BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,137,115	+T	coding (680/744 nt)	yghT →	dTMP kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,137,115	1	.	T	98.1%	1146.9 / 9.5	309	coding (680/744 nt)	yghT	dTMP kinase
Reads supporting (aligned to +/- strand): ref base . (5/1); new base T (142/161); total (147/162)
Fisher's exact test for biased strand distribution p-value = 1.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 320 total aligned reads displayed.

GAATCGCTGGCGAGGAAGATTGCCATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑G‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCAC  >  NZ_CP025268/3136976‑3137248

gAATCGCTGGCGAGGAAGATTGCCATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACcgcgc‑g                                                                                                                                        <  2:4107600/140‑1 (MQ=255)

gAATCGCTGGCGAGGAAGATTGCCATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACcgcgc‑g                                                                                                                                        <  1:1837007/140‑1 (MQ=255)

gAATCGCTCGCGAGGAAGATTGCCATAACGCCACAGTTAACCTTTGGTGGTGCACACCTGGTTGATATCGATGCCAATCAGCCACTGGAACTGGTGTTGGTTGATGCAGAAACAGCGATTACGGATTTTATGACcgcgc‑g                                                                                                                                        <  2:3742198/140‑1 (MQ=255)

      cTGGCGAGGAAGATTGCCATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTa                                                                                                                                  >  1:1230375/1‑140 (MQ=255)

            aGGAAGATTGCCATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTa                                                                                                                            <  1:4137209/140‑1 (MQ=255)

                         tAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGaa                                                                                                               >  1:2924411/1‑140 (MQ=255)

                           aCGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGAGATCGATGCCAAGCGGCCACTGTGACAGGTGGTGGTGGATGGAGAAAAAGGGGTTACGGGGTTTATGATCTCGCTT‑GGTTATCACGAGTTAAAGATGGAAAt                                                                                                             >  2:3245041/1‑140 (MQ=255)

                               cacaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATGAGCCACTGAAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTGAAAAATGGAAATGccc                                                                                                         >  2:4008567/1‑140 (MQ=255)

                                acaGTTTACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCCATCAGCCACTGGAACAGGTGTTCGTTGATGCAGACAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                        <  1:3906896/140‑1 (MQ=255)

                                acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCGAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCATTAGTCAAAAATGGAAATGCCCg                                                                                                        <  1:3885628/140‑1 (MQ=255)

                                acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCTCGGGAACAGGTGCTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCTC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                        <  2:74399/140‑1 (MQ=255)

                                acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                        <  1:3882545/140‑1 (MQ=255)

                                acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                        <  1:4854002/140‑1 (MQ=255)

                                acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                        <  2:1483704/140‑1 (MQ=255)

                                acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                        <  1:3205459/140‑1 (MQ=255)

                                acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                        <  2:3171928/140‑1 (MQ=255)

                                acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                        <  1:950405/140‑1 (MQ=255)

                                acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                        <  2:3488849/140‑1 (MQ=255)

                                acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                        <  1:1187169/140‑1 (MQ=255)

                                acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                        <  1:1631958/140‑1 (MQ=255)

                                 caGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGa                                                                                                       >  1:1719481/1‑140 (MQ=255)

                                   gTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGGGTTGGTTTATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATc                                                                                                     >  2:2802256/1‑140 (MQ=255)

                                    ttAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATTACCGCTC‑GTGTTTATCACTAGTCAAAAATGGTAATGTCCGATCg                                                                                                    >  1:979371/1‑140 (MQ=255)

                                    ttAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCg                                                                                                    >  1:490783/1‑140 (MQ=255)

                                            tggtggTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                        <  1:1574980/128‑1 (MQ=255)

                                            tggtggTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGAcc                                                                                            >  2:2428170/1‑140 (MQ=255)

                                            tggtggTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGAcc                                                                                            >  2:2142738/1‑140 (MQ=255)

                                            tggtggTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGAcc                                                                                            >  1:1763629/1‑140 (MQ=255)

                                            tggtggTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTAGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGAcc                                                                                            >  1:2967222/1‑140 (MQ=255)

