Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 159,854 G→A F20F (TTC→TTT)  gluQRS ← tRNA glutamyl‑Q(34) synthetase GluQRS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268159,8540GA99.5% 1372.4 / NA 361F20F (TTC→TTT) gluQRStRNA glutamyl‑Q(34) synthetase GluQRS
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (157/202);  total (157/204)
Fisher's exact test for biased strand distribution p-value = 5.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 362 total aligned reads displayed.

GTTCCAGCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGCTGTAATTAGCC  >  NZ_CP025268/159718‑159985
                                                                                                                                        |                                                                                                                                   
    cAGCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGaa                                                                                                                              >  2:3196136/1‑140 (MQ=255)
    cAGCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGaa                                                                                                                              >  2:2161934/1‑140 (MQ=255)
      gCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGc                                                                                                                            <  2:308455/140‑1 (MQ=255)
      gCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGc                                                                                                                            <  2:2975050/140‑1 (MQ=255)
      gCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGc                                                                                                                            >  1:1667152/1‑140 (MQ=255)
         ggCGCAGGATAGTTTCTGCGGCACCGGGAAGTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCg                                                                                                                         >  1:3060006/1‑140 (MQ=255)
          gcgcAGGATAGTTTCTGCGGCACCGGGAGCTTCACGAGGCGGGTCGATATCTTCTATGCGTACCGGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCGGCCGAGCGCGGCGGTCAGAGAGCCGAAATGAAGCTCGc                                                                                                                        <  2:3675042/140‑1 (MQ=255)
          gcgcAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGc                                                                                                                        <  2:413311/140‑1 (MQ=255)
               ggATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGaa                                                                                                                   <  1:4383485/140‑1 (MQ=255)
                          gCGGCCCCGGGAACTTCACGAGGCGGGTCTATATCTGATATGCGTACCAGCGAGAGACCGGGAGGGGCGCGAGCCTGCAAATAGCTGCAGAGAGCGGCGATCAGAGAGTCAAAATAAAGGTCGCCGGAAGGAGAgggggg                                                                                                        >  2:1068618/1‑139 (MQ=255)
                          gCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGc                                                                                                        >  1:430432/1‑140 (MQ=255)
                             gCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGaa                                                                                                     <  1:3411098/140‑1 (MQ=255)
                             gCACCGGGAACTTCACGAGGCGGGTCGATAGCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGaa                                                                                                     >  1:613182/1‑140 (MQ=255)
                               acagggAACTGCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                   <  2:945911/137‑1 (MQ=255)
                               aCCGGGTACTTCACGAGGAGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                   <  2:2462737/140‑1 (MQ=255)
                               aCCGGGCACTTAACGAGGCGGGTCGATATCTTCTAGGCGTCCCAGCCCGCGCCCTTGCCGGGCGCGAGCCTGCAAATCGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                   <  2:2406243/140‑1 (MQ=255)
                               aCCGGGAACTTCCCGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                   <  1:2176995/140‑1 (MQ=255)
                               aCCGGGAACTTCCCGAGGCGGGTCCATATCTTCTATGCGTACCCGCCCGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                   <  2:2015986/140‑1 (MQ=255)
                               aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                   <  2:2860331/140‑1 (MQ=255)
                               aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                   >  1:183042/1‑140 (MQ=255)
                               aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                   <  1:4104771/140‑1 (MQ=255)
                               aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                   <  2:1938181/140‑1 (MQ=255)
                               aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                   <  1:3713578/140‑1 (MQ=255)
                               aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                   <  1:2106972/140‑1 (MQ=255)
                               aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                   <  1:1293230/140‑1 (MQ=255)
                               aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                   <  1:2917210/140‑1 (MQ=255)
                               aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                   <  1:2694422/140‑1 (MQ=255)
                               aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                   <  2:4842028/140‑1 (MQ=255)
                               aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                   <  2:760955/140‑1 (MQ=255)
                               aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                   <  1:2256327/140‑1 (MQ=255)
                                 cGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGTGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCgg                                                                                                 >  1:3646081/1‑140 (MQ=255)
                                 cGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCgg                                                                                                 <  1:1005894/140‑1 (MQ=255)
                                 cGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCgg                                                                                                 <  1:1021939/140‑1 (MQ=255)
                                 cGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCgg                                                                                                 <  2:4550743/140‑1 (MQ=255)
                                 cGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCgg                                                                                                 <  2:2331148/140‑1 (MQ=255)
                                 cGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCgg                                                                                                 <  2:719453/140‑1 (MQ=255)
