BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,013,596	T→C	pseudogene (539/542 nt) 370 (TAA→TAG)	zapC → ycbX ←	cell division protein ZapC 6‑N‑hydroxylaminopurine resistance protein YcbX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,013,596	0	T	C	99.5%	714.5 / NA	219	pseudogene (539/542 nt) 370 (TAA→TAG)	zapC ycbX	cell division protein ZapC 6‑N‑hydroxylaminopurine resistance protein YcbX
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (122/95); minor base A (1/0); total (124/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 219 total aligned reads displayed.

CCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑GTT‑GG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCTAAAAGCTGAACACGGCAACTTCCACAAATGCCCG  >  NZ_CP025268/1013461‑1013732

ccGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTaa                                                                                                                                       <  1:2702454/140‑1 (MQ=255)

 cGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAAc                                                                                                                                      >  1:2304821/1‑140 (MQ=255)

    aCTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAAcgcg                                                                                                                                   <  1:382665/140‑1 (MQ=255)

      tATGCCTGCTGGCACCACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGGCGCCATTAAAATCATGAACGACGGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGcc                                                                                                                                 <  2:3392351/140‑1 (MQ=255)

           ctgctgGCACAAGCCTGCGTTGTTAGTGCGAGTCGCGCGATGCA‑GATGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACtt                                                                                                                            <  2:1931769/140‑1 (MQ=255)

           ctgctgGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACtt                                                                                                                            <  2:1654402/140‑1 (MQ=255)

           ctgcggGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACtt                                                                                                                            <  1:3205632/140‑1 (MQ=255)

             gctgGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTaa                                                                                                                          >  1:4313893/1‑140 (MQ=255)

             gctgGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTaa                                                                                                                          >  2:3994501/1‑140 (MQ=255)

              ctgGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAg                                                                                                                         >  1:3908636/1‑140 (MQ=255)

               tgGCACAACCCTGCGGTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑GGACGCCATTAAAATCATGAACGACAGGCTGATACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGTAGTCGAACGCGCCAACTTAAGt                                                                                                                        >  2:2332768/1‑140 (MQ=255)

                ggCACAACCGTGCGTTGGTATTGCGGGTCTCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAAGGACAGGCTGAAACCGCGGGTTAATGTGGACAGCTTCAGCCTCGAACAGGCAGTCTAAGGCGCCAACGTAAATg                                                                                                                       >  2:423257/1‑140 (MQ=255)

                ggCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTg                                                                                                                       <  2:3466379/140‑1 (MQ=255)

                ggCACAACCCTGCCTCCTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTg                                                                                                                       <  2:2437652/140‑1 (MQ=255)

                  cacaACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAACCCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCa                                                                                                                     <  2:1239928/140‑1 (MQ=255)

                  cacaACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAGTGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCa                                                                                                                     >  1:3652681/1‑140 (MQ=255)

                  cacaACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCa                                                                                                                     <  2:1537018/140‑1 (MQ=255)

                  cacaACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCa                                                                                                                     >  2:1385495/1‑140 (MQ=255)

                  cacaACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCa                                                                                                                     <  1:3972905/140‑1 (MQ=255)

                  cacaACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCa                                                                                                                     >  2:2701881/1‑140 (MQ=255)

                  cacaACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCa                                                                                                                     >  1:2637093/1‑140 (MQ=255)

                  cacaACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCa                                                                                                                     <  2:3041336/140‑1 (MQ=255)

                  cacaACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCa                                                                                                                     <  1:4163000/140‑1 (MQ=255)

                  cacaACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCa                                                                                                                     <  1:57665/140‑1 (MQ=255)

                  cacaACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCAGGAACGACAGGCTGAAACCGCAGGTGAATGTTTACAGCTTGAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGGGCa                                                                                                                     >  1:3122757/1‑140 (MQ=255)

                    caACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGt                                                                                                                   <  1:2077075/140‑1 (MQ=255)

                    caACCCTGCGTTGTTAGTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATAAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTTAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGt                                                                                                                   <  1:2838427/140‑1 (MQ=255)

                      aCCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCt                                                                                                                 <  2:4369469/140‑1 (MQ=255)

                       cccTGCGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCtt                                                                                                                <  1:1874566/140‑1 (MQ=255)

                       cccTGCGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCtt                                                                                                                >  1:344946/1‑140 (MQ=255)

                       cccTGCGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCtt                                                                                                                <  1:406193/140‑1 (MQ=255)

                          tGCGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCGACTTAAGTGCAGTCTTCgg                                                                                                             <  2:2281836/140‑1 (MQ=255)

