BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,786,415	+C	pseudogene (334/4580 nt)	ypjA ←	adhesin‑like autotransporter YpjA/EhaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,786,411	1	.	C	94.0%	1073.6 / 56.2	338	pseudogene (338/4580 nt)	ypjA	adhesin‑like autotransporter YpjA/EhaD
Reads supporting (aligned to +/- strand): ref base . (11/9); new base C (159/156); total (173/165)
Fisher's exact test for biased strand distribution p-value = 8.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 357 total aligned reads displayed.

GCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑A‑C‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACA  >  NZ_CP025268/2786273‑2786545

gCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCCCTCTCGATTGTCGTACCTGTGGAAAT‑A‑c                                                                                                                                         >  1:4431784/1‑140 (MQ=255)

gCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑A‑c                                                                                                                                         >  1:5022741/1‑140 (MQ=255)

gCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑A‑c                                                                                                                                         >  1:2361378/1‑140 (MQ=255)

  tACCACCATCAATGTGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑A‑c‑c‑c                                                                                                                                     <  1:2510353/140‑1 (MQ=255)

  tACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑A‑c‑c‑c                                                                                                                                     <  2:2323297/140‑1 (MQ=255)

  tACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑A‑c‑c‑c                                                                                                                                     <  2:4191915/140‑1 (MQ=255)

  tACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑A‑c‑c‑c                                                                                                                                     <  1:2188644/140‑1 (MQ=255)

    ccaccaTCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑A‑c‑c‑ccc                                                                                                                                   >  1:3818170/1‑139 (MQ=255)

      cccaTCAATGAGTATTCCATTCGCCCTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑A‑c‑c‑cccgt                                                                                                                                 <  2:4907532/139‑4 (MQ=255)

      accaTCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑A‑c‑c‑cccgt                                                                                                                                 <  2:2861057/140‑4 (MQ=255)

          tCAATGAGTGTTCGATCCGCACTCCCTCCCTCTGGGCGCGAAGCACCGCCGTTACTCGGCGTTCCATTGCTGATACCCCCCTGATAACCGCCCTGGTTGCCCCTCTCGATTGTCGTGCCTGTGGAGAC‑AGC‑C‑CCGTCATg                                                                                                                              >  2:4557862/1‑140 (MQ=255)

          tCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATg                                                                                                                              >  1:4539644/1‑140 (MQ=255)

          tCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATg                                                                                                                              >  1:3787879/1‑140 (MQ=255)

          tCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAACTGTCGTTCCATTGCTGATGCCCCCTTTATAAAAGTGCTGATTGCCACTCTCGATTGTCGTACCTGTGTAAAT‑A‑CTC‑CCGGCATg                                                                                                                              >  2:184596/1‑140 (MQ=255)

              tGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTCATTGTCGTTCCCTTGCTGATACCCCCTTTATAACCGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATCC‑C‑C‑CCGTCATGAAtt                                                                                                                          <  1:3155268/140‑1 (MQ=255)

                  tATTCCATTCGCACTCCCTCCCTCTACTCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTGCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCCTAAATTGACt                                                                                                                      >  2:3755817/1‑140 (MQ=255)

                  tATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACt                                                                                                                      >  1:2319014/1‑140 (MQ=255)

                  tATTCCATTCGAACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACt                                                                                                                      <  2:799267/140‑1 (MQ=255)

                     tCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCTGCCTTAATTGTTGTTCGATTGCTGATACTAGCTTTATAAACGGCGTGATTGAGAGTATCGATTGTCGTACCTGTGGAAAT‑G‑C‑CGCTGTGGTTAATTGACTGtt                                                                                                                   >  2:3194946/1‑139 (MQ=255)

                        aTTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGc                                                                                                                <  1:4451733/140‑1 (MQ=255)

                          tCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCCATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCa                                                                                                              <  1:3597436/140‑1 (MQ=255)

                            gCACTCCCTCCCTCTACGCGTGAAGCACTGCCCTTAAGTGTTGGTCCATTGCTGATACCTCCTTTATAAACGTCCTGATTGCCACTCTCGATTTTCGTACCTTTGGAAAT‑A‑CTC‑CCGTCATGTATGGAATGTCTgtttcc                                                                                                            >  2:158830/1‑135 (MQ=255)

                            gCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCAcc                                                                                                            >  1:3527868/1‑140 (MQ=255)

