BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,858,752	+T	pseudogene (395/2078 nt)	flhA →	formate hydrogenlyase transcriptional activator FlhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,858,748	1	.	T	97.4%	995.1 / 13.9	266	pseudogene (391/2078 nt)	flhA	formate hydrogenlyase transcriptional activator FlhA
Reads supporting (aligned to +/- strand): ref base . (4/3); new base T (117/142); total (121/145)
Fisher's exact test for biased strand distribution p-value = 7.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 305 total aligned reads displayed.

GCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑A‑TT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATGTC  >  NZ_CP025268/2858611‑2858881

gcagcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCTACTGGCGGCGGAAGGGCAT‑A‑tt                                                                                                                                       >  2:2837691/1‑140 (MQ=255)

  agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGTCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑A‑tt‑t‑t                                                                                                                                   >  1:2853105/1‑140 (MQ=255)

  agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑A‑tt‑t‑t                                                                                                                                   >  2:3689689/1‑140 (MQ=255)

  agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑A‑tt‑t‑t                                                                                                                                   >  2:1715331/1‑140 (MQ=255)

  agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑A‑tt‑t‑t                                                                                                                                   >  1:3472897/1‑140 (MQ=255)

  agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑A‑tt‑t‑t                                                                                                                                   >  1:4187305/1‑140 (MQ=255)

  agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑A‑tt‑t‑t                                                                                                                                   >  2:2468979/1‑140 (MQ=255)

  agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑A‑tt‑t‑t                                                                                                                                   >  1:2308713/1‑140 (MQ=255)

  agcaTTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑A‑tt‑t‑t                                                                                                                                   >  1:613777/1‑140 (MQ=255)

  agcaTTTTATCGCGCTCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAAGCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTGGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑A‑tt‑t‑t                                                                                                                                   >  2:2952704/1‑140 (MQ=255)

         tGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGc                                                                                                                             >  2:1420430/1‑140 (MQ=255)

           tCGCGCCCTGATACGCTGCATTGCTGTTACGAAGCATTTTCTGAAACCTCGCCGCAGCTGCACGCAGGTGTGCTATACCCTGAATTTGGTCACTATTGCCTAACGCCACTGGTGGCGGAAGGGCAT‑ATTT‑T‑TGGGGGCtg                                                                                                                           <  2:344660/140‑1 (MQ=255)

           tCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCtg                                                                                                                           <  1:2668060/140‑1 (MQ=255)

              cgcCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGa                                                                                                                        >  1:2607955/1‑140 (MQ=255)

              cgcCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGa                                                                                                                        >  1:30387/1‑140 (MQ=255)

                cccTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAAt                                                                                                                      >  1:1196122/1‑140 (MQ=255)

                   tGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTa                                                                                                                   <  1:4108806/140‑1 (MQ=255)

                    gATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCGGCAGCTGGACGCAGGTGAGCTACACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTAt                                                                                                                  <  2:3566139/140‑1 (MQ=255)

                    gATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTAt                                                                                                                  <  2:4813661/140‑1 (MQ=255)

                    gATACGCTGAACTGCAGTTACAAAGACTTTTGTGAACCCTGGAAGCAGCTGGACGCCGGTGGGCTATACCCAACATTTGGTCACTATTGCCTGATGCCACTGGCGGAGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTAt                                                                                                                  <  1:422968/140‑1 (MQ=255)

                         gCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGtt                                                                                                             <  1:1761817/140‑1 (MQ=255)

                           tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGTGCTATACCCAAAATTTGGTCACTATTGCCTGATTCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTGGTTAt                                                                                                           >  2:1845500/1‑140 (MQ=255)

                           tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTAt                                                                                                           >  1:289716/1‑140 (MQ=255)

                           tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTAt                                                                                                           >  2:3262651/1‑140 (MQ=255)

                           tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTAt                                                                                                           >  2:2380587/1‑140 (MQ=255)

                           tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTAt                                                                                                           >  1:2417973/1‑140 (MQ=255)

                           tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTAt                                                                                                           <  1:772291/140‑1 (MQ=255)

                           tGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTAt                                                                                                           >  1:4566641/1‑140 (MQ=255)

                             cATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGTCGGAAGGGCAT‑ATTT‑T‑TCGTGGCTGTGAATTTATTCGTTATGa                                                                                                         >  1:4653810/1‑140 (MQ=255)

                             cATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGa                                                                                                         >  2:4208846/1‑140 (MQ=255)

                             cATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGa                                                                                                         >  2:3112861/1‑140 (MQ=255)

