Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 1,797,084 T→C *155* (TAA→TAG)  nlpC ← NlpC/P60 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252681,797,0840TC98.9% 301.7 / NA 93*155* (TAA→TAG) nlpCNlpC/P60 family protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (55/36);  total (57/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAA  >  NZ_CP025268/1796948‑1797210
                                                                                                                                        |                                                                                                                              
tttttCAGGGGTCCTTTTTTTAGATTATCCTGATTCTAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAg                                                                                                                             >  2:714869/1‑140 (MQ=255)
tttttCAGGGGTCCTTTTTTTAGATTATCCTGATTATCAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGGCCATTTTTCGACATACTAg                                                                                                                             >  2:4794343/1‑140 (MQ=255)
tttttCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGCTGTATCATCGCCGTCCATTTTTCGACATACTAg                                                                                                                             >  2:714342/1‑140 (MQ=255)
tttttCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAg                                                                                                                             >  2:714426/1‑140 (MQ=255)
       ggggTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGtc                                                                                                                      >  1:4904648/1‑140 (MQ=255)
             cTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCt                                                                                                                >  1:4385608/1‑140 (MQ=255)
                                  tataAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAAt                                                                                           <  1:3665558/140‑1 (MQ=255)
                                    taAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAg                                                                                         <  1:2644233/140‑1 (MQ=255)
                                     aaaCGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGa                                                                                        >  2:1365752/1‑140 (MQ=255)
                                     aaaCGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGa                                                                                        >  2:2655539/1‑140 (MQ=255)
                                     aaaCGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTATCGCCTGGCAGAAATTTTTCTTTCAATaaa                                                                                        >  1:3502270/1‑138 (MQ=255)
                                      aaCGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGATTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTTCCTGCAGTTTTTCTTTTAATAGaa                                                                                       >  1:91870/1‑139 (MQ=255)
                                      aaCGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCTTCAATTTTTCTTTCAATAAAc                                                                                       >  1:91960/1‑140 (MQ=255)
                                      aaCGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGAc                                                                                       <  1:4964460/140‑1 (MQ=255)
                                       aCGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACa                                                                                      <  1:3046956/140‑1 (MQ=255)
                                           ataatCCATTTACGGAATTTTTGGCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATGTTTCTGCCAATAGACATGAt                                                                                  <  1:3742099/140‑1 (MQ=255)
                                            taatCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCGTCTTTCGACATACTAGATTCTTCTCGCCTGCCAGAATTTCTTCTGCCCATATTCATTATc                                                                                 >  1:4247689/1‑140 (MQ=255)
                                               tCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAg                                                                              >  2:346352/1‑140 (MQ=255)
                                                 cATTTACGGAATTTTTGTCTGCCAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTg                                                                            <  1:1740977/140‑1 (MQ=255)
                                                      aCGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGaa                                                                       <  2:820351/140‑1 (MQ=255)
                                                          aaTTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGTCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAAATTTTCTGCCAATAGACCTTATCCAGTTTGGAACgaa                                                                   >  1:1824446/1‑138 (MQ=255)
                                                          aaTTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAAGTTTTCTGCCAATTGACATTATCCAGTGTTGAAAGCa                                                                   >  1:5049865/1‑140 (MQ=255)
                                                           aTTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCAt                                                                  <  1:2859534/140‑1 (MQ=255)
                                                             ttttGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCa                                                                <  1:3130274/140‑1 (MQ=255)
                                                             ttttGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCa                                                                <  1:4764148/140‑1 (MQ=255)
                                                               ttGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACt                                                              <  1:2465338/140‑1 (MQ=255)
                                                               ttGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACt                                                              <  1:2449789/140‑1 (MQ=255)
                                                                  tCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTccc                                                           <  1:168304/140‑1 (MQ=255)
                                                                  tCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTccc                                                           <  1:168443/140‑1 (MQ=255)
                                                                    tGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCtt                                                         <  1:4039762/140‑1 (MQ=255)
                                                                    tGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCtt                                                         <  1:523168/140‑1 (MQ=255)
