Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,219,803	T→C	*135* (TAA→TAG)	ycgX ←	DUF1398 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,219,803	0	T	C	100.0%	198.5 / NA	60	*135* (TAA→TAG)	ycgX	DUF1398 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/16);  total (44/16)

TTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGT  >  NZ_CP025268/1219668‑1219936                                                                                                                                        |                                                                                                                                      ttAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGa                                                                                                                                   >  1:3008814/1‑140 (MQ=255)     tCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGGTAATGCCTTACTGAATTATGAACCATTTCTTAAGTATCCAACTTTAGCTAGATTaa                                                                                                                               >  2:4812734/1‑140 (MQ=255)     tCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTaa                                                                                                                               <  2:133297/140‑1 (MQ=255)         gTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTAGTGAATTGTGAAGCATTTCTTAAGTATCTAACTTTAGCTAGATTAATGGt                                                                                                                           >  2:4490329/1‑140 (MQ=255)         gTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGt                                                                                                                           >  1:512706/1‑140 (MQ=255)         gTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGt                                                                                                                           <  1:515249/140‑1 (MQ=255)         gTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGt                                                                                                                           >  2:2399912/1‑140 (MQ=255)         gTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGt                                                                                                                           >  1:2874347/1‑140 (MQ=255)         gTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGt                                                                                                                           >  1:2874348/1‑140 (MQ=255)                   atGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTAtttt                                                                                                                 >  1:2859086/1‑140 (MQ=255)                     gggTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCt                                                                                                               <  2:3008814/140‑1 (MQ=255)                      gttGACAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTa                                                                                                              >  1:616276/3‑140 (MQ=255)                      ggTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTa                                                                                                              >  2:4473307/1‑140 (MQ=255)                          aCAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATc                                                                                                          >  1:4660893/1‑140 (MQ=255)                              tAAAAAGACTGTATTAATCATACATAGAGATAGTCTCTGCTCTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGGTTATTATTTTCTACATCTTCa                                                                                                      <  2:2314872/140‑1 (MQ=255)                              tAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCa                                                                                                      <  2:4248275/140‑1 (MQ=255)                                        gTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCAATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATaaaa                                                                                            >  2:4741227/1‑140 (MQ=255)                                         tATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAg                                                                                           >  2:1000786/1‑140 (MQ=255)                                         tATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAg                                                                                           >  1:1621448/1‑140 (MQ=255)                                         tATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAg                                                                                           >  1:1172844/1‑140 (MQ=255)                                                   ccATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCACTATATAAAAGCGTATTATCa                                                                                 <  2:2003652/140‑1 (MQ=255)                                                   ccATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCa                                                                                 >  2:4888445/1‑140 (MQ=255)                                                   ccATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCa                                                                                 >  1:4824708/1‑140 (MQ=255)                                                   ccATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCa                                                                                 >  1:1300943/1‑140 (MQ=255)                                                   ccATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCa                                                                                 >  1:1377168/1‑140 (MQ=255)                                                   ccATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCa                                                                                 >  1:18019/1‑140 (MQ=255)                                                   ccATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCa                                                                                 <  1:236757/140‑1 (MQ=255)                                                   ccATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCa                                                                                 >  1:3490787/1‑140 (MQ=255)                                                           aTAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATgg                                                                             <  1:3881050/136‑1 (MQ=255)                                                           aTAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATgg                                                                             >  2:3881050/1‑136 (MQ=255)                                                             aGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCgtagt                                                                       >  1:3301412/1‑140 (MQ=255)                                                             aGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTAAATATTCAATATATAAAAGCGTATTATCAATGGCgtagt                                                                       >  2:574552/1‑140 (MQ=255)                                                              gTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCgtagta                                                                      >  2:2268495/1‑140 (MQ=255)                                                                ctctGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAAc                                                                    <  2:3301412/140‑1 (MQ=255)                                                                        ttttATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTg                                                            >  1:599858/1‑140 (MQ=255)                                                                         tttATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGt                                                           <  1:4937996/140‑1 (MQ=255)                                                                             tttCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATg                                                       >  1:4714318/1‑140 (MQ=255)                                                                                 cATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTa                                                   <  1:322063/140‑1 (MQ=255)                                                                                    tATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACa                                                >  1:3533475/1‑140 (MQ=255)                                                                                      tGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATc                                              <  1:2399912/140‑1 (MQ=255)                                                                                            tgtcTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAAc                                        >  2:2721702/4‑140 (MQ=255)                                                                                                ttACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCAc                                    >  2:4148008/1‑140 (MQ=255)                                                                                                ttACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCAc                                    >  2:4148177/1‑140 (MQ=255)                                                                                                 tACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCAcc                                   >  1:3875915/1‑140 (MQ=255)                                                                                                  aCTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCATCg                                  >  1:4383592/1‑140 (MQ=255)                                                                                                  aCTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCg                                  >  1:4611555/1‑140 (MQ=255)                                                                                                  aCTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCg                                  >  1:4440171/1‑140 (MQ=255)                                                                                                        ttATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACAt                                                                                                           <  2:1597628/61‑1 (MQ=255)                                                                                                        ttATGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACAt                                                                                                           >  1:1597628/1‑61 (MQ=255)                                                                                                           tGAAGCATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGcc                         >  1:2195082/1‑140 (MQ=255)                                                                                                                cATTTCTTAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCgcgc                    >  2:2819462/1‑140 (MQ=255)                                                                                                                  tttCTTCAGTATCCAAATTTAGCAAGGTTAAGGGTATATTATTATCTACATCATCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTAATGATTATCCTCAGTAACACACCGGAACACGTCCGCGCCt                  >  2:1056141/1‑140 (MQ=255)                                                                                                                      ttAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCa              <  2:2682853/140‑1 (MQ=255)                                                                                                                      ttAAGTATCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCa              <  2:1882198/140‑1 (MQ=255)                                                                                                                             tCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTGa       >  2:3090169/1‑140 (MQ=255)                                                                                                                             tCCAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTGa       >  2:1500704/1‑140 (MQ=255)                                                                                                                             tCCAACTTTAGCTAGATTAATGGTTTATTAGTTTCTGCATCTTCAATATATAAAAGCGTTTTATCAATGGCGGAGTAACTGCGTTTGTTGTGATTAACATCAGTAACCCACCGGAAAGCGCCGGCGCCTGCCAGTGTTgt       >  2:4629996/1‑139 (MQ=255)                                                                                                                              ccAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAATATCAGTAACCCACCGGAAAACGCCAGCGCCTGCCAGTGTTGaa      <  2:1997150/140‑1 (MQ=255)                                                                                                                               cAACTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTGAAc     >  1:420424/1‑140 (MQ=255)                                                                                                                                  cTTTAGCTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCTGGCCAGTGTTGAACAGt  >  2:283839/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      TTAGTCTGGTGATTATTTATGGGTGACAATAAAAAGACAGTATTAATCATCCATAGAGATAGTCTCTGCACTTTTATTTCCATTATGCTAATGCCTTACTGAATTATGAAGCATTTCTTAAGTATCCAACTTTAGTTAGATTAATGGTTTATTATTTTCTACATCTTCAATATATAAAAGCGTATTATCAATGGCGTAGTAACTGCGTTTGTTATGATTAACATCAGTAACCCACCGGAAAACGCCCGCGCCTGCCAGTGTTGAACAGT  >  NZ_CP025268/1219668‑1219936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