BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	320,615	(G)_6→7	intergenic (+482/‑44)	rclR → / → ykgE	reactive chlorine‑specific transcriptional regulator RclR/heterodisulfide reductase‑related iron‑sulfur binding cluster

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	320,609	1	.	G	96.2%	672.9 / 18.2	212	intergenic (+476/‑50)	rclR/ykgE	reactive chlorine‑specific transcriptional regulator RclR/heterodisulfide reductase‑related iron‑sulfur binding cluster
Reads supporting (aligned to +/- strand): ref base . (5/3); new base G (125/79); total (130/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 218 total aligned reads displayed.

GCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAAC  >  NZ_CP025268/320471‑320743

 cGATGCCGAAAGAAATAAAATTAGTCATTGCATTTGACGTTTGCATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑gg                                                                                                                                        <  1:4657758/140‑1 (MQ=255)

            gaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACAt                                                                                                                            <  2:930307/140‑1 (MQ=255)

             aaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATc                                                                                                                           <  1:3980327/140‑1 (MQ=255)

               aTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCgg                                                                                                                         >  2:4864443/1‑140 (MQ=255)

                  aaaTTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTAATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCa                                                                                                                      <  2:4530961/140‑1 (MQ=255)

                      tagtatttGCATTTGACGTTTGGATAAAAGAGTATCCTGTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTCTTGATGATTGACCCCCCGGTGGGGGG‑G‑AAACATCGGGCATaaa                                                                                                                  <  2:159050/134‑1 (MQ=255)

                       aGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCAATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAAt                                                                                                                 >  2:95399/1‑140 (MQ=255)

                            ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCa                                                                                                            <  1:1117786/140‑1 (MQ=255)

                            ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCa                                                                                                            <  2:64680/140‑1 (MQ=255)

                            ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCa                                                                                                            <  1:1955942/140‑1 (MQ=255)

                              gCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATg                                                                                                          <  2:3290172/140‑1 (MQ=255)

                              gCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATg                                                                                                          <  2:2307301/140‑1 (MQ=255)

                              gCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATg                                                                                                          <  1:1793238/140‑1 (MQ=255)

                              gCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATg                                                                                                          <  2:3970686/140‑1 (MQ=255)

                              gCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATg                                                                                                          <  1:3278754/140‑1 (MQ=255)

                              gCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATg                                                                                                          <  2:2036146/140‑1 (MQ=255)

                              gCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGACGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATg                                                                                                          <  2:4506409/140‑1 (MQ=255)

                                 tttGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAg                                                                                                       >  1:4909629/1‑140 (MQ=255)

                                  ttGACGTTTGGATGAAAGATTATCAGTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGt                                                                                                      >  2:4581115/1‑140 (MQ=255)

                                   tggcGTTTGGATGGAAGATTATGATTTGTCAAACAAATGCGGGGAGGTATGTGGCTAGCCAATTACAGTAGAACGCCATATTTATTGATGATCGATCCTACGCT‑GGGGGTG‑TAACTTCGGGCATAAATGGGCATGAAGTa                                                                                                     >  2:1836305/4‑140 (MQ=255)

                                   tGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTa                                                                                                     <  1:3126483/140‑1 (MQ=255)

                                   tGACGTTTGGATGAAAGATTATCATTTGTCATACAAAGGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTa                                                                                                     >  2:1836136/1‑140 (MQ=255)

                                    gACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTaa                                                                                                    <  2:4331726/140‑1 (MQ=255)

                                    gACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTaa                                                                                                    <  1:3033350/140‑1 (MQ=255)

                                     aCGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAAt                                                                                                   <  2:3839970/140‑1 (MQ=255)

                                     aCGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAAt                                                                                                   <  1:1531577/140‑1 (MQ=255)

                                       gTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGCTTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATgg                                                                                                 <  2:4776629/140‑1 (MQ=255)

                                       gTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATgg                                                                                                 <  1:1205689/140‑1 (MQ=255)

                                         ttGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AGACATCGGGCATAAATGGGCATGAAGTAATGGAg                                                                                               >  1:1190760/1‑140 (MQ=255)

