Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 2,773,671 (C)5→6 intergenic (+161/‑55) yfjQ → / → yfjR DUF932 domain‑containing protein/WYL domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252682,773,6661.C94.6% 859.3 / 40.2 280intergenic (+156/‑60)yfjQ/yfjRDUF932 domain‑containing protein/WYL domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base . (14/1);  new base C (179/85);  total (193/87)
Fisher's exact test for biased strand distribution p-value = 4.31e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 313 total aligned reads displayed.

TTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑G‑CC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTG  >  NZ_CP025268/2773529‑2773801
                                                                                                                                            |                                                                                                                                         
  gttGATATCGGACACAACCTGTCCGAATCGCTATCTGCTCGTGTGACTCAATCCCCCGGTTATAGATTTTAACCCCCGGTACAGCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGGCCATACCCTGT‑CC‑G‑cc‑c‑c                                                                                                                                     <  2:459618/140‑1 (MQ=255)
   ttAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACCTCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑G‑cc‑c‑cc                                                                                                                                    <  2:3954965/140‑1 (MQ=255)
   ttAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAGAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑G‑cc‑c‑cc                                                                                                                                    <  2:2262894/140‑1 (MQ=255)
   ttAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑G‑cc‑c‑cc                                                                                                                                    <  1:1124619/140‑1 (MQ=255)
   ttAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑G‑cc‑c‑cc                                                                                                                                    <  1:4883437/140‑1 (MQ=255)
   ttAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑G‑cc‑c‑cc                                                                                                                                    <  1:2283137/140‑1 (MQ=255)
   ttAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTCCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑G‑cc‑c‑cc                                                                                                                                    <  2:2820147/140‑1 (MQ=255)
   ttAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTGTTGCAGAAATAAAAAATAGTTTCTGCTTTGGCCATACCCTGT‑CC‑G‑cc‑c‑cc                                                                                                                                    >  2:2470193/1‑140 (MQ=255)
     aaTATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑G‑cc‑c‑ccct                                                                                                                                  >  1:55899/1‑138 (MQ=255)
     aaTATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGCCCCTTGCAGAAATAAAAAATAGTTTCGGCGCTGTCCATCCCCTCT‑CC‑G‑cc‑c‑cccc                                                                                                                                  >  2:3687837/1‑138 (MQ=37)
       tatCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑G‑cc‑c‑ccctct                                                                                                                                <  1:4190939/140‑5 (MQ=255)
           ggACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAACCCCCCGGTTATCATCTTTAACCTCCGTTACGTCTGGCTTTTGCATAAATCAAAAATAGTTTCTCCGTTGTCGATTCGCTGT‑CC‑G‑CC‑CGCCTCttttc                                                                                                                             >  2:4797897/1‑138 (MQ=25)
            gACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCC‑A‑CC‑C‑CCTCTTTaaa                                                                                                                            >  1:885397/1‑140 (MQ=255)
             acacCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CG‑GCCC‑C‑CCTCTCTAAAg                                                                                                                           >  2:1313541/1‑140 (MQ=255)
                 caccTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTaaa                                                                                                                       >  1:3794052/1‑139 (MQ=255)
                 caccTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAAt                                                                                                                       >  2:2558837/1‑140 (MQ=255)
                      gTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCAcat                                                                                                                  <  1:2243820/140‑1 (MQ=255)
                      gTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCAcat                                                                                                                  <  1:4409608/140‑1 (MQ=255)
                       tCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTCAAGTAATCAcatc                                                                                                                 >  1:2044530/1‑140 (MQ=255)
                           cATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCAttt                                                                                                             >  2:4972555/1‑140 (MQ=255)
                              cGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTtca                                                                                                          <  1:694094/140‑1 (MQ=255)
                                 tATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCAcat                                                                                                                  >  1:4022742/1‑129 (MQ=255)
                                 tATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTtcagtc                                                                                                       >  1:1799666/1‑140 (MQ=255)
                                      gCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTa                                                                                                  >  1:4956211/1‑140 (MQ=255)
                                       cgcGTGTGCCCCAATCCCCCGTTCATAGCTTCTACCCCCCGTTACAGCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTaa                                                                                                 <  1:3381399/138‑1 (MQ=18)
                                        tCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAAc                                                                                                >  2:1965944/1‑140 (MQ=255)
                                        tCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCCGGCTTTTGCAGAAATAAAAACTAGTTTGTGCGTTGTCCATGCCCTGT‑CC‑G‑CCGC‑TCTCTTTAAAGTAATGACATCACTTTCAGTCAGTTAAc                                                                                                >  2:4005027/1‑140 (MQ=255)
                                          gtgtGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACtt                                                                                              >  1:1366398/1‑140 (MQ=255)
                                          gtgtGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACtt                                                                                              >  2:4349760/1‑140 (MQ=255)
                                           tgtgCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACttt                                                                                             >  2:4900359/1‑140 (MQ=255)
