BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	14,302	T→C	V45A (GTG→GCG)	dnaJ →	molecular chaperone DnaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	14,302	0	T	C	99.7%	964.8 / NA	293	V45A (GTG→GCG)	dnaJ	molecular chaperone DnaJ
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (155/136); minor base A (1/0); total (157/136)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 293 total aligned reads displayed.

AAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTC  >  NZ_CP025268/14165‑14438

 aaGATGCCTAAGCAAGATTATTACCAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGaa                                                                                                                                       <  2:2759983/140‑1 (MQ=255)

       gCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTaa                                                                                                                                 <  1:2898855/140‑1 (MQ=255)

        cTAAGCAAGATTATTACGATATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTaaa                                                                                                                                <  2:2529330/140‑1 (MQ=255)

        cTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTaaa                                                                                                                                <  2:488902/140‑1 (MQ=255)

        cTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTaaa                                                                                                                                <  1:456798/140‑1 (MQ=255)

        cTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTaaa                                                                                                                                <  1:386353/140‑1 (MQ=255)

        cTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTaaa                                                                                                                                <  1:1661748/140‑1 (MQ=255)

           aGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAagag                                                                                                                             <  1:741014/140‑1 (MQ=255)

                 attattACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAg                                                                                                                       <  1:1762800/140‑1 (MQ=255)

                 attattACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAg                                                                                                                       >  2:2182660/1‑140 (MQ=255)

                 attattACGAGAGTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCGACAAACGCGTGGCCATGAAATACCACGTGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGTTCAAg                                                                                                                       >  2:176589/1‑140 (MQ=255)

                  ttattaCGATGTGTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAgg                                                                                                                      <  2:2067187/140‑1 (MQ=255)

                   tattaCGAGATTTGAGGCGTTGCCAAAGCAGTGGAAGAGCGCGAAATCAGAACGGCCTACAAGCGCAGGGCCATGAAATGCCACCGGGACCGTAACCAGGGTGACAAAGGGGCCGAGGCGAAATGTAAAGAGCTCAAGGa                                                                                                                     <  2:3100817/140‑1 (MQ=255)

                      taCGAGATTTTAGGCGTTTCCAAAACAGCGGGAGAGCGTGAAATCAGAAAGGCCGACAAACGCCGGGCCATGAAATACCACCCGTACCGTAACCAGGGTGACGACGCGGCCGAGGCGAAATCGCAAGAGATCAAGGAAGc                                                                                                                  >  2:1264705/1‑140 (MQ=255)

                       aCGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCt                                                                                                                 >  2:2105218/1‑140 (MQ=255)

                           gaTTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATg                                                                                                             <  2:3176877/140‑1 (MQ=255)

                           gaTTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATg                                                                                                             <  1:2372051/140‑1 (MQ=255)

                               ttAGGCGTTTCCAAAACAGCGGAAGAGCGTGAGATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGt                                                                                                         >  2:2079839/1‑140 (MQ=255)

                                   gCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGTCGAACTGTAAAGAGATCAAGGCAGCTTATGAAGTTCTg                                                                                                     >  2:3786922/1‑140 (MQ=255)

                                        tCCAAAACAGCGGAAGAGCGTGAAATCAGAACGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGa                                                                                                >  1:815790/1‑140 (MQ=255)

                                        tCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGa                                                                                                >  1:123162/1‑140 (MQ=255)

                                        tCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGa                                                                                                >  1:1807309/1‑140 (MQ=255)

                                        tCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGGGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGa                                                                                                >  1:1536980/1‑140 (MQ=255)

                                         ccAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGAc                                                                                               <  2:2741723/140‑1 (MQ=255)

                                                 gCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaa                                                                                       >  1:3963779/1‑140 (MQ=255)

                                                  cGGAAGAGCGTGAACTCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAATAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaacac                                                                                      >  1:4626632/1‑137 (MQ=255)

                                                  cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaaa                                                                                      >  2:4853997/1‑140 (MQ=255)

                                                  cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaaa                                                                                      >  1:2978734/1‑140 (MQ=255)

                                                  cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaaa                                                                                      >  1:4278747/1‑140 (MQ=255)

                                                  cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaaa                                                                                      >  2:2989127/1‑140 (MQ=255)

                                                  cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaaa                                                                                      >  1:3459213/1‑140 (MQ=255)