                            cGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCtt                                                                                                                <  2:3438666/135‑1 (MQ=255)

                            cGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCtt                                                                                                                >  1:3438666/1‑135 (MQ=255)

                            cGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTa                                                                                                           <  1:4668147/140‑1 (MQ=255)

                            cGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTa                                                                                                           >  1:3797929/1‑140 (MQ=255)

                            cGTTGTTATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTa                                                                                                           >  1:1552346/1‑140 (MQ=255)

                            agttgtcATTCCGGGTCGCGGACTGCG‑GTTGGG‑CGCCGCCATTAAGATCATGAACGAGAGGGCGTAACCGAAGGTTAAGATGGACAGCTGCAGCCCCGAACGGGCAGTCTCGCGCGGCGACTTAAGTGCAGTCTTCGGTa                                                                                                           <  2:3861806/139‑1 (MQ=255)

                             gttgttATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTac                                                                                                          <  1:2864940/140‑1 (MQ=255)

                             gttgttATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTac                                                                                                          <  1:1473012/140‑1 (MQ=255)

                                 ttcttGCGGGTGGCGCGGTGAA‑GTT‑GGTCGACGCCATTAAAATCATGCACGACAGCCTGAAAACGCAGGTTAAGGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCCTCTTAAGTGCAGTCTTCGGTACACAg                                                                                                      <  2:1136413/137‑1 (MQ=255)

                                 ttATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAg                                                                                                      >  2:884936/1‑140 (MQ=255)

                                 ttATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAg                                                                                                      >  1:4922582/1‑140 (MQ=255)

                                 ttATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAg                                                                                                      >  1:4763923/1‑140 (MQ=255)

                                 ttATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAg                                                                                                      >  1:616668/1‑140 (MQ=255)

                                 ttATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAg                                                                                                      >  1:2962070/1‑140 (MQ=255)

                                 ttATTGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAg                                                                                                      >  1:3489662/1‑140 (MQ=255)

                                 tgaTTGCGGGTCGCGCGATGCA‑GTTGGG‑CTTCGCCATTAAAGTCATGTCCGACAGGCTTAAACCGCTGGTTCATGTTGACAGCTTCGGGCTTGAACAGGCAGTCTAACGCCCTTACTTAAGTTCTGTCTTCGGTatatgg                                                                                                      >  2:292609/3‑135 (MQ=255)

                                    ttGCGGGTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTa                                                                                                   <  2:4642254/140‑1 (MQ=255)

                                         ggTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCa                                                                                              >  2:2367107/1‑140 (MQ=255)

                                         ggTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCa                                                                                              <  1:257300/140‑1 (MQ=255)

                                         ggTCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCa                                                                                              <  1:2283011/140‑1 (MQ=255)

                                           tCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCaaa                                                                                            <  1:1267892/140‑1 (MQ=255)

                                           tCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCaaa                                                                                            >  2:30790/1‑140 (MQ=255)

                                           tCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCaaa                                                                                            >  1:1843638/1‑140 (MQ=255)

                                           tCGCGCGATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCaaa                                                                                            >  2:4995169/1‑140 (MQ=255)

                                            cgcgcgATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAg                                                                                           <  1:750177/140‑1 (MQ=255)

                                              ggcgATGCAGGTG‑GG‑CGACGCCGTTAACATCATGAGCGACAGGCTGAAACCGCAGGTTAATGTTTACAGCTTCAGCCTCGAAAAGGCAGTCTAACGCGCGAACTTAAGGGCAGTCTTCGGGACACAGCTACAGCAAGGaa                                                                                         >  2:1839009/2‑140 (MQ=255)

                                              cgcgATGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGaa                                                                                         >  1:37648/1‑140 (MQ=255)

                                                   tGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTg                                                                                    <  1:1768547/140‑1 (MQ=255)

                                                   tGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTg                                                                                    <  2:4274654/140‑1 (MQ=255)

                                                   tGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTg                                                                                    >  1:133330/1‑140 (MQ=255)

                                                   tGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTg                                                                                    <  2:2651260/140‑1 (MQ=255)

                                                   tGCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTg                                                                                    <  1:4533215/140‑1 (MQ=255)

                                                    gCA‑GTTGGG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGc                                                                                   <  1:222420/140‑1 (MQ=255)

                                                         ttggg‑CGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCcatc                                                                               >  1:2813246/4‑140 (MQ=255)

                                                               cGACTCCATTAAAATCATGGACGACAGGCGGAAACCGCAGGTTAATGTTGACAGCTTCACCTTCGAACAGGCAGTGCAACGCGCCAACTTAATTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGGGCCAACCTCGc                                                                          >  2:2801577/1‑140 (MQ=255)

