BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,002,534	T→C	329 (TAA→TAG)	dcyD ←	D‑cysteine desulfhydrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,002,534	0	T	C	99.7%	999.7 / NA	303	329 (TAA→TAG)	dcyD	D‑cysteine desulfhydrase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (156/145); total (157/146)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 303 total aligned reads displayed.

CGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACA  >  NZ_CP025268/2002397‑2002666

cgcgAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTa                                                                                                                                    >  1:1913297/1‑140 (MQ=255)

  cgAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTaaa                                                                                                                                  >  1:984440/1‑140 (MQ=255)

      aaTAAACCCGAGCCGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTg                                                                                                                              <  1:4493290/140‑1 (MQ=255)

          aaCCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGga                                                                                                                          >  2:1316229/1‑140 (MQ=255)

           aCCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGgat                                                                                                                         <  2:2424152/140‑1 (MQ=255)

           aCCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGgat                                                                                                                         <  2:1202765/140‑1 (MQ=255)

           aCCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGgat                                                                                                                         <  1:196995/140‑1 (MQ=255)

                aGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAg                                                                                                                    >  1:3161479/1‑140 (MQ=255)

                     tAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGaa                                                                                                               <  2:1851389/140‑1 (MQ=255)

                     tAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGaa                                                                                                               <  2:2787173/140‑1 (MQ=255)

                      aaGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAAc                                                                                                              <  2:2213433/140‑1 (MQ=255)

                      aaGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAAc                                                                                                              <  1:442236/140‑1 (MQ=255)

                       aGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACa                                                                                                             >  1:584432/1‑140 (MQ=255)

                            aaaaaCATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTTCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCa                                                                                                        >  1:3385479/1‑140 (MQ=255)

                             aaaaCATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAg                                                                                                       >  2:3767553/1‑140 (MQ=255)

                                aCATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGgcg                                                                                                    <  2:2619645/140‑1 (MQ=255)

                                aCATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGgcg                                                                                                    <  2:593622/140‑1 (MQ=255)

                                    gccgccGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCAcc                                                                                                >  1:1734834/1‑140 (MQ=255)

                                      cgccgATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCAccgc                                                                                              <  2:2156203/140‑1 (MQ=255)

                                      cgccgATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCAccgc                                                                                              <  1:1185430/140‑1 (MQ=255)

                                         cgATACTGCGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCgg                                                                                           <  2:2304872/140‑1 (MQ=255)

                                         cgATACTGAGTTGCAGCGTATACCCAGCCCCTTTCCGCAGGAACGGCAAGGAATCAATCCCCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCgg                                                                                           <  2:584939/140‑1 (MQ=255)

                                         cgATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCgg                                                                                           <  1:4305169/140‑1 (MQ=255)

                                         cgATACTGAGTTGCAGCGTATACCCAGCCCCTTGCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCgg                                                                                           <  1:4986448/140‑1 (MQ=255)

                                         cgATACTGAGGTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCgg                                                                                           <  1:2541653/140‑1 (MQ=255)

                                           aTACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTa                                                                                         <  1:2767810/140‑1 (MQ=255)

                                           aTACTGAGTTGCAGCGTATACCCAGCCCCGTTCAGCAGGAACGGCAAGGAATCAATAACCAGATGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGAGAGGCGAACAGCGCAGGCGCACCGCCGGTa                                                                                         <  2:4518357/140‑1 (MQ=255)

                                                 aGTTGCAGCGGATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATa                                                                                   >  2:4430997/1‑140 (MQ=255)

                                                         cGTATACCCAGCCACTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAAt                                                                           <  1:4496554/140‑1 (MQ=255)

                                                           tataCCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCg                                                                         >  1:1554625/1‑140 (MQ=255)

                                                             taCCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGc                                                                       >  2:1977763/1‑140 (MQ=255)

                                                                 cAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGGGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCtt                                                                   <  2:1633048/140‑1 (MQ=255)

                                                                   gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                 >  2:1086389/1‑140 (MQ=255)

                                                                   gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                 >  2:1291963/1‑140 (MQ=255)