                                 cGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCgg                                                                                                 <  1:5071638/140‑1 (MQ=255)
                                 cGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCgg                                                                                                 <  1:3959894/140‑1 (MQ=255)
                                  gggAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGc                                                                                                <  1:4972024/140‑1 (MQ=255)
                                   ggAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGcc                                                                                               <  1:2862387/140‑1 (MQ=255)
                                   ggAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGcc                                                                                               <  2:3530622/140‑1 (MQ=255)
                                   ggAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGcc                                                                                               <  1:580276/140‑1 (MQ=255)
                                    gAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCa                                                                                              <  2:2578841/140‑1 (MQ=255)
                                        ttCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCaagag                                                                                          >  1:3539791/1‑137 (MQ=255)
                                         tCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAAtata                                                                                         <  2:1691986/140‑1 (MQ=255)
                                         tCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAAtata                                                                                         <  1:3008923/140‑1 (MQ=255)
                                              aGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGCCCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCAAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACtgtg                                                                                    <  2:505478/140‑1 (MQ=255)
                                                gCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGACGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACtgtgtg                                                                                  >  1:4636655/1‑140 (MQ=255)
                                                 cGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTgtgtgt                                                                                 <  2:1129063/140‑1 (MQ=255)
                                                 cGGGTCGATACCTTCTATGCATCCCAGCCAGCAACCTTACCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCTATCAGAGAGCCAACATGAAGATCGCCGGAAGGATAGCGGGCCAAGCGGCAAATATACTgtgtgt                                                                                 <  2:1564915/140‑1 (MQ=255)
                                                  gggTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGtgtc                                                                                >  1:914753/1‑140 (MQ=255)
                                                    gTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCTGAGAGCCAAAATGAAGCTCGCCAGAAGGAGAGGGAGCGAAGCGGCCGATATACTGCGtgtctg                                                                              >  2:4232358/1‑140 (MQ=255)
                                                    gTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGtgtctg                                                                              >  1:1490664/1‑140 (MQ=255)
                                                    gTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGACAAGATACTGtgtgtgtg                                                                              >  1:1129235/1‑140 (MQ=255)
                                                       gATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCa                                                                           <  2:4461840/140‑1 (MQ=255)
                                                         tatCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCAtc                                                                         >  2:1294471/1‑140 (MQ=255)
                                                              tctATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCTGCCAATATACTGTGTGTCTGTCATCTCttt                                                                    >  2:1422682/1‑140 (MQ=255)
                                                               ctATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTg                                                                   >  2:2059632/1‑140 (MQ=255)
                                                                  tGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAAc                                                                >  2:4753388/1‑140 (MQ=255)
                                                                  tGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCGTTGAAc                                                                >  1:1975659/1‑140 (MQ=255)
                                                                   gCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACa                                                               <  1:3483318/140‑1 (MQ=255)
                                                                   gCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACa                                                               <  2:2217665/140‑1 (MQ=255)
                                                                       aCCAGCCAGCGACCTTGCCGGGCGCGAGTCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCTAATAGACTGTGCGTCTGTCATCTCTTTGACCaaaaa                                                           >  2:539321/1‑140 (MQ=255)
                                                                       aCCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCGAATATACTGTGTGTCTGTCATCTCTTTGAACaaaaa                                                           >  2:3397250/1‑140 (MQ=255)
                                                                       aCCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACaaaaa                                                           >  1:5099260/1‑140 (MQ=255)
                                                                         cagccagcGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAAt                                                         <  1:2529443/140‑1 (MQ=255)
                                                                         cagccagcGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAAt                                                         <  1:2633907/140‑1 (MQ=255)
                                                                                cGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGACGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCATTGAACAAAAAATAAGGCgg                                                  >  2:3820645/1‑140 (MQ=255)
