BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,293,160	G→A	noncoding (55/132 nt)	CXP41_RS06840 ←	RtT sRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,293,160	0	G	A	91.0%	403.7 / 3.5	152	noncoding (55/132 nt)	CXP41_RS06840	RtT sRNA
Reads supporting (aligned to +/- strand): ref base G (12/1); new base A (95/37); total (112/40)
Fisher's exact test for biased strand distribution p-value = 1.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 153 total aligned reads displayed.

TTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGA  >  NZ_CP025268/1293072‑1293299

                              aCTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGTTGGAGAGtt                                                            >  2:958082/1‑140 (MQ=11)

                              aCTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCCCGAGGCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCACGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGtt                                                            >  2:1252687/1‑140 (MQ=11)

                               cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGGGAGTTc                                                           >  1:1136236/1‑140 (MQ=255)

                                  cccgcTGCGCCCTTCGCCCATCAGATAGCTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAgg                                                        <  2:3532440/138‑1 (MQ=11)

                                  aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAgg                                                        >  1:2369625/1‑140 (MQ=255)

                                   gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCGTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAggg                                                       <  1:4142069/140‑1 (MQ=255)

                                   gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAggg                                                       <  2:922685/140‑1 (MQ=255)

                                   gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAggg                                                       <  1:3230591/140‑1 (MQ=255)

                                   gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAggg                                                       <  2:3694072/140‑1 (MQ=255)

                                   gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAggg                                                       <  1:642592/140‑1 (MQ=255)

                                    cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGCCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCTGGGGCAGAATATTCGATTGCGGATTCGCTAGCCAGTTCAGGGa                                                      >  2:4744049/1‑140 (MQ=11)

                                    cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAgggg                                                      >  1:1795174/1‑139 (MQ=19)

                                    cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                                      >  1:4174209/1‑140 (MQ=255)

                                    cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                                      >  1:2615580/1‑140 (MQ=255)

                                    cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                                      >  1:4913038/1‑140 (MQ=255)

                                    cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                                      >  1:2471327/1‑140 (MQ=255)

                                    cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                                      >  1:827947/1‑140 (MQ=255)

                                    cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                                      >  2:3337823/1‑140 (MQ=255)

                                    cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                                      >  2:4744152/1‑140 (MQ=255)

                                    cgcTGCGCGCTTCGCCCTTCGGGGCGTTGCATGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                                      >  1:3066908/1‑140 (MQ=11)

                                         cgcgcTTCGCCCTTCGGGTCGTTTCCTGCGGCAACGCTCTCTCGCTGACGACCAATCCGAACCTTAGTCGAAGCTTCTCATACTTCCCCGCCTGGGCCGAATATTTAATTGCTGATTCGCTTGAGAGGTCAgggcatgtt                                                 >  2:5090689/1‑134 (MQ=11)

                                         cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAGTTCAGGGGCtttt                                                 >  2:2259071/1‑140 (MQ=11)

                                         cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGGCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                                 >  1:1093662/1‑140 (MQ=255)

                                         cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGGGAGTTCAGGGACtttt                                                 >  2:239384/1‑140 (MQ=255)

                                         cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                                 >  2:1142979/1‑140 (MQ=255)

                                         cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                                 >  1:2183991/1‑140 (MQ=255)

                                         cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                                 <  1:4895378/140‑1 (MQ=255)

                                         cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                                 >  1:4757599/1‑140 (MQ=255)

                                         cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                                 >  1:147031/1‑140 (MQ=255)

                                         cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                                 >  1:439012/1‑140 (MQ=255)

                                         cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                                 >  2:3949581/1‑140 (MQ=255)

                                         cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGAATGCGGACTAGCTTGAGAGTTCAGCGACtttt                                                 >  2:2835810/1‑140 (MQ=11)

                                         cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCATCCCCGCCTGGGGAGAATATTTAATTGCTGATTCGATTGAGCGTTCAGGGACtttt                                                 >  2:4345763/1‑140 (MQ=11)

                                         cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTGCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGCGAGTTCAGGGACtttt                                                 >  2:267967/1‑140 (MQ=255)

