BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,224,824	A→G	C401R (TGT→CGT)	bglX ←	beta‑glucosidase BglX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,224,824	0	A	G	99.4%	1088.5 / NA	325	C401R (TGT→CGT)	bglX	beta‑glucosidase BglX
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (128/195); minor base C (0/2); total (128/197)
Fisher's exact test for biased strand distribution p-value = 5.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 325 total aligned reads displayed.

AATCCCGGTCAGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACAGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCCATA  >  NZ_CP025268/2224687‑2224960

 aTCCCGGTCAGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGtt                                                                                                                                       >  2:1071177/1‑140 (MQ=255)

      ggTCAGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGTGACGGTTTTTCa                                                                                                                                  >  2:3608178/1‑140 (MQ=255)

       gTCAGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAg                                                                                                                                 >  1:2068057/1‑140 (MQ=255)

          aGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCaa                                                                                                                              <  1:2382378/140‑1 (MQ=255)

                 tCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAg                                                                                                                       >  1:1033183/1‑140 (MQ=255)

                 tCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAg                                                                                                                       >  2:1376229/1‑140 (MQ=255)

                    cGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCtt                                                                                                                    >  1:1773384/1‑140 (MQ=255)

                      gATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTc                                                                                                                  >  2:2259880/1‑140 (MQ=255)

                      gATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTc                                                                                                                  >  1:199589/1‑140 (MQ=255)

                      gATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTc                                                                                                                  >  2:603568/1‑140 (MQ=255)

                      gATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTc                                                                                                                  >  1:5085236/1‑140 (MQ=255)

                        ttGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTAAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTcgc                                                                                                                <  2:75849/140‑1 (MQ=255)

                           aTCGGCAACACCGGCTGCGGCCCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTcgcgcg                                                                                                             <  1:4264811/140‑1 (MQ=255)

                           aTCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCCGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGGTTCGAGACGGTTTTTCAGCAACACCAAGCTCTcgcgcg                                                                                                             >  2:4861190/1‑140 (MQ=255)

                           aTCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTGGAGACGGTTTTTCAGCAACACCAAGCTTTcgcgcg                                                                                                             >  1:3146851/1‑140 (MQ=255)

                           aTCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTcgcgcg                                                                                                             >  1:802538/1‑140 (MQ=255)

                           aTCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTcgcgcg                                                                                                             >  1:3835892/1‑140 (MQ=255)

                           aTCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTcgcgcg                                                                                                             >  1:1058175/1‑140 (MQ=255)

                           aTCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTcgcgcg                                                                                                             >  1:3517907/1‑140 (MQ=255)

                           aTCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTcgcgcg                                                                                                             >  1:3421301/1‑140 (MQ=255)

                           aTCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTcgcgcg                                                                                                             >  1:301891/1‑140 (MQ=255)

                           aTCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTcgcgcg                                                                                                             >  1:2290783/1‑140 (MQ=255)

                           aTCGGCAACACCGGCTGCGGACCAGCCGCCCAGCACGTCACGGTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATGTTTTCAGCGGTAACGTTTCGAGACGGTCTTTCATCAACACCAAGCTTTctcgcc                                                                                                             >  2:696364/1‑139 (MQ=255)

                            tCGGCAACACCGGCTGTGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGGCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCgcgcgc                                                                                                            <  1:2839033/140‑1 (MQ=255)

                               gCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCAc                                                                                                         >  2:2465746/1‑140 (MQ=255)

                                     ccGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCAcg                                                                                                   <  2:1404301/140‑1 (MQ=255)

                                     ccGGCTGCGCACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCAcg                                                                                                   <  1:3662083/140‑1 (MQ=255)

                                     ccGGCTCCGGACCAGCTGCCCATCACGTCACGTTTACTGCCCGCCAGTGGCCCAACCCCCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCCGCAACACCAAGCTTTCGCGCGCCACTTCAcg                                                                                                   <  2:2679437/140‑1 (MQ=255)

                                     caggCAGCGGACCAGCTGCCCATCCCGACACGTTTACTGTCCGCCAGTCGCCCAACCACCGCAATGGAGGCCGAATTTATAAGCGGTAAACTATCGAGACGCTTATTCAGCAACACCAAGCTATCACGCTCTACTTAAcc                                                                                                   >  2:1210567/3‑139 (MQ=255)

                                       ggCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCAcgcg                                                                                                 <  2:3676216/140‑1 (MQ=255)

                                       ggCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCAcgcg                                                                                                 >  1:2873043/1‑140 (MQ=255)

                                       ggCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCAcgcg                                                                                                 <  2:2496130/140‑1 (MQ=255)

                                       ggCTGCGGACCAGCGGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCAcgcg                                                                                                 <  2:3771094/140‑1 (MQ=255)

                                        gCTGCCGATCTGCTGCCCATCACGTCACGTTTACTCGCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGTGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACgcgc                                                                                                <  2:3734878/140‑1 (MQ=255)

                                          tGCGGCCCAGCTTCACATCACGCCACGTTTACTGACCGCCAGTGGCACAACCACCGCAATGGTGGCCGATTTTTTACGCGGTAACGTTTCGAGACGGTTTTTCAGCAACCCCAAGCTTTCGCGCGCCACTTCACGCGctt                                                                                              <  2:112805/140‑1 (MQ=255)

                                          tGCGGACCAGCTGCCCATCCCGTCACGTTTCCTGACCGCCAGTGGCCCAACCCACGAAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGCGCCGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGctt                                                                                              <  2:1988844/140‑1 (MQ=255)

                                               aCCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTGTTCCGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTa                                                                                         <  1:1474917/140‑1 (MQ=255)

                                                   gCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGt                                                                                     <  2:2888558/140‑1 (MQ=255)

                                                   gCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGt                                                                                     <  1:401081/140‑1 (MQ=255)

                                                      gCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCGTCTTTACGGTGCa                                                                                  >  2:117032/1‑140 (MQ=255)

                                                         cATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAggc                                                                               >  2:1796976/1‑140 (MQ=255)

                                                             acgtcacgtTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCt                                                                           <  1:485954/140‑1 (MQ=255)

                                                             acgtcacgtTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCt                                                                           <  1:2764068/140‑1 (MQ=255)

                                                               gtcacgtTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCCGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTGACGGTGCAGGCGGCttt                                                                         <  1:770449/140‑1 (MQ=255)

                                                               gtcacgtTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCttt                                                                         >  2:302916/1‑140 (MQ=255)

                                                               gtcacgtTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCttt                                                                         <  2:475722/140‑1 (MQ=255)

                                                                 cacgtTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCg                                                                       >  1:3772849/1‑140 (MQ=255)

                                                                    gtTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCa                                                                    <  1:2668031/140‑1 (MQ=255)

                                                                    gtTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCa                                                                    <  1:2320357/140‑1 (MQ=255)

                                                                    gtTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCa                                                                    <  1:2339399/140‑1 (MQ=255)

                                                                     tttACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCAt                                                                   <  1:2951200/140‑1 (MQ=255)

                                                                     tttACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGGTTTTCAGCAACACCAAGCTTTCGCGCGCCACGTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCAt                                                                   >  2:4134262/1‑140 (MQ=255)

                                                                          tGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTa                                                              <  2:4386064/140‑1 (MQ=255)

                                                                            tCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATc                                                            <  2:4646060/140‑1 (MQ=255)

                                                                            tCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATc                                                            >  1:876915/1‑140 (MQ=255)

                                                                             ccGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGCCGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTGCTTTACGGTGCAGGCGGCTTTCGGCCTTGGTATcc                                                           >  2:2077669/1‑140 (MQ=255)

                                                                              cGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCa                                                          <  1:4355405/140‑1 (MQ=255)

                                                                              cGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCGTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCa                                                          >  1:4073948/1‑140 (MQ=255)

                                                                               gCCAGTGGCCCAACCACCGCAATGGTGGCCGATGTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCAc                                                         <  2:157836/140‑1 (MQ=255)

                                                                                ccAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCAcc                                                        <  1:1877792/140‑1 (MQ=255)

                                                                                ccAGGGTCCCATCGACCGCAAGGGTGGCCGATTTTTTCGGCGGTCACGTTTCGAGACGGTTTTTCAGCACCACCGCGCTTTCGCGCGCCACTCCACCCGCTTCTTTACGGTTCAGGCGGCTTTCGGCATTGGTATCCAcc                                                        <  2:3442696/140‑1 (MQ=255)

                                                                                      gCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTcc                                                  >  2:2858196/1‑139 (MQ=255)

                                                                                           accaccGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGActc                                             >  2:1523185/1‑140 (MQ=255)

                                                                                              accGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCttt                                          <  1:2259070/140‑1 (MQ=255)

                                                                                                cGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCg                                        >  2:4333512/1‑140 (MQ=255)

                                                                                                cGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCg                                        >  2:3987219/1‑140 (MQ=255)

                                                                                                 gCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGTTATCCACCGGGTCAGACTCTTTCgg                                       >  1:1713553/1‑140 (MQ=255)

                                                                                                 gCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCgg                                       <  1:4628262/140‑1 (MQ=255)

                                                                                                      ggtggCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCa                                  <  1:3807100/140‑1 (MQ=255)

