                                                      aGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  2:4369122/140‑1 (MQ=255)

                                                                                                                                      aGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:2031676/140‑1 (MQ=255)

                                                                                                                                      aGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  2:1704642/140‑1 (MQ=255)

                                                                                                                                      aGAC‑GGCC‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:1083128/140‑1 (MQ=255)

                                                                                                                                      aGAC‑GG‑CTCCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  2:3215696/140‑1 (MQ=255)

ACCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGAC‑GG‑C‑CCCC‑CATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCG  >  NZ_CP025268/2292451‑2292723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`