Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,293,062	A→G	intergenic (‑106/+38)	yejO ← / ← CXP41_RS12140	autotransporter YejO/IS5‑like element IS5 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,293,062	0	A	G	100.0%	142.0 / NA	53	intergenic (‑106/+38)	yejO/CXP41_RS12140	autotransporter YejO/IS5‑like element IS5 family transposase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (46/7);  total (46/7)

TTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAAACCGTTTCTTCGCCATTTAAGGCG  >  NZ_CP025268/2292926‑2293086                                                                                                                                         |                         ttGATGCCACTGCTATAGGTGAATATAAGTCAGGTCTGAATCAGCGAGGAAATTCTCCCCGGCGGCGACAGTCATTGCATTTATTCATGTTGGAGCCGATTCTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAcc                       >  2:688281/1‑140 (MQ=9) ttGATGCCAATGCTATGGGTGGCTATAAGGAAGGCGCGAATAAGCGGGGCAAGTCTTCTCGGCTGACTCAGTCATTTCAGTTCTTCATGTCTGAGCCGCTTTTTTCTCCCGTACATGCCTTGAATCAGCCGATTTAGAcc                       >  2:4124360/1‑140 (MQ=14) ttGATGCCAATGCTATGGGTGAATATCAGGAAGGTACCAATAACCTGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTTATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCCGCCTATTTAGAcc                       >  1:4576087/1‑140 (MQ=14) ttGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCGTGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAcc                       >  2:869562/1‑140 (MQ=255) ttGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAcc                       >  2:1371974/1‑140 (MQ=255) ttGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAcc                       >  1:1633914/1‑140 (MQ=255) ttGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAcc                       >  1:699472/1‑140 (MQ=255) ttGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAcc                       >  1:2192234/1‑140 (MQ=255) ttGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAcc                       >  1:3593198/1‑140 (MQ=255) ttGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAcc                       >  1:3295348/1‑140 (MQ=255) ttGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTGGAATCAGCCTATTTAGAcc                       >  1:954305/1‑140 (MQ=255)  tGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCg                      >  1:1328491/1‑140 (MQ=255)   gATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGt                     >  1:1952368/1‑140 (MQ=255)   gATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATCTAGACCGt                     >  2:412396/1‑140 (MQ=255)   gATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTCTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGt                     >  1:1952278/1‑140 (MQ=255)    aTGCCAATTCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAGTTTTTCTCCCCTAAATGCCCTCAATCAGCCTATTTAGAcccct                    >  1:2990259/1‑137 (MQ=14)    aTGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGtt                    >  1:4169667/1‑140 (MQ=255)    aTGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAAGTCTTCTCGGCTGACTCTGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTTGACCGtt                    >  2:3882565/1‑140 (MQ=255)    aTGCCAATGCTATGGGGGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGtt                    >  1:758500/1‑140 (MQ=255)      gCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTttc                  >  2:1340951/1‑140 (MQ=255)      gCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCACTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTttc                  >  1:3057090/1‑140 (MQ=255)        cAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTttctt                >  2:264519/1‑140 (MQ=255)        cAATGCTATGGGGGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGCCTCAGTCATTTCATTTCTTCATGTTTGCGCCGAGTTTTTCTCCCGTAAATGCCGTGCAGCAGCCTGTTTAGACCGTtgctt                >  2:2751647/1‑140 (MQ=14)         aaTGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTttcttc               >  1:3528811/1‑140 (MQ=255)          aTGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGGGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCg              <  1:4383656/140‑1 (MQ=255)          aTGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCg              >  2:4472606/1‑140 (MQ=255)          aTGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCg              <  2:663218/140‑1 (MQ=255)           tGCTATGGGTGAGTATAAGGAAGGTGCGAATAAGCGGGGGAATTATTCTCGGCTGTCCCAGTCATTTCATTTCTTCATGTTTGAGCCGCGTTTTTCTCCCGTAGATGCCGTGAATCAGCCTATGTAGACTGTTTCTTCGc             >  2:2397453/1‑140 (MQ=11)           tGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGc             >  2:3548521/1‑140 (MQ=255)           tGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGc             >  2:2756777/1‑140 (MQ=255)           tGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGc             >  2:2475099/1‑140 (MQ=255)           tGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGc             >  1:450504/1‑140 (MQ=255)           tGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGc             >  1:3414497/1‑140 (MQ=255)           tGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGc             >  1:3337980/1‑140 (MQ=255)           tGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGc             >  1:2302253/1‑140 (MQ=255)           tGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGc             >  1:2244889/1‑140 (MQ=255)           tGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGc             >  1:1614869/1‑140 (MQ=255)           tGCTATGGGTCAATATAAGGAACGTGCGAATAAGCGGGGAAATTCTTCTCGGCTCACTCAGTCCTTTCATTTCTTCATGTTTGAGCCGATTTTTTCGCCCGTAAATGCCTTGAATCACCCTATTTAGACCGTTTCTTCGc             <  2:1917863/140‑1 (MQ=11)            gCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTCAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGcc            <  1:1744737/140‑1 (MQ=255)             cTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCa           <  2:4588535/140‑1 (MQ=37)               aTGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCGATTGAGACCGTTTCTTCGCCAtt         >  2:89918/1‑140 (MQ=255)               aTGGGGGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTGTTCTCGGCTGCCTCAGTCATTTCATTTCCTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGCCCGTATCTTCGCCAtt         >  2:4910728/1‑140 (MQ=11)                tGGGTGAATATAAGGAATGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCAGTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAAGGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCAttt        >  2:2361311/1‑140 (MQ=255)                tGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCAttt        >  1:5114673/1‑140 (MQ=21)                tGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCAttt        >  1:1502592/1‑140 (MQ=21)                tGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGACAttt        >  2:4792473/1‑140 (MQ=19)                tGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATCTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCAttt        >  1:4567752/1‑140 (MQ=19)                 gggTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTa       <  1:1663252/140‑1 (MQ=18)                  ggTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTCTCTTCGCCATTTaa      >  2:2960638/1‑140 (MQ=18)                   gTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAg     <  1:4881864/140‑1 (MQ=17)                      aaTATAAGGAAGGTGCGAATAAGCGGGGTAATTCTTCTCGGCTGACTCAGTCATTTGATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGGCTTGGATCAGCCTATTTAGAGCGGTTCTTCGCCATTGAAGGCg  >  2:4035884/1‑140 (MQ=11)                      aaTATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTGTTCGCCATTTAAGGCg  >  2:133492/1‑140 (MQ=11)                      aaTATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCg  >  2:33862/1‑140 (MQ=1)                                                                                                                                         |                         TTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAAACCGTTTCTTCGCCATTTAAGGCG  >  NZ_CP025268/2292926‑2293086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