                             cATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACCCAGGTGGGTTATAGTCTAAACTTGGGCACTATTGCCTGATTCCACTGGCGGCGGGAGGGCAT‑ATTT‑T‑TGGTGTCTGTGAACTTATTAGTTATgc                                                                                                         >  2:2549632/1‑139 (MQ=255)

                                 tcAGTTACGGAGAATTTTGTGAAACCTGGAGGCAGCTGGACGCAGGTGGGCTATACCTAAAAGTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGAAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                     <  2:3004275/139‑1 (MQ=255)

                                 gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                     <  2:4075127/140‑1 (MQ=255)

                                 gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                     <  2:2639971/140‑1 (MQ=255)

                                 gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                     <  2:3942445/140‑1 (MQ=255)

                                 gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                     <  1:1117204/140‑1 (MQ=255)

                                 gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                     <  1:1309456/140‑1 (MQ=255)

                                 gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                     <  1:2148576/140‑1 (MQ=255)

                                 gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGAt                                                                                                     <  1:3195161/140‑1 (MQ=255)

                                 gCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGGGGCTGCGAATTTATTCGTTATGACGAt                                                                                                     <  2:1189181/140‑1 (MQ=255)

                                     ttACGAAGAATTTTGTGAAACCTGGACGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGcc                                                                                                 >  2:4587439/1‑140 (MQ=255)

                                       aCGAAGCATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGcccc                                                                                               >  1:2244817/1‑140 (MQ=255)

                                       aCGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTTAATTTATTCGTTATGACGATCGcccc                                                                                               >  2:293668/1‑140 (MQ=255)

                                       aCGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGcccc                                                                                               >  2:506013/1‑140 (MQ=255)

                                       aCGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGcccc                                                                                               >  1:1606995/1‑140 (MQ=255)

                                       aCGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGcccc                                                                                               >  1:1429944/1‑140 (MQ=255)

                                       aCGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGcccc                                                                                               >  2:3788489/1‑140 (MQ=255)

                                           agaaTTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGa                                                                                           <  2:744652/140‑1 (MQ=255)

                                               ttttGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGa                                                                                       >  1:2019846/1‑140 (MQ=255)

                                                       aaCCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGt                                                                               >  1:125947/1‑140 (MQ=255)

                                                         ccTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTc                                                                             >  1:1561964/1‑140 (MQ=255)

                                                          ctggacgcagctggacgcagGTGAGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑A‑TTCT‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCa                                                                            <  2:4878357/140‑1 (MQ=255)

                                                            ggCCGCAGCTGGACGCAGGTGGGCTATACCCACAATTTGGTCACTATTGACTGATGCCACAGACGGCCGAAAGACAT‑G‑TT‑TCTGGTGGCTGAGCATTTAATGCTTGGGACGATCGCCCCGGGGGCGAACAAGAAttaacg                                                                          >  2:795068/1‑136 (MQ=255)

                                                             gCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATc                                                                         <  2:1037471/140‑1 (MQ=255)

                                                             gCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATc                                                                         <  1:3601496/140‑1 (MQ=255)

                                                             gCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATc                                                                         <  1:1171910/140‑1 (MQ=255)

                                                                  aGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTg                                                                    >  2:4705886/1‑140 (MQ=255)

                                                                    cTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCa                                                                  <  2:1519979/140‑1 (MQ=255)

                                                                    cTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCa                                                                  <  1:3467294/140‑1 (MQ=255)

                                                                          gCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACAtt                                                            <  1:1704875/140‑1 (MQ=255)

                                                                              gTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACg                                                        <  1:3314382/140‑1 (MQ=255)

                                                                              gTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACg                                                        <  1:3120623/140‑1 (MQ=255)

                                                                                 ggCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAg                                                     <  2:2240499/140‑1 (MQ=255)

                                                                                   cTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGAt                                                   <  2:3877624/140‑1 (MQ=255)

                                                                                   cTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGAt                                                   <  2:2141385/140‑1 (MQ=255)

                                                                                   cTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGAt                                                   <  2:2908948/140‑1 (MQ=255)

                                                                                      taCCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGt                                                >  2:2281201/1‑140 (MQ=255)

                                                                                      taCCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGt                                                >  2:4988367/1‑140 (MQ=255)

                                                                                        cccAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGttt                                              >  1:608143/1‑140 (MQ=255)

                                                                                        cccAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGttt                                              >  2:28638/1‑140 (MQ=255)

                                                                                         ccAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTc                                             >  1:5066112/1‑140 (MQ=255)

                                                                                           aaaaTTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTg                                           >  2:804117/1‑140 (MQ=255)