                                         ttGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAg                                                                                               >  1:3813314/1‑140 (MQ=255)

                                         ttGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGAATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAg                                                                                               >  1:646932/1‑140 (MQ=255)

                                          tGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTGGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATTATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAGGTAATGGAgg                                                                                              >  2:1729291/1‑139 (MQ=255)

                                                aaaGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGACAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑CAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGt                                                                                        >  1:3554886/1‑140 (MQ=255)

                                                     ttATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGa                                                                                   <  1:3529755/140‑1 (MQ=255)

                                                           tttGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCa                                                                             >  1:549448/1‑140 (MQ=255)

                                                             tGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATGAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAt                                                                           >  2:1552814/1‑140 (MQ=255)

                                                              gTCATACAAATGAGGGGTGGTATGTTGATAGCCAATTAAAAAAGAACGACATATTTATTGATGATTGATCCCCCGGT‑GGGGG‑GCAAACATCGGGCATAAATGGGCATGAAGTAATGGAATATTAGTTGTTAGTGTCAAtt                                                                          >  2:3742260/1‑140 (MQ=255)

                                                               tCATACAACTGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTGATTGGCGCTTGATCCCCCGGTGGGGGG‑G‑ACACATCGGGCATAAATGGGCATGGAGTAATGGAGGATAAGTTGTCGCGGTCAAttt                                                                         >  2:3156945/1‑140 (MQ=255)

                                                                  tACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGGTCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCtt                                                                      >  1:1989661/1‑140 (MQ=255)

                                                                  tACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATg                                                                                                          <  2:1228250/104‑1 (MQ=255)

                                                                  tACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATg                                                                                                          >  1:1228250/1‑104 (MQ=255)

                                                                   ataaaTGATGGGTGGTGTGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCttt                                                                     <  2:2448114/138‑1 (MQ=255)

                                                                   aCAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCttt                                                                     <  1:2251001/140‑1 (MQ=255)

                                                                     aaaTGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGt                                                                   <  1:739150/140‑1 (MQ=255)

                                                                     aaaTGAGGGGTGGTATCTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATTATTGAACCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATTTCAATTTCTTTGt                                                                   <  2:1986610/140‑1 (MQ=255)

                                                                      aaTGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTc                                                                  <  2:2697014/140‑1 (MQ=255)

                                                                        tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  1:2643889/140‑1 (MQ=255)

                                                                        tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  2:1122140/140‑1 (MQ=255)

                                                                        tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  1:4414036/140‑1 (MQ=255)

                                                                        tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  2:2088124/140‑1 (MQ=255)

                                                                        tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  1:555388/140‑1 (MQ=255)

                                                                        tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  2:3537027/140‑1 (MQ=255)

                                                                          aGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCt                                                              <  1:1675475/140‑1 (MQ=255)

                                                                            gggTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGt                                                            >  1:250560/1‑140 (MQ=255)

                                                                            gggTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGt                                                            >  1:1452417/1‑140 (MQ=255)

                                                                            gggTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGt                                                            >  2:3454835/1‑140 (MQ=255)

                                                                                 gtATGTTGCTAGCCAATTAAAAAGGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGTGCATAAATGGGCATGAAGTAAGGGAGTATTAGTTGTTAAGGTCAATCTCTTTGTCACCTGCATTgg                                                       >  2:3950905/1‑140 (MQ=255)

                                                                                 gtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTgg                                                       >  2:506415/1‑140 (MQ=255)

                                                                                 gtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTgg                                                       >  1:1051557/1‑140 (MQ=255)

                                                                                 gtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTgg                                                       >  2:1672447/1‑140 (MQ=255)

                                                                                 gtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTgg                                                       >  1:3594469/1‑140 (MQ=255)

                                                                                 gtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTgg                                                       >  1:4930012/1‑140 (MQ=255)

                                                                                 gtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTgg                                                       >  1:3840257/1‑140 (MQ=255)

                                                                                       tGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTc                                                                  <  1:938368/123‑1 (MQ=255)

                                                                                        gCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGcc                                                >  2:1361593/1‑140 (MQ=255)