                                                                                cGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACtgtgt                                                                                   <  1:4761232/107‑1 (MQ=255)
                                                                                cGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCgg                                                  >  1:3918510/1‑140 (MQ=255)
                                                                                   ccTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAg                                               <  2:1503878/140‑1 (MQ=255)
                                                                                     ttGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGAGGCGCTCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCa                                             <  2:4266466/140‑1 (MQ=255)
                                                                                      tGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCAt                                            >  2:1482230/1‑140 (MQ=255)
                                                                                      tGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCAt                                            >  1:1773888/1‑140 (MQ=255)
                                                                                             cgcgAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGc                                     <  2:495008/140‑1 (MQ=255)
                                                                                              gcgAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAGCAAAAAATAAGGCGGGAGCATTTCCCGcc                                    <  2:64233/140‑1 (MQ=255)
                                                                                              gcgAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGcc                                    <  2:2713435/140‑1 (MQ=255)
                                                                                              gcgAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGcc                                    <  1:1445774/140‑1 (MQ=255)
                                                                                              gcgAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGcc                                    <  2:1378542/140‑1 (MQ=255)
                                                                                              gcgAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGGGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGcc                                    >  1:7184/1‑140 (MQ=255)
                                                                                              gcgAGCCTGCAAATAGCTGCCGAGCCCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGcc                                    <  2:334359/140‑1 (MQ=255)
                                                                                              gcgAGCCTGCAAATAGCTGCCAAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGcc                                    <  1:747678/140‑1 (MQ=255)
                                                                                                 aGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCtgt                                 >  1:2199894/1‑140 (MQ=255)
                                                                                                    cTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGt                              <  1:2378723/140‑1 (MQ=255)
                                                                                                     tGCAAATAGCTGCCGATCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGGCGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCCTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTa                             <  1:2837934/140‑1 (MQ=255)
                                                                                                       cAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTaaa                           <  1:3789054/140‑1 (MQ=255)
                                                                                                            aGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCgg                                                                                                 >  1:988581/1‑65 (MQ=255)
                                                                                                            aGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGa                      >  1:323143/1‑140 (MQ=255)
                                                                                                              cTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATg                    >  1:1384035/1‑140 (MQ=255)
                                                                                                              cTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATg                    >  2:179512/1‑140 (MQ=255)
                                                                                                              cTGCCGAGAGCGTCGATCAGCGAGCCAAAATGAATCTCGCCGGAAGGAGAGGTAGCAACGCGGCCACTATACAGTGTGTCTGTCATATCTTTGAACATAAAATAAGACGGGAACATTTCACGCCTGTGGTAAACGTGATg                    >  2:1195808/1‑140 (MQ=255)
                                                                                                                 ccGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGaa                 <  1:4890504/140‑1 (MQ=255)
                                                                                                                 ccGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGaa                 <  2:4067149/140‑1 (MQ=255)
                                                                                                                 ccGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGaa                 >  2:4890504/1‑140 (MQ=255)
                                                                                                                 ccGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGaa                 <  2:225229/140‑1 (MQ=255)
                                                                                                                 ccGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGaa                 <  2:4661055/140‑1 (MQ=255)
                                                                                                                 ccGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGaa                 <  2:4432235/140‑1 (MQ=255)
                                                                                                                     gcgcGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGc             <  1:812845/140‑1 (MQ=255)
                                                                                                                               cAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGCTGTAATTagc   <  1:1568632/140‑1 (MQ=255)
                                                                                                                                agagagCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCTGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGCTGTAAGTatcc  >  2:1867181/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                   
GTTCCAGCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGCTGTAATTAGCC  >  NZ_CP025268/159718‑159985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