Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 2,466,923 (C)8→9 intergenic (+242/‑123) fadL → / → CXP41_RS12955 long‑chain fatty acid transporter FadL/protein YfdF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252682,466,9151.C94.8% 419.9 / 15.6 136intergenic (+234/‑131)fadL/CXP41_RS12955long‑chain fatty acid transporter FadL/protein YfdF
Reads supporting (aligned to +/- strand):  ref base . (0/7);  new base C (12/115);  total (12/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 171 total aligned reads displayed.

TTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAA  >  NZ_CP025268/2466778‑2467033
                                                                                                                                          |                                                                                                                          
ttGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑cc                                                                                                                         >  1:1019616/1‑140 (MQ=255)
ttGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑cc                                                                                                                         >  1:1019589/1‑140 (MQ=255)
 tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑cc‑c                                                                                                                       <  1:865367/140‑1 (MQ=255)
 tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑cc‑c                                                                                                                       <  2:2013432/140‑1 (MQ=255)
 tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑cc‑c                                                                                                                       <  2:2571955/140‑1 (MQ=255)
 tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑cc‑c                                                                                                                       <  2:2587901/140‑1 (MQ=255)
 tGCAAACAACATGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACCAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTCCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATCCT‑‑cc‑c                                                                                                                       <  2:3754949/140‑1 (MQ=255)
  ccaaccggCCTCTTGCCAGCTGCCCCTTTCAAATCAATAACCGGTACCCACAACCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCGGCCCGTCCTCCCCAAAGAGTTTACTTTTCTGCATTCCCAGGATAcc‑‑cc‑c‑c                                                                                                                     <  2:682656/133‑1 (MQ=255)
     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑cc‑c‑cc‑cc                                                                                                                 <  2:5093455/140‑1 (MQ=255)
     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑cc‑c‑cc‑cc                                                                                                                 <  1:3904351/140‑1 (MQ=255)
     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑cc‑c‑cc‑cc                                                                                                                 <  1:1247132/140‑1 (MQ=255)
     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑cc‑c‑cc‑cc                                                                                                                 <  1:2354048/140‑1 (MQ=255)
      aCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑cc‑c‑cc‑ccc                                                                                                                <  2:4892166/140‑1 (MQ=255)
       cAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAGACT‑‑cc‑c‑cc‑cccc                                                                                                               <  2:1383981/140‑2 (MQ=255)
       cAGCCTGTTGGCAGCTTGCCCTTTCAAATAAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAACGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑cc‑c‑cc‑cccc                                                                                                               <  2:170575/140‑2 (MQ=255)
       cAGCCTGTTGGCAGCTTGCCCTTCCAAATCAATAACCGGTATCCACAGCCAAACCTAAAGGGAAAGGCATTTTTTCAGGCGTTCTGCCCGTCCTTCCCAAAGAGTTTCCTTTTCTGCTTTCCCAGGATCCT‑‑cc‑c‑cc‑cccc                                                                                                               <  2:1727510/140‑2 (MQ=255)
        aGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑cc‑c‑cc‑cccct                                                                                                              <  2:1866859/140‑3 (MQ=255)
          ccTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATCCT‑‑cc‑c‑cc‑cccctgg                                                                                                            <  1:3419970/140‑5 (MQ=255)
          ccTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑cc‑c‑cc‑cccctgg                                                                                                            <  1:1889368/140‑5 (MQ=255)
          ccTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTAGCCCCAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑cc‑c‑cc‑cccctgg                                                                                                            <  2:282656/140‑5 (MQ=255)
            tGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCt                                                                                                           <  1:3311790/140‑1 (MQ=255)
              ttGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAATGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTAt                                                                                                         <  2:3841222/140‑1 (MQ=255)
                ggCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATtg                                                                                                       <  1:5065065/140‑1 (MQ=255)
                ggCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATtg                                                                                                       <  1:5064411/140‑1 (MQ=255)
                ggCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATtg                                                                                                       <  1:2707736/140‑1 (MQ=255)
                  cAGCTTGCCCTTTCAAATCAATCAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATtgtg                                                                                                     <  2:4629283/140‑1 (MQ=255)
                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATtgtg                                                                                                     <  1:1997654/140‑1 (MQ=255)
                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATtgtg                                                                                                     <  1:2755030/140‑1 (MQ=255)
