BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,675,399	A→G	R295G (AGA→GGA)	ynfM →	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,675,399	0	A	G	99.7%	1096.4 / NA	330	R295G (AGA→GGA)	ynfM	MFS transporter
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (158/169); total (159/171)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 330 total aligned reads displayed.

GCTGATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCTCAGCA  >  NZ_CP025268/1675263‑1675530

 cTGATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAAc                                                                                                                                 <  2:3615266/140‑1 (MQ=255)

   gATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCa                                                                                                                               <  2:4659370/140‑1 (MQ=255)

       gggTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGac                                                                                                                           <  1:4533081/140‑1 (MQ=255)

         gTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGacca                                                                                                                         >  2:1872468/1‑140 (MQ=255)

          tcgttcgtCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGaccac                                                                                                                        <  1:947627/140‑1 (MQ=255)

           cgttcgtCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTTGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCCAAGCCGGAACCATGaccacc                                                                                                                       <  2:5052080/140‑1 (MQ=255)

           cgttcgtCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGaccacc                                                                                                                       <  1:3196516/140‑1 (MQ=255)

           cgttcgtCACGATGTTTAATTACAACGGCTCTCGGTTGTAGCTTTCCCCCTGGCATGTCAGTCAGGACGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGaccacc                                                                                                                       <  2:4170845/140‑1 (MQ=255)

                gtCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTa                                                                                                                  <  2:2139634/140‑1 (MQ=255)

                gtCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTa                                                                                                                  <  2:4349515/140‑1 (MQ=255)

                      ttGTTTAATTACATCGGCTATGGGTTGATGCTCTCCCCCTGGTATGTCAGTCAGTCCGTGGTTGGCTTATTATCGATGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGACGGAAGCATGACCACCCGCTATGGGCg                                                                                                            <  2:4130581/139‑1 (MQ=255)

                      cTGTTTAATTACATCGGCTATCGGTTGATGCTTTCCCCCGGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCg                                                                                                            <  2:1916198/140‑1 (MQ=255)

                      cTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCg                                                                                                            <  1:2937849/140‑1 (MQ=255)

                      cTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCg                                                                                                            <  2:3087509/140‑1 (MQ=255)

                      cTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCg                                                                                                            <  1:4003857/140‑1 (MQ=255)

                      cTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCg                                                                                                            <  1:2535998/140‑1 (MQ=255)

                      cTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCg                                                                                                            <  2:434152/140‑1 (MQ=255)

                      cTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCg                                                                                                            <  1:1355706/140‑1 (MQ=255)

                      cTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCg                                                                                                            >  2:569894/1‑140 (MQ=255)

                      cTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCGTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCg                                                                                                            <  1:1103986/140‑1 (MQ=255)

                      cTGTTTAATTACATCGGCTATCAGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTAGTTGACCGGAACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCg                                                                                                            <  2:4011702/140‑1 (MQ=255)

                      cTGTTTAATACCATCGGCTATCGGTTGATCCTCTCCCCCTGGCATGTCAGTCAGGCAGTGGTTGACTAATTATAGCTGGCTTATCTGCCCCCTACATGGAGCTAACACAAAGCCGGAACCATGACCACCCGCTATGGGCg                                                                                                            <  2:4066293/140‑1 (MQ=255)

                          ttAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGt                                                                                                        >  1:4858930/1‑140 (MQ=255)

                          ttAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGt                                                                                                        >  1:2524006/1‑140 (MQ=255)

                           tAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTc                                                                                                       >  1:1619303/1‑140 (MQ=255)

                               tACATCGGCTATCGGGTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGGCCAGt                                                                                                   >  2:3430340/1‑140 (MQ=255)

                                 cATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGa                                                                                                 >  1:2133500/1‑140 (MQ=255)

                                   tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAtg                                                                                               >  1:3538930/1‑140 (MQ=255)

                                   tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAtg                                                                                               >  1:193528/1‑140 (MQ=255)

                                   tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAtg                                                                                               >  2:4518430/1‑140 (MQ=255)

                                   tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGGGGGTGGCTTATTATCGCTGGCTTGTTTGACGGGTACATGGAGCGCACCCAAAGCCGGAACCATGACCGCCCGCGATTGGCGTGGTCCCGTGAtg                                                                                               >  1:3619600/1‑140 (MQ=255)

                                   tCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGAGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAtg                                                                                               >  2:3228215/1‑140 (MQ=255)

                                      gCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAtgttg                                                                                            >  1:3098133/1‑140 (MQ=255)

