Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 1,766,333 A→G C92R (TGT→CGT)  sufD ← Fe‑S cluster assembly protein SufD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252681,766,3330AG99.7% 1168.3 / NA 347C92R (TGT→CGT) sufDFe‑S cluster assembly protein SufD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (152/193);  minor base T (1/0);  total (154/193)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 347 total aligned reads displayed.

CACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTC  >  NZ_CP025268/1766196‑1766468
                                                                                                                                         |                                                                                                                                       
  cGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCa                                                                                                                                     >  1:537801/1‑140 (MQ=255)
      ttGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGCCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGa                                                                                                                                 <  2:3825766/140‑1 (MQ=255)
       tgtgCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGAc                                                                                                                                >  2:3140525/1‑140 (MQ=255)
        gtgCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACa                                                                                                                               <  2:2070946/140‑1 (MQ=255)
               gCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCa                                                                                                                        >  1:509563/1‑140 (MQ=255)
                   tCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCg                                                                                                                    >  1:3426340/1‑140 (MQ=255)
                   tCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCg                                                                                                                    >  1:1367632/1‑140 (MQ=255)
                   tCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCg                                                                                                                    >  1:4536819/1‑140 (MQ=255)
                   tCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCg                                                                                                                    >  1:4902899/1‑140 (MQ=255)
                     cGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCTGGCACGTAACGCCCATCGACAAACACCAGCCGCa                                                                                                                  <  1:3710279/140‑1 (MQ=255)
                     cGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCa                                                                                                                  <  1:4062073/140‑1 (MQ=255)
                      gTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCAc                                                                                                                 <  1:1605346/140‑1 (MQ=255)
                      gTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCAc                                                                                                                 <  2:2884399/140‑1 (MQ=255)
                       tCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTACCGCCCATCGACAAACACCAGCCGCACg                                                                                                                <  2:850401/140‑1 (MQ=255)
                       tCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACg                                                                                                                <  2:2509071/140‑1 (MQ=255)
                       tCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACg                                                                                                                <  2:1506679/140‑1 (MQ=255)
                       tCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACg                                                                                                                <  1:1012327/140‑1 (MQ=255)
                       tCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACg                                                                                                                <  2:4418127/140‑1 (MQ=255)
                       tCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACg                                                                                                                <  1:3016212/140‑1 (MQ=255)
                       tCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACg                                                                                                                <  1:1415155/140‑1 (MQ=255)
                       tCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACg                                                                                                                <  1:4353530/140‑1 (MQ=255)
                       tCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTGCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACg                                                                                                                <  2:4701948/140‑1 (MQ=255)
                       tCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTCCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACg                                                                                                                <  2:4710214/140‑1 (MQ=255)
                         aaaTGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGa                                                                                                              >  1:4799765/1‑140 (MQ=255)
                             gCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCt                                                                                                          <  1:1818097/140‑1 (MQ=255)
                             gCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCt                                                                                                          <  1:4694651/140‑1 (MQ=255)
                                  aaCACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGt                                                                                                     <  1:4869997/140‑1 (MQ=255)
                                     acTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTaa                                                                                                  <  1:2964361/140‑1 (MQ=255)
                                      cTTCCGCGTGAATAGCGTCGGGTAAACCGTGACGGTGGGCGTTAATGCTCACTTCATATCCGCTGCCTGCAGTTGCATCGCTCAGTGCGGGCACGTAACGGCCGTCGACAAACACCAGCCGCACGGAGTCTAAGGTGAAc                                                                                                 >  2:671979/1‑140 (MQ=255)
                                       ttCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACg                                                                                                >  2:1060027/1‑140 (MQ=255)
                                         ccGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCt                                                                                              <  1:475315/140‑1 (MQ=255)
                                          cGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTa                                                                                             <  1:98798/140‑1 (MQ=255)
