Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268448,5060TC21.1% 774.7 / 49.1 327M396V (ATG→GTG) cyoBcytochrome o ubiquinol oxidase subunit I
Reads supporting (aligned to +/- strand):  ref base T (136/122);  new base C (0/69);  total (136/191)
Fisher's exact test for biased strand distribution p-value = 1.42e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 330 total aligned reads displayed.

TTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTGTGGTGATA  >  NZ_CP025268/448367‑448645
                                                                                                                                           |                                                                                                                                           
ttAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCAt                                                                                                                                             <  2:1087461/140‑1 (MQ=255)
   ggCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATccc                                                                                                                                          <  1:698191/140‑1 (MQ=255)
      caccaGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGcc                                                                                                                                       <  1:2473185/140‑1 (MQ=255)
         caGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCAc                                                                                                                                    >  1:2944149/1‑140 (MQ=255)
         caGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCAc                                                                                                                                    >  1:2727137/1‑140 (MQ=255)
              ggTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCgaga                                                                                                                               >  2:2559606/1‑140 (MQ=255)
                   tCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCgag                                                                                                                                >  1:3558195/1‑134 (MQ=255)
                   tCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCgag                                                                                                                                <  2:3558195/134‑1 (MQ=255)
                   tCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTg                                                                                                                          >  1:4349059/1‑140 (MQ=255)
                   tCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTg                                                                                                                          >  1:457829/1‑140 (MQ=255)
                   tCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTg                                                                                                                          >  1:4922414/1‑140 (MQ=255)
                   tCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTg                                                                                                                          >  2:113476/1‑140 (MQ=255)
                   tCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTg                                                                                                                          >  1:1026298/1‑140 (MQ=255)
                   tCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTg                                                                                                                          >  1:861885/1‑140 (MQ=255)
                      cTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACg                                                                                                                       >  1:1955650/1‑140 (MQ=255)
                         cGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGGGACGATa                                                                                                                    >  2:487190/1‑140 (MQ=255)
                                gcCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCTTTTTTCCTGTTACGGCGACCACCGAGAAGGTGACGATAAAACCGa                                                                                                             <  2:2616017/140‑1 (MQ=255)
                                gcCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCTGTTTGCAAGTTACGGCGCCCACCGAGAAGGTGACGATAAAACCGa                                                                                                             <  2:525610/140‑1 (MQ=255)
                                gcCGAAGACCACGCCGCCGATGACCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCCCCGAGAAGGTGACGATAAAACCGa                                                                                                             <  1:4135544/140‑1 (MQ=255)
                                ccccaaGACCACGCCGCAGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCTTTATTAATGATACGGCGCCCACCGAGAAGGTGACGATAAAACCGa                                                                                                             <  2:3759099/136‑1 (MQ=255)
                                 ccGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGAt                                                                                                            >  2:4374451/1‑140 (MQ=255)
                                     aGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTc                                                                                                        <  1:900536/140‑1 (MQ=255)
                                       aCCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCCGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGACAGTCACCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCca                                                                                                      <  2:1819256/140‑1 (MQ=255)
                                           cgccgcCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGc                                                                                                  >  1:623960/1‑140 (MQ=255)
                                           cgccgcCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGc                                                                                                  >  1:3173338/1‑140 (MQ=255)
                                            gccgccGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCTGTATTACAGACACGGCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCcacatca                                                                                                 <  2:2571639/140‑4 (MQ=255)
                                            gccgccGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCa                                                                                                 <  1:4735565/140‑1 (MQ=255)
                                                 cgATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAACCCGATGGTCCACAGCATCGCa                                                                                            <  1:2873498/140‑1 (MQ=255)
                                                 cgATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCAAGTCACCCCGACCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCa                                                                                            <  2:552535/140‑1 (MQ=255)
                                                   atgatCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAACCCGCTGGTCCACAGCATCGCAGa                                                                                          >  1:4055178/1‑140 (MQ=255)
                                                      atCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCTGTTTGAATGATACGGCGACCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATg                                                                                       <  2:396307/140‑1 (MQ=255)
                                                              aTGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCTCCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGa                                                                               >  1:155752/1‑140 (MQ=255)