                                                  cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCC‑GACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGAGCGACTCGCAAAAAc                                                                                     >  1:4914093/1‑140 (MQ=255)

                                                    gAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACg                                                                                    >  2:4931214/1‑140 (MQ=255)

                                                      agagCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTg                                                                                  <  2:231710/140‑1 (MQ=255)

                                                      agagCGTGAAAGCAGAAAGGCATACAAACGCATGGCCATGAAATACCCCCCGGACCGCAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGAGCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTg                                                                                  <  1:1099003/140‑1 (MQ=255)

                                                       gagCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAACGAGATCAAGGAAGCTTATGAAGTTCTGACCGCCTCGCAAAAACGTGc                                                                                 <  1:2169579/140‑1 (MQ=255)

                                                       gagCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCACGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                                                 <  1:4406124/140‑1 (MQ=255)

                                                       gagCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                                                 <  1:1444906/140‑1 (MQ=255)

                                                       gagCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                                                 <  1:1591357/140‑1 (MQ=255)

                                                          cGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGc                                                                              >  1:1925884/1‑140 (MQ=255)

                                                          cGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGc                                                                              >  2:4782175/1‑140 (MQ=255)

                                                          cGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGc                                                                              >  1:2573488/1‑140 (MQ=255)

                                                            tGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAACTTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCAt                                                                            >  1:622335/1‑140 (MQ=255)

                                                            tGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCAt                                                                            <  1:4549626/140‑1 (MQ=255)

                                                            tGAAATCAGAAAGGCCAACAAACGCCTGGCCATAAAATACCACCCGGACCGTAACCCGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGACATCAAAGAAGCTTATGAACTTCTGACCGACTCGCAAAAACGTGCGGCAt                                                                            <  1:1094722/140‑1 (MQ=255)

                                                            ggAAATCAGGAAGGCCTACAAAGGCCTGGGCATTAAATACCACCCGGACCGTAACGAGGGGGACAGAGTGGCCGAGGCGAAATTGAAAGGGATCAAGGAGGCTTATGAAGTTCTGACCGACGCGCAAAAACGTGCGGCAt                                                                            <  1:2521913/139‑1 (MQ=255)

                                                              aaaTCTGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATAc                                                                          <  2:4971872/140‑1 (MQ=255)

                                                               aaTCAGAAAGGCCTACTAACGCATCGCCATGAAATACCACCCGGACCGTAACCGGAGTACCAAAGAGGCCGAGACGAAACTTAATCAGATCAAGCAAGATGATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                         <  2:1968083/140‑1 (MQ=255)

                                                               aaTCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGCTCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                         <  2:749172/140‑1 (MQ=255)

                                                               aaTCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                         <  1:3140133/140‑1 (MQ=255)

                                                               aaTCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                         <  2:661818/140‑1 (MQ=255)

                                                               aaTCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                         <  2:54454/140‑1 (MQ=255)

                                                               aaTCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                         <  1:3410149/140‑1 (MQ=255)

                                                               aaTCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                         <  1:21507/140‑1 (MQ=255)

                                                               aaTCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                         <  2:2397956/140‑1 (MQ=255)

                                                               aaTCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                         <  1:4501608/140‑1 (MQ=255)

                                                                 tCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGAt                                                                       <  2:2573488/140‑1 (MQ=255)

                                                                 tCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGAt                                                                       <  1:4208544/140‑1 (MQ=255)

                                                                    gAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGCGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAg                                                                    <  1:5058563/140‑1 (MQ=255)

                                                                            tACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCa                                                            >  2:4373907/1‑140 (MQ=255)

                                                                            tACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCa                                                            >  2:1377002/1‑140 (MQ=255)

                                                                               aaaCGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCAtgc                                                         <  1:3667593/140‑1 (MQ=255)

                                                                                   gCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCg                                                     >  1:1378999/1‑140 (MQ=255)

                                                                                   gCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCg                                                     >  1:4740864/1‑140 (MQ=255)

                                                                                   gCCTGGCCATGAAATACCACCCGGACCGCAACCAGGGTGACAAAGAGGCCGAGGCGACATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCg                                                     >  2:1106206/1‑140 (MQ=255)

                                                                                    ccTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAATAACGTGCGGCATACGATCAGTATGGTCATGCTGCGt                                                    >  1:5043430/1‑140 (MQ=255)

                                                                                    ccTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGt                                                    >  1:3557806/1‑140 (MQ=255)

