BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,696,010	A→G	Q447Q (CAA→CAG)	ydgA →	YdgA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,696,010	0	A	G	99.6%	801.1 / NA	239	Q447Q (CAA→CAG)	ydgA	YdgA family protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (74/163); total (75/164)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 239 total aligned reads displayed.

TGGATGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTA  >  NZ_CP025268/1695874‑1696143

 ggATGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGGTCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGt                                                                                                                                   >  1:4075334/1‑140 (MQ=255)

 ggATGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGGATCAGCAATGGGGCAGATGt                                                                                                                                   >  2:4301318/1‑140 (MQ=255)

 ggATGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGt                                                                                                                                   <  1:4982276/140‑1 (MQ=255)

   aTGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTc                                                                                                                                 <  1:4421417/140‑1 (MQ=255)

   aTGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTc                                                                                                                                 <  2:1195816/140‑1 (MQ=255)

   aTGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTc                                                                                                                                 <  1:1781895/140‑1 (MQ=255)

        aaaCTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCt                                                                                                                            <  1:251867/140‑1 (MQ=255)

          aCTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAACAACTGGCGAAACAGCAACTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGa                                                                                                                          <  2:494678/140‑1 (MQ=255)

           cTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGac                                                                                                                         <  1:111968/140‑1 (MQ=255)

           cTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGac                                                                                                                         <  2:2842012/140‑1 (MQ=255)

             gACCATTCCGGTGGATGTGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGacca                                                                                                                       <  1:2842685/140‑1 (MQ=255)

             gACCATTCCGGTGGATCCGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTCTCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGacca                                                                                                                       <  1:2822807/140‑1 (MQ=255)

             gACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGacca                                                                                                                       <  2:3145736/140‑1 (MQ=255)

             gACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGacca                                                                                                                       <  1:4435145/140‑1 (MQ=255)

             gACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGacca                                                                                                                       <  2:4689181/140‑1 (MQ=255)

             gACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGacca                                                                                                                       <  1:1021557/140‑1 (MQ=255)

             gACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGacca                                                                                                                       <  2:3262178/140‑1 (MQ=255)

             gACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGacca                                                                                                                       <  2:1247264/140‑1 (MQ=255)

             gACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGacca                                                                                                                       <  2:1526219/140‑1 (MQ=255)

             gACCAGACCGTTGGATATGGCAACTGTGTTTATCAGTCAGGGAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGTTGCATCAGCAATGGGGCAGATGTGCCGTCTAacca                                                                                                                       <  2:2198260/140‑1 (MQ=255)

             gACCACTCCGGTGGATATGGCAACTGAGTTTATGCCTCAGGTAGCGAAGCTGGAAGGTTATCTGGAAGATCAAGCGAAAAAACTGGCGAAACAACAAGTCGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGacca                                                                                                                       <  2:4507231/140‑1 (MQ=255)

                  ttCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTg                                                                                                                  <  1:563574/140‑1 (MQ=255)

                  ttCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTg                                                                                                                  <  2:1361267/140‑1 (MQ=255)

                  ttCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTg                                                                                                                  <  1:5078669/140‑1 (MQ=255)

                  ttCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTg                                                                                                                  <  2:545441/140‑1 (MQ=255)

                  ttCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTg                                                                                                                  <  1:776245/140‑1 (MQ=255)

                  ttCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTg                                                                                                                  <  1:22521/140‑1 (MQ=255)

                  ttCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTg                                                                                                                  <  1:1420864/140‑1 (MQ=255)

                  ttCCGGTGGATATGGCAACTGAGGTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTg                                                                                                                  <  2:543592/140‑1 (MQ=255)

                  ttCCGGTGGATATGGCAACTCAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTg                                                                                                                  <  2:1642917/140‑1 (MQ=255)

                  gtCCGGTGGCTATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTg                                                                                                                  <  2:1954760/139‑1 (MQ=255)

                   tCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGc                                                                                                                 >  1:1713680/1‑140 (MQ=255)