                                            tggtggTGCACAACTGGTTGATAACGATGCCAATCAGCCACTGGAACAGGTGTTGGGTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGG‑GTGGTGATCACTAGTCAAAAATGGAAATGCGGGATCGCCAGGaac                                                                                            >  2:2658440/1‑138 (MQ=255)

                                                   gCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGAGTTGGTTGATGCAGGAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCAtt                                                                                     >  2:3633775/1‑140 (MQ=255)

                                                    cacaACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCAttt                                                                                    >  2:739671/1‑140 (MQ=255)

                                                         cTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAgg                                                                               <  2:2006486/140‑1 (MQ=255)

                                                         cTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAgg                                                                               >  1:3095368/1‑140 (MQ=255)

                                                         cTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGCTGGTTGACGCAGACCAAGCGATTACGGATTTTATGACCGCGCGG‑GGTTAGCACTAGTCAAAAATGGAAATACCCGATCGCCCGGACCGTGCAGTTTCAgg                                                                               >  2:2310012/1‑140 (MQ=255)

                                                          tGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGa                                                                              <  2:3676844/140‑1 (MQ=255)

                                                           ggTTGATATCGATGCCAATCATCCACTGGACCAGGGGTTGGTCGATGCAGAAAAAGCGCTTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGCCAAAAGTGGAGATGCCTGATCGCCAGGACCGGGCAGTTGTAGGaa                                                                             >  2:1292113/1‑140 (MQ=255)

                                                            gttgttATCGATGCCAATCAGCCACTGGTACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGCGG‑GGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGTACCGGGGATTTTCAGGAAg                                                                            >  2:2049659/1‑140 (MQ=255)

                                                            gTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAg                                                                            >  2:2231827/1‑140 (MQ=255)

                                                            gTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAg                                                                            >  2:2733913/1‑140 (MQ=255)

                                                            gTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATGACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAg                                                                            >  2:428581/1‑140 (MQ=255)

                                                               gATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGtt                                                                         <  2:343341/140‑1 (MQ=255)

                                                                 tatCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTaa                                                                       >  1:836793/1‑140 (MQ=255)

                                                                 tatCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTaa                                                                       >  1:4077359/1‑140 (MQ=255)

                                                                    cggtgacAATCAGCCACTGGAACAGTTGTTGGTTGATGCAGAAAAATCTATTACGGTTTTTATGACCGCTC‑GTGGTTATCTCTAGTCGTAAATGGAAGTGCCCGATCACCAGGACCGGGCATTTTCAGGAAGGTTAAATc                                                                    <  2:853174/134‑1 (MQ=255)

                                                                    cGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATc                                                                    <  1:1321269/140‑1 (MQ=255)

                                                                      aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCaa                                                                  >  1:2240675/1‑140 (MQ=255)

                                                                      aTGCCAATCAGCCACTGGAACAGGTGGTGGTTGATGCAGAAAAAGCGATTGCGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCaa                                                                  >  2:4099125/1‑140 (MQ=255)

                                                                         ccAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                               <  1:739671/140‑1 (MQ=255)

                                                                         ccAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                               <  2:1858687/140‑1 (MQ=255)

                                                                         ccAATCAGCCACTGGAACAGGTATTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAATTCCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                               <  2:1477041/140‑1 (MQ=255)

                                                                             tCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCgg                                                                                          <  1:3735937/109‑1 (MQ=255)

                                                                              cAGCCACTGTAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                          <  2:4624698/140‑1 (MQ=255)

                                                                              cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                          <  2:177573/140‑1 (MQ=255)

                                                                              cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                          <  1:3400124/140‑1 (MQ=255)

                                                                              cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                          <  1:2416572/140‑1 (MQ=255)

                                                                              cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                          <  1:1989849/140‑1 (MQ=255)

                                                                              cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                          <  2:2372264/140‑1 (MQ=255)

                                                                              cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                          <  2:2846777/140‑1 (MQ=255)

                                                                              cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                          <  2:2882850/140‑1 (MQ=255)

                                                                              cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                          <  1:3796311/140‑1 (MQ=255)

                                                                              cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                          <  1:4012472/140‑1 (MQ=255)

                                                                              cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                          <  1:1144226/140‑1 (MQ=255)