                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATtgtg                                                                                                     <  1:2866661/140‑1 (MQ=255)
                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATtgtg                                                                                                     <  1:415405/140‑1 (MQ=255)
                    gcgtGCCCGTTCAAATCAATAAGCGGTATCCACAGACAAACCGCACGGGAAAGGCATATTTTCAGGCGTTCTGCTCGCCCTTCTCAAAAAGTTTCCTTGTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCGATTGTgcg                                                                                                   <  2:1567840/137‑1 (MQ=255)
                    gcctGCCCTTTCAAATCAATAACCGGTATCCCCAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTgcg                                                                                                   <  2:4936737/137‑1 (MQ=255)
                    gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTgcg                                                                                                   <  1:3322789/140‑1 (MQ=255)
                    gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTgcg                                                                                                   <  1:130597/140‑1 (MQ=255)
                       tGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTc                                                                                                <  2:2884167/140‑1 (MQ=255)
                         cccTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCAt                                                                                              <  2:3777425/140‑1 (MQ=255)
                          ccTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATa                                                                                             <  1:1043266/140‑1 (MQ=255)
                          ccTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATa                                                                                             <  2:3959975/140‑1 (MQ=255)
                          ccTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATa                                                                                             <  1:4331905/140‑1 (MQ=255)
                              tCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACt                                                                                         <  1:4687254/140‑1 (MQ=255)
                              tCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACt                                                                                         >  2:576049/1‑140 (MQ=255)
                              tCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACt                                                                                         <  1:2550974/140‑1 (MQ=255)
                               cAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTc                                                                                        <  1:4171006/140‑1 (MQ=255)
                               cAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTc                                                                                        <  2:3731709/140‑1 (MQ=255)
                                  aTCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCaaa                                                                                     <  1:3862068/140‑1 (MQ=255)
                                     aaTAAGCGGTATCCACAGACAAAGCTGAAGGGAAAGGCATCTTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTa                                                                                  <  1:4689952/140‑1 (MQ=255)
                                     aaTAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTa                                                                                  <  2:918650/140‑1 (MQ=255)
                                     aaTAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTa                                                                                  <  1:3835108/140‑1 (MQ=255)
                                          tcgttATCTACATACAAACCTGAAGGGAAAGGCATGTTTTCTGGCGTCCTGCTCGTCCTTTCCAAAGATGTTACTTTTCTTCATCGCCAGGATACT‑‑CCTC‑TC‑CCCTGGCCCTTGTGCGCCCATTCACTCAACTTaaattt                                                                             <  2:1829499/136‑4 (MQ=255)
                                          gaggTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTGTCAGGATACT‑‑CC‑CTCC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                             <  2:1838474/138‑1 (MQ=255)
                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATCTTTTCAGGCGTTCTGCCCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCCCATACACTCAAATTAAAGAt                                                                             <  2:4051320/140‑1 (MQ=255)
                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                             <  1:598570/140‑1 (MQ=255)
                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                             <  2:857960/140‑1 (MQ=255)
                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                             <  1:1330219/140‑1 (MQ=255)
                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                             <  1:2622590/140‑1 (MQ=255)
                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                             <  1:2956620/140‑1 (MQ=255)
                                           cGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                             <  1:3557708/140‑1 (MQ=255)
                                               aTCCACAGACACACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                        <  2:4313393/140‑1 (MQ=255)
                                               aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGTATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                        <  2:3399099/140‑1 (MQ=255)
                                               aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                        <  1:1108566/140‑1 (MQ=255)
                                               aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                        <  1:285531/140‑1 (MQ=255)
                                                        cAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaataaa                                                               >  1:1508521/1‑140 (MQ=255)
                                                            ccTGATGGGAAAGGGATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTCCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                           <  2:4503125/140‑1 (MQ=255)
                                                            ccTGAAGGTAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAATAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                           <  2:4539999/140‑1 (MQ=255)
                                                            ccTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                           >  2:3267810/1‑140 (MQ=255)