                            gCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCAcc                                                                                                            >  1:4169515/1‑140 (MQ=255)

                               ctacctccctcTACGAGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGCACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGtt                                                                                                         <  2:4119799/137‑1 (MQ=255)

                                 ccctccctCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                                       <  2:3551069/140‑1 (MQ=255)

                                 ccctccctCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                                       <  1:1268055/140‑1 (MQ=255)

                                 ccctccctCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                                       <  2:4720363/140‑1 (MQ=255)

                                 ccctccctCTACGCGGGAAGCACCGCCCTTAAGTGTCGTTCCATTGCTGATACCCCCGTTACAAACGTCCTGATTGCCACTCTCGATTGTGGTACCTGTGGAAGT‑ACC‑C‑CCGTGATGAATTGACTGTCTGCCACCGTTaa                                                                                                       <  2:2538180/140‑1 (MQ=255)

                                   ctccctcTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCCTCATGAATTGACTGTCTGCCACCGTTAATg                                                                                                     <  1:3071018/140‑1 (MQ=255)

                                      cctcTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGtt                                                                                                  <  1:4331110/140‑1 (MQ=255)

                                       ctctACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTCTAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                                 <  2:427241/140‑1 (MQ=255)

                                       ctctACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                                                 <  1:685114/140‑1 (MQ=255)

                                          tACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGtat                                                                                              >  2:24324/1‑140 (MQ=255)

                                            cgcgTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGtatta                                                                                            >  1:785035/1‑140 (MQ=255)

                                            cgcgTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGtatta                                                                                            <  2:3678137/140‑1 (MQ=255)

                                              cgTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTattatt                                                                                          >  2:2913116/1‑140 (MQ=255)

                                              cgTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTattatt                                                                                          >  2:4039777/1‑140 (MQ=255)

                                                tGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAg                                                                                        >  2:4987327/1‑140 (MQ=255)

                                                 gAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGc                                                                                       <  2:3901066/140‑1 (MQ=255)

                                                  aaGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGcc                                                                                      >  1:84585/1‑140 (MQ=255)

                                                  aaGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGcc                                                                                      >  1:4819287/1‑140 (MQ=255)

                                                      aCCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGcc                                                                                  <  2:2128610/140‑1 (MQ=255)

                                                        cGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGcccc                                                                                >  2:1131273/1‑140 (MQ=255)

                                                         gCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCca                                                                               <  2:1176990/140‑1 (MQ=255)

                                                         gCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCca                                                                               <  1:3436155/140‑1 (MQ=255)

                                                          cccTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCTACCGGTAGTGGTTGTATTATTAGCCTGCCCcac                                                                              >  1:653419/1‑140 (MQ=255)

                                                          cccTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCcac                                                                              >  1:3558725/1‑140 (MQ=255)

                                                             ttAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACaaa                                                                           <  2:1454578/140‑1 (MQ=255)

                                                                   gTCGTTCCATTGCCGATACCCCCTTTATAACCGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGtt                                                                     <  2:2719321/140‑1 (MQ=255)

                                                                    tCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTa                                                                    >  2:2255253/1‑140 (MQ=255)

                                                                     cGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAATATTGTTAt                                                                   >  1:867656/1‑140 (MQ=255)

                                                                     cGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTAt                                                                   >  1:3276451/1‑140 (MQ=255)

                                                                      gTTCCATTGCTGATCCCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTCCCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATg                                                                  <  2:1227062/140‑1 (MQ=255)

                                                                       ttCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGa                                                                 <  1:4970929/140‑1 (MQ=255)

                                                                        tCCCTTGCTGCTACCCCCTTTATAGACGTCCTGATTGCCGCTCTCGCTTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGAc                                                                <  2:442542/140‑1 (MQ=255)

                                                                        tCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCCCTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGAc                                                                <  1:989256/140‑1 (MQ=255)

                                                                              gCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCta                                                          <  2:1013222/140‑1 (MQ=255)

                                                                                 gATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGAGTGTCGTGCCGGTGGAAAT‑A‑CAA‑GCGTCATGAATTGACTGTCTGGCAGGGTTAATGGTTGTATTATTAGCATGCACCAGAAAGTGGTTATGACTTCGTatat                                                       >  2:4490525/1‑140 (MQ=255)

                                                                                  aTACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                                       >  1:4646955/1‑91 (MQ=255)