                                           cgcTTCCCCCTTCGGGTCGTTGCCTGCGGCAACGCGCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGGGAGTTCAGGGACTTTTGa                                               <  2:2964639/140‑1 (MQ=11)

                                            gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaa                                              <  2:3212510/140‑1 (MQ=255)

                                             cTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaaa                                             <  1:370717/140‑1 (MQ=255)

                                              ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAg                                            >  1:3377661/1‑140 (MQ=255)

                                               tCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGCCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGt                                           <  2:875248/140‑1 (MQ=255)

                                                 gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTga                                         >  1:3813419‑M2/1‑138 (MQ=255)

                                                 gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTga                                         >  2:4570437‑M2/1‑138 (MQ=255)

                                                 gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTga                                         >  2:4570543‑M2/1‑138 (MQ=255)

                                                 gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTga                                         >  2:4408686‑M2/1‑138 (MQ=255)

                                                 gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTga                                         >  1:4175357‑M2/1‑138 (MQ=255)

                                                 gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTga                                         >  1:4146158‑M2/1‑138 (MQ=255)

                                                   cgttcgGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGTGACGAACCTTAGTCCAAGCTTATCATCCTTCCCCGCATGGGCAGAATATTTGAATGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatg                                       >  2:4261934‑M2/3‑136 (MQ=255)

                                                       cGGGGCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTGGTCGAAGTTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtgg                                   >  2:4287971‑M2/1‑132 (MQ=255)

                                                       agggTCGTTGCCTGCGGCGACGCTCGCTCGCTGACGCTCGAGCCGAGCCTTGGTCGAAGCTTCTCAGCCTTCCCCGCGGGGGCAGACTGTTTGATTGCGGATTCGCTTGAGGGTTCGGGGACTTTCGCACGTGAtggtgg                                   <  2:619354/139‑1 (MQ=11)

                                                        gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggt                                  >  2:4118932‑M2/1‑131 (MQ=255)

                                                        gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggt                                  >  1:2533557‑M2/1‑131 (MQ=255)

                                                        gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggt                                  >  1:3862608‑M2/1‑131 (MQ=255)

                                                        gggTCGTTGCCTGCGGCAACGCTCTCTCGCGGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggt                                  >  2:139049‑M2/1‑131 (MQ=255)

                                                        gggTCGTCGCCTGCGGCAACGCTCTCTCGCTGCCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGGCAGTgatggtggt                                  >  2:1098527‑M2/1‑131 (MQ=255)

                                                           tCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggg                               >  1:2220476‑M2/1‑128 (MQ=255)

                                                             gTTGCCTGCGGCCACGCTCTCTCTCTGAGGCTCAGGCCGAACCTTAGTCGAAGCGTCTCATCCTTCACCGCGTGGGCAGAATATTTGATTGCGGATGCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                             >  2:158792‑M2/1‑126 (MQ=255)

                                                             gTTGCCTGCGGCAACGCTTTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                             >  1:995210‑M2/1‑126 (MQ=255)

                                                             gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                             >  2:3601852‑M2/1‑126 (MQ=255)

                                                             gTTGCCTGCGGCAACGCTCTCTCGCTGCCGCTCGAGCCGAACCTTAGTCGAAGTTTCTCATCCTTCCCCGCATTGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                             >  2:5016702‑M2/1‑126 (MQ=255)

                                                             gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                             >  1:2326035‑M2/1‑126 (MQ=255)

                                                             gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                             >  1:995371‑M2/1‑126 (MQ=255)

                                                             gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                             >  1:817174‑M2/1‑126 (MQ=255)

                                                             gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                             >  1:3574158‑M2/1‑126 (MQ=255)

                                                             gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                             >  1:2747102‑M2/1‑126 (MQ=255)

                                                             gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                             >  1:2732246‑M2/1‑126 (MQ=255)

                                                             gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                             >  1:4803074‑M2/1‑126 (MQ=255)

                                                             gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                             >  1:1444271‑M2/1‑126 (MQ=255)

                                                             gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                             >  1:4464650‑M2/1‑126 (MQ=255)

                                                             gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                             >  1:4428537‑M2/1‑126 (MQ=255)

                                                             gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCACCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGGAAGTgatggtggtggggg                             >  2:529448‑M2/1‑126 (MQ=255)

