Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 1,211,261 A→G K54E (AAG→GAG)  CXP41_RS06340 → DUF4222 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252681,211,2610AG99.5% 1454.0 / NA 429K54E (AAG→GAG) CXP41_RS06340DUF4222 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (211/216);  minor base T (0/2);  total (211/218)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 429 total aligned reads displayed.

CAAAGATCACTACGGAGTGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACGAAGGTGTGTGCTGGCCATC  >  NZ_CP025268/1211125‑1211395
                                                                                                                                        |                                                                                                                                      
 aaaGATCACTACGGAGTGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAggt                                                                                                                                    <  2:2018191/140‑1 (MQ=255)
   aGATCACTACGGAGTGCCCGGCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAggtcg                                                                                                                                  <  1:579409/140‑1 (MQ=255)
       cACTACGGAGTGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCa                                                                                                                              >  1:2450292/1‑140 (MQ=255)
        ccTACGGAGTGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCAt                                                                                                                             <  2:4372715/139‑1 (MQ=255)
        aCTACGGAGTGGCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGGAATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTGAGCCCGCTCGAACAGTGTCGTCGTAAATTCAGGGAAACAGAGGTCGGTCAt                                                                                                                             >  2:4566765/1‑140 (MQ=255)
        aCTACGGAGTGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCAt                                                                                                                             >  2:3296015/1‑140 (MQ=255)
        aCTACGGAGTGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCAt                                                                                                                             >  2:4492723/1‑140 (MQ=255)
        aCTACGGAGTGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCAt                                                                                                                             <  1:2868113/140‑1 (MQ=255)
            cGGCGTGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTCCCTCCGCGAAGGTTATGAGCATGCATGCTTCAGCCCGCTCGAACAGTCTCGTCGTAAATTCAGGGCAATAGAGGTCGGTCATGAGc                                                                                                                         <  1:1832690/140‑1 (MQ=255)
            cGGAGTGCTCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGATCGAACAGTTTCGTCGTAAAGTCAGGGAAATAGAGGTCGGTCATGAGc                                                                                                                         <  2:3739143/140‑1 (MQ=255)
            cGGAGTGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGc                                                                                                                         <  2:2431156/140‑1 (MQ=255)
            cGGAGTGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGc                                                                                                                         <  2:4613192/140‑1 (MQ=255)
             ggAGTGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCa                                                                                                                        <  1:2589909/140‑1 (MQ=255)
               aGTGCCCTTCAGAGTCATTCGTTGGGAGCCGGAAACACAGCGGGTTAACTAGCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTGAATTCAGGGAAATAGAGGTCGGTCAGGAGCACt                                                                                                                      >  2:4051879/1‑140 (MQ=255)
               aGTGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAGATTCAGGGAAATAGAGGTCGGTCATGAGCACt                                                                                                                      >  2:4231117/1‑140 (MQ=255)
               aGTGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACt                                                                                                                      >  1:2368808/1‑140 (MQ=255)
                 tGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTaa                                                                                                                    >  2:4135260/1‑140 (MQ=255)
                 tGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTaa                                                                                                                    >  2:2544645/1‑140 (MQ=255)
                 tGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTaa                                                                                                                    >  2:4994144/1‑140 (MQ=255)
                 tGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTaa                                                                                                                    >  1:1172635/1‑140 (MQ=255)
                 tGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTGTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTaa                                                                                                                    >  1:4014352/1‑140 (MQ=255)
                    ccGTCAGAGTCATTCGGTGGGAGCCGGAAACACAGCGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACATTTTCGGCGTAAAGTCAGGGAAATAGAGGACGTTTATGAGCACTAAAtt                                                                                                                 >  2:4848460/1‑140 (MQ=255)
                      gTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTaa                                                                                                               >  2:3412192/1‑140 (MQ=255)
                       tCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAAc                                                                                                              <  2:3554488/140‑1 (MQ=255)
                       tCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAAc                                                                                                              <  1:338401/140‑1 (MQ=255)
                       tCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAAc                                                                                                              <  1:3744761/140‑1 (MQ=255)
                       tCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAAc                                                                                                              <  1:4970819/140‑1 (MQ=255)
                       tCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAAc                                                                                                              <  1:1694718/140‑1 (MQ=255)
                       tCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAAc                                                                                                              <  1:1345297/140‑1 (MQ=255)
                          gcgTCATTCGTTATGAGCCCGAAACCCAACAGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCCCTCGAACAGTTTCGTCGTAAATTCAGTAAAATAGAGGTCGGTCATGAGCCCTAAATTAACCgg                                                                                                           <  2:1240219/138‑1 (MQ=255)
                          gagTCCTTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCgg                                                                                                           <  1:693801/140‑1 (MQ=255)