                                           tgtgCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACttt                                                                                             >  1:828252/1‑140 (MQ=255)
                                               ccTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCCGTTAACTTTCCTg                                                                                         >  1:2076769/1‑140 (MQ=255)
                                                  cAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTACATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGgag                                                                                      <  1:1196926/140‑1 (MQ=255)
                                                    aTCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGaa                                                                                    >  1:102755/1‑140 (MQ=255)
                                                    aTCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGaa                                                                                    <  2:1358520/140‑1 (MQ=255)
                                                    aTCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGaa                                                                                    <  1:4680289/140‑1 (MQ=255)
                                                         ccGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAtctct                                                                               >  1:3866146/1‑140 (MQ=255)
                                                          cGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAtctctc                                                                              <  1:1588811/140‑1 (MQ=255)
                                                            gTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCAt                                                                            >  2:3443799/1‑140 (MQ=255)
                                                             ttATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAAtctctc                                                                              >  2:535151/1‑137 (MQ=255)
                                                               ataGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGac                                                                         <  1:3677281/140‑1 (MQ=255)
                                                               ataGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGac                                                                         <  2:1662219/140‑1 (MQ=255)
                                                                      ttAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCa                                                                  >  2:4635561/1‑140 (MQ=255)
                                                                      ttAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCa                                                                  >  2:1199871/1‑140 (MQ=255)
                                                                      ttAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCa                                                                  >  2:4025022/1‑140 (MQ=255)
                                                                      ttAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCa                                                                  >  2:1771438/1‑140 (MQ=255)
                                                                      ttAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCa                                                                  >  1:3031365/1‑140 (MQ=255)
                                                                      ttAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCa                                                                  >  1:3189430/1‑140 (MQ=255)
                                                                       tAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAg                                                                 >  1:2370982/1‑140 (MQ=255)
                                                                         aCCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGaa                                                               <  1:3232649/140‑1 (MQ=255)
                                                                              cGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAAcgccg                                                          >  2:3765398/1‑140 (MQ=255)
                                                                              cGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAAcgccg                                                          >  1:2818911/1‑140 (MQ=255)
                                                                                      cTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGAcc                                                  <  1:3435173/140‑1 (MQ=255)
                                                                                        ggCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CCTC‑CC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACGTTCCTGGAGTATCTCTCATGACACATGCAGAACGCCGCCATGACCgg                                                >  2:505795/1‑140 (MQ=14)
                                                                                         gCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCggc                                               >  1:1500145/1‑140 (MQ=255)
                                                                                         gCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCggc                                               <  1:1953846/140‑1 (MQ=255)
                                                                                             ttGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCggctggc                                           >  1:4858642/1‑140 (MQ=255)
                                                                                                cAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTggctgt                                        <  2:3873453/140‑1 (MQ=255)
                                                                                                    aaTAAAACATCGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACGTCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACCCCGCCATGACCGGCTggctgtcagg                                    <  2:1414397/140‑1 (MQ=17)
                                                                                                    aaTAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTggctgtcagg                                    <  1:1152031/140‑1 (MQ=255)
                                                                                                    aaTAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTggctgtcagg                                    <  2:1402765/140‑1 (MQ=255)
                                                                                                    aaTAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTggctgtcagg                                    <  2:1588160/140‑1 (MQ=255)
                                                                                                    aaTAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTggctgtcagg                                    <  2:444559/140‑1 (MQ=255)
                                                                                                        aaaaaTAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTggctgtcaggctgt                                <  1:991337/140‑1 (MQ=255)
                                                                                                        aaaaaTAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTggctgtcaggctgt                                <  2:3104683/140‑1 (MQ=255)
                                                                                                           aaTAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCAc                             <  1:3456734/140‑1 (MQ=255)
