Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	820,054	A→G	*226* (TAA→TAG)	bioD →	dethiobiotin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	820,054	0	A	G	100.0%	309.8 / NA	92	*226* (TAA→TAG)	bioD	dethiobiotin synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/60);  total (32/60)

CCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGG  >  NZ_CP025268/819917‑820190                                                                                                                                          |                                                                                                                                         ccGGTAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGcc                                                                                                                                        >  2:4840303/1‑140 (MQ=255)  cGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCa                                                                                                                                       <  1:221631/140‑1 (MQ=255)    ggAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCAtt                                                                                                                                     <  1:3366327/140‑1 (MQ=255)    ggAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCAtt                                                                                                                                     <  2:1868148/140‑1 (MQ=255)    ggAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCAtt                                                                                                                                     <  1:3366483/140‑1 (MQ=255)    ggAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCAtt                                                                                                                                     <  2:3271342/140‑1 (MQ=255)    ggAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCAtt                                                                                                                                     <  1:520117/140‑1 (MQ=255)    ggAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCAtt                                                                                                                                     <  1:1760245/140‑1 (MQ=255)    ggAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCAtt                                                                                                                                     <  1:1761324/140‑1 (MQ=255)    ggAAAACATCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCAtt                                                                                                                                     <  1:5023800/140‑1 (MQ=255)     gAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTc                                                                                                                                    <  1:2333115/140‑1 (MQ=255)         aCGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTa                                                                                                                                <  2:2906377/140‑1 (MQ=255)         aCGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTa                                                                                                                                <  2:2906342/140‑1 (MQ=255)         aCGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCAAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTa                                                                                                                                <  1:1193790/140‑1 (MQ=255)           gTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTAtt                                                                                                                              <  2:3845691/140‑1 (MQ=255)           gTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTAtt                                                                                                                              <  1:3874439/140‑1 (MQ=255)               cGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGt                                                                                                                          <  1:1959760/140‑1 (MQ=255)                     atatatGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAAtt                                                                                                                    >  1:2914087/1‑140 (MQ=255)                         atGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTtcga                                                                                                                >  2:4357024/1‑140 (MQ=255)                         atGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTgcga                                                                                                                <  2:2048343/140‑1 (MQ=255)                         atGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTgcga                                                                                                                <  2:2047951/140‑1 (MQ=255)                         atGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTgcga                                                                                                                <  2:4107413/140‑1 (MQ=255)                            aCCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTgcgagcg                                                                                                             >  2:2434083/1‑140 (MQ=255)                              cACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGcgagcgag                                                                                                           >  1:3715572/1‑140 (MQ=255)                              cACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTCCCTTGTTGTAGCCATTCTGTATTTGGATAAATTGcgagcgag                                                                                                           >  2:3893913/1‑140 (MQ=255)                                cGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGAt                                                                                                         >  1:3017244/1‑140 (MQ=255)                                cGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGAt                                                                                                         <  1:4480785/140‑1 (MQ=255)                                cGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGAt                                                                                                         >  2:3202150/1‑140 (MQ=255)                                 gcgcACCCGCATGATTCCCGCGCCGCTGCTAGGAGAGATCCACTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTCTATTTGGTTAAATTGCGAGCGAGATc                                                                                                        <  2:1612492/137‑1 (MQ=255)                                 gCTCACCCGCCTGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGCAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATc                                                                                                        <  1:2560353/140‑1 (MQ=255)                                 gCTCACCCGCATGATTCTCGCGCCGCTGCTGGGACAGATCCCATGGCTTGCAGAAAATCCAGAAAATGCGGAAACCGGAAAGTACATAAACCTTGCCTGGTTGTAGCCATTCTGTATGTGGTTAAATTGCGAGCGAGATc                                                                                                        <  2:4752552/140‑1 (MQ=255)                                 gCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATc                                                                                                        <  2:4889992/140‑1 (MQ=255)                                 gCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATc                                                                                                        <  1:2564478/140‑1 (MQ=255)                                 gCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATc                                                                                                        <  2:4752593/140‑1 (MQ=255)                                 gCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATc                                                                                                        <  2:4293473/140‑1 (MQ=255)                                 gCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATc                                                                                                        <  1:2564691/140‑1 (MQ=255)                                 gCTCACCCCCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAACATCCCGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCATTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATc                                                                                                        <  2:1103571/140‑1 (MQ=255)                                  cTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATcg                                                                                                       >  2:4783026/1‑140 (MQ=255)                                   tCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATcgc                                                                                                      <  1:1490835/140‑1 (MQ=255)                                   tCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATcgc                                                                                                      <  1:1490868/140‑1 (MQ=255)                                     aCCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGt                                                                                                    <  2:60406/140‑1 (MQ=255)                                     aCCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGt                                                                                                    <  1:17277/140‑1 (MQ=255)                                       ccGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTAttt                                                                                                                             <  1:3896965/113‑1 (MQ=255)                                       ccGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTAttt                                                                                                                             >  2:3896965/1‑113 (MQ=255)                                          cATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCg                                                                                               >  2:3798789/1‑140 (MQ=255)                                             gATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGAtt                                                                                            <  1:4654395/140‑1 (MQ=255)                                             gATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGAtt                                                                                            <  1:173285/140‑1 (MQ=255)                                               ttCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGa                                                                                          >  1:5005128/1‑140 (MQ=255)                                                tCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCACCCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGAc                                                                                         <  1:4226919/140‑1 (MQ=255)                                                tCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGAc                                                                                         <  1:949825/140‑1 (MQ=255)                                                 cccGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACt                                                                                        <  2:132122/140‑1 (MQ=255)                                                 cccGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACt                                                                                        <  1:4096457/140‑1 (MQ=255)                                                 cccGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACt                                                                                        <  1:2276628/140‑1 (MQ=255)                                                          ctgctgGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTaa                                                                               >  2:451147/1‑140 (MQ=255)                                                          ctgctgGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTaa                                                                               >  2:2302435/1‑140 (MQ=255)                                                           tgctgGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAAc                                                                              <  1:767232/140‑1 (MQ=255)                                                           tgctgGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAAc                                                                              <  1:3489215/140‑1 (MQ=255)                                                                 gagagaTCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTACATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTa                                                                        <  2:864788/140‑1 (MQ=255)                                                                   gagcTCCCCTGGCTTGCAGAAAACCACGACAATGCGGCAACCGGAAAGTACATAACCCTTGCCCTGTTGTAGCCATTCTGTATTTGGTTCAATTGCGAGCGAGATCGCGTCTTCGATTGACTGAAATTTAACCAATTaaa                                                                      <  2:3024512/140‑1 (MQ=255)                                                                    agaTCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAAt                                                                     >  2:249908/1‑140 (MQ=255)                                                                       tCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCt                                                                  >  1:3523071/1‑140 (MQ=255)                                                                       tCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCt                                                                  >  1:3584708/1‑140 (MQ=255)                                                                       tCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCt                                                                  >  1:3584858/1‑140 (MQ=255)                                                                                 tGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAAtt                                                                    <  2:4136118/128‑1 (MQ=255)                                                                                 tGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAAtt                                                                    >  1:4136118/1‑128 (MQ=255)                                                                                 tGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAAtt                                                                    >  1:4136343/1‑128 (MQ=255)                                                                                 tGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAAtt                                                                    <  2:4136343/128‑1 (MQ=255)                                                                                 tGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCa                                                        >  2:3495051/1‑140 (MQ=255)                                                                                   cAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACg                                                      >  1:3929235/1‑140 (MQ=255)                                                                                        aaTCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGaaaaa                                                 <  1:4632966/140‑1 (MQ=255)                                                                                             aGAAAATGCGGCAACCGGAAAGTACCTCAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTACATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTACCCAATTAAATTCTAAAATAATCACGACAAAAAttt                                            >  1:1882828/1‑140 (MQ=255)                                                                                             aGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAAttt                                            >  1:3145533/1‑140 (MQ=255)                                                                                             aGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAAttt                                            >  1:3145398/1‑140 (MQ=255)                                                                                             aGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAAttt                                            >  2:4458026/1‑140 (MQ=255)                                                                                                    gCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTcc                                     >  2:445600/1‑140 (MQ=255)                                                                                                          aCCGGCAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCa                               <  2:1872725/140‑1 (MQ=255)                                                                                                          aCCGGAAAGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCa                               <  2:1872654/140‑1 (MQ=255)                                                                                                                 aGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATgcggcg                        <  1:4379977/140‑1 (MQ=255)                                                                                                                 aGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATgcggcg                        <  1:2619525/140‑1 (MQ=255)                                                                                                                 aGTACATAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATgcggcg                        <  1:1181934/140‑1 (MQ=255)                                                                                                                      aTAAACCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAAtgt                   <  2:2991467/140‑1 (MQ=255)                                                                                                                          aCCTTGCCTTGTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGa               <  1:709455/140‑1 (MQ=255)                                                                                                                            cttgccttgTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAAt             <  1:2024459/140‑1 (MQ=255)                                                                                                                             ttgccttgTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAACATTTTACTTCCGCCTCCTGCGGCGAATGTGGGAAtt            >  1:2549710/1‑140 (MQ=255)                                                                                                                             ttgccttgTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAAtt            >  1:216667/1‑140 (MQ=255)                                                                                                                             ttgccttgTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAAtt            >  1:1129960/1‑140 (MQ=255)                                                                                                                               gccttgTTGTAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGc          >  2:1866692/1‑140 (MQ=255)                                                                                                                                    gttgtAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAgg     <  2:445439/140‑1 (MQ=255)                                                                                                                                    gttgtAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAgg     >  2:4671605/1‑140 (MQ=255)                                                                                                                                    gttgtAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACAAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAgg     <  2:177270/140‑1 (MQ=255)                                                                                                                                    gttgtAGCCATTCTGTATTTGGTTAAATTCCGAGCAAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGCAAAAAATTTTACTTCCGCCTCATGCGGCGCATGTGGGAATTGCCCAgg     <  2:1796305/140‑1 (MQ=255)                                                                                                                                       gtAGCCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAggcgg  <  1:1947954/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         CCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAGAAAATCCAGAAAATGCGGCAACCGGAAAGTACATAAACCTTGCCTTGTTGTAACCATTCTGTATTTGGTTAAATTGCGAGCGAGATCGCGTCTTCGATTGACTGCAATTTAACCAATTAAATTCTAAAATAATCACGAAAAAAATTTTACTTCCGCCTCATGCGGCGAATGTGGGAATTGCCCAGGCGG  >  NZ_CP025268/819917‑820190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