                                                                     gCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTg                                                        <  1:1503440/140‑1 (MQ=255)
                                                                                  gTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGt                                           <  1:5043231/140‑1 (MQ=255)
                                                                                  gTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGt                                           <  1:1535792/140‑1 (MQ=255)
                                                                                   tATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTATATTCGTCTCGCCTACCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTTCTCGTAGAGGCGTg                                          >  2:1459609/1‑140 (MQ=255)
                                                                                   tATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGATGAACGCATCACTCCCTTGCTGGTAGAGGCGTg                                          >  2:4244931/1‑140 (MQ=255)
                                                                                   tATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTg                                          >  2:371963/1‑140 (MQ=255)
                                                                                   tATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTg                                          >  2:3515268/1‑140 (MQ=255)
                                                                                   tATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTg                                          <  1:2466365/140‑1 (MQ=255)
                                                                                   tATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTg                                          >  2:1349625/1‑140 (MQ=255)
                                                                                   tATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTg                                          <  1:2465676/140‑1 (MQ=255)
                                                                                   tATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCAGTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATGGACATTATCAAGTGATGAACGCATCACTCCCTTTCTGGTAAAGGCGTg                                          >  2:4367252/1‑140 (MQ=255)
                                                                                     ttCAGGAGTAAATGTACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCGGCCAGAAATTTTTTTTTCGTTAGACATTATCCAATAAGGAACGCATTTTTTCCTTGCTGGTTGATGcataaa                                        >  1:2364290/1‑135 (MQ=255)
                                                                                     ttCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGa                                        >  2:1252819/1‑140 (MQ=255)
                                                                                     ttCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGa                                        >  2:4469040/1‑140 (MQ=255)
                                                                                     ttCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGa                                        >  2:676256/1‑140 (MQ=255)
                                                                                     ttCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCGGCCATAAATTTTTCTTCCGATAGACATTATCAAGTGAGGAAAGCATCATTTCCTTGCTGGTATAGGCGTgaa                                        >  1:3699755/1‑138 (MQ=255)
                                                                                     ttCAGGAGTAAATGGACCGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGa                                        >  2:1308101/1‑140 (MQ=255)
                                                                                      tCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAATGCATCACTCCCTTGCTGGTAGAGGCGTGGAt                                       <  1:4564144/140‑1 (MQ=255)
                                                                                        aGGAGTAAATGGACGGCGATGTATCCTCGCTGTCGATTGTTCGACATAATAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGCTAGAGGCGTGGCTaa                                     >  2:2195479/1‑140 (MQ=255)
                                                                                        aGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGCCATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATCGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGTGGCGTGGATaa                                     >  2:2505767/1‑140 (MQ=255)
                                                                                        aGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCACTTGCTGGTAGAGGCGTGGATaa                                     >  2:3414446/1‑140 (MQ=255)
                                                                                        aGGAGTAAATGGACGGCGAAGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAAAGCATCACTCGCTTGCTGGTATAGGCGTGGATaa                                     >  2:3414567/1‑140 (MQ=255)
                                                                                          gAGTAAATGGACGGCGATGTATCATTGCCGTCGATTTTGGGATATACTAGATTCGTCTCGCCTGCCACATATTTTTCTGCCAATGGAGATTATCAAGTGAGGAAGGCATCGCTCCCTGGCTGGTAGAGGCTTGGATCAAt                                   >  2:3228812/1‑140 (MQ=255)
                                                                                          gAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAAt                                   >  2:2848978/1‑140 (MQ=255)
                                                                                          gAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGGAGAGGCGTGGATAAAt                                   >  2:2849010/1‑140 (MQ=255)
                                                                                            gTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTg                                 <  1:3392799/140‑1 (MQ=255)
                                                                                             tAAATGGACGGCGTTGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATTAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTgg                                >  2:325002/1‑140 (MQ=255)
                                                                                             tAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTTCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGTCGTGGATAAATTgg                                >  2:911527/1‑140 (MQ=255)
                                                                                             tAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTgg                                >  2:1764292/1‑140 (MQ=255)
                                                                                              aaaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGgt                               <  1:141496/140‑1 (MQ=255)
                                                                                               aaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATc                                                                                 >  2:4861967/1‑89 (MQ=255)
