BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,394,060	T→C	pseudogene (313/966 nt)	ycjG →	L‑Ala‑D/L‑Glu epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,394,060	0	T	C	99.7%	1112.9 / NA	332	pseudogene (313/966 nt)	ycjG	L‑Ala‑D/L‑Glu epimerase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (151/180); minor base A (1/0); total (152/180)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 333 total aligned reads displayed.

GGCGCAAATTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGCGCGAAATTACTGAAAG  >  NZ_CP025268/1393924‑1394195

    cAAATTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCgct                                                                                                                                  >  1:4675078/1‑140 (MQ=255)

      aaTTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATCCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGCTCTGGCCGCGCGAAGACAGCAGCAATCgctgg                                                                                                                                <  2:1470601/140‑1 (MQ=255)

                gtcgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGAt                                                                                                                      <  2:4906662/140‑1 (MQ=255)

                gtcgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGAt                                                                                                                      <  1:1444251/140‑1 (MQ=255)

                gtcgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGAt                                                                                                                      <  1:363044/140‑1 (MQ=255)

                gtcgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGAt                                                                                                                      <  2:378326/140‑1 (MQ=255)

                 tcgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGAGCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATc                                                                                                                     >  1:3385411/1‑140 (MQ=255)

                  cgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCg                                                                                                                    >  1:59500/1‑140 (MQ=255)

                   gtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCTCGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCgg                                                                                                                   >  1:4056150/1‑140 (MQ=255)

                        gcgaCTAGGGAAATGTCTGAAGCGGGAGGAGTTGCAAAATATCCTCACTGCCGGCGCAGGACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCAGCGAGAAGACAGCAGCAATCGCTGGCTGATTTGATCTGCATaa                                                                                                              <  2:4301297/137‑1 (MQ=255)

                        gCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATaa                                                                                                              <  1:4551043/140‑1 (MQ=255)

                          aaCTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACg                                                                                                            <  2:544764/140‑1 (MQ=255)

                          aaCTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACg                                                                                                            <  1:985256/140‑1 (MQ=255)

                          aaCTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACg                                                                                                            <  2:3066494/140‑1 (MQ=255)

                          aaCTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACg                                                                                                            <  2:4014552/140‑1 (MQ=255)

                            cTAGAGAAAGGGCTGAGACGGGTGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGCAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGCAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCt                                                                                                          <  1:2831344/140‑1 (MQ=255)

                                 gaAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCg                                                                                                     <  2:106951/140‑1 (MQ=255)

                                   aaGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTACTGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCgag                                                                                                   <  2:3654486/140‑1 (MQ=255)

                                    aGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCgaga                                                                                                  >  2:3855810/1‑140 (MQ=255)

                                    aGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCgaga                                                                                                  <  1:1151649/140‑1 (MQ=255)

                                    aGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCgaga                                                                                                  >  1:1031954/1‑140 (MQ=255)

                                      ggCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGGAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACa                                                                                                >  2:2125213/1‑140 (MQ=255)

                                        cTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGt                                                                                              <  2:59500/140‑1 (MQ=255)

                                        cTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGt                                                                                              <  1:2726613/140‑1 (MQ=255)

                                          gACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGCCAGtga                                                                                            >  2:1734631/1‑138 (MQ=255)

                                           acacGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTAt                                                                                           >  2:2906743/1‑140 (MQ=255)

                                           acacGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTAt                                                                                           <  1:3584074/140‑1 (MQ=255)

                                           acacGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTAt                                                                                           <  1:5086062/140‑1 (MQ=255)

                                           acacGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTAt                                                                                           <  1:2269924/140‑1 (MQ=255)

                                           acacGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTAt                                                                                           <  2:3484435/140‑1 (MQ=255)

                                             acGGGAGGAGTTGCAAGAAATTCTCCCTTCCGGCGCAGCATGTAATGCGCTGGATTGTGCATTGTGGGATCTGACCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATACCGCTTCCCGAGACAGTTATcg                                                                                         <  2:4245787/140‑2 (MQ=255)

