Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 162,758 A→G T275A (ACT→GCT)  hrpB → ATP‑dependent helicase HrpB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268162,7580AG99.7% 1230.5 / NA 370T275A (ACT→GCT) hrpBATP‑dependent helicase HrpB
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (188/180);  total (190/180)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 370 total aligned reads displayed.

AACAACTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCA  >  NZ_CP025268/162624‑162891
                                                                                                                                      |                                                                                                                                     
 acaacTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGaaa                                                                                                                                 <  1:3190949/140‑1 (MQ=255)
 acaacTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGaaa                                                                                                                                 <  1:4368911/140‑1 (MQ=255)
   aacTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAAcc                                                                                                                               <  2:1309180/140‑1 (MQ=255)
    acTGGCTTCGCGCATCGGCAGTGGTGTATTGCTCTGCCCGCTGTCTGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCa                                                                                                                              >  2:4834520/1‑140 (MQ=255)
    acTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCa                                                                                                                              >  2:5088353/1‑140 (MQ=255)
    acTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCa                                                                                                                              >  1:996813/1‑140 (MQ=255)
    acTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCa                                                                                                                              >  2:2851231/1‑140 (MQ=255)
    acTGGCTCCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGCAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGGTGGCGACCAATATTGCTGAAACCa                                                                                                                              >  2:2586312/1‑140 (MQ=255)
     cTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAg                                                                                                                             >  1:2281167/1‑140 (MQ=255)
     cTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAg                                                                                                                             >  1:3023455/1‑140 (MQ=255)
       ggCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGtt                                                                                                                           <  1:2059067/140‑1 (MQ=255)
         ttgcgcgCATCGGCAGTGATGTATAGCCCTGACAGCAGTATGGCGCGTTGTCGCTGAACGATAAGAGAAAAGCGATCCTCACGGGACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTa                                                                                                                         <  2:2784619/137‑1 (MQ=255)
         cTTCGCGCATCGGCAGGGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTa                                                                                                                         >  1:2778866/1‑140 (MQ=255)
            cgcgCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAAcc                                                                                                                      >  1:4539978/1‑140 (MQ=255)
               gcctCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAACCCAGTTTAACCAtt                                                                                                                   <  2:3886887/137‑1 (MQ=255)
                 aTCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGa                                                                                                                 <  1:3092776/140‑1 (MQ=255)
                       aGTGATGTATTGCTCTGGCCGCTGTCTGGCGCGTTGTCGCTGAACGATCAGCAAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTAt                                                                                                           <  2:4628957/140‑1 (MQ=255)
                           aTGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGt                                                                                                       <  2:15404/140‑1 (MQ=255)
                            tGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTc                                                                                                      <  1:4455303/140‑1 (MQ=255)
                            tGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTc                                                                                                      <  1:283701/140‑1 (MQ=255)
                                ttGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACTAGTTTAACCATTGAAGGTATGCGTGtggg                                                                                                  >  1:1098872/1‑139 (MQ=255)
                                   ctctGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCtggtggt                                                                                               >  1:2604961/1‑140 (MQ=255)
                                   ctctGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCtggtggt                                                                                               >  1:135738/1‑140 (MQ=255)
                                   ctctGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCtggtggt                                                                                               >  1:3671929/1‑140 (MQ=255)
                                      tGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAGGGGATGCGCAAAGTGGTGCTGGCGACCAATATGGCTGAAACCAGTTTAACCATTGACGGTATTCGTCTGGTGGGGGa                                                                                            >  2:4038827/1‑140 (MQ=255)
                                      tGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGa                                                                                            >  1:2330/1‑140 (MQ=255)
                                      tGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGa                                                                                            >  1:3302751/1‑140 (MQ=255)
                                      tGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGa                                                                                            >  1:4233335/1‑140 (MQ=255)
                                      tGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGa                                                                                            <  2:343931/140‑1 (MQ=255)
                                        ggcGCTGTATGGTGGGTCGTGGCTGAACGATCAGCGAAAGGAGGGCCTCCCGGGAACGCAAGGGATGCGCAAAGTGTTGCTGGCGACCACTATGGCTGAAACCAGTTTAACCATTGAAGGTATGCGTCTGGTGGTGGAtt                                                                                          <  2:1126240/138‑1 (MQ=255)
                                        cccGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGACACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGCtt                                                                                          >  2:3869004/1‑140 (MQ=255)
                                        cccGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCCGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGAtt                                                                                          >  1:1568434/1‑140 (MQ=255)