                                                              aTGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGa                                                                               >  1:5087035/1‑140 (MQ=255)
                                                                tgAAGTGCGCAATCAGGAACAGGCTTTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCTGTTCTCATGTCACCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATg                                                                             <  2:1274893/139‑1 (MQ=255)
                                                                ggAAGTGCGCAATCAGGAACAGGCTGTTCTGCAGAACCCAGTCCGCGCCCGGTACGGCCAGCTTTTTTAATGTTACGGCGACCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATg                                                                             <  2:1358027/140‑1 (MQ=255)
                                                                ggAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATg                                                                             <  1:3915880/140‑1 (MQ=255)
                                                                ggAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATg                                                                             <  2:3819144/140‑1 (MQ=255)
                                                                 gAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCTAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGc                                                                            <  1:1386647/140‑1 (MQ=255)
                                                                 gAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGc                                                                            <  1:2242629/140‑1 (MQ=255)
                                                                 gAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGc                                                                            <  1:4504568/140‑1 (MQ=255)
                                                                 gAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCACCGCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGc                                                                            <  2:938897/140‑1 (MQ=255)
                                                                 gAAGTGCGCAATCACGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCACGCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGc                                                                            <  2:3254395/140‑1 (MQ=255)
                                                                   aGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCgg                                                                          <  1:1790119/140‑1 (MQ=255)
                                                                   aGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCgg                                                                          <  1:3970486/140‑1 (MQ=255)
                                                                   aGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGCCCGCGCCCGGTACGGCCAGCTGTACGAATGATACGGCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCgg                                                                          <  2:863367/140‑1 (MQ=255)
                                                                     tacgcCATAAGGACCCGGCTGTTATGCAGAACGAACTCCGCGCCCGGTACGGCCAGCAGTAAGTCCGTCACCCCGACCACCGAGCAGGTGACGATAAAGCCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGcc                                                                        <  2:4267408/138‑1 (MQ=255)
                                                                     tGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGcc                                                                        <  1:4459079/140‑1 (MQ=255)
                                                                     tGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGcc                                                                        >  1:1242728/1‑140 (MQ=255)
                                                                     tGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGcc                                                                        >  2:2769182/1‑140 (MQ=255)
                                                                     tGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGcc                                                                        >  1:4861485/1‑140 (MQ=255)
                                                                     tGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGcc                                                                        >  1:1600390/1‑140 (MQ=255)
                                                                     tGCGCAATCAGAAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCTGTTCTCAAGTTACGGCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGcc                                                                        <  2:622007/140‑1 (MQ=255)
                                                                     ggcgcAATCAGGGACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCGCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGcc                                                                        <  2:4689060/139‑1 (MQ=255)
                                                                      gcgcAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGccc                                                                       >  1:4206487/1‑140 (MQ=255)
                                                                       cgcAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCTGCACGACAGTTACCGCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCt                                                                      <  2:3491013/140‑1 (MQ=255)
                                                                       cgcAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCt                                                                      >  1:301120/1‑140 (MQ=255)
                                                                          caTCAGGAAACGGCTGTTAGTCAGCACGAAGTTCGCGCCCGGTACGGCCAGCTTTTTGAATGATACGGCGACCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGAt                                                                   <  2:1776224/139‑1 (MQ=255)
                                                                          aaTCAGAAACAGGCTGTTATGCAGAACGAACTCCGCGCCCGGTACTGACAGCTTCTTTAAAGATATGGCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGAt                                                                   <  2:4434041/140‑1 (MQ=255)
                                                                            tCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCa                                                                                            <  2:4202244/113‑1 (MQ=255)
                                                                            tCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATAc                                                                 >  1:87576/1‑140 (MQ=255)
                                                                             cAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACa                                                                <  1:113476/140‑1 (MQ=255)
                                                                              aGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACAt                                                               <  1:2711663/140‑1 (MQ=255)
                                                                                 aaCAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATggg                                                            >  1:2027457/1‑139 (MQ=255)
                                                                                    aGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGaa                                                         >  1:1011250/1‑140 (MQ=255)
                                                                                    aGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGaa                                                         <  1:2769182/140‑1 (MQ=255)
                                                                                      gCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGa                                                                                                             <  1:4375857/86‑1 (MQ=255)