                                               gtgtAGCTTCGGGCAACCTCTGTTTGTCTTCGTGAATGCTCACTTCATATCCACTCCCTTCAGTTGCATCGCTCAGTGCGCACACGTACAGCCCATCGACAAACACCAGCCGCACGGAGTCTAACCTTCACGCTAAGGCa                                                                                        <  2:1130712/137‑1 (MQ=255)
                                               gAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCa                                                                                        <  2:5005460/140‑1 (MQ=255)
                                                aaTAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGAAAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCAt                                                                                       <  1:155894/140‑1 (MQ=255)
                                                    gCGTCGGGTAAACCGTGACGGTCGTAGTTAATGCTCACTTCCTATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATGACg                                                                                   <  1:1699857/140‑1 (MQ=255)
                                                    gCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg                                                                                   <  1:116508/140‑1 (MQ=255)
                                                    gCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg                                                                                   <  2:1393873/140‑1 (MQ=255)
                                                    gCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg                                                                                   <  2:3497566/140‑1 (MQ=255)
                                                    gCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg                                                                                   <  2:509563/140‑1 (MQ=255)
                                                    gCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg                                                                                   <  1:1195013/140‑1 (MQ=255)
                                                    gCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGCTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg                                                                                   <  2:4611400/140‑1 (MQ=255)
                                                    gCGTCGGGTAAACCCTGACGGTCGTCGTTAATCCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg                                                                                   <  2:565257/140‑1 (MQ=255)
                                                     cGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATGCGCTGCCGTCAGGTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgc                                                                                  >  2:2807982/1‑140 (MQ=255)
                                                       tCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctg                                                                                <  1:2588014/140‑1 (MQ=255)
                                                         gggTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCCTCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctgct                                                                              <  2:2675482/140‑1 (MQ=255)
                                                         gggTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctgct                                                                              <  2:2626478/140‑1 (MQ=255)
                                                          ggTAAACCCTGACGGTCGTCGTTAATGCTGACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGctgctg                                                                             >  1:3231124/1‑140 (MQ=255)
                                                          ggTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGctgctg                                                                             >  2:1465679/1‑140 (MQ=255)
                                                           gTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCtgt                                                                            <  1:4670045/140‑1 (MQ=255)
                                                               aCCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTgggg                                                                        >  1:4310093/1‑140 (MQ=255)
                                                                    gACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTCAGGCATCACGCTGCTGTGGGGATAtc                                                                   >  2:4071017/1‑140 (MQ=255)
                                                                    gACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATAtc                                                                   >  1:2116771/1‑140 (MQ=255)
                                                                     aCGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATAtct                                                                  >  1:2190526/1‑140 (MQ=255)
                                                                         tcgtcgTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTcc                                                              >  2:4570887/1‑140 (MQ=255)
                                                                           gtcgtTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCg                                                            <  1:4947312/140‑1 (MQ=255)
                                                                              gtTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCaa                                                         <  1:2497677/140‑1 (MQ=255)
                                                                              gtTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCaa                                                         <  1:3134078/140‑1 (MQ=255)
                                                                                        aCTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaaa                                               <  1:3949819/140‑1 (MQ=255)
                                                                                        aCTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaaa                                               <  1:2180955/140‑1 (MQ=255)
                                                                                        aCTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaaa                                               <  1:3601781/140‑1 (MQ=255)
                                                                                         cTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAAc                                              >  1:622541/1‑140 (MQ=255)
                                                                                          ttCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACt                                             <  1:3825764/140‑1 (MQ=255)
                                                                                          ttCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACt                                             <  1:2739950/140‑1 (MQ=255)
                                                                                          ttCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACt                                             <  2:1879803/140‑1 (MQ=255)
                                                                                               atCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCtc                                        >  1:2921437/1‑139 (MQ=255)
                                                                                               atCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTa                                        <  2:4799765/140‑1 (MQ=255)