                                             acGGGAGGAGTTGCAAAAAATTCTCTCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCa                                                                                         <  2:4621209/140‑1 (MQ=255)

                                             acGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCa                                                                                         <  1:4118424/140‑1 (MQ=255)

                                                     agTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAg                                                                                 >  1:216276/1‑140 (MQ=255)

                                                      gTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGa                                                                                >  1:2577202/1‑140 (MQ=255)

                                                      gTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGa                                                                                <  1:1226596/140‑1 (MQ=255)

                                                      gTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGa                                                                                <  1:2360726/140‑1 (MQ=255)

                                                       ttGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGAc                                                                               >  2:2206400/1‑140 (MQ=255)

                                                        tGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACg                                                                              <  1:3070615/140‑1 (MQ=255)

                                                        tGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACg                                                                              <  2:3885724/140‑1 (MQ=255)

                                                         gCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACgg                                                                             >  1:188456/1‑140 (MQ=255)

                                                           aaaaaaTTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGtt                                                                           <  1:2122850/140‑1 (MQ=255)

                                                              aaaTTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTc                                                                        <  2:3370965/140‑1 (MQ=255)

                                                              aaaTTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTc                                                                        <  1:4045902/140‑1 (MQ=255)

                                                               aaTTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCa                                                                       >  2:736173/1‑140 (MQ=255)

                                                                aTTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCAt                                                                      <  2:4407955/140‑1 (MQ=255)

                                                                   ctcCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCgg                                                                   <  1:4184325/140‑1 (MQ=255)

                                                                     cccTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTTGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTAGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTGGTCATCGGTa                                                                 >  2:3753517/1‑140 (MQ=255)

                                                                     cccTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTa                                                                 <  2:1584084/140‑1 (MQ=255)

                                                                           cGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTg                                                           <  2:1303569/140‑1 (MQ=255)

                                                                              cgcAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATc                                                        <  1:2988547/140‑1 (MQ=255)

                                                                              cgcAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATc                                                        <  2:4756891/140‑1 (MQ=255)

                                                                                cagcaCGTAATGCGCTGGATTGTGGATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                                      <  1:1569988/140‑1 (MQ=255)

                                                                                cagcaCGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                                      <  1:3552110/140‑1 (MQ=255)

                                                                                cagcaCGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                                      <  1:3844142/140‑1 (MQ=255)

                                                                                cagcaCGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                                      <  2:4816723/140‑1 (MQ=255)

                                                                                cagcaCGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                                      <  2:3097467/140‑1 (MQ=255)

                                                                                cagcaCGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                                      <  1:2408740/140‑1 (MQ=255)

                                                                                     cGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGc                                                 <  1:5041535/140‑1 (MQ=255)

                                                                                         aTGCGCTGGATTGTGCATTGTCGGATCTCTCCACGCGAAGACAGCCGCAATCGCTGTATGATTTGATAGGCATAACGCTTCCCGAGACAGTTAGCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAAt                                             <  2:1896155/140‑1 (MQ=255)

                                                                                           gcgcTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCCTCAATCGCTGTCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACTCCTGATCAGATTGCCAATAg                                           <  2:1967105/140‑1 (MQ=255)

                                                                                           gcgcTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAg                                           <  2:300478/140‑1 (MQ=255)

                                                                                           gcgcTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAg                                           <  2:3168076/140‑1 (MQ=255)

                                                                                            cgcTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGt                                          <  1:789429/140‑1 (MQ=255)

                                                                                             gcTGGATTGTGCATTGTGGGATGTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTg                                         >  2:3528757/1‑140 (MQ=255)

                                                                                             gcTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTg                                         <  1:3235536/140‑1 (MQ=255)

                                                                                                ggATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCAt                                      <  1:703076/140‑1 (MQ=255)

                                                                                                ggATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCAt                                      >  1:1850185/1‑140 (MQ=255)

                                                                                                 gATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                                      >  2:1258302/1‑123 (MQ=255)

                                                                                                 gATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                                      <  1:1258302/123‑1 (MQ=255)