                                                                                               aaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATc                                                                                 <  1:4861967/89‑1 (MQ=255)
                                                                                               aaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTa                                                  <  1:1845943/120‑1 (MQ=255)
                                                                                               aaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTa                                                  >  2:1845943/1‑120 (MQ=255)
                                                                                               aaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAAtgtgtt                              >  2:289900/1‑135 (MQ=255)
                                                                                               aaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGgtt                              >  2:3426176/1‑140 (MQ=255)
                                                                                               aaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGgtt                              >  2:3427977/1‑140 (MQ=255)
                                                                                                 tGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCTAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGgttgt                            <  1:4243662/140‑1 (MQ=255)
                                                                                                    aCGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTgg                         >  2:824974/1‑140 (MQ=255)
                                                                                                    aCGGCGATGTATCATCGCCGTCCATTTTGCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTTCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGACGCGTGGATAAATTGGttgttgt                         >  2:3103785/1‑139 (MQ=255)
                                                                                                       gCGTTGTATCATCGCCGGCCAGTTTTGGACATATTAGAATCGTCTCGCCTGCCAGCAACTTTTCTGCCAATAGACATTATCAAGATAGGCATGCAACACTCCCTAGCTGGTAGAGGCGTGGTTAAATTCggtgttcgcat                      >  2:2253157/1‑135 (MQ=255)
                                                                                                       gCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCa                                                                >  2:1846911/1‑98 (MQ=255)
                                                                                                       gCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCa                                                                <  1:1846911/98‑1 (MQ=255)
                                                                                                       gCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTAt                      >  2:4882682/1‑140 (MQ=255)
                                                                                                       gCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTAt                      <  1:2395251/140‑1 (MQ=255)
                                                                                                             tATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTCTCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTaaa                <  2:4904648/140‑1 (MQ=255)
                                                                                                             tATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTaaa                <  1:1107867/140‑1 (MQ=255)
                                                                                                             tATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTaaa                <  1:1427899/140‑1 (MQ=255)
                                                                                                             tATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTaaa                <  1:1952437/140‑1 (MQ=255)
                                                                                                             tATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTaaa                <  1:2655539/140‑1 (MQ=255)
                                                                                                                  tcGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCt           >  2:2379811/1‑140 (MQ=255)
                                                                                                                  tcGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCt           >  2:883452/1‑140 (MQ=255)
                                                                                                                  tcGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCt           >  2:1003214/1‑140 (MQ=255)
                                                                                                                     ccGTCCATTTTTCGACATACTCGCTTCGTCTCGCCTGCAAGAAATTTTTCTGCCAATAGACCTTATCACGTGAGGAACGCATCACTCCCTTGATGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTACATCCCTACa        >  2:4975879/1‑140 (MQ=255)
                                                                                                                     ccGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATGACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACa        >  2:3841859/1‑140 (MQ=255)
                                                                                                                     ccGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATCTTTCTGCCACTAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTACATACCTACa        >  2:3985182/1‑140 (MQ=255)
                                                                                                                       gTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATg      >  2:330512/1‑140 (MQ=255)
                                                                                                                       gTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATg      >  2:1114905/1‑140 (MQ=255)
                                                                                                                       gTCCATTTTTCGACATACTAGAGTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATg      >  2:4933616/1‑140 (MQ=255)
                                                                                                                        gccATTTTGCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGCACGCATCACTCACTTGCTGGTAGAGGCGTGAATAAATGGGTTGGTGGTATGGTAAATACCGATATgt     >  2:3200752/2‑139 (MQ=255)
                                                                                                                          aaTTTTTAGACATACTACATTCTTCTCGCCTGCCAGAAATTTTTCTGCCACTAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGCGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACATACATGCaa   <  1:146990/139‑1 (MQ=255)
                                                                                                                           aTTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCaaa  <  1:4860109/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                              
TTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAA  >  NZ_CP025268/1796948‑1797210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