                                        cccGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTaa                                                                                                                        >  2:2463325/1‑110 (MQ=255)
                                        cccGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGAtt                                                                                          <  2:412597/140‑1 (MQ=255)
                                        cccGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGAtt                                                                                          <  1:5120835/140‑1 (MQ=255)
                                        cccGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGAtt                                                                                          <  2:395625/140‑1 (MQ=255)
                                        cccGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGAtt                                                                                          >  1:4945375/1‑140 (MQ=255)
                                        cccGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGAtt                                                                                          <  1:4797257/140‑1 (MQ=255)
                                        cccGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGAtt                                                                                          <  2:3115127/140‑1 (MQ=255)
                                        cccGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGAtt                                                                                          <  2:1756803/140‑1 (MQ=255)
                                        cccGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGAtt                                                                                          <  1:4020681/140‑1 (MQ=255)
                                        cccGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGAtt                                                                                          <  1:3764968/140‑1 (MQ=255)
                                        cccGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGAtt                                                                                          <  1:3142323/140‑1 (MQ=255)
                                             tGTATGGCGCGTTGTCGCTGAACGATCTGCGAAAAGTGATCCTCCCGGCACCGCAAGGGCTGCGCAAAGTGGTGCTGGCGACCATTATTGCTGAAAGCAGTTTAACCATTTACGGTATTCGTCTGTTGGTGGATTTTGcg                                                                                     >  2:4417782/1‑139 (MQ=255)
                                             tGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGcc                                                                                     >  2:1889051/1‑140 (MQ=255)
                                             tGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGcc                                                                                     >  1:2512025/1‑140 (MQ=255)
                                                  ggCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCCAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCCTTGAAGGTATTCGTCTGGTGGTGGATTGTTCCCAGGa                                                                                >  2:2724320/1‑140 (MQ=255)
                                                  ggCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATGCGTCTGGTGGTGGATTGTGCCCAGGa                                                                                >  2:1590374/1‑140 (MQ=255)
                                                  ggCGCGTTGTCGCTGAACGAGCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGa                                                                                >  1:1990470/1‑140 (MQ=255)
                                                     gcgTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCg                                                                             >  1:3437665/1‑140 (MQ=255)
                                                      cgTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACTGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACAAGTTTAACCATTGAAGGTATTCGTGTGGTGGTGGATTGTGCCCAGGAGCgt                                                                            <  1:3062904/140‑1 (MQ=255)
                                                       gTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCgtg                                                                           >  1:3986758/1‑140 (MQ=255)
                                                       gTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCgtg                                                                           >  2:3731575/1‑140 (MQ=255)
                                                         tGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTACCCATTGCAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGtgtg                                                                         >  1:2720123/1‑140 (MQ=255)
                                                         tGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGtgtg                                                                         >  1:1582337/1‑140 (MQ=255)
                                                         tGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGACCAGGAGCgtggg                                                                         >  2:3723666/1‑138 (MQ=255)
                                                           tCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGgc                                                                       <  2:1351093/140‑1 (MQ=255)
                                                           tCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGgc                                                                       <  2:4964632/140‑1 (MQ=255)
                                                                  aCGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGtttt                                                                <  1:1264067/140‑1 (MQ=255)
                                                                     tccAGCGCAAAGCGACCCTCCCGGCACCGCCAGGGATGCGCACAGTGGTGCTGGCGACCAATATTGCTGAAACCAGCTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGAt                                                             <  2:657569/138‑1 (MQ=255)
                                                                     aTCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGAt                                                             >  2:3046363/1‑140 (MQ=255)
                                                                     aTCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGAt                                                             <  2:2570185/140‑1 (MQ=255)
                                                                       cAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGgc                                                                       >  2:907332/1‑128 (MQ=255)
                                                                        aGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCcg                                                          >  1:716732/1‑140 (MQ=255)
                                                                            aaaaGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCa                                                      <  2:4278006/140‑1 (MQ=255)
                                                                            aaaaGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCa                                                      <  2:3190955/140‑1 (MQ=255)
                                                                            aaaaGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCa                                                      <  2:4928408/140‑1 (MQ=255)
