BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	974,224	G→A	Q166Q (CAG→CAA)	msbA →	lipid A ABC transporter ATP‑binding protein/permease MsbA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	974,224	0	G	A	100.0%	1350.7 / NA	366	Q166Q (CAG→CAA)	msbA	lipid A ABC transporter ATP‑binding protein/permease MsbA
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (207/159); total (207/159)

Only 100 of 366 total aligned reads displayed.

GTACGCTGTTGTCACGTATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACAAATGCTG  >  NZ_CP025268/974091‑974359

gTACGCTGTTGTCACGTATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCg                                                                                                                                   <  1:3802240/140‑1 (MQ=255)

   cGCTATTGCCACGTATTACCAACGATTCCGCACAGGTTGCTGCGTCTGCTTTCGGTGCACTGATTTCTGTTGTGCGTGAAGGTGGGTCGAGCATCGGCCTGGTCCATACGATTGTCTCTTACCGATGCCTACTGTCGATc                                                                                                                                >  2:3608471/1‑140 (MQ=255)

          gtCACGTATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTCGCAACTGTCGATCATTTTGa                                                                                                                         >  2:3291893/1‑140 (MQ=255)

           tCACGTATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGAt                                                                                                                        <  1:3502147/140‑1 (MQ=255)

           tCACGTATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGAt                                                                                                                        <  2:2179032/140‑1 (MQ=255)

           tCACGTATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGAt                                                                                                                        <  2:3036198/140‑1 (MQ=255)

           tCACGTATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGGCGATCATTTTGAt                                                                                                                        >  2:203570/1‑140 (MQ=255)

             aCGTATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATtg                                                                                                                      >  1:1276047/1‑140 (MQ=255)

             aCGTATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTGGATtg                                                                                                                      >  2:4492257/1‑140 (MQ=255)

              cGTATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATtgt                                                                                                                     >  2:4409043/1‑140 (MQ=255)

               gTATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATtgtg                                                                                                                    <  1:432970/140‑1 (MQ=255)

                tATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGc                                                                                                                   >  2:194786/1‑140 (MQ=255)

                 aTTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCt                                                                                                                  <  1:2024897/140‑1 (MQ=255)

                  ttACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTg                                                                                                                 >  1:145987/1‑140 (MQ=255)

                  ttACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATGTTGATTGTGCTg                                                                                                                 >  1:2330934/1‑140 (MQ=255)

                    aCCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGc                                                                                                               <  1:4976479/140‑1 (MQ=255)

                     ccTACGCTTCCGAACAGGCTGGTTCTTGCTCTTCCGGCGCACTGCTTACAGATGTGCGTGAAGGTGCGTCGATCATGGGCGTGTGCATCATGATGTTCTCTTACAGTTGGCAACTGTCGGTCATTTTGATTGTGCTGGCa                                                                                                              >  2:4752869/1‑140 (MQ=255)

                         cGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGa                                                                                                          >  2:3422960/1‑140 (MQ=255)

                          gATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGAt                                                                                                         <  1:2546006/140‑1 (MQ=255)

                                 aaCAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGGTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCg                                                                                                  >  2:1064122/1‑140 (MQ=255)

                                      gTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGc                                                                                             >  1:2910039/1‑140 (MQ=255)

                                        tGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGa                                                                                           >  1:3594010/1‑140 (MQ=255)

                                            tcttcttcttcCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTcg                                                                                       <  2:3202867/140‑1 (MQ=255)

                                              ttcttcttcCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGCTTGTGCTGGCACCGATTGTTTCGATTGCGATTcgcg                                                                                     <  2:5007350/140‑1 (MQ=255)

                                              ttcttcttcCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTcgcg                                                                                     <  2:1645549/140‑1 (MQ=255)

                                              ttcttcttcCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTcgcg                                                                                     <  2:2540627/140‑1 (MQ=255)

                                              ttcttcttcCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTcgcg                                                                                     <  1:594912/140‑1 (MQ=255)

                                                cttcttcCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGtt                                                                                   >  2:2401770/1‑140 (MQ=255)

                                                cttcttcCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGtt                                                                                   <  2:2620968/140‑1 (MQ=255)

                                                     tcCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATc                                                                              >  2:3357067/1‑140 (MQ=255)

                                                     tcCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATGCGCGTTGTATc                                                                              >  1:3218215/1‑140 (MQ=255)