                                                               cGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCGGGTTAATGTTGACAGCTGCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCTGTACACAGCTACAGCAAAGAATGGTGCcatcacagg                                                                          >  2:1782410/1‑136 (MQ=255)

                                                               cGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACa                                                                                                 >  1:5050951/1‑117 (MQ=255)

                                                               cGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGc                                                                          >  1:972468/1‑140 (MQ=255)

                                                               cGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGc                                                                          >  2:4877136/1‑140 (MQ=255)

                                                                 aCGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGccc                                                                        >  1:1648479/1‑140 (MQ=255)

                                                                  cGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCa                                                                       <  1:595618/140‑1 (MQ=255)

                                                                               aTGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGGCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATAGCCCATTGCTGAtttttt                                                          >  2:3640712/1‑140 (MQ=255)

                                                                                  aaCGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAg                                                       <  2:1588084/140‑1 (MQ=255)

                                                                                          gCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGa                                               >  1:1001286/1‑140 (MQ=255)

                                                                                          gCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGAAAAGAATGGTGCCATCAGCGCCCATTGCTGATTTTTTCAGCGGCGTGa                                               >  2:832475/1‑140 (MQ=255)

                                                                                                agcGCAGGTTAATGTTGACAGCTTTAGCCTCGGACAGGCAGTCTAACGAGCCAAATTAAGTGCAGTCTTCGGTACACAGCTACAGGAAAGGATGGTGCGATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGc                                         <  2:2675360/138‑1 (MQ=255)

                                                                                                aCCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGc                                         <  1:3297265/140‑1 (MQ=255)

                                                                                                aCCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGc                                         <  2:70549/140‑1 (MQ=255)

                                                                                                    cAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTg                                                                    <  2:1621218/109‑1 (MQ=255)

                                                                                                    cAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTc                                     >  1:2735184/1‑140 (MQ=255)

                                                                                                    cAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTc                                     >  1:4440275/1‑140 (MQ=255)

                                                                                                     aGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCt                                    <  1:3361658/140‑1 (MQ=255)

                                                                                                     aGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCt                                    <  2:3421211/140‑1 (MQ=255)

                                                                                                        ttAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCTaaa                                 >  1:4622974/1‑140 (MQ=255)

                                                                                                          aaTGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCTAAAAg                               <  1:1343792/140‑1 (MQ=255)

                                                                                                               tGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCTAAAAGCTGaa                          <  2:316513/140‑1 (MQ=255)

                                                                                                                 aCAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCTAAAAGCTGAaca                        >  2:333615/1‑140 (MQ=255)

                                                                                                                 aCAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCTAAAAGCTGAaca                        >  2:4632213/1‑140 (MQ=255)

                                                                                                                 aCAGCTTCAGCCGCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCTAAAAGCTGAaca                        >  2:5041671/1‑140 (MQ=255)

                                                                                                                     cTTCAGCCTCGAACAGGCAGTCTCCCGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCACAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCTAAAAGCTGAACACGGc                    >  2:2083896/1‑140 (MQ=255)

                                                                                                                      ttCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCTAAAAGCTGAACACGGCa                   <  1:849771/140‑1 (MQ=255)

                                                                                                                      ttCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCTAAAAGCTGAACACGGCa                   >  1:3249668/1‑140 (MQ=255)

                                                                                                                        cAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCTAAAAGCTGAACACGGCAAc                 >  2:4572503/1‑140 (MQ=255)

                                                                                                                        cAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCTAAAAGCTGAACACGGCAAc                 >  1:2926085/1‑140 (MQ=255)

                                                                                                                          gCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCTAAAAGCTGAACACGGCAACtt               <  2:4922582/140‑1 (MQ=255)

                                                                                                                               gAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCTAAAAGCTGAACACGGCAACTTCcaca          >  2:3237264/1‑140 (MQ=255)

                                                                                                                                aaCAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCTAAAAGCTGAACACGGCAACTTCCACaa         >  2:1050841/1‑140 (MQ=255)

                                                                                                                                       aGTCTAACGCGCCAACTTAAGTGGAGGCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCTAAAAGCTGAACACGGCAACTTCCACAAATGCCcg  <  1:2731493/140‑1 (MQ=255)

CCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑GTT‑GG‑CGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGCCATCATCGCCCATTGCTGATTTTTTCAGCGGCGTGACTTCGCCTTCTAAAAGCTGAACACGGCAACTTCCACAAATGCCCG  >  NZ_CP025268/1013461‑1013732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`