BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,267,514	C→T	V6V (GTG→GTA)	fruB ←	fused PTS fructose transporter subunit IIA/HPr protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,267,514	0	C	T	100.0%	867.9 / NA	234	V6V (GTG→GTA)	fruB	fused PTS fructose transporter subunit IIA/HPr protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (86/148); total (86/148)

Only 100 of 234 total aligned reads displayed.

TGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAGCATAATACTT  >  NZ_CP025268/2267379‑2267647

  aGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGAt                                                                                                                                 <  1:2317100/140‑1 (MQ=255)

  aGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGAt                                                                                                                                 <  1:861360/140‑1 (MQ=255)

    gTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATaa                                                                                                                               <  1:4525901/140‑1 (MQ=255)

    gTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAAGCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATaa                                                                                                                               <  1:1905213/140‑1 (MQ=255)

     tttGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAAc                                                                                                                              <  1:2881800/140‑1 (MQ=255)

     tttGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAAc                                                                                                                              <  1:3612290/140‑1 (MQ=255)

       tGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTg                                                                                                                            >  1:100551/1‑140 (MQ=255)

       tGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTg                                                                                                                            <  2:1529709/140‑1 (MQ=255)

         cTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGACCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGa                                                                                                                          >  2:327241/1‑140 (MQ=255)

            ttggcgcgcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACa                                                                                                                       <  2:158918/137‑1 (MQ=255)

            ttCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACa                                                                                                                       <  1:4909996/140‑1 (MQ=255)

            ttCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACa                                                                                                                       <  1:4041210/140‑1 (MQ=255)

              cgcgcgCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATa                                                                                                                     <  1:543682/140‑1 (MQ=255)

               gcgcgcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAg                                                                                                                    <  2:955865/140‑1 (MQ=255)

                  cgcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTtc                                                                                                                 <  2:2987511/140‑1 (MQ=255)

                  cgcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTtc                                                                                                                 <  1:3192444/140‑1 (MQ=255)

                    cgaGCATGCCATTGCCGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCGTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTtctc                                                                                                               <  1:5026632/138‑1 (MQ=255)

                            ccATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTg                                                                                                       <  2:4528238/140‑1 (MQ=255)

                            ccATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTg                                                                                                       <  1:62985/140‑1 (MQ=255)

                             cATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGCCCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTCGAACATAGTTCTCCTCTCTTGc                                                                                                      <  2:2127489/140‑1 (MQ=255)

                             cATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGc                                                                                                      >  2:4388482/1‑140 (MQ=255)

                             cATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGc                                                                                                      >  1:3005391/1‑140 (MQ=255)

                              aTTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCt                                                                                                     <  2:4927584/140‑1 (MQ=255)

                              aTTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCt                                                                                                     <  2:4923153/140‑1 (MQ=255)

                               ttGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTg                                                                                                    >  1:2653547/1‑140 (MQ=255)

                                 gACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGaa                                                                                                  >  2:1014548/1‑140 (MQ=255)

                                  aCGTAGCCTTCTGCTACCTTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAGCATAGTTCTCCTCTCTTGCTGAAt                                                                                                 <  2:2345503/140‑1 (MQ=255)

                                  aCGTAGCCTTCTGCTACATTACCGGCCTGCATCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTCTCTCCGGCCTTTTCGCCCTGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAt                                                                                                 >  2:2742849/1‑140 (MQ=255)

                                    gTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTg                                                                                               >  1:3310980/1‑140 (MQ=255)

                                      aGCCTTCTTCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaa                                                                                             <  2:2048671/140‑1 (MQ=255)

                                      aGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaa                                                                                             >  2:392617/1‑140 (MQ=255)

                                      aGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaa                                                                                             <  1:121231/140‑1 (MQ=255)

                                      aGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaa                                                                                             <  1:4725213/140‑1 (MQ=255)

                                       gCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaaa                                                                                            >  1:3948514/1‑140 (MQ=255)

                                       gCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaaa                                                                                            >  2:849690/1‑140 (MQ=255)

                                       gCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaaa                                                                                            >  2:2760991/1‑140 (MQ=255)

                                       gCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTCTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaaa                                                                                            >  1:4145754/1‑140 (MQ=255)

                                        ccTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATTTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAAc                                                                                           >  1:435848/1‑140 (MQ=255)

                                        ccTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAAc                                                                                           <  2:2974320/140‑1 (MQ=255)