                                              ttGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGCCCGGTACATGGAGCTCACCCAAAGCGGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAc                                                                                    <  1:4151438/140‑1 (MQ=255)

                                              ttGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCCTGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGAc                                                                                    <  1:3991264/140‑1 (MQ=255)

                                                gATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATGATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCAGGACCACCCGCTATGGGCGTGGTCCAGGGATGTTGTTTTCGACgg                                                                                  >  2:2847798/1‑140 (MQ=255)

                                                 aTGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACggg                                                                                 >  1:2717081/1‑140 (MQ=255)

                                                   gCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACggggg                                                                               <  2:4638946/140‑1 (MQ=255)

                                                    ctctCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTCTCGCTGGCTTCTTTGACCGGTACATGGAGCTCACCCACAGCCGGAACCATGACCCCCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGt                                                                              >  1:467991/1‑140 (MQ=255)

                                                        cccccTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATg                                                                          >  2:941008/1‑140 (MQ=255)

                                                        cccccTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCAGGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATg                                                                          >  2:2262524/1‑140 (MQ=255)

                                                        cccccTGGCATGTCAGACAGGCCGTGGTTGGCTTAGTATCGCTGGCGTATTAGACCGGTACATGGAGCTCACCCATAGCCGGAACCATGACCACCCGCTATGGGCGAGGTCCAGTGATGTTGTTTTCGACGGGGGTTAta                                                                          >  2:460585/1‑139 (MQ=255)

                                                            cTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGt                                                                      >  2:3946336/1‑140 (MQ=255)

                                                            cTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGt                                                                      >  2:2561332/1‑140 (MQ=255)

                                                               gCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTg                                                                   <  2:1627586/140‑1 (MQ=255)

                                                               gCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTg                                                                   >  2:865413/1‑140 (MQ=255)

                                                                 aTGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGt                                                                 <  1:5023722/140‑1 (MQ=255)

                                                                   gtcagtcaGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGttt                                                               >  2:797902/1‑140 (MQ=255)

                                                                   gtcagtcaGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGttt                                                               >  2:3375478/1‑140 (MQ=255)

                                                                   gtcagtcaGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGttt                                                               >  2:1013825/1‑140 (MQ=255)

                                                                   gtcagtcaGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGttt                                                               >  1:642989/1‑140 (MQ=255)

                                                                   gtcagtcaGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACACGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGttt                                                               >  2:3178631/1‑140 (MQ=255)

                                                                    tcagtcagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGt                                                                 <  2:4833606/137‑1 (MQ=255)

                                                                    tcagtcagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGt                                                                 >  1:4832445/1‑137 (MQ=255)

                                                                        tcagGCCGTGGTTTGCTTATTATCGCTGGCTTATTTGACCGGTACTTGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATCGGCGTGGTCCAGTGATGCTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                          <  2:4422126/140‑1 (MQ=255)

                                                                        tcagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                          <  1:4426163/140‑1 (MQ=255)

                                                                        tcagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                          <  2:3107179/140‑1 (MQ=255)

                                                                        tcagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                          <  2:3667114/140‑1 (MQ=255)

                                                                        tcagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                          <  2:1585129/140‑1 (MQ=255)

                                                                        tcagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtga                                                          <  1:1000926/140‑1 (MQ=255)

                                                                        gccagCCGTGGTTGTCTTATTATCGCTGGCCTATTTAACCGGTGCATGGAGCTCACCCAAAGCCGGAGTCTTGATCACCCGCTACTGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTGTGGTGTTTGGTTTTCtga                                                          <  2:3309449/136‑1 (MQ=255)

                                                                         cagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCCAAGCCGGAACCATGACCCCCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtgat                                                         >  1:1685382/1‑140 (MQ=255)

                                                                         cagGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtgat                                                         >  1:856641/1‑140 (MQ=255)

                                                                          agGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACtgatg                                                        >  2:636810/1‑140 (MQ=255)

                                                                             ccGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGAcc                                                     <  1:517967/140‑1 (MQ=255)

                                                                                  gTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCTAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTAtt                                                >  2:3562/1‑140 (MQ=255)

                                                                                     ggCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTCCTGATGACCTTATTCAg                                             <  1:420936/140‑1 (MQ=255)

                                                                                      gCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGc                                            >  1:1704301/1‑140 (MQ=255)

                                                                                          attatCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGc                                        >  2:3885714/1‑140 (MQ=255)

                                                                                          attatCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGc                                        <  1:3719177/140‑1 (MQ=255)