                          gagTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCgg                                                                                                           <  1:2164068/140‑1 (MQ=255)
                          gagTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCgg                                                                                                           <  1:1871527/140‑1 (MQ=255)
                          gagTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCgg                                                                                                           <  2:2311035/140‑1 (MQ=255)
                           agTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGc                                                                                                          >  1:4410407/1‑140 (MQ=255)
                           agTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGc                                                                                                          >  1:3568592/1‑140 (MQ=255)
                           agTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGc                                                                                                          <  2:581739/140‑1 (MQ=255)
                            gTCATTCGTTGGGCGCCGGAACCACAACGGGTTACCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCt                                                                                                         <  1:2777750/140‑1 (MQ=255)
                            gTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCt                                                                                                         <  1:862510/140‑1 (MQ=255)
                            gTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCt                                                                                                         <  1:4293203/140‑1 (MQ=255)
                            gTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCt                                                                                                         <  2:1700778/140‑1 (MQ=255)
                             tCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGGAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCta                                                                                                        >  2:259288/1‑140 (MQ=255)
                                ttCGTTGGGAGCCGGAAACACAACGGGTTAGCTACCTCCGCGAAGGGTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGATCACTAAATTAACCGGCtatgt                                                                                                     >  2:2701995/1‑140 (MQ=255)
                                         aGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATgg                                                                                            <  1:19675/140‑1 (MQ=255)
                                             ggAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTgc                                                                                        <  1:1582643/140‑1 (MQ=255)
                                              gAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTgcg                                                                                       <  1:978214/140‑1 (MQ=255)
                                              gAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTgcg                                                                                       <  2:510989/140‑1 (MQ=255)
                                                 acacCACGGGTTACCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCACGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTg                                                                                    <  2:1070340/140‑1 (MQ=255)
                                                    caACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCAt                                                                                 <  1:5106832/140‑1 (MQ=255)
                                                     aaccagTTATCTACCTCCGCGAAGGTTAAGAGCATGACTGCTCCAGCCCGCTCGACCAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGCTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATc                                                                                <  2:660567/135‑1 (MQ=255)
                                                     aaCGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATc                                                                                <  2:1576525/140‑1 (MQ=255)
                                                        gggTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAgg                                                                             >  2:2048707/1‑140 (MQ=255)
                                                         ggTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTTGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGc                                                                            <  1:4262424/140‑1 (MQ=255)
                                                          gTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACTGTTCCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCa                                                                           <  1:3638285/140‑1 (MQ=255)
                                                          gTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCa                                                                           <  1:2093631/140‑1 (MQ=255)
                                                                   cTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTAt                                                                  >  1:921351/1‑140 (MQ=255)
                                                                    tCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATc                                                                 >  1:2997780/1‑140 (MQ=255)
                                                                    tCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATc                                                                 >  2:1354807/1‑140 (MQ=255)
                                                                     ccGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTCACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATcc                                                                >  2:848866/1‑140 (MQ=255)
                                                                     ccGCGAAGGTTATCAGCATGAATGCTTCCGCCCGCTCGAACCGTTTCGTCGTAAATTTAGGGAAATAGAGGTCGGTCATGAGCACGAAATTAACCGGCTATGTATGGGCTGGTTGCTCTGCATCAGGCATGAAGTTATcc                                                                >  2:67981/1‑140 (MQ=255)
                                                                        cgAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGc                                                             >  1:2054196/1‑140 (MQ=255)
                                                                        cgAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGc                                                             >  2:2177299/1‑140 (MQ=255)
                                                                        cgAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGc                                                             >  1:311479/1‑140 (MQ=255)
                                                                        cgAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTGTCGTCGTAGATTCACGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGGTATGTATGGGATGGTTGCGCTGCATCAGGCATGAATTTATCCAGc                                                             >  2:4550011/1‑140 (MQ=255)
                                                                          aaGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCAGGAAGTTATCCAGCGt                                                           >  2:1809536/1‑140 (MQ=255)