                                                               gAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGt                                                        >  1:3381841/1‑140 (MQ=255)
                                                                aaGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGtt                                                       <  2:4454303/140‑1 (MQ=255)
                                                                 aGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGttt                                                      >  1:2591335/1‑140 (MQ=255)
                                                                     aaaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTg                                                  <  2:3679353/140‑1 (MQ=255)
                                                                      aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                 <  2:695697/140‑1 (MQ=255)
                                                                      aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                 <  2:2278085/140‑1 (MQ=255)
                                                                      aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                 <  1:2231479/140‑1 (MQ=255)
                                                                      aaGGCATATTTTCAGGCGTTCTGCTCGTCCTGCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                 <  1:1620737/140‑1 (MQ=255)
                                                                        ggCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATa                                               <  1:1094564/140‑1 (MQ=255)
                                                                         gCATATTTTCAGGCCTTCTGCTCGTCCTTCTCAAAGAGCTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTACAGATAGGTTCTACATAAATGAGCGTTTTTTGATAg                                              <  1:194025/140‑1 (MQ=255)
                                                                          cATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGt                                             <  1:4403802/140‑1 (MQ=255)
                                                                          cATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGt                                             <  2:3257690/140‑1 (MQ=255)
                                                                            tatTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCt                                           <  1:3742955/140‑1 (MQ=255)
                                                                            tatTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa                                          <  1:3590984/140‑1 (MQ=255)
                                                                              ttttCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa                                          <  2:3918109/140‑1 (MQ=255)
                                                                                  cAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                     <  2:1019589/140‑1 (MQ=255)
                                                                                  cAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                     <  1:4692334/140‑1 (MQ=255)
                                                                                  cAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                     <  2:1019706/140‑1 (MQ=255)
                                                                                   aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                     >  1:1645013/1‑139 (MQ=255)
                                                                                   aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAt                                    >  2:4469707/1‑140 (MQ=255)
                                                                                    ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                   <  2:1407156/140‑1 (MQ=255)
                                                                                     gCGTTCTGCTCGTCCTTCTCAAATAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTa                                  <  2:4043554/140‑1 (MQ=255)
                                                                                     gCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTa                                  <  2:336767/140‑1 (MQ=255)
                                                                                     gCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTa                                  >  1:1306395/1‑140 (MQ=255)
                                                                                      cGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAg                                 <  1:704637/140‑1 (MQ=255)
                                                                                       gTTCTGCTCTCCTTTCTCAAAGAGTTTACTTTTCTTCATTTCCAGGATTCT‑‑CC‑C‑CCTCCCTGGCTATTGTGCGCTCATACACTCAAATTTAAGATAGGTTCTAAGTAAATGAGCGTTTTTTGACAGTCTATTTCATTAgg                                <  2:2768038/140‑1 (MQ=255)
                                                                                       gTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                <  1:2378611/140‑1 (MQ=255)
                                                                                       cttCCGCTCGTCCTTCCCCAAGAGTTTACTCTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                <  2:3914099/139‑1 (MQ=255)
                                                                                         tCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTa                              <  2:349439/140‑1 (MQ=255)
                                                                                         tCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTa                              <  2:3381841/140‑1 (MQ=255)
                                                                                         tCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTa                              <  2:4120039/140‑1 (MQ=255)
                                                                                             cTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                           <  1:2352268/107‑1 (MQ=255)
                                                                                             cTCGCCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCCAATCAAAGATAGGTTCTAAATAAATGCGCGTTTTTTGATAGTCTATTTCATTAGGTAatat                          <  2:2790068/140‑1 (MQ=255)
                                                                                                                     tttCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCaaaa  <  2:1618044/140‑1 (MQ=255)
                                                                                                                     tttCTGCATTTCCAGGATACTC‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCaaaa  <  2:3093161/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                          
TTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑‑CC‑C‑CC‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAA  >  NZ_CP025268/2466778‑2467033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