                                                                                                            aTAGTTTCTGCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACt                            <  1:3543877/140‑1 (MQ=255)
                                                                                                                     gCGTTGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAg                   <  2:3177558/140‑1 (MQ=255)
                                                                                                                       gTTGTCCATACCCTGT‑CC‑G‑CC‑CACCTCGTTAAAGTAATCACATCATTTACAGTCAGTTAACTTTCCTGGAGAATCTCTCATGAGACAGGCAGAACGCCGCCATGAGCGGCTGGCTGTCAGGCTGTCACTCATACTCAGcc                 >  2:3752169/1‑140 (MQ=14)
                                                                                                                         tGTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGt               <  1:2775668/140‑1 (MQ=255)
                                                                                                                          gTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTc              >  1:639200/1‑140 (MQ=255)
                                                                                                                          gTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTc              >  1:2927216/1‑140 (MQ=255)
                                                                                                                          gTCCATACCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGAACGGCTGGCTGTCAGGCTGTCACGGAGAAGCAGCCGtt              >  2:658922/1‑139 (MQ=14)
                                                                                                                                aCCCTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCGGTCACTGATAATCAGCCGTCTGGTTg        <  1:4091431/140‑1 (MQ=255)
                                                                                                                                 cccTGT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGCCCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGc       <  2:2915983/140‑1 (MQ=255)
                                                                                                                                     gT‑CC‑GCCC‑C‑CCTCTTTAACGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCGCTCATGACACAGGCAGAACGCCGCCATGACCGGCGGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  1:1851515/1‑140 (MQ=14)
                                                                                                                                     gT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCATTTAACTTTCCTGGAGAATCTCTCATGACACAGTCAGAACGCCGCCATGACCGGCTGGCTGTCAGTCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  2:1330783/1‑140 (MQ=14)
                                                                                                                                     gT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTGAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  1:5038251/1‑140 (MQ=255)
                                                                                                                                     gT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGTGGGt   >  1:2567169/1‑140 (MQ=255)
                                                                                                                                     gT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCgggg   >  2:1518390/1‑139 (MQ=255)
                                                                                                                                     gT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCgggg   >  2:4201492/1‑139 (MQ=255)
                                                                                                                                     gT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  1:234451/1‑140 (MQ=255)
                                                                                                                                     gT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  2:4851244/1‑140 (MQ=255)
                                                                                                                                     gT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  1:2692928/1‑140 (MQ=255)
                                                                                                                                     gT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  1:1745328/1‑140 (MQ=255)
                                                                                                                                     gT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  1:3292541/1‑140 (MQ=255)
                                                                                                                                     gT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  1:4036058/1‑140 (MQ=255)
                                                                                                                                     gT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  1:4465161/1‑140 (MQ=255)
                                                                                                                                     gT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  2:2209659/1‑140 (MQ=255)
                                                                                                                                     gT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  1:4590018/1‑140 (MQ=255)
                                                                                                                                     gT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  1:51943/1‑140 (MQ=255)
                                                                                                                                     gT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  1:633309/1‑140 (MQ=255)
                                                                                                                                     gT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  2:3521435/1‑140 (MQ=255)
                                                                                                                                     gT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  2:3503059/1‑140 (MQ=255)
                                                                                                                                     gT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  2:1834185/1‑140 (MQ=255)
                                                                                                                                     gT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  2:2044660/1‑140 (MQ=255)
                                                                                                                                     gT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGCTGCGGGt   >  2:2727860/1‑140 (MQ=255)
                                                                                                                                     gT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGACTGGTTGCGGGt   >  2:2984472/1‑140 (MQ=255)
                                                                                                                                     gT‑CC‑GCCC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACACCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  1:4259958/1‑140 (MQ=255)
                                                                                                                                     gT‑CC‑GCCC‑C‑CCCCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt   >  1:1573827/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                         
TTGTTAATATCGGACACCACCTGTCCGCATCGCTATGTGCTCGTGTGCCTCAATCCCCCGGTTATAGCTTTTAACCCCCGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGT‑CC‑G‑CC‑C‑CCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTG  >  NZ_CP025268/2773529‑2773801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