                                                             gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGACTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                             >  1:3048023‑M2/1‑126 (MQ=255)

                                                             gTTGCCTGCGGCAACGCCTGCTCGCTGCCGCTCGCGACGAACCTTAGTCGAAGCTTATCAACCCTCCCCGCGTGGGCAGAATACTAGATTGCGGATTCGCTTCAGAGTTCAGGGACTTTTGAACGCGATGGTGGTggggg                             <  2:1327995/140‑1 (MQ=255)

                                                               tGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTTCGGATTCGCTGGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaa                           <  2:1940975‑M2/140‑17 (MQ=255)

                                                                gcgtGCGGCGACGCTCTCTCGCTGACGATCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAACGTGATGGTGGTGGGGGAAg                          <  2:14144/137‑1 (MQ=14)

                                                                  cTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTGGGTCGAAGCTTCTCATCCTGCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                        >  1:1622504‑M2/1‑121 (MQ=255)

                                                                  cTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                        >  1:3690570‑M2/1‑121 (MQ=255)

                                                                  cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                        >  1:1596794‑M2/1‑121 (MQ=255)

                                                                  cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                        >  1:1025337‑M2/1‑121 (MQ=255)

                                                                  cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                        >  2:4047003‑M2/1‑121 (MQ=255)

                                                                  cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                        >  1:128890‑M2/1‑121 (MQ=255)

                                                                  cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                        >  1:2790098‑M2/1‑121 (MQ=255)

                                                                  cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                        >  1:4541026‑M2/1‑121 (MQ=255)

                                                                  cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                        >  1:4651218‑M2/1‑121 (MQ=255)

                                                                  cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                        >  1:4858327‑M2/1‑121 (MQ=255)

                                                                  cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGGTCAGGGACTTTTGAAAGTgatgggggtgggggaagga                        >  1:3588647‑M2/1‑121 (MQ=255)

                                                                   tGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggat                       <  2:68295‑M2/140‑21 (MQ=255)

                                                                    gCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggatt                      <  1:3031508‑M2/140‑22 (MQ=255)

                                                                    gCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggatt                      <  1:463253‑M2/140‑22 (MQ=255)

                                                                     cGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggatta                     <  1:932316‑M2/140‑23 (MQ=255)

                                                                       gCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattact                   >  1:2035144‑M2/1‑116 (MQ=255)

                                                                       gCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattact                   >  2:827646‑M2/1‑116 (MQ=255)

                                                                         aaCGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactca                 >  1:2087352‑M2/1‑114 (MQ=255)

                                                                           cGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagc               <  1:4325489‑M2/140‑29 (MQ=255)

                                                                           cGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagc               <  1:3865717‑M2/140‑29 (MQ=255)

                                                                             ctctctCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgc             <  1:4962899‑M2/140‑31 (MQ=255)

                                                                             ctctctCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactaagtgc             <  1:184839‑M2/140‑31 (MQ=255)

                                                                                tctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgc          <  2:3468833‑M2/140‑34 (MQ=255)

                                                                                tctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCAGCCTTCACCGCATGGGCAGAATATGTGATTGCGGATTCGCTTGAGAGTTCAGGGAGTTTTGGAAGTgatggtggtgggggaaggattactcagcgctgc          >  2:3002586‑M2/1‑107 (MQ=255)

                                                                                      tGGCGCGCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCGTGGGCAGAATATTTGACGGCGGATTCGCTTGGGAGTTCCGGGTCTATTGAAGGGgatggtggtgggggaaggattactcagcgctgcgcgctt    >  2:2834977‑M2/1‑101 (MQ=255)

                                                                                      tGCCGCTCGAGCCGAACCTTAGTCGAAGTTTATCATCATTCACCGCATGGGCAGAATATTTGATTGCGGAGTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcgcgctt    >  2:2033742‑M2/1‑101 (MQ=255)

                                                                                        gcgcTCGAGCCGAAACTTTGTCGAAGCTTATCATCCTTCCACGCGTGGGCAGAATATTTGATGGCGGATTCGGTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcgcgcttcg  >  2:1622626‑M2/1‑99 (MQ=255)

TTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGA  >  NZ_CP025268/1293072‑1293299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`