                                                                                         cTAGCGAAGTGAAAAAGAACGCCATATTTATTGATGATTGATCCGGCGTT‑GGGGG‑GTAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCTCCTGTATTGGTGACGccc                                               >  2:3109254/1‑140 (MQ=255)

                                                                                         cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTCATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGccc                                               >  1:414305/1‑140 (MQ=255)

                                                                                         cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTTTCACCTGTATTGGTGACGccc                                               >  1:2136852/1‑140 (MQ=255)

                                                                                         cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTTTCACCTGTATTGGTGACGccc                                               >  2:2749700/1‑140 (MQ=255)

                                                                                         cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACTTGTATTGGTGACGccc                                               >  1:4627936/1‑140 (MQ=255)

                                                                                         cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGccc                                               >  2:1266023/1‑140 (MQ=255)

                                                                                         cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGccc                                               >  1:3777324/1‑140 (MQ=255)

                                                                                         cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGccc                                               >  2:3921837/1‑140 (MQ=255)

                                                                                         cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGccc                                               >  1:414524/1‑140 (MQ=255)

                                                                                           aGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTg                                             <  1:1998439/140‑1 (MQ=255)

                                                                                           aGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTg                                             >  2:2998752/1‑140 (MQ=255)

                                                                                           aGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGCATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTg                                             >  2:688037/1‑140 (MQ=255)

                                                                                            gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGt                                                                   >  1:2774715/1‑117 (MQ=255)

                                                                                            gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGa                                            >  2:2909271/1‑140 (MQ=255)

                                                                                            gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGa                                            >  1:4812060/1‑140 (MQ=255)

                                                                                            gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGa                                            >  1:3939782/1‑140 (MQ=255)

                                                                                            gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGa                                            >  2:4094104/1‑140 (MQ=255)

                                                                                                 ttAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCa                                       >  1:4639522/1‑140 (MQ=255)

                                                                                                 ttAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCa                                       >  1:3206447/1‑140 (MQ=255)

                                                                                                 ttAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCa                                       >  1:2339776/1‑140 (MQ=255)

                                                                                                 ttAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCa                                       >  1:587578/1‑140 (MQ=255)

                                                                                                           aCGCCATATTTATTGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCAc                             >  2:4170038/1‑140 (MQ=255)

                                                                                                            cGCCATATTTATTGATGATTGACCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCTCCTGATTCGGCGACGACCTGGAACCACGAGTGGAACg                            <  2:3149846/140‑1 (MQ=255)

                                                                                                               cctatttattGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGTGCATGAAGTAATGGAGTATTAGTTCTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACgaga                         <  2:2240383/138‑1 (MQ=255)

                                                                                                                 tatttattGATGATTGATCCCCCGGTGGGGGG‑G‑AGACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGCGACt                       >  2:4380927/1‑140 (MQ=255)

                                                                                                                 tatttattGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACt                       <  1:3999182/140‑1 (MQ=255)

                                                                                                                    ttattGATGATTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCg                    >  2:1174504/1‑140 (MQ=255)

                                                                                                                         gatgatTGATCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGtgctg               >  1:3517851/1‑140 (MQ=255)

                                                                                                                            gattgatCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTa            >  1:3484442/1‑140 (MQ=255)

                                                                                                                             attgatCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTAc           >  2:4804648/1‑140 (MQ=255)

                                                                                                                             attgatCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTAc           >  2:4122806/1‑140 (MQ=255)

                                                                                                                             attgatCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTAc           >  2:2808161/1‑140 (MQ=255)

                                                                                                                             attgatCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTAc           >  1:4456663/1‑140 (MQ=255)

                                                                                                                             attgatCCCCCGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCGGTATTGGTGACGCCCTGAAATCAAGAATGGCACGGGACTCCGTGCTGCTAc           >  1:4017344/1‑140 (MQ=255)

                                                                                                                                     cccGGTGGGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGaaaaa   >  1:2559925/1‑140 (MQ=255)

GCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGT‑GGGGG‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAAC  >  NZ_CP025268/320471‑320743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`