                                                                                    ccTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGt                                                    >  2:1691551/1‑140 (MQ=255)

                                                                                        gCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGa                                                <  1:3345504/140‑1 (MQ=255)

                                                                                         ccagtaaaTACCCCCCAGACCGTAACCAGGGTGACAAAGAGGCCGATGCGAAATTTAAAGAGATCAAGGAAGCTGATGAAGTTCTGACCGACTCGCAACAACGTGCGGCATACGATCAGGATGGTCATGCTGCGTTTGAg                                               <  2:4605286/135‑1 (MQ=255)

                                                                                         ccATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAg                                               <  1:3905410/140‑1 (MQ=255)

                                                                                             gAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAg                                           <  2:2958487/140‑1 (MQ=255)

                                                                                             gAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAg                                           >  1:972021/1‑140 (MQ=255)

                                                                                                  accaccCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGc                                      >  2:3697422/1‑140 (MQ=255)

                                                                                                   ccaccCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCa                                     >  2:1574891/1‑140 (MQ=255)

                                                                                                    caccCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCAt                                    >  2:4053498/1‑140 (MQ=255)

                                                                                                       ccGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATggg                                 >  2:2601873/1‑140 (MQ=255)

                                                                                                       ccGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATggg                                 <  1:2251847/140‑1 (MQ=255)

                                                                                                       ccGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGGTTGAGCAAGGTGGCATggg                                 >  2:4186860/1‑140 (MQ=255)

                                                                                                        cGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggc                                >  2:4437459/1‑140 (MQ=255)

                                                                                                          gACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcgg                              >  1:512958/1‑140 (MQ=255)

                                                                                                           aCCGTAACCAGGGTGACAAAGAGGCCGAGGCTAAATTTACAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcggc                             >  1:3225166/1‑140 (MQ=255)

                                                                                                            ccGTAACCAGGGTGCCAAAGAGGCCGAGGCGAAATTGAAAGAGATCAAGGAAGCGTATGAAGTTCTGACCGAGTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcggcg                            <  2:3472399/140‑1 (MQ=255)

                                                                                                               tAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGgcggcggcg                         >  2:1861324/1‑140 (MQ=255)

                                                                                                                     gggTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAg                                               >  1:2092475/1‑112 (MQ=255)

                                                                                                                     gggTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTg                   >  2:1659851/1‑140 (MQ=255)

                                                                                                                      ggTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTgg                  <  1:2755605/140‑1 (MQ=255)

                                                                                                                        tcacatcgagGCCGCCGCGAAATTTAAAGAGATCAAGGTAACTTACGAAGTTCTGACCGGTTAGCATAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggcg                <  2:1390798/133‑1 (MQ=255)

                                                                                                                          aCAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGGAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCAGGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggcggc              >  2:4415623/1‑140 (MQ=255)

                                                                                                                          aCAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggcggc              >  1:3021119/1‑140 (MQ=255)

                                                                                                                          aCAAAGAGCCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGAGGTTTTggcggc              >  2:3605528/1‑140 (MQ=255)

                                                                                                                           cAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTACTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGGTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggcggcg             >  1:1707282/1‑140 (MQ=255)

                                                                                                                              ggagGCCGAGGCGAAATTTAAAGAGCTCAAGTAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCCGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGgcggcggcg          <  2:647121/139‑1 (MQ=255)

                                                                                                                              agagGCCGAGGCGAAATTTAAAGAGCTCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGgcggcggcg          <  2:3390208/140‑1 (MQ=255)

                                                                                                                                agGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGCAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCa        <  2:3351789/140‑1 (MQ=255)

                                                                                                                                agGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCa        <  2:4278747/140‑1 (MQ=255)

                                                                                                                                agGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCa        >  2:1474270/1‑140 (MQ=255)

                                                                                                                                  gCCGAGGCGGAATTTAAAGAGAGCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGTTCATGCTGCGTTTGAGCAAAGTGGCATGGGCGGCGGCTGTGTTGGCGGCGGCGCAgg      >  2:4576029/1‑139 (MQ=255)

                                                                                                                                  gCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGa      >  1:1304992/1‑140 (MQ=255)

                                                                                                                                      aGGCGAAAGTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGGCTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTc  <  2:2493904/140‑1 (MQ=255)

AAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTC  >  NZ_CP025268/14165‑14438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`