                                                                                      gCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACa                                                       >  1:3468219/1‑140 (MQ=255)
                                                                                      gCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACa                                                       >  1:3387308/1‑140 (MQ=255)
                                                                                        tGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGc                                                     <  2:3226769/140‑1 (MQ=255)
                                                                                        tGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGc                                                     <  2:4794472/140‑1 (MQ=255)
                                                                                         gTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCTTTTTTAATGATACGGCGACCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGcc                                                    <  2:2138/140‑1 (MQ=255)
                                                                                              gCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTg                                               <  1:1494234/140‑1 (MQ=255)
                                                                                                 gAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAg                                            <  1:3321723/140‑1 (MQ=255)
                                                                                                 gAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAg                                            <  1:2654562/140‑1 (MQ=255)
                                                                                                  aaCGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGa                                           >  2:5061/1‑140 (MQ=255)
                                                                                                  aaCGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGAGGGTCGACAGCATCGAAGAATGGAACACGATGCGGCCAGGAGACAAGGTGAACGGCCGGTCGAAgc                                           >  2:1978525/1‑139 (MQ=255)
                                                                                                    cGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATc                                         >  1:1855740/1‑140 (MQ=255)
                                                                                                    cGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATc                                         >  1:1156292/1‑140 (MQ=255)
                                                                                                    cGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGGTGAAGATc                                         >  1:547793/1‑140 (MQ=255)
                                                                                                    cGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCACCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATc                                         <  2:2312221/140‑1 (MQ=255)
                                                                                                            gcgcCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGt                                                                                                                           <  1:4777803/50‑1 (MQ=255)
                                                                                                            gcgcCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGt                                                                                                                           >  2:4777571/1‑50 (MQ=255)
                                                                                                               cccGGTACGGCCAGCAGCACGCCAGTCACCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGt                              <  2:2004004/140‑1 (MQ=255)
                                                                                                                   gTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAAGGGAACGCGATGAGGCTTGGATTGATGGTGAACAGCGAGTTGACGATCGTCCCCCCggccagg                          >  2:2708242/1‑135 (MQ=255)
                                                                                                                    tACGGCCAGCAGCTCGACTGTCACCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGACCAGCCAGTTGAAGATCTTCACCCCGGTCGGGa                         <  2:22695/140‑1 (MQ=255)
                                                                                                                    tACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGa                         <  1:214832/140‑1 (MQ=255)
                                                                                                                    tACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGa                         <  1:3224353/140‑1 (MQ=255)
                                                                                                                           agcagcACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGAt                  <  2:1497769/140‑1 (MQ=255)
                                                                                                                            gcagcaCGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATa                 >  1:343666/1‑140 (MQ=255)
                                                                                                                            gcagcaCGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATa                 <  1:73397/140‑1 (MQ=255)
                                                                                                                             cagcaCGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCACTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATaa                >  1:3647247/1‑140 (MQ=255)
                                                                                                                              aggaCGCCGGTCATCCCACCTCCGGAGAAGGTCACGATGAAGCCGATGGTCCACAGCATCGCTGAGGGGAACACGATCCGGCCCTGGTACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTAGGGATGGCGATAAt               <  2:1265640/137‑1 (MQ=255)
                                                                                                                              agcaCGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAAt               >  1:1441832/1‑140 (MQ=255)
                                                                                                                                caCGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCa             <  2:4096426/140‑1 (MQ=255)
                                                                                                                                  cGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCAtt           <  2:3355551/140‑1 (MQ=255)
                                                                                                                                  cGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCAtt           >  1:32929/1‑140 (MQ=255)
                                                                                                                                      aGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTgtgg       <  1:4000994/140‑1 (MQ=255)
                                                                                                                                          aTCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTGTGGTGAt   <  1:1713271/140‑1 (MQ=255)
                                                                                                                                          aTCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTGTGGTGAt   >  1:4541651/1‑140 (MQ=255)
                                                                                                                                           tCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTGTGGTGATa  <  2:1696903/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTGTGGTGATA  >  NZ_CP025268/448367‑448645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