                                                                            aaaaGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCa                                                      <  2:1826938/140‑1 (MQ=255)
                                                                            aaaaGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCa                                                      <  2:201756/140‑1 (MQ=255)
                                                                            aaaaGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCa                                                      <  1:4358447/140‑1 (MQ=255)
                                                                            aaaaGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCa                                                      <  1:4752070/140‑1 (MQ=255)
                                                                            aaaaGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCa                                                      <  2:2241098/140‑1 (MQ=255)
                                                                              aaGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGATGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACg                                                    >  2:1666314/1‑140 (MQ=255)
                                                                                gCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACggg                                                  <  2:3309958/140‑1 (MQ=255)
                                                                                       tccCGGCAACGCAAGGGATGCGCAAAGTGCTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATCGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAcg                                           <  2:4004661/140‑1 (MQ=255)
                                                                                               ccGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGAt                                   <  1:933266/140‑1 (MQ=255)
                                                                                                  cAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAGGGAATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTGTTGATACGCGCACGGGGCTTAAGCGACTGATTAc                                >  2:957020/1‑140 (MQ=255)
                                                                                                  cAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTAc                                >  1:238313/1‑140 (MQ=255)
                                                                                                  cAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTAc                                >  1:1827240/1‑140 (MQ=255)
                                                                                                  cAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTGTAACCATTGAAGGTATTCGTCTGGTGGAGGGTTGTGCCCAGGAGCGTGTGGCGCGGTTTGATACGCGCACGGGGCTTACGCGACTGATTAc                                >  2:3489931/1‑140 (MQ=255)
                                                                                                  cAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTCCTGAAACCAGTTTGACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCACAGGCGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTAc                                >  2:1446450/1‑140 (MQ=255)
                                                                                                    aGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTc                              <  1:904194/140‑1 (MQ=255)
                                                                                                     gggATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCa                             <  2:1242579/140‑1 (MQ=255)
                                                                                                     gggATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCa                             <  1:4910433/140‑1 (MQ=255)
                                                                                                     gggATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGAGCCGCGCACGGGGCTTACGCGACTGATTACTCa                             <  2:3587496/140‑1 (MQ=255)
                                                                                                          gcgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAcgcg                        <  1:1189471/140‑1 (MQ=255)
                                                                                                          gcgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGAGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAcgcg                        >  1:569057/1‑140 (MQ=255)
                                                                                                          gcgcAAAGTGGTGATGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGAGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAcgcg                        <  2:4679080/140‑1 (MQ=255)
                                                                                                           cgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGt                       >  1:1654756/1‑140 (MQ=255)
                                                                                                           cgcAAAGTGGCGCTGGCGACCAATCTGGCTGAAACCAGTTTAACCATTGAAGGTACCCGTCTGGCGCTGGATTGTGCCCAGGCGCGTGTGGCGCGTTTGGATCCGCGCACGGGGCTGACGCGACTGAGTACCCCCCGCGt                       >  2:1986626/1‑140 (MQ=255)
                                                                                                                aGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGcc                  >  1:194526/1‑140 (MQ=255)
                                                                                                                    gtgCTGTCGACCAATTTTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTAGTGGATTCTCCCCCGGAGCGTGTGGCGCGTTTTTATCCGCGCACGGGGCATACGCGACTGATTCCTCAACGCGTTAGCCAGGc              <  2:2908302/140‑1 (MQ=255)
                                                                                                                    gtgCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGc              <  2:80446/140‑1 (MQ=255)
                                                                                                                     tgCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCa             >  1:1020340/1‑140 (MQ=255)
                                                                                                                     tgCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCa             >  1:3837488/1‑140 (MQ=255)
                                                                                                                      gCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCAt            <  1:3731575/140‑1 (MQ=255)
                                                                                                                      gCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGAGCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCAt            <  2:3536204/140‑1 (MQ=255)
                                                                                                                          gCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCAt        >  1:4851207/1‑140 (MQ=255)
                                                                                                                              ccAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACg    >  1:2951526/1‑140 (MQ=255)
                                                                                                                                aaTATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCa  <  1:1425513/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                     
AACAACTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCA  >  NZ_CP025268/162624‑162891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