                                                                                  aTACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATc                                                      >  1:1195311/1‑140 (MQ=255)

                                                                                  aTACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATc                                                      >  2:2217908/1‑140 (MQ=255)

                                                                                    aCCCCCTTTATAAGCGTCCTGAGTGCCACTCTCGAGTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGGCTGCCACCGTTAGTGGTTGTATTATTAGCCGGCCCCACAAAATTGTTATGACTTCCTATATCtt                                                    >  2:653907/1‑140 (MQ=255)

                                                                                    aCCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCtt                                                    >  1:1459123/1‑140 (MQ=255)

                                                                                    aCCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCtt                                                    >  1:2345986/1‑140 (MQ=255)

                                                                                    aCCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCtt                                                    <  1:2924065/140‑1 (MQ=255)

                                                                                    aCCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCtt                                                    >  1:3378405/1‑140 (MQ=255)

                                                                                         cTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATGTTGatat                                               >  1:2765808/1‑140 (MQ=255)

                                                                                         cTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGatat                                               >  2:87997/1‑140 (MQ=255)

                                                                                         cTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGatat                                               >  1:756182/1‑140 (MQ=255)

                                                                                         cTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGatat                                               >  1:1893647/1‑140 (MQ=255)

                                                                                         cTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGatat                                               >  2:4389971/1‑140 (MQ=255)

                                                                                            tataAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATcca                                            <  1:5063787/140‑1 (MQ=255)

                                                                                                aaCGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAg                                        >  1:1780624/1‑140 (MQ=255)

                                                                                                 aCGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGa                                       <  1:3651919/140‑1 (MQ=255)

                                                                                                   gTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGAtt                                     >  2:3297294/1‑140 (MQ=255)

                                                                                                    tCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTTTTATGACTTCCTATATCTTGATATCCACCAGATTc                                    >  1:3011583/1‑140 (MQ=255)

                                                                                                    tCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTc                                    >  2:1968045/1‑140 (MQ=255)

                                                                                                    tCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTc                                    >  1:4725153/1‑140 (MQ=255)

                                                                                                            gCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGAc                            <  2:3589107/140‑1 (MQ=255)

                                                                                                            gCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGAc                            <  1:3969215/140‑1 (MQ=255)

                                                                                                            gCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGAc                            <  1:2639604/140‑1 (MQ=255)

                                                                                                            gCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGAc                            <  1:4589633/140‑1 (MQ=255)

                                                                                                            gCCACTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATAATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGAc                            <  1:4049006/140‑1 (MQ=255)

                                                                                                               aCTCTCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTc                         >  2:260762/1‑140 (MQ=255)

                                                                                                                ctctCGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTcc                        <  2:4613108/140‑1 (MQ=255)

                                                                                                                 tctcGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTg                                                   >  1:1410167/1‑112 (MQ=255)

                                                                                                                 tctcGATTGTCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCa                       <  2:1375987/140‑1 (MQ=255)

                                                                                                                          tCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATAcc              <  1:4201117/140‑1 (MQ=255)

                                                                                                                          tCGTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATAcc              <  1:109819/140‑1 (MQ=255)

                                                                                                                            gTACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATAcccc            >  1:1557357/1‑140 (MQ=255)

                                                                                                                             tACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCg           >  1:2077780/1‑140 (MQ=255)

                                                                                                                             tACCTGTGGAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCg           >  2:5038258/1‑140 (MQ=255)

                                                                                                                                    ggAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGaa    >  2:1644930/1‑140 (MQ=255)

                                                                                                                                    ggAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGaa    >  1:196112/1‑140 (MQ=255)

                                                                                                                                    ggAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGaa    >  1:390377/1‑140 (MQ=255)

                                                                                                                                     gAAAT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAAc   >  2:1814661/1‑140 (MQ=255)

                                                                                                                                      aaaT‑ACC‑C‑CCGTCATTAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACa  <  2:3407403/140‑1 (MQ=255)

                                                                                                                                      aaaT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACa  <  1:1433428/140‑1 (MQ=255)

                                                                                                                                      aaaT‑ACC‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACa  <  2:2960141/140‑1 (MQ=255)

GCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAAT‑A‑C‑C‑CCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTAGATACCCCGCCATGAACA  >  NZ_CP025268/2786273‑2786545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`