BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,453,263	T→C	274 (TAA→TAG)	yfcO ←	fimbrial adhesin YfcO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,453,263	0	T	C	99.6%	906.6 / NA	278	274 (TAA→TAG)	yfcO	fimbrial adhesin YfcO
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (100/176); total (101/177)
Fisher's exact test for biased strand distribution p-value = 3.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 278 total aligned reads displayed.

TGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTC  >  NZ_CP025268/2453126‑2453397

tGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTa                                                                                                                                      >  2:2105691/1‑140 (MQ=255)

    ggTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAAc                                                                                                                                  <  1:881866/140‑1 (MQ=255)

        aTGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCTGCGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGAGGCGAATGCCCCTTCCTGGTGTAACTAAGGGCTACAACTCAt                                                                                                                              >  2:4518771/1‑140 (MQ=255)

        aTGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCAt                                                                                                                              >  2:4097165/1‑140 (MQ=255)

        aTGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCAt                                                                                                                              <  1:4729514/140‑1 (MQ=255)

        aTGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCAt                                                                                                                              <  1:3595643/140‑1 (MQ=255)

        aTGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCAt                                                                                                                              >  1:1828158/1‑140 (MQ=255)

        aTGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGAAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCAt                                                                                                                              <  1:4379029/140‑1 (MQ=255)

        aTGCGGCGTTGTTGGTGTCGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCAt                                                                                                                              >  2:4465825/1‑140 (MQ=255)

           cggcgTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCa                                                                                                                           <  1:1895001/140‑1 (MQ=255)

              cgTTGTTGGTGTTGATTGAAGTCGAGