BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	228,589	C→T	noncoding (2/116 nt)	rrf →	5S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	228,589	0	C	T	97.0%	428.1 / ‑1.3	170	noncoding (2/116 nt)	rrf	5S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base C (0/5); new base T (89/75); total (89/81)
Fisher's exact test for biased strand distribution p-value = 2.22e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 170 total aligned reads displayed.

TTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAAG  >  NZ_CP025268/228453‑228714

ttGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTgg                                                                                                                            <  1:2851388/140‑1 (MQ=18)

ttGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTgg                                                                                                                            <  1:2560838/140‑1 (MQ=18)

 ttaGCTAGCCGGTACTAATGAACCGTGAGGCTTAACCTTACAACTCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAAGCAGAACTCAGAAGCGGTCTGATAAAACAGAATTTGCTTggc                                                                                                                           <  2:2601347/138‑1 (MQ=11)

 tGAGCTTACCGGTACTAATGATCCGTGAGGCTTAACCTTACAACGCCGAAGCTGTGTTGGCTGATTGAGAGAAGATTTTCAGTCTGATACAGGATAAATCAGAACGCAGAAGCGGTCTGATAAACCATAATTTGCCTggc                                                                                                                           <  2:4439433/140‑1 (MQ=11)

 tGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTggc                                                                                                                           >  2:1511866/1‑140 (MQ=18)

   aGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTggcgg                                                                                                                         >  1:1530155/1‑140 (MQ=18)

        aCCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTa                                                                                                                    >  1:3416668/1‑140 (MQ=21)

        aCCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTa                                                                                                                    <  1:2799780/140‑1 (MQ=21)

        aCCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTa                                                                                                                    >  2:3682973/1‑140 (MQ=21)

        aCCGGTACTAAAGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATCGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGGATTTGCTTGGCGGCCGTa                                                                                                                    >  2:3645913/1‑140 (MQ=255)

             tACTAATGAACCGTGAGGCTTAATCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGcgcg                                                                                                               <  2:2606056/140‑1 (MQ=21)

             tACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAATATTTTCAGCCCGATACAGATTAAATCAGAACTCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGcgcg                                                                                                               <  2:4859710/140‑1 (MQ=255)

             tACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGcgcg                                                                                                               <  1:2570705/140‑1 (MQ=21)

             tACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGcgcg                                                                                                               <  1:4878182/140‑1 (MQ=21)

             tACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGcgcg                                                                                                               <  2:2174813/140‑1 (MQ=21)

              aCTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCgg                                                                                                              <  1:1705328/140‑1 (MQ=21)

              aCTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCgg                                                                                                              >  2:3897678/1‑140 (MQ=21)

              aCTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTGGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCgg                                                                                                              <  1:3901238/140‑1 (MQ=19)

               cTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCggt                                                                                                             >  1:4532859/1‑140 (MQ=21)

                  aTGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTATATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCggtggt                                                                                                          <  1:4316928/140‑1 (MQ=21)

                  aTGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAACACAGAATTTGCTTGGCGGCCGTAGCGCggtggt                                                                                                          <  2:314095/140‑1 (MQ=19)

                  aTGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCggtggt                                                                                                          <  1:1499054/140‑1 (MQ=21)

                  aTGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCggtggt                                                                                                          <  1:3929372/140‑1 (MQ=21)

                  aTGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCggtggt                                                                                                          >  2:1771509/1‑140 (MQ=21)

                  aTGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCggtggt                                                                                                          >  2:4821737/1‑140 (MQ=21)

                  aTGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCggtggt                                                                                                          >  2:4201816/1‑140 (MQ=21)

                  aTGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCggtggt                                                                                                          >  2:4182603/1‑140 (MQ=21)

                  aTGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCggtggt                                                                                                          >  2:4057557/1‑140 (MQ=21)

                  aTGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCggtggt                                                                                                          <  2:3841124/140‑1 (MQ=21)

                  aTGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCggtggt                                                                                                          >  2:4057527/1‑140 (MQ=21)

                  aTGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCggtggc                                                                                                          <  1:2992877/140‑2 (MQ=21)

                  aTGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGACTAAATCAGAACGCAGAAGCGGTCTGAGAAAACAGAATTTGCTTGGCGGCCGTAGCGCggaggt                                                                                                          >  2:3025500/1‑140 (MQ=255)

                  aTGAACCATGTGTCTCAACCGTACAACGCCGAAGCTGTTTTGGGGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCggtgct                                                                                                          >  2:4270526/1‑138 (MQ=11)

                   tGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTc                                                                                                         <  1:1284563/140‑1 (MQ=21)

                   tGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTc                                                                                                         <  2:1269974/140‑1 (MQ=21)

                   tGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTc                                                                                                         <  1:662944/140‑1 (MQ=21)

                   tGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTc                                                                                                         <  1:2704389/140‑1 (MQ=21)

                   tGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTc                                                                                                         <  1:3247850/140‑1 (MQ=21)

                   tGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTc                                                                                                         <  1:1434397/140‑1 (MQ=21)

                    gAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGAGTGAGAGAAGATTTTCAGCCGGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGGcc                                                                                                        <  2:361762/140‑1 (MQ=255)

                       ccGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATCAAACAGAATTTGCTTGTCGGCCGTAGCGCGGTGGTCCCAc                                                                                                     >  1:4691408/1‑140 (MQ=255)

                       ccGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCAc                                                                                                     >  1:2696812/1‑140 (MQ=21)

                       ccGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCAc                                                                                                     >  1:2486532/1‑140 (MQ=21)

                       ccGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCAc                                                                                                     >  1:2363843/1‑140 (MQ=21)

                       ccGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCAc                                                                                                     >  1:340131/1‑140 (MQ=21)

                       ccGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCAc                                                                                                     >  2:2090930/1‑140 (MQ=21)

                         gTGAGGCTTACCCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCt                                                                                                   <  1:4462849/140‑1 (MQ=21)

                         gTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCt                                                                                                   <  2:3567031/140‑1 (MQ=21)

                         gTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCt                                                                                                   <  2:3568355/140‑1 (MQ=21)

                          tGAGGCTTAACGTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAAGGCAGAAGCGGTCTCATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTg                                                                                                  >  1:4236122/1‑140 (MQ=255)

                          tGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTg                                                                                                  >  1:66026/1‑140 (MQ=21)

                          tGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTg                                                                                                  >  2:2890339/1‑140 (MQ=21)

                          tGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTg                                                                                                  >  2:2890195/1‑140 (MQ=21)

                          tGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTg                                                                                                  >  1:3312997/1‑140 (MQ=21)

                          tGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATCGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTg                                                                                                  >  1:1994646/1‑140 (MQ=12)

                            aGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGAc                                                                                                >  2:458978/1‑140 (MQ=21)

                             ggCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGAcc                                                                                               <  2:4415202/140‑1 (MQ=21)

                                  aaCCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATg                                                                                          >  1:2212301/1‑140 (MQ=21)

                                  aaCCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATg                                                                                          >  1:4727556/1‑140 (MQ=21)

                                  aaCCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATg                                                                                          >  1:41808/1‑140 (MQ=21)

                                  aaCCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATg                                                                                          <  1:3924838/140‑1 (MQ=21)

                                  aaCCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATg                                                                                          >  2:4376708/1‑140 (MQ=21)

                                  aaCCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATg                                                                                          >  2:1770855/1‑140 (MQ=21)

                                       tACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGaa                                                                                     <  2:3060038/140‑1 (MQ=21)

                                       tACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGaa                                                                                     >  2:71999/1‑140 (MQ=21)

                                       tACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGaa                                                                                     >  2:73038/1‑140 (MQ=21)

                                        aCAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAAc                                                                                    >  2:432774/1‑140 (MQ=21)

                                        aCAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAAc                                                                                    >  2:1173084/1‑140 (MQ=21)

                                        aCAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAAc                                                                                    >  1:4082554/1‑140 (MQ=21)

                                        aCAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAAc                                                                                    >  1:1594248/1‑140 (MQ=21)

                                         cAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACt                                                                                   >  2:5092688/1‑140 (MQ=21)

                                         cAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACt                                                                                   >  1:1706561/1‑140 (MQ=21)

                                         cAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACt                                                                                   >  1:5030446/1‑140 (MQ=21)

                                            cGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAg                                                                                <  2:959186/140‑1 (MQ=21)

                                            cGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAg                                                                                <  2:1703288/140‑1 (MQ=21)

                                            cGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAg                                                                                >  2:3320623/1‑140 (MQ=21)

                                            cGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATCTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCGGAACTCAg                                                                                <  2:2765438/140‑1 (MQ=255)

                                              ccGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACt                                                                                   >  2:3423111/1‑135 (MQ=21)

                                              ccGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACt                                                                                   <  1:3423111/135‑1 (MQ=21)

                                              ccGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGaa                                                                              >  1:187006/1‑140 (MQ=21)

                                              ccGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGaa                                                                              >  2:729866/1‑140 (MQ=21)

                                              ccGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGaa                                                                              >  1:970962/1‑140 (MQ=21)

                                               cGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAg                                                                             >  2:3113821/1‑140 (MQ=21)

                                               cGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAg                                                                             >  2:930098/1‑140 (MQ=21)

                                                 aaGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAACCAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTg                                                                           >  1:3112643/1‑140 (MQ=19)

                                                       ttttGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGc                                                                     >  2:684696/1‑140 (MQ=18)

                                                        tttGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGcc                                                                    <  1:3442287/140‑1 (MQ=18)

                                                         ttGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCg                                                                   <  1:4618437/140‑1 (MQ=18)

                                                           ggCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAGGCGATCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTa                                                                 <  1:3638646/140‑1 (MQ=12)

                                                           ggCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTa                                                                 <  1:1179254/140‑1 (MQ=18)

                                                           ggCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTa                                                                 <  1:506062/140‑1 (MQ=18)

                                                           ggCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTa                                                                 <  2:1362760/140‑1 (MQ=33)

                                                             cGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAgc                                                               <  2:2199443/140‑1 (MQ=18)

                                                             cGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAgc                                                               <  2:227649/140‑1 (MQ=18)

                                                                 ttGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCg                                                           >  1:754682/1‑140 (MQ=11)

                                                                 ttGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCg                                                           >  1:495069/1‑140 (MQ=11)

                                                                     gataagaTTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATgg                                                       <  2:3499271/137‑1 (MQ=1)

                                                                                                           cacaaGCGGTCTGATAAAACAGAATCTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGACGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGcc                 <  2:673637/137‑1 (MQ=0)

                                                                                                                      tgctAAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAAt      <  2:3244091/137‑1 (MQ=1)

                                                                                                                          aaaaCAGAATTTGCTTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAAg  <  1:2936177/140‑1 (MQ=11)

TTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCCGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAAG  >  NZ_CP025268/228453‑228714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`