                                            cTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGAtt                                                                                       >  1:2047301/1‑140 (MQ=255)

                                            cTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCGTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGAtt                                                                                       >  1:237362/1‑140 (MQ=255)

                                            cTGCTACATTACCGGCCTGCACCAACGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCGCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGAtt                                                                                       >  1:1492791/1‑140 (MQ=255)

                                             tGCTACATTACCGGCCTGCACCAGCTCCGCAGCGACCGGGCGAAGCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGGCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTTCTGAATTGaacgcattc                                                                                      >  2:128289/1‑133 (MQ=255)

                                                tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGc                                                                                   <  1:1437920/140‑1 (MQ=255)

                                                  cATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTGTCATCTCTTGCTGAATTGCAACGATTCAGCct                                                                                 >  2:4227795/1‑140 (MQ=255)

                                                  cATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCct                                                                                 >  1:4865758/1‑140 (MQ=255)

                                                      aCCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTAt                                                                             <  1:1117268/140‑1 (MQ=255)

                                                      aCCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTAt                                                                             <  1:1117357/140‑1 (MQ=255)

                                                       ccGGCCTGCACCAGCGCCGCAGCGACCTGGCGACTCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGTATGTCCTTTACGGCTAACTGGAACATATTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATg                                                                            >  1:2546701/1‑140 (MQ=255)

                                                          gCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATgaga                                                                         >  2:1111897/1‑140 (MQ=255)

                                                              gCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGaaaaa                                                                     <  1:2099553/140‑1 (MQ=255)

                                                               cACCAGCGGCGCACCGACCTGGCGAATCGACTCTTATTTGTCTCCGTCATTTTCGCCCGGATGGATGTCCTGTACGGATAAGTGGAACATAGTTCTCCTCTCTTGCTTAATTGAAACGATTCAGCCTCTATGAGaaaaaa                                                                    >  2:531669/1‑140 (MQ=255)

                                                               cACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGaaaaaa                                                                    >  1:956937/1‑140 (MQ=255)

                                                               cACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTCGCTGAATTGAAACGATTCAGCCTCTATGAGaaaaaa                                                                    >  1:2577733/1‑140 (MQ=255)

                                                                   cgcgcCGCAGCGCCCTGGCGCATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAgcg                                                                <  2:1855800/139‑1 (MQ=255)

                                                                   aGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATAGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAgcg                                                                <  1:4253449/140‑1 (MQ=255)

                                                                     cgccgcAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAAc                                                                                           >  2:3788433/1‑111 (MQ=255)

                                                                     cgccgcAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAAc                                                                                           <  1:3788433/111‑1 (MQ=255)

                                                                          cAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGCTGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCt                                                         >  2:144754/1‑140 (MQ=255)

                                                                          cAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCt                                                         >  1:4704619/1‑140 (MQ=255)

                                                                          cAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCt                                                         >  1:1366078/1‑140 (MQ=255)

                                                                              taCCTGGCTACTGGCCCCTTCTTTGCCACCGCCGTGGTCGCCCGGATGTATGTCCTGTCCTGATAACTGGAACATAGTTCTACTCTCAGGCTGAATTGAATCGCTCCAGCCCCTCTGAGACCAAAAGCGCCAACCTGGCt                                                     <  2:100171/139‑1 (MQ=255)

                                                                              gACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCt                                                     <  1:2173595/140‑1 (MQ=255)

                                                                              gACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCt                                                     <  1:274963/140‑1 (MQ=255)

                                                                              gACCTGGCGAAGCGCCTCTTCTTTGTCTCCGGACTTTTCGCCAGGATGGATGGCCTGTACGGATAAATGGAACATAGTTCGCCGCTCTTGCTGAAGTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCt                                                     <  2:4858330/140‑1 (MQ=255)

                                                                               aCCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCtt                                                    >  1:1785357/1‑140 (MQ=255)

                                                                               aCCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCtt                                                    <  2:3571659/140‑1 (MQ=255)

                                                                                ccTGGCGACCTGCCTCTTCTTAGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTTCGGATAACTGGAACATAGTTCTCCTCTCTTGCTGCATTGAAACGATTCAGCCTCTCTGAGAAAAAAAGCGCCAACCTGGCCTa                                                   <  2:3747369/140‑1 (MQ=255)

                                                                                    gCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGt                                               >  1:3254887/1‑140 (MQ=255)

