Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 745,775 A→G *264* (TAA→TAG) 
A348A (GCT→GCC) 		 nei →
abrB ←		 endonuclease VIII
AbrB family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268745,7750AG99.7% 998.7 / NA 303*264* (TAA→TAG) 
A348A (GCT→GCC) 		nei
abrB		endonuclease VIII
AbrB family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (129/173);  minor base C (1/0);  total (130/173)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 303 total aligned reads displayed.

ATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTCCCTGCGGCGATGAC  >  NZ_CP025268/745639‑745906
                                                                                                                                        |                                                                                                                                   
   aGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGAc                                                                                                                               <  1:2697215/140‑1 (MQ=255)
   aGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGAc                                                                                                                               <  2:2801148/140‑1 (MQ=255)
     catcatGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCg                                                                                                                             <  1:1998029/140‑1 (MQ=255)
       tcatGGGGCGCTGTTTCGCTTTAAGGTGTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCt                                                                                                                           <  2:3141776/140‑1 (MQ=255)
        tatGGGGCGCTGTTTAGCTTTAAGGTTCTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACAGCtt                                                                                                                          <  2:3677938/139‑1 (MQ=255)
        catGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCtt                                                                                                                          <  1:1361044/140‑1 (MQ=255)
        catGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCtt                                                                                                                          <  1:2472415/140‑1 (MQ=255)
         atGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATGATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTc                                                                                                                         >  2:4542617/1‑140 (MQ=255)
         atGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTc                                                                                                                         >  1:4884660/1‑140 (MQ=255)
         atGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTc                                                                                                                         >  1:869802/1‑140 (MQ=255)
         atGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTc                                                                                                                         >  1:435714/1‑140 (MQ=255)
         atGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTc                                                                                                                         >  1:3743424/1‑140 (MQ=255)
         atGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTc                                                                                                                         >  2:5094568/1‑140 (MQ=255)
         atGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTc                                                                                                                         >  2:2002628/1‑140 (MQ=255)
         atGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTc                                                                                                                         >  2:2658500/1‑140 (MQ=255)
         atGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTc                                                                                                                         >  2:2959725/1‑140 (MQ=255)
         atGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTc                                                                                                                         >  1:1236705/1‑140 (MQ=255)
             ggCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGgcg                                                                                                                     >  2:3230045/1‑140 (MQ=255)
               cgcTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCa                                                                                                                   <  1:933611/140‑1 (MQ=255)
               cgcTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCa                                                                                                                   <  2:1473782/140‑1 (MQ=255)
               cgcTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCa                                                                                                                   <  1:1764243/140‑1 (MQ=255)
               cgcTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCa                                                                                                                   <  1:3752707/140‑1 (MQ=255)
                 cTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATa                                                                                                                 >  1:3657473/1‑140 (MQ=255)
                   gTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAgg                                                                                                               <  1:2959725/140‑1 (MQ=255)
                   gTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAgg                                                                                                               <  1:3888236/140‑1 (MQ=255)
                   gTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAgg                                                                                                               <  2:2627411/140‑1 (MQ=255)
                   gTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAgg                                                                                                               <  2:5046778/140‑1 (MQ=255)
                   gTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAgg                                                                                                               <  1:4641049/140‑1 (MQ=255)
                   gTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAgg                                                                                                               <  1:2566738/140‑1 (MQ=255)
                   gTTTCGCTGTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAgg                                                                                                               <  2:1269690/140‑1 (MQ=255)
                        gCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGaa                                                                                                          <  1:501781/140‑1 (MQ=255)
                         cTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGaaa                                                                                                         >  2:4734336/1‑140 (MQ=255)
                            tAAGGGGTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGCGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGTCCGACCTCTTCGGCGCAAAGGTTGAATTaa                                                                                                      >  2:475390/1‑140 (MQ=255)
                             aaGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATaaa                                                                                                     <  1:4016299/140‑1 (MQ=255)
                              aGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAAc                                                                                                    <  1:4839219/140‑1 (MQ=255)
                              aGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAAc                                                                                                    >  2:1482566/1‑140 (MQ=255)
                              aGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAAc                                                                                                    <  1:1139219/140‑1 (MQ=255)
                              aGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAAc                                                                                                    <  1:2338850/140‑1 (MQ=255)
                                  ttttCATCGTGAAGGCTAATCTTGCGTTCGTTGTGGCATCATCATTTAGGACACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAACAAACcgcg                                                                                                <  2:3616612/140‑1 (MQ=255)
                                  ttttCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACcgcg                                                                                                <  1:2591743/140‑1 (MQ=255)
                                  ttttCAGCGTAACGGCGAGCCGGGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTCTCTTGCCGCCCGTTTGCCTGGTGCCCGGGCTGCCAGCTCGAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACcgcg                                                                                                <  2:3449018/140‑1 (MQ=255)
                                   tttCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCgcgc                                                                                               >  2:2706348/1‑140 (MQ=255)
                                    ttCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAACACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCa                                                                                              <  2:2863644/140‑1 (MQ=255)
                                    ttCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCa                                                                                              <  1:2140350/140‑1 (MQ=255)
                                        tCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATgg                                                                                          <  1:2789155/140‑1 (MQ=255)
                                         cGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGc                                                                                         >  1:478639/1‑140 (MQ=255)
                                         cGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGc                                                                                         >  1:475352/1‑140 (MQ=255)
                                            gaTGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAgg                                                                                      <  2:1767100/140‑1 (MQ=255)
                                                  gAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGt                                                                                <  1:4930051/140‑1 (MQ=255)
                                                  gAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGt                                                                                <  1:21225/140‑1 (MQ=255)