                                                                                          attatCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGc                                        <  2:2035782/140‑1 (MQ=255)

                                                                                           ttatCGCTTGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCt                                       <  2:4733166/140‑1 (MQ=255)

                                                                                                gCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTGTGGTTTACTGATGACCTTATGCAGCTCGCTGTGGc                                  >  2:1816798/1‑140 (MQ=255)

                                                                                                 cTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCt                                 >  1:1235160/1‑140 (MQ=255)

                                                                                                          ttGACCGCTACATGGAGCTCACCCAAAGCCGGAGCCATGACCACCCCCTATGGGGTTGGTCCAGTGCTGTTGTTGTCGACGGGGGTTATGCTGTTTGGTCTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGc                        <  2:2982661/140‑1 (MQ=255)

                                                                                                            gACCGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCg                      <  1:1168045/140‑1 (MQ=255)

                                                                                                              ccGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGa                    >  1:3085623/1‑140 (MQ=255)

                                                                                                               cGGTACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGaa                   <  1:2774399/140‑1 (MQ=255)

                                                                                                                  tACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGAGCTTATTCAGGTCGCTGTGGCTGATCTTTGCCGGAATGt                >  2:1144403/1‑140 (MQ=255)

                                                                                                                  tACATGGAGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGt                >  2:1212301/1‑140 (MQ=255)

                                                                                                                         aGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACtctt         <  1:3424894/140‑1 (MQ=255)

                                                                                                                         aGCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACtctt         <  1:4347956/140‑1 (MQ=255)

                                                                                                                          gCTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACtcttc        >  1:887940/1‑140 (MQ=255)

                                                                                                                           cTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACtcttct       >  1:2216127/1‑140 (MQ=255)

                                                                                                                           cTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACtcttct       >  2:4751547/1‑140 (MQ=255)

                                                                                                                           cTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACtcttct       >  1:4580254/1‑140 (MQ=255)

                                                                                                                           cTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACtcttct       >  1:2273656/1‑140 (MQ=255)

                                                                                                                           cTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACtcttct       >  2:4255638/1‑140 (MQ=255)

                                                                                                                           cTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGGTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTGTGCCGGAATGTTACtcttct       >  2:1114625/1‑140 (MQ=255)

                                                                                                                           cTCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGGTGTTTTCGACGGGGGGTATGCTGTGTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACtcgtct       >  2:1045465/1‑140 (MQ=255)

                                                                                                                            tCACCCAAAGCCGGAAGCATGACCACCCGCTATAGGCTTGGTCCAGGGATGTTGTGTTCCACGGGGGTTATGCTGTTTGTTTGACTGATGACTTTATTAAGCTCTCTGTGGCTGGTCTTTGCCGGAATGTTACTATtatc      >  2:136705/1‑140 (MQ=255)

                                                                                                                            tCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTtctc      >  1:2444730/1‑140 (MQ=255)

                                                                                                                            tCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTtctc      >  1:1495454/1‑140 (MQ=255)

                                                                                                                            tCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTtctc      >  1:4922736/1‑140 (MQ=255)

                                                                                                                            tCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTtctc      >  2:2692294/1‑140 (MQ=255)

                                                                                                                            tCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGATT‑CTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCTca     >  1:2852097/1‑140 (MQ=255)

                                                                                                                            tCACCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGAGGTTGGTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGTCCTTATTCAGCTCGCTGTGGATGATCTTTGCCGGAAGGTTACGCTtctc      >  2:2401960/1‑140 (MQ=255)

                                                                                                                              aaccAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGCCCAGTGATGTTGTTGTCGACGGGGGTTATGCTGTTTGGTTTACTCATGACCTTATTCAGCTCGCTGTGGCTGAACTTTGCCGGAATGTTACTCTTCTcag    <  2:263859/138‑1 (MQ=255)

                                                                                                                              aCCCAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCTcag    >  1:2083252/1‑140 (MQ=255)

                                                                                                                                ccAAAGCCGGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCTcagca  <  2:2532795/140‑1 (MQ=255)

GCTGATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCTTATTATCGCTGGCTTATTTGACCGGTACATGGAGCTCACCCAAAGCCAGAACCATGACCACCCGCTATGGGCGTGGTCCAGTGATGTTGTTTTCGACGGGGGTTATGCTGTTTGGTTTACTGATGACCTTATTCAGCTCGCTGTGGCTGATCTTTGCCGGAATGTTACTCTTCTCAGCA  >  NZ_CP025268/1675263‑1675530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`