                                                                                     cGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGtt                                              <  2:3952723/140‑1 (MQ=255)

                                                                                     cGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGtt                                              <  1:2134886/140‑1 (MQ=255)

                                                                                     cGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGtt                                              <  1:3633583/140‑1 (MQ=255)

                                                                                     cGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGACGTCCTGTACGGATAACTGGAACCTAGTTCTCCCCCCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAACAAAGCGCCAACCTGGCTTCGGGtt                                              <  2:242100/140‑1 (MQ=255)

                                                                                     cGAATCGCCTCTTCTTTGTCTCCGGCCCTTTCGCCCGGATGGATGTCCTGTACGCATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGCTTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGtt                                              <  1:1549922/140‑1 (MQ=255)

                                                                                            ccTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACa                                       >  2:3854926/1‑140 (MQ=255)

                                                                                              tcttctTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGCCAAg                                     >  2:631697/1‑140 (MQ=255)

                                                                                               cttcttTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGa                                    <  1:3519282/140‑1 (MQ=255)

                                                                                                     tGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGGTAAAGACAAGATCgcgc                              >  1:1606503/1‑140 (MQ=255)

                                                                                                       tctcCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTg                            >  2:3187130/1‑140 (MQ=255)

                                                                                                        ctcCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGa                           <  1:2453307/140‑1 (MQ=255)

                                                                                                        ctcCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGa                           <  1:1928672/140‑1 (MQ=255)

                                                                                                          ccGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTTGCACATAGTTCTCCTCTCTTGCTGCATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCCGGCTTAGGGTTAAAGACAAGATCGCGCTGaaa                         >  2:2070124/1‑140 (MQ=255)

                                                                                                          ccGGCCTTTTCGACCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGaga                         >  2:5046830/1‑138 (MQ=255)

                                                                                                             cccctttCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAACCTCTATGCGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGt                      <  2:412310/136‑1 (MQ=255)

                                                                                                               cTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGttt                    <  1:1291601/140‑1 (MQ=255)

                                                                                                                 tttCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAACAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCa                  >  2:4406476/1‑140 (MQ=255)

                                                                                                                 tttCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCa                  <  2:2926129/140‑1 (MQ=255)

                                                                                                                 tttCGCCCGGATGGAGGTCCTGTACGTATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGTGTTAGGGTTAAAGACAAGATCGCTCTGAAACGGTTCa                  >  2:4267088/1‑140 (MQ=255)

                                                                                                                  ttCGCCCGGACGGATGTCCTGTATGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCaa                 <  2:3765992/140‑1 (MQ=255)

                                                                                                                    cGCCCGGATGGATGTCGTGTACGGATAACCGGAGCATAGTTCTCCCCTCTGGCTGAATTGAAACGATTCAGCCTCTATCAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGa               <  2:2664106/140‑1 (MQ=255)

                                                                                                                    cGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGa               <  1:4606342/140‑1 (MQ=255)

                                                                                                                    cGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGa               <  1:4781424/140‑1 (MQ=255)

                                                                                                                    cGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGa               <  1:130713/140‑1 (MQ=255)

                                                                                                                    cGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAGTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGa               <  1:3125445/140‑1 (MQ=255)

                                                                                                                    cGCCCGGATGGATGTCCTCTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGa               <  1:3108346/140‑1 (MQ=255)

                                                                                                                       ccGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAg            <  2:174456/140‑1 (MQ=255)

                                                                                                                          gatggatgTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATGGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAGCat         <  2:3310980/140‑1 (MQ=255)

                                                                                                                            tggatgTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAGCataa       <  1:410281/140‑1 (MQ=255)

                                                                                                                            tggatgTCCTGTACCGATAACTGGAACATAGTCCTCCTCTCTTGCTTAATTGAGACGATTCAGCCTCTATGAGAAAAAAAGCGCCAGCCTCGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAGCataa       <  1:3378257/140‑1 (MQ=255)

                                                                                                                                 gTCCTGTACGGATAACCGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAGCATAATACtt  <  2:1798918/140‑1 (MQ=255)

TGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGCACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTCAGCCTCTATGAGAAAAAAAGCGCCAACCTGGCTTAGGGTTAAAGACAAGATCGCGCTGAAACGTTTCAAGAAAGCATAATACTT  >  NZ_CP025268/2267379‑2267647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`