                                                  gAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGt                                                                                <  1:20817/140‑1 (MQ=255)
                                                  gAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGt                                                                                <  2:3353778/140‑1 (MQ=255)
                                                  gAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGt                                                                                <  1:661702/140‑1 (MQ=255)
                                                      cGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCGGGCATCGCGCCCGTTGTACTGGTGCCCTGGCTGCCAGCACGAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAAGGGCAGGCCCTGGCAGc                                                                            >  2:217575/1‑140 (MQ=255)
                                                       gTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCa                                                                           <  2:3992713/140‑1 (MQ=255)
                                                       gTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCa                                                                           <  1:2637653/140‑1 (MQ=255)
                                                       gTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCa                                                                           <  1:2691752/140‑1 (MQ=255)
                                                            aaCGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATa                                                                      <  2:4860068/140‑1 (MQ=255)
                                                                 tgtgGCAGCATCATTGAGAAAACCCCGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGaa                                                                 <  2:887061/140‑1 (MQ=255)
                                                                 tgtgGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTCCCGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGaa                                                                 <  2:2066642/140‑1 (MQ=255)
                                                                 tgtgGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGaa                                                                 <  1:1603019/140‑1 (MQ=255)
                                                                 tgtgGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGaa                                                                 <  1:1652850/140‑1 (MQ=255)
                                                                 tgtgGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGaa                                                                 <  2:768363/140‑1 (MQ=255)
                                                                 tgtgGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGaa                                                                 <  1:1014931/140‑1 (MQ=255)
                                                                 tgtgGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGaa                                                                 <  1:2938457/140‑1 (MQ=255)
                                                                 tgtgGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGaa                                                                 <  2:2361313/140‑1 (MQ=255)
                                                                 tgtgGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGaa                                                                 <  1:3934383/140‑1 (MQ=255)
                                                                 tgtgGAAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCTCTTCGGCTCATAGGTTGAAATAAACCGCGCAATGGCAGTTCTTGTCAGTAAAATACTGaa                                                                 <  2:1957678/140‑1 (MQ=255)
                                                                   tgGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACa                                                               <  1:1491177/140‑1 (MQ=255)
                                                                        gcaTCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGt                                                          <  2:4671852/140‑1 (MQ=255)
                                                                        gcaTCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGt                                                          <  2:3927359/140‑1 (MQ=255)
                                                                        gcaTCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGt                                                          <  1:2392168/140‑1 (MQ=255)
                                                                             atTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCGGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGGAATGGCAGGCCCTGTCGGCAATATCCTGAACAGGCGTAgggg                                                     >  2:4633422/1‑139 (MQ=255)
                                                                              ttGAGAAAACCCCGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATACGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGtt                                                    >  1:4232936/1‑140 (MQ=255)
                                                                              ttGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGtt                                                    >  1:4127428/1‑140 (MQ=255)
                                                                                  gaAAACCACGCTGTCATCTCGCCCGTTTTACTTGTGCCCTCGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTCAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGcc                                                <  2:4245608/140‑2 (MQ=255)
                                                                                  gaAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCa                                                <  1:3497385/140‑1 (MQ=255)
                                                                                  gaAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCa                                                <  2:4113363/140‑1 (MQ=255)
                                                                                     aaCCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCCGCAAAATACTGAACAGGCGTAGGGTTTGCATCg                                             >  1:1720162/1‑140 (MQ=255)
                                                                                      aCCACGCTGTCATCTCGCCCGTTTTACAGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGc                                            >  2:3758162/1‑140 (MQ=255)
                                                                                        cACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCa                                          >  1:1405846/1‑140 (MQ=255)
                                                                                            cTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAAt                                      <  2:1058343/140‑1 (MQ=255)
                                                                                             tGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATg                                     <  2:2481261/140‑1 (MQ=255)
                                                                                               tCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAg                                   <  2:4800189/140‑1 (MQ=255)
                                                                                                  tctcGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCGGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTATGGTTTGCATCGCCATAATGAgcgc                                <  2:581059/140‑1 (MQ=255)
                                                                                                       cccGTTTTACTTGTGCCCGAGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCatat                           <  2:916578/140‑1 (MQ=255)
                                                                                                       cccGTTTTACTGGTGCCCTGGCTGCCAGCCCTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCatat                           <  1:3106351/140‑1 (MQ=255)
                                                                                                       cccGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATCGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCatat                           <  1:1949061/140‑1 (MQ=255)
                                                                                                       cccGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATCATGAGCGCCatat                           >  2:4434076/1‑140 (MQ=255)
                                                                                                            tttACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCa                      <  1:515841/140‑1 (MQ=255)
                                                                                                              tACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCAtt                    <  2:4332669/140‑1 (MQ=255)
                                                                                                                  ggTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTc                >  1:1048401/1‑140 (MQ=255)
                                                                                                                   gTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTcc               >  2:2552217/1‑140 (MQ=255)
                                                                                                                    tGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTccc              >  1:4521245/1‑140 (MQ=255)
                                                                                                                    tGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTccc              >  1:1525390/1‑140 (MQ=255)
                                                                                                                           gCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTCCCTgcggcg       >  1:333363/1‑140 (MQ=255)
                                                                                                                           gCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAAGGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCGTATCGGCATTGCTCCCTgcggcg       >  2:4367780/1‑140 (MQ=255)
                                                                                                                             tGCCCGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTTTCAGCAAAATTCTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGACATTGCTCCCTgctgcggt     >  2:5007469/1‑138 (MQ=255)
                                                                                                                               ccAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTCCCTGCGGCGATGa   >  2:3502197/1‑140 (MQ=255)
                                                                                                                                cAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTCCCTGCGGCGATGAc  <  2:394434/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                   
ATAAGCATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTCCCTGCGGCGATGAC  >  NZ_CP025268/745639‑745906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