                                                                   gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                 >  1:4664431/1‑140 (MQ=255)

                                                                   gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                 >  1:4863076/1‑140 (MQ=255)

                                                                   gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                 >  1:3315930/1‑140 (MQ=255)

                                                                   gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                 >  2:3489401/1‑140 (MQ=255)

                                                                   gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                 <  2:3726157/140‑1 (MQ=255)

                                                                   gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                 >  1:123808/1‑140 (MQ=255)

                                                                   gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                 >  2:4723613/1‑140 (MQ=255)

                                                                   gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                 >  1:1602206/1‑140 (MQ=255)

                                                                    ccccTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCAt                                                                >  1:1010262/1‑140 (MQ=255)

                                                                    ccccTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCAt                                                                >  1:4105784/1‑140 (MQ=255)

                                                                    ccccTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCAt                                                                >  2:97007/1‑140 (MQ=255)

                                                                     cccTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCCTc                                                               >  1:2938437/1‑140 (MQ=255)

                                                                     cccTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATc                                                               >  1:4436974/1‑140 (MQ=255)

                                                                       cgttCAGCAGAAAAGGCAAGGAATCAAGAAGCAGTTGGATACTTTCTTGCAGGAGCGGTTGCGGAGCTAAACGTGGGGATGAGCGGCGAACGGCGCAGGCGCACCGCCGGTAGGAATAAACAGAAGCGGCCCTTCATCtt                                                             <  2:4468031/138‑1 (MQ=255)

                                                                        tttCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCttt                                                            >  1:1401342/1‑140 (MQ=255)

                                                                        tttCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCAAGAGCGTTTCCGGAGCTAAACGTGGGGATGATATGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAAACGGCCCTTCAACttt                                                            >  2:4978497/1‑140 (MQ=255)

                                                                         ttCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTg                                                           >  1:4629452/1‑140 (MQ=255)

                                                                         ttCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTg                                                           >  2:4277990/1‑140 (MQ=255)

                                                                          tCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGa                                                          >  1:1090373/1‑140 (MQ=255)

                                                                           cagcagGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGaa                                                         <  1:670131/140‑1 (MQ=255)

                                                                           cagcagGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGaa                                                         <  1:479367/140‑1 (MQ=255)

                                                                            agcagGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCACGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAg                                                        <  2:4044175/140‑1 (MQ=255)

                                                                              cagGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGTCCCGTCATCTTTGAAGCg                                                      >  2:3620842/1‑140 (MQ=255)

                                                                              cagGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCg                                                      >  1:353662/1‑140 (MQ=255)

                                                                                ggAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGtt                                                    >  1:1264031/1‑140 (MQ=255)

                                                                                 gaccGGCAAGGACTCAATAACCAGTTGTATACTTTCTTGCATGCGCGTTTCCGGAGCTAAACGTGGGGATGATAGGAGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATAGGCCCTTCATCTTTGAAGCGttt                                                   <  2:3242438/137‑1 (MQ=255)

                                                                                 gAACGGCAAGGAATCATTAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGttt                                                   <  1:2843898/140‑1 (MQ=255)

                                                                                   aCGGCAAGGAATCAATAACCAGTTGGTTACTTTCTTGCATGAGCGTTTCCGGGGCGAAACGTGGGGATGAGAGGCGGACAGCGCAGGGGCACTGCCGGTAGGAATAAACAGAATCGGCCCTGCATCTTTGAATCGTGTct                                                 >  2:3878791/1‑140 (MQ=255)

                                                                                         aGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTctgactga                                           <  1:2447507/140‑1 (MQ=255)

                                                                                                 aTAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGACAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTATCTGACTGATACTGTCa                                   >  2:3001766/1‑140 (MQ=255)

                                                                                                        gTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAGTAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGcc                            >  2:3847482/1‑140 (MQ=255)

                                                                                                        gTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGcc                            >  1:1329894/1‑140 (MQ=255)

                                                                                                         ttGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAAAAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCCTCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGcac                           >  2:757124/1‑138 (MQ=255)

                                                                                                          tGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGcccc                          >  2:2926148/1‑140 (MQ=255)