                                                                            ggTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATTGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTgg                                                         <  2:4880271/140‑1 (MQ=255)
                                                                            ggTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTgg                                                         <  1:4607808/140‑1 (MQ=255)
                                                                            ggTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCAGCAGGCATGAAGTTATCCAGCGTgg                                                         <  1:4514795/140‑1 (MQ=255)
                                                                             gTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGc                                                        >  1:2895971/1‑140 (MQ=255)
                                                                                     cATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGAATGGTTGCGCTGCATCAGGCATGAAGTTATACAGCGTGGCAATTATgg                                                >  2:3825447/1‑140 (MQ=255)
                                                                                       tGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGcc                                              >  1:4841374/1‑140 (MQ=255)
                                                                                       tGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGcc                                              >  1:4229275/1‑140 (MQ=255)
                                                                                           tGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGcc                                          <  1:753484/140‑1 (MQ=255)
                                                                                             cTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTg                                        >  1:3780074/1‑140 (MQ=255)
                                                                                             cTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTg                                        >  2:3622106/1‑140 (MQ=255)
                                                                                             cTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTg                                        >  1:1265733/1‑140 (MQ=255)
                                                                                             cTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTg                                        >  1:3053852/1‑140 (MQ=255)
                                                                                             cTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTg                                        >  1:1542379/1‑140 (MQ=255)
                                                                                               tCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGc                                      >  1:230370/1‑140 (MQ=255)
                                                                                               tCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGc                                      >  1:3254206/1‑140 (MQ=255)
                                                                                                cAGCCCGCTCGAACAGTGTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTGGCGCTGCAGCAGGCATGAAGTTATCCATCGTGGCAATTATGGCCCGCCTGGCt                                     >  2:1484424/1‑140 (MQ=255)
                                                                                                cAGCCCCCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCt                                     <  2:795585/140‑1 (MQ=255)
                                                                                                  gCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGc                                                             <  2:2651819/114‑1 (MQ=255)
                                                                                                  gCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGa                                   >  1:1005439/1‑140 (MQ=255)
                                                                                                  gCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGa                                   >  2:2134857/1‑140 (MQ=255)
                                                                                                       cTCGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCa                              >  1:4437331/1‑140 (MQ=255)
                                                                                                         cGAACAGTTTCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGt                            <  2:3955017/140‑1 (MQ=255)
                                                                                                                tttCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACg                     <  1:3412192/140‑1 (MQ=255)
                                                                                                                tttCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACg                     <  2:3143430/140‑1 (MQ=255)
                                                                                                                tttCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACg                     <  2:2866/140‑1 (MQ=255)
                                                                                                                tttCGTCGTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTCGCTGATTTCAGTAATGACg                     <  2:2980575/140‑1 (MQ=255)
                                                                                                                  tcgtcgTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACGaa                   >  1:3987125/1‑140 (MQ=255)
                                                                                                                  tcgtcgTAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACGaa                   >  2:3526286/1‑140 (MQ=255)
                                                                                                                   cgtcgtAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACGAAg                  <  2:3691523/140‑1 (MQ=255)
                                                                                                                    gtcgtAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACGAAgg                 <  2:1342994/140‑1 (MQ=255)
                                                                                                                     tcgtAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACGAAGgt                >  1:4713791/1‑140 (MQ=255)
                                                                                                                     tcgtAAATTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACGAAGgt                <  2:1978674/140‑1 (MQ=255)
                                                                                                                          aaTTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACGAAGGtgtgtg           >  1:2414333/1‑140 (MQ=255)
                                                                                                                          aaTTCAGGGAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACGAAGGtgtgtg           >  1:105441/1‑140 (MQ=255)
                                                                                                                                  gAAATAGAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACGAAGGTGTGTGCTGGCCAt   <  1:403838/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
CAAAGATCACTACGGAGTGCCCGTCAGAGTCATTCGTTGGGAGCCGGAAACACAACGGGTTATCTACCTCCGCGAAGGTTATGAGCATGAATGCTTCAGCCCGCTCGAACAGTTTCGTCGTAAATTCAGGGAAATAAAGGTCGGTCATGAGCACTAAATTAACCGGCTATGTATGGGATGGTTGCGCTGCATCAGGCATGAAGTTATCCAGCGTGGCAATTATGGCCCGCCTGGCTGATTTCAGTAATGACGAAGGTGTGTGCTGGCCATC  >  NZ_CP025268/1211125‑1211395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