                                                                                                  cGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTg                                     >  2:366142/1‑140 (MQ=255)
                                                                                                   gCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGa                                    <  2:3171793/140‑1 (MQ=255)
                                                                                                    cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACGAACACAAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGAt                                   >  2:2842512/1‑140 (MQ=255)
                                                                                                    cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCAGGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGAt                                   >  2:4403073/1‑140 (MQ=255)
                                                                                                    cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGTAGGCTAACGTTAACGCTAAGGCATCACGCTGCGGTGGTGATATCTCTCCCGCAATGTTGACAAACTGGCTATTGAt                                   >  2:4337409/1‑140 (MQ=255)
                                                                                                    cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAGCGCTAAGGCATCACGCTGCTGTCGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGAt                                   >  2:4853571/1‑140 (MQ=255)
                                                                                                    cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGAt                                   >  2:157297/1‑140 (MQ=255)
                                                                                                    cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGAt                                   >  1:1501529/1‑140 (MQ=255)
                                                                                                    cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGAt                                   >  2:2325223/1‑140 (MQ=255)
                                                                                                     tcccTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGTGGATATCTCTCCCGAAATGCTGACAAACTGGCTATTGATc                                  <  2:1936065/138‑1 (MQ=255)
                                                                                                        cTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGc                               <  1:789926/140‑1 (MQ=255)
                                                                                                                 cATCGCTCAGTGCGGGCACGTAACGCCCATCGCCAAACACCAGCCGCACGGAGTCTAACGGTAACGCTAAGGCATCAAGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGTCCTCCCAgc                      >  2:2314495/1‑140 (MQ=255)
                                                                                                                 cATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCCCGGAGTCTAACTTTAACGCTAAGGCATCACGCTGCTGTGGGGACATCTCACCCGCAATTCTGACAAACTGGCTATTGATCATCCCTTCCAgc                      >  2:2202727/1‑140 (MQ=255)
                                                                                                                   tCGCTCAGTGCGGGCACGTTACGCCCATCGACAAACACCAGCCGCACGGAGACTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCCCTCCCACAATGCGGAGAAACTGGCTATTGATGAGCCCTTCCAacgg                    >  2:832146/1‑140 (MQ=255)
                                                                                                                     gCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAgcggcg                  <  1:4201118/140‑1 (MQ=255)
                                                                                                                      cTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGGTATCTCTCCCGCAATGATGACAAACTGGCTATTGATCCGCCCTTCCAGCGGCGt                 >  2:3692276/1‑140 (MQ=255)
                                                                                                                      cTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGt                 >  1:2427243/1‑140 (MQ=255)
                                                                                                                      cTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGt                 >  1:2282367/1‑140 (MQ=255)
                                                                                                                      cTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGt                 >  1:1203757/1‑140 (MQ=255)
                                                                                                                              gggCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTcc         >  2:3310201/1‑140 (MQ=255)
                                                                                                                                gCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAg       >  2:1374823/1‑140 (MQ=255)
                                                                                                                                   cGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGttt    >  2:2382373/1‑140 (MQ=255)
                                                                                                                                     tAACGCCCCTCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCGAATGCCTCACGCTGCGGTGGGGCTCTCTCTCCCGCAATGCTCACAAACTGGCTATTGATCAGCCCTTCCACCGGCGTATATTTCCAGTTTTc  >  2:492818/1‑140 (MQ=255)
                                                                                                                                     tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTc  >  2:3560235/1‑140 (MQ=255)
                                                                                                                                     tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTc  >  1:2815430/1‑140 (MQ=255)
                                                                                                                                     tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTc  >  2:1282898/1‑140 (MQ=255)
                                                                                                                                     tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTc  >  1:2566894/1‑140 (MQ=255)
                                                                                                                                     tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTc  >  1:4483192/1‑140 (MQ=255)
                                                                                                                                     tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTc  >  2:2906372/1‑140 (MQ=255)
                                                                                                                                     tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTc  >  1:2030745/1‑140 (MQ=255)
                                                                                                                                     tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTc  >  2:737215/1‑140 (MQ=255)
                                                                                                                                     tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTc  >  2:4203925/1‑140 (MQ=255)
                                                                                                                                     tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTc  >  2:2010395/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                       
CACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTC  >  NZ_CP025268/1766196‑1766468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