                                                                              cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                          <  1:1467193/140‑1 (MQ=255)

                                                                              cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                          <  2:4983181/140‑1 (MQ=255)

                                                                              cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                          <  1:819058/140‑1 (MQ=255)

                                                                              cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                          <  2:648779/140‑1 (MQ=255)

                                                                              cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTAACACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                          <  2:4707249/140‑1 (MQ=255)

                                                                              cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTATATGACCGCGC‑GTGGTTAACACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                          <  1:522641/140‑1 (MQ=255)

                                                                                 ccACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTa                                                       <  2:4061641/140‑1 (MQ=255)

                                                                                 ccACTGGAACAGGGGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCAGTTTCAGGAAGGTTAAATCAACTGCAATGCTa                                                       >  1:2603101/1‑140 (MQ=255)

                                                                                       gAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGt                                                 >  1:2497385/1‑140 (MQ=255)

                                                                                        aaCAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTa                                                >  1:4428348/1‑140 (MQ=255)

                                                                                               gttggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGACATTTTCAGGAAGGTTAAATCAACGGCAATGCTATCCAGTACAGccaa                                         >  1:914887/1‑138 (MQ=255)

                                                                                               gttggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGCTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTTCAATGCTATCCAGTACAGCcca                                         >  2:4024178/1‑140 (MQ=255)

                                                                                                  ggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGTACCGGGCCTTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTTCAGCccacca                                      >  2:3316242/1‑140 (MQ=255)

                                                                                                       aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCTCCAGGACCGGGCATTTGCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCagaaag                                 >  2:1603704/1‑140 (MQ=255)

                                                                                                       aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCAGCagaaaa                                 >  2:4815667/1‑139 (MQ=255)

                                                                                                       aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCagaaag                                 >  2:90684/1‑140 (MQ=255)

                                                                                                       aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCagaaag                                 >  1:429951/1‑140 (MQ=255)

                                                                                                        tGCAGAAAAAGCGATTACGGATTTTCTGACCGCGC‑GTGGGTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGATGGTGAAATCAATTGCAATGCTATCCAGTACAGCCCCCCagaagga                                >  2:1002276/1‑140 (MQ=255)

                                                                                                        tGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGGTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTAGCCAGTACAGCCCACCagaaaga                                >  2:3132813/1‑140 (MQ=255)

                                                                                                             aaaaaGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAAt                           >  2:3827858/1‑140 (MQ=255)

                                                                                                                 aGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgcc                       <  1:4984689/140‑1 (MQ=255)

                                                                                                                 aGCGATGACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgcc                       >  1:2330821/1‑140 (MQ=255)

                                                                                                                  gCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACGAGAAAGAAAAATTgccg                      >  2:2943615/1‑140 (MQ=255)

                                                                                                                  gCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgccg                      <  2:1376940/140‑1 (MQ=255)

                                                                                                                  gCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgccg                      <  2:4516843/140‑1 (MQ=255)

                                                                                                                     aTTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGGTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgccgcct                   >  1:1374077/1‑139 (MQ=255)

                                                                                                                       tACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgt                 <  1:757578/140‑1 (MQ=255)

                                                                                                                        aCGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgta                >  2:2538472/1‑140 (MQ=255)

                                                                                                                        aCGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgta                <  2:395413/140‑1 (MQ=255)

                                                                                                                        aCGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgta                <  1:3256069/140‑1 (MQ=255)

                                                                                                                         cGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgtaa               <  1:3678932/140‑1 (MQ=255)

                                                                                                                             ttttATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgtaaagta           <  1:4343784/140‑1 (MQ=255)

                                                                                                                                tATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                          <  2:4340980/90‑1 (MQ=255)

                                                                                                                                tATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAAt        >  1:4673777/1‑140 (MQ=255)

                                                                                                                                     ccGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCa   <  1:2061024/140‑1 (MQ=255)

                                                                                                                                     ccGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCa   <  2:1127771/140‑1 (MQ=255)

                                                                                                                                      cgcgc‑gtGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCAc  >  2:3557517/1‑140 (MQ=255)

GAATCGCTGGCGAGGAAGATTGCCATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑G‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCAC  >  NZ_CP025268/3136976‑3137248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`