                   tCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGc                                                                                                                 >  2:1875338/1‑140 (MQ=255)

                   tCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGc                                                                                                                 >  1:3006722/1‑140 (MQ=255)

                     cGGTGGATATGGCAACTGATTTTATGACTCAGGTATCGAAGCTGTAAGGTGATCAGGAAGAGCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGCGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAg                                                                                                               <  2:2752120/140‑1 (MQ=255)

                     cGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAg                                                                                                               <  2:3040008/140‑1 (MQ=255)

                       gTGGATATTGCAACTGAGTTTATTACTCAGGTAGCGAAGCTTGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGa                                                                                                             <  2:4869734/140‑1 (MQ=255)

                       gTGAATATTGAAACTGACTGTATGACCAAGATAGCGAAGGTGGAAGGTTAGAAGGAAGATCAACCGACAAAACTGGCGAACCAGCAAGTTCAAGGTGCACCAGCACTGGGGCAAATGTTCCGTCTGACCACCTTGCAGGa                                                                                                             <  2:3159897/140‑1 (MQ=255)

                           atatGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAAt                                                                                                         <  2:3822249/140‑1 (MQ=255)

                           atatGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAAt                                                                                                         <  1:2185830/140‑1 (MQ=255)

                            tatGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATa                                                                                                        >  1:2884659/1‑140 (MQ=255)

                             atGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATAc                                                                                                       >  2:2396388/1‑140 (MQ=255)

                               ggCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATAAAGCGAAAAAACTGGCGAAACAGCAAGATGAAGGTGCATCAGCAATGAGGCAGATGTTCCGTCTGACCACCTTGCAGGCCAATAcaa                                                                                                     >  2:1289175/1‑138 (MQ=255)

                                    cTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATcacc                                                                                                >  2:2410676/1‑140 (MQ=255)

                                    cTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTAACAGTAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATcacc                                                                                                >  1:2096391/1‑140 (MQ=255)

                                     tGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATcacca                                                                                               <  2:1713680/140‑1 (MQ=255)

                                     tGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATcacca                                                                                               <  1:4032111/140‑1 (MQ=255)

                                     tGAGATTAGGAATAAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATGAAGCGAAAAAACTTGAGAAACAGCATGTTGAAGGCGCCTCAGCAATGGGGAAGATTTTCCGTCTGACCACCTTTCGGGGCAATACCATaacca                                                                                               <  2:616029/140‑1 (MQ=255)

                                      gAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATcaccac                                                                                              <  2:2989661/140‑1 (MQ=255)

                                       aGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCaccacc                                                                                             >  1:3573333/1‑140 (MQ=255)

                                         tttATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAg                                                                                           <  2:3459293/140‑1 (MQ=255)

                                          ttATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAACCAGAAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGc                                                                                          <  1:3239932/140‑1 (MQ=255)

                                          ttATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATCCCATCACCACCAGc                                                                                          <  1:1174923/140‑1 (MQ=255)

                                          ttATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGc                                                                                          <  1:1242926/140‑1 (MQ=255)

                                           tATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGcc                                                                                         >  2:2258208/1‑140 (MQ=255)

                                           tATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGcc                                                                                         <  1:92740/140‑1 (MQ=255)

                                             tGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTg                                                                                       <  2:4610248/140‑1 (MQ=255)

                                               aCTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCa                                                                                     <  1:1992101/140‑1 (MQ=255)

                                                 tCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAat                                                                                   >  1:1587735/1‑140 (MQ=255)

                                                  cAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAata                                                                                  <  1:4072582/140‑1 (MQ=255)

                                                   aGGTAGCGAAGGTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGCGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAatat                                                                                 >  1:4235112/1‑140 (MQ=255)

                                                   aGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAatat                                                                                 >  1:716592/1‑140 (MQ=255)

                                                   aGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAatat                                                                                 >  1:3044009/1‑140 (MQ=255)

                                                         cGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAAc                                                                           >  2:951599/1‑140 (MQ=255)

                                                         cGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAAc                                                                           >  1:4519597/1‑140 (MQ=255)