                                                                                                 gATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATc                                     >  2:395792/1‑140 (MQ=255)

                                                                                                   ttGTGCATTGTGGCATCTGGCAGCGCGAAGCCCGCAGCACTCACTGGCTGATTTCCTCGGTATAACGCTTCCCGAGACAGTTATCACTGCACAGATGGTTGTCATCGGTACGCCTGATCACATGGCCAATAGTGCATCaa                                   <  2:2744335/140‑1 (MQ=255)

                                                                                                     gtgCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAaca                                 <  1:919625/140‑1 (MQ=255)

                                                                                                      tgCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAacac                                >  1:535227/1‑140 (MQ=255)

                                                                                                      tgCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAacac                                >  2:4279203/1‑140 (MQ=255)

                                                                                                      tgCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAacac                                >  1:2534999/1‑140 (MQ=255)

                                                                                                      tgCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAacac                                >  2:3230844/1‑140 (MQ=255)

                                                                                                      tgCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAacac                                >  1:4950021/1‑140 (MQ=255)

                                                                                                      tgCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAacac                                >  2:4563497/1‑140 (MQ=255)

                                                                                                      tgCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAacac                                >  1:4344742/1‑140 (MQ=255)

                                                                                                      tgCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCAGTACGGCTGATCAGATGGCCAATAGTGCATCAacac                                >  2:2332139/1‑140 (MQ=255)

                                                                                                        cATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACActc                              >  2:1666987/1‑140 (MQ=255)

                                                                                                        cATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACActc                              <  1:4073598/140‑1 (MQ=255)

                                                                                                           tgtgGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGt                                                                                              <  1:3131138/73‑1 (MQ=255)

                                                                                                           tgtgGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTgg                           >  1:1971548/1‑140 (MQ=255)

                                                                                                            gtgGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCAGAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTggc                          >  2:250302/1‑140 (MQ=255)

                                                                                                             tgGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTggca                         >  1:4819783/1‑140 (MQ=255)

                                                                                                                gATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTggcaggc                      >  1:3968100/1‑140 (MQ=255)

                                                                                                                gATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGGGCATCAACACTCTggcaggc                      >  2:179786/1‑140 (MQ=255)

                                                                                                                 aTCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTAAGCCTGATCAGATCGCCAATAGTGCATCAACACTCTggcaggca                     <  2:4675121/140‑1 (MQ=255)

                                                                                                                  tCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGAGTAGATGGCAAATAGTGCATCAACACTCTGacaggcag                    >  2:4092574/1‑140 (MQ=255)

                                                                                                                  tCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTGGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGgcaggcag                    >  2:14129/1‑140 (MQ=255)

                                                                                                                     ggCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCTTCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGgcg                 <  2:4482439/140‑1 (MQ=255)

                                                                                                                      gCCGCGCGAAGACAGCAGCAATCGCTGGCTGAATTGATCGGCATAACGCTTACCGACACAGTTATCACTGCACAGACGGTAGTCATAGGTACGGCCGATCACACGGCCAATAGTGTATCAACACTCTGACAGCCAGgcgc                >  2:4844485/1‑140 (MQ=255)

                                                                                                                       ccGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGcgcg               >  1:1737166/1‑140 (MQ=255)

                                                                                                                       ccGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGcgcg               >  1:2373377/1‑140 (MQ=255)

                                                                                                                          cgcgAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGCGCGaaa            >  1:3174186/1‑140 (MQ=255)

                                                                                                                          cgcgAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCAAATAGTGCATCAACACTCTGGCAGGCAGGCGCGaaa            >  2:2158990/1‑140 (MQ=255)

                                                                                                                               aGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGCGCGAAATTACt       <  2:2378365/140‑1 (MQ=255)

                                                                                                                                    gcagcaATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGCGCGAAATTACTGAAAg  <  1:3366219/140‑1 (MQ=255)

GGCGCAAATTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGCGCGAAATTACTGAAAG  >  NZ_CP025268/1393924‑1394195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`