                                                                                                            tataCTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGc                        >  1:4559854/1‑140 (MQ=255)

                                                                                                                 tttCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCg                   >  1:2061101/1‑140 (MQ=255)

                                                                                                                 tttCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCg                   >  2:3195009/1‑140 (MQ=255)

                                                                                                                   tCTTGCATGCGCGTTTCCGGCGCTAAACGTGGGGATGATAGGCGAACAGCGCGGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCCTCATCTTCGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCt                 >  2:668901/1‑140 (MQ=255)

                                                                                                                   tCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGCTGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTCCATCTTTGAAGCGTTTCTGACTGATACCGTGAATCAGCCCCGCCATCGCt                 >  1:3771696/1‑140 (MQ=255)

                                                                                                                   tCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCt                 >  1:726858/1‑140 (MQ=255)

                                                                                                                   tCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCt                 >  1:1655527/1‑140 (MQ=255)

                                                                                                                   tCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCt                 >  1:2668263/1‑140 (MQ=255)

                                                                                                                   tCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCt                 >  1:2717606/1‑140 (MQ=255)

                                                                                                                   tCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCt                 >  2:3402154/1‑140 (MQ=255)

                                                                                                                   tCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCt                 >  2:3326816/1‑140 (MQ=255)

                                                                                                                   tCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGAGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTGATCTTTGAAGCGTTTCTGACTGATACCGGCAATCAGCCCCGCCATCGCt                 >  2:1059504/1‑140 (MQ=255)

                                                                                                                    cTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtt                <  2:4855227/140‑1 (MQ=255)

                                                                                                                    cTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtt                >  2:4189663/1‑140 (MQ=255)

                                                                                                                     ttGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGC‑CCGCCGGTATGAATAAACAGAACCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt              <  2:2486846/140‑1 (MQ=255)

                                                                                                                      tGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt              <  2:1396734/140‑1 (MQ=255)

                                                                                                                      tGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt              <  2:4157689/140‑1 (MQ=255)

                                                                                                                      tGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt              <  1:3644345/140‑1 (MQ=255)

                                                                                                                      tGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt              <  1:3087707/140‑1 (MQ=255)

                                                                                                                      tGCATGAGCGTTTCCGGAGCTAAACGTGGCGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCATCCCCGCCATCGCtttt              <  2:3577035/140‑1 (MQ=255)

                                                                                                                        cATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTcc            >  1:1798960/1‑140 (MQ=255)

                                                                                                                         aTGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCg           <  1:2167246/140‑1 (MQ=255)

                                                                                                                         aTGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCg           <  1:208596/140‑1 (MQ=255)

                                                                                                                           gAGCGTTTCCGGAGCTAAACGTGTGGATTCTAGTCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCCATCAGCCCCGCCACCGCTTTTCCGGt         <  2:5064199/140‑1 (MQ=255)

                                                                                                                           gAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGt         <  2:3993787/140‑1 (MQ=255)

                                                                                                                           gAGCGTTTCCGAAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCATTCATCTTTGCAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGt         <  2:1352636/140‑1 (MQ=255)

                                                                                                                             gCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGtat       >  1:2220791/1‑140 (MQ=255)

                                                                                                                             gCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTAGGAATAAACAGAATCGGACCTTCATCTGTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTGCCGGtat       >  1:4266054/1‑140 (MQ=255)

                                                                                                                                tttCCGGAGCTACACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCATTCATCTTTGAAGCGTTTCTGACTGATACCGTCCATCAGCCCCGCCATCGCTTTTCCGGTATaca    <  1:3906566/140‑1 (MQ=255)

                                                                                                                                tttCCGGAGCTAAACGTGGGGATGATAGGCGTACAGCGCACGCGCACCGCCGGTATGAAGAAACAGGATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATaca    <  2:871712/140‑1 (MQ=255)

                                                                                                                                tttCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATaca    <  1:4050800/140‑1 (MQ=255)

                                                                                                                                tttCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATaca    <  1:3248578/140‑1 (MQ=255)

CGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACA  >  NZ_CP025268/2002397‑2002666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`