                                                       cGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGa                                                                            >  1:2683886/1‑140 (MQ=255)

                                                       cGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGa                                                                            >  1:4633639/1‑140 (MQ=255)

                                                         gcgcACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAg                                                                          >  1:774489/1‑140 (MQ=255)

                                                                 aTTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGGTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCg                                                                  >  1:4786883/1‑140 (MQ=255)

                                                                  ttACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGc                                                                 <  1:2196337/140‑1 (MQ=255)

                                                                    aCTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCaa                                                               <  2:1702112/140‑1 (MQ=255)

                                                                     cTGTTGTGCGTTAAGGTGCGTCGATCATCGGACTGTTCATCATGATGTTCTATTACAGTTGGCTACTGTCGATCATTTTGATTGTGCTGGCACAGATTGTTTCGATTGCGATTCGCTTTGTATCGAAGCGTTTTCGCAAc                                                              <  1:5115953/140‑1 (MQ=255)

                                                                     cTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCCATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAAc                                                              <  1:4365050/140‑1 (MQ=255)

                                                                         tgtgCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCa                                                          >  1:4129382/1‑140 (MQ=255)

                                                                          gtgCGTGAAGGTGCGTTGATCATCGGCCCGTTGATCATGACGTTCTATTACAGTTGGCAACTGTCGATCATTTCGATTGCGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCat                                                         <  2:4245264/140‑2 (MQ=255)

                                                                           tgCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCCTGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGAGTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGt                                                        >  1:1191539/1‑140 (MQ=255)

                                                                           tgCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGt                                                        >  1:4657688/1‑140 (MQ=255)

                                                                           tgCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGt                                                        >  1:1619957/1‑140 (MQ=255)

                                                                           tgCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGt                                                        >  1:4110024/1‑140 (MQ=255)

                                                                           tgCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGt                                                        >  1:2581075/1‑140 (MQ=255)

                                                                           tgCGTGAAGGTGCGTCGATCAGCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGt                                                        >  2:2276400/1‑140 (MQ=255)

                                                                            gCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTa                                                       <  1:1015175/140‑1 (MQ=255)

                                                                             cGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTaa                                                      >  1:4505529/1‑140 (MQ=255)

                                                                                gAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTaaaaa                                                   >  2:1980227/1‑140 (MQ=255)

                                                                                 aaGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAAc                                                  <  2:379548/140‑1 (MQ=255)

                                                                                    gTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTCCAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATg                                               <  1:3426575/140‑1 (MQ=255)

                                                                                    gTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATg                                               <  1:1528046/140‑1 (MQ=255)

                                                                                    gTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATg                                               <  1:1503134/140‑1 (MQ=255)

                                                                                    gTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATg                                               <  1:1345319/140‑1 (MQ=255)

                                                                                     tGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTAGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGc                                              <  1:1696455/140‑1 (MQ=255)

                                                                                      gCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCa                                             <  2:4720783/140‑1 (MQ=255)

                                                                                         tctatcatcGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGaa                                          <  2:4943932/137‑1 (MQ=255)

                                                                                         tCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGaa                                          <  2:1364376/140‑1 (MQ=255)

                                                                                         tCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGaa                                          <  1:3356748/140‑1 (MQ=255)

                                                                                         tCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGaa                                          <  1:1042056/140‑1 (MQ=255)

                                                                                            atcatcGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAacac                                       >  2:723794/1‑140 (MQ=255)

                                                                                              catcGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCa                                     <  2:3481484/140‑1 (MQ=255)

                                                                                              catcGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAAATGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCa                                     <  1:3021804/140‑1 (MQ=255)

                                                                                                   gCCTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATgggg                                <  1:4755683/140‑1 (MQ=255)

                                                                                                    ccTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGc                               <  2:777922/140‑1 (MQ=255)

                                                                                                    ccTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGc                               <  2:1529362/140‑1 (MQ=255)

                                                                                                    ccTGTTCATCATGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGCATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGc                               <  2:3956270/140‑1 (MQ=255)

                                                                                                        ttCATCATGATGTTCTATTACAGTTGGCAACTGTCGATTATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGt                           >  1:3928496/1‑140 (MQ=255)

                                                                                                        ttCATCATGATGTTATATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGCTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTAGCAACATCAGTAAAAACATGCAGAACACAATGGGGTAGGt                           >  2:4072454/1‑140 (MQ=255)

                                                                                                         tcatcaTGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTg                          <  2:4828695/140‑1 (MQ=255)

                                                                                                           atcatGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGac                        <  1:1955920/140‑1 (MQ=255)

                                                                                                             catGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGc                               >  1:3246083/1‑131 (MQ=255)

                                                                                                             catGATGTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGc                               <  2:3246252/131‑1 (MQ=255)

                                                                                                              atgatgTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCTCTTCTTTTTTCTATTTCGTTTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAATCATGCCGAACACTATGTGGCAGGTGacccc                     <  2:1581999/140‑3 (MQ=255)

                                                                                                              atgatgTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGaccac                     <  2:1482496/140‑1 (MQ=255)

                                                                                                                  tgTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGTGCAGGTGACCACCAgc                 >  2:3929169/1‑140 (MQ=255)

                                                                                                                  tgTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAgc                 >  1:902837/1‑140 (MQ=255)

                                                                                                                  tgTTCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAgc                 >  2:2912572/1‑140 (MQ=255)

                                                                                                                    ttCTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAgcgc               >  2:4343498/1‑140 (MQ=255)

                                                                                                                    ttCTATTAAAGTTGGCAAGTGACGATCATTTTGATTATGCTGTCACCGATTGTTTCCATTGCGATTCGCGTTGTATCGAAGCATTTTCGCAACATCAGTCAAGTCATACAGAACACCATGGAGCAAGTGACCACCAgcgc               <  2:551646/140‑1 (MQ=255)

                                                                                                                      cTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAg             <  2:4560903/140‑1 (MQ=255)

                                                                                                                      cTATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACAGGCAGAACACCATGGGGCAGGTGACCACCAGCGCAg             >  1:2450092/1‑140 (MQ=255)

                                                                                                                       tATTACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGa            >  2:2698361/1‑140 (MQ=255)

                                                                                                                         ttACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAGAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAAc          >  2:4180734/1‑140 (MQ=255)

                                                                                                                         ttACAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAAc          <  1:2061029/140‑1 (MQ=255)

                                                                                                                            cAGTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACaaa       >  2:1778359/1‑140 (MQ=255)

                                                                                                                              gTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACAAATg     >  1:657830/1‑140 (MQ=255)

                                                                                                                              gTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACAAATg     >  2:684153/1‑140 (MQ=255)

                                                                                                                              gTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACAAATg     >  2:1250826/1‑140 (MQ=255)

                                                                                                                              gTTGGCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACAAATg     >  1:225009/1‑140 (MQ=255)

                                                                                                                                 ggCAACTGTCGGTCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTGGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGAGCACAAGCGCAGAACAAATGCTg  >  2:3639624/1‑140 (MQ=255)

                                                                                                                                 ggCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAGCATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACAAATGCTg  >  1:400894/1‑140 (MQ=255)

                                                                                                                                 ggCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACAAATGcgg  >  2:2888075/1‑138 (MQ=255)

                                                                                                                                 ggCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACAAATGCTg  >  1:1873990/1‑140 (MQ=255)

                                                                                                                                 ggCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACAAATGCTg  >  1:2518709/1‑140 (MQ=255)

                                                                                                                                 ggCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACAAATGCTg  >  1:258652/1‑140 (MQ=255)

                                                                                                                                 ggCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACAAATGCTg  >  1:3875213/1‑140 (MQ=255)

                                                                                                                                 ggCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACAAATGCTg  >  1:4851242/1‑140 (MQ=255)

                                                                                                                                 ggCAACTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACAAATGCTg  >  2:1412480/1‑140 (MQ=255)

GTACGCTGTTGTCACGTATTACCTACGATTCCGAACAGGTTGCTTCTTCTTCTTCCGGCGCACTGATTACTGTTGTGCGTGAAGGTGCGTCGATCATCGGCCTGTTCATCATGATGTTCTATTACAGTTGGCAGCTGTCGATCATTTTGATTGTGCTGGCACCGATTGTTTCGATTGCGATTCGCGTTGTATCGAAGCGTTTTCGCAACATCAGTAAAAACATGCAGAACACCATGGGGCAGGTGACCACCAGCGCAGAACAAATGCTG  >  NZ_CP025268/974091‑974359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`