                                                                                                      ggtggCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCa                                  <  2:4414190/140‑1 (MQ=255)

                                                                                                      ggtggCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCa                                  <  1:155255/140‑1 (MQ=255)

                                                                                                      ggtggCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCa                                  <  1:1379646/140‑1 (MQ=255)

                                                                                                       gtggCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCaa                                 >  2:2612573/1‑140 (MQ=255)

                                                                                                        tggCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCaaa                                <  1:4590323/140‑1 (MQ=255)

                                                                                                         ggCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAAt                               <  1:953084/140‑1 (MQ=255)

                                                                                                         gcccGATTTGTTCAGCGGTAACGTTTCGAGACGGTTTTTCCGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAAt                               <  2:3180397/138‑1 (MQ=255)

                                                                                                          gCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATg                              >  2:214885/1‑140 (MQ=255)

                                                                                                          gCCGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATg                              >  1:4792871/1‑140 (MQ=255)

                                                                                                          gCCGATTTTTTCAGCGGGAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATg                              >  2:4244734/1‑140 (MQ=255)

                                                                                                           ccGATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATgg                             <  2:3853681/140‑1 (MQ=255)

                                                                                                             gATTTTTTCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCt                           <  1:432127/140‑1 (MQ=255)

                                                                                                                  tttCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATg                      <  1:2457618/140‑1 (MQ=255)

                                                                                                                  tttCAGCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATg                      <  2:524692/140‑1 (MQ=255)

                                                                                                                  tttCAGCGGTAACGTTTCCAGACCGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATg                      <  2:4098429/140‑1 (MQ=255)

                                                                                                                       gCGGTAACGTTTCGAGACGGTTTTTCGGCAACACCAAGCTTTCGGGCGCCAGTTCACGCGCTTCTTTACGGGGCAGGCGGCGTTCGGCATTGGTATCGACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCg                 <  2:5019169/140‑1 (MQ=255)

                                                                                                                       gCGGTAACGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCg                 <  1:3350326/140‑1 (MQ=255)

                                                                                                                            aaCGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTaaa            <  1:1221228/140‑1 (MQ=255)

                                                                                                                            aaCGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTaaa            <  2:3504431/140‑1 (MQ=255)

                                                                                                                            aaCGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTaaa            <  1:5032653/140‑1 (MQ=255)

                                                                                                                            aaCGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTaaa            <  1:4550077/140‑1 (MQ=255)

                                                                                                                             aCGTTTCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCAACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAaac           <  1:2736173/140‑1 (MQ=255)

                                                                                                                                 ttCTAGACGTTTTTTCAGCAACACCTAGCTTTCGTCCCCCACTTACCGCGCTTCTTTACGTTGCTGGCGGTTTGCGCCATCAGTATCCACCGGTTCAGTCTCATTCGGCCCCCAATAGCTGTATGGGTCGTTAAACAAcc       <  2:2375955/140‑1 (MQ=255)

                                                                                                                                 ttCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAAcc       <  1:2102006/140‑1 (MQ=255)

                                                                                                                                 ttCGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAAcc       <  1:3554394/140‑1 (MQ=255)

                                                                                                                                   cGAGACGGTTTTTCATCAACCCCAAGCCTTCGCGTGCCCCTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCACACTCTTTCGGCCCCAAATGGCTTTATGGGTCGTTAAACAAcccc     <  2:1843243/140‑1 (MQ=255)

                                                                                                                                   cGAGACGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAAcccc     <  1:2397969/140‑1 (MQ=255)

                                                                                                                                     agaCGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCACATGGCTGTATGGGTCGTTAAACAACCCcat   >  1:528600/1‑140 (MQ=255)

                                                                                                                                     agaCGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCcat   >  2:3319877/1‑140 (MQ=255)

                                                                                                                                     agaCGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCcat   >  2:1762656/1‑140 (MQ=255)

                                                                                                                                      gaCGGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGTTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCcata  <  2:3012832/140‑1 (MQ=255)

AATCCCGGTCAGTACGGTCACGGATTGATCGGCAACACCGGCTGCGGACCAGCTGCCCATCACGTCACGTTTACTGTCCGCCAGTGGCCCAACCACCGCAATGGTGGCCGATTTTTTCAGCGGTAACGTTTCGAGACAGTTTTTCAGCAACACCAAGCTTTCGCGCGCCACTTCACGCGCTTCTTTACGGTGCAGGCGGCTTTCGGCATTGGTATCCACCGGGTCAGACTCTTTCGGCCCCAAATGGCTGTATGGGTCGTTAAACAACCCCATA  >  NZ_CP025268/2224687‑2224960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`