                                                                                             aaTTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTgtc                                         >  1:912561/1‑140 (MQ=255)

                                                                                             aaTTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTgtc                                         <  2:1976302/140‑1 (MQ=255)

                                                                                                  ggTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCAc                                    <  1:3689689/140‑1 (MQ=255)

                                                                                                   gTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCAcc                                   >  1:212854/1‑140 (MQ=255)

                                                                                                       cTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTCCGCAGATCGTTTCTGTCGTCACCGAAc                               <  2:3762440/140‑1 (MQ=255)

                                                                                                       cTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                               <  1:2493461/140‑1 (MQ=255)

                                                                                                       cTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                               <  2:91845/140‑1 (MQ=255)

                                                                                                       cTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                               <  1:3390053/140‑1 (MQ=255)

                                                                                                       cTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                               <  1:1383931/140‑1 (MQ=255)

                                                                                                       cTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                               <  1:1061802/140‑1 (MQ=255)

                                                                                                       cTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAAc                               <  1:3867048/140‑1 (MQ=255)

                                                                                                            gCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATc                          <  2:1276747/140‑1 (MQ=255)

                                                                                                            gCCTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATc                          <  1:798407/140‑1 (MQ=255)

                                                                                                             ccTGATGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGGGGCTGTGAATTTATTCGGTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTGTGCAAACATTTACGCAGATCGTTTCGGGCGTCACCGAACAAATcc                         >  2:4427395/1‑140 (MQ=255)

                                                                                                                  tGCCACTGGCGGCGGAAGGGCATGT‑TT‑T‑TGGTGGCTGTGAAGTTATTCGTTATGGCGATCTCCGCTGGGGCGAAACCGGGATCAATCGTCTGCAAACATTTACGCAGATCGGTTCTGTCGTCACCGCACATATCCAGAGc                    >  2:4375724/1‑140 (MQ=255)

                                                                                                                  tGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGc                    >  2:3335374/1‑140 (MQ=255)

                                                                                                                  tGCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TCGTGGCTGTGAATTTCTTCGTTGTGACGATCGCGACTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATAGTTTATGTCGTCACAGAACAAATACAGAGc                    >  2:4480195/1‑140 (MQ=255)

                                                                                                                  tGCCACTGGCGGAGGAAGGGCAT‑ATTT‑T‑TGGTTGGTGTGAATTTATTGGTTATGACGATGGCGCCTGGTGGGAAAAAGAGTTCAATGGGGTGCAAAGCGTTACGCAGATCGTTTCTGTCGTCACCGCGCGAATCCAGAGc                    >  2:1467412/1‑140 (MQ=255)

                                                                                                                   gCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGcc                   <  1:4382079/140‑1 (MQ=255)

                                                                                                                   gCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGcc                   <  1:381899/140‑1 (MQ=255)

                                                                                                                   gCCACTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGcc                   <  1:3788489/140‑1 (MQ=255)

                                                                                                                    ccACTGGCGGCGTAAGGGCAT‑ATTT‑T‑TGGTGGCTCTGAATTTAGCAGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCCATCGTCTGCAAACATTTACGCCGATCGTTTCTGTCGTCACCGCACAAATCCAGAGCcg                  <  2:3155613/140‑1 (MQ=255)

                                                                                                                      aCTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCcgcg                <  2:1625043/140‑1 (MQ=255)

                                                                                                                       cTGGCGGCGGAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGcgt               >  1:2049453/1‑140 (MQ=255)

                                                                                                                            ggcggAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTa          <  1:5001827/140‑1 (MQ=255)

                                                                                                                              cggAAGGGCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAAc        <  1:4330624/140‑1 (MQ=255)

                                                                                                                                   gtgCAT‑ATTT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACTATCGCCCCTGGAGCGAAAAAGATTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGCGCCGCGTCGTTAACAATGt   <  2:1098663/138‑1 (MQ=255)

GCAGCATTTTGTCGCGCCCTGATACGCTGCATTGCAGTTACGAAGAATTTTGTGAAACCTGGCCGCAGCTGGACGCAGGTGGGCTATACCCAAAATTTGGTCACTATTGCCTGATGCCACTGGCGGCGGAAGGGCAT‑A‑TT‑T‑TGGTGGCTGTGAATTTATTCGTTATGACGATCGCCCCTGGAGCGAAAAAGAGTTCAATCGTCTGCAAACATTTACGCAGATCGTTTCTGTCGTCACCGAACAAATCCAGAGCCGCGTCGTTAACAATGTC  >  NZ_CP025268/2858611‑2858881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`