                                                           aaccTGGAAGGTTATCAGGAAGATCAAGCTAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACgg                                                                         <  2:2009488/137‑1 (MQ=255)

                                                           aaGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACgg                                                                         <  1:3966089/140‑1 (MQ=255)

                                                           aaGCTGGAAGGTTATCAGGAAGAGCAAGCGAAAAAACTGGGGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACgg                                                                         <  1:4689217/140‑1 (MQ=255)

                                                            aGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGt                                                                        <  2:4454742/140‑1 (MQ=255)

                                                            aGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGt                                                                        <  1:1509346/140‑1 (MQ=255)

                                                                ggAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGa                                                                    <  1:2361641/140‑1 (MQ=255)

                                                                ggAAGGTTATCAAGAAGAGCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGa                                                                    <  2:4995470/140‑1 (MQ=255)

                                                                 gAAGGTTATCAGGAAGCTCAAGCGAAAGACCTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGAt                                                                   <  2:3548416/140‑1 (MQ=255)

                                                                 gAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGAt                                                                   <  2:1410696/140‑1 (MQ=255)

                                                                        aTCAGGAAGATCACGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAACTGGGCAGAGGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTa                                                            >  2:2209213/1‑140 (MQ=255)

                                                                        aTCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTa                                                            >  2:3534690/1‑140 (MQ=255)

                                                                         tCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTaa                                                           <  1:3767234/140‑1 (MQ=255)

                                                                            ggAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATg                                                        <  1:575312/140‑1 (MQ=255)

                                                                              aaGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATggg                                                      >  1:2667539/1‑140 (MQ=255)

                                                                              aaGATCAAGCGAAAAAACT‑GCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGc                                                     <  1:2746499/140‑1 (MQ=255)

                                                                               aGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGc                                                     <  1:3870353/140‑1 (MQ=255)

                                                                               aGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGc                                                     <  1:17208/140‑1 (MQ=255)

                                                                                gagcAAGCGGAAAAACTGGGGAAACATCAAGTTTAAGATGCAGCAGCAATGGGGCATGTGTTTCGTCTGACCACCTTGCAGGGCAATACTAGCACCACCAGCCTGCAGTATGCTAACGGTTATATAACGTTAAATGGGCa                                                    >  2:2644898/4‑140 (MQ=255)

                                                                                gATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCa                                                    >  1:3021333/1‑140 (MQ=255)

                                                                                     aGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGaaaa                                               >  1:3614355/1‑140 (MQ=255)

                                                                                      gCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGCCAGAAAAt                                              <  2:3614355/140‑1 (MQ=255)

                                                                                            aaaCTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGtt                                        <  1:4753135/140‑1 (MQ=255)

                                                                                               cTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTaga                                     <  1:3826731/140‑1 (MQ=255)

                                                                                                 ggCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTagaag                                   >  2:781784/1‑140 (MQ=255)

                                                                                                   cGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATcaccac                                                                                              >  2:3885349/1‑79 (MQ=255)

                                                                                                                   aGGTGCATCCGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTg                 <  1:652211/140‑1 (MQ=255)

                                                                                                                    ggTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGc                <  2:3264776/140‑1 (MQ=255)

                                                                                                                    ggTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGc                <  1:839810/140‑1 (MQ=255)

                                                                                                                        cATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATg            >  1:4111844/1‑140 (MQ=255)

                                                                                                                         aTCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGc           >  1:1424088/1‑140 (MQ=255)

                                                                                                                         aTCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGc           <  1:3751024/140‑1 (MQ=255)

                                                                                                                         aTCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGc           <  1:3114745/140‑1 (MQ=255)

                                                                                                                         aTCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGc           <  2:1008670/140‑1 (MQ=255)

                                                                                                                          tCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGcc          <  2:4557541/140‑1 (MQ=255)

                                                                                                                                aTGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGctct    >  2:4476811/1‑140 (MQ=255)

TGGATGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTA  >  NZ_CP025268/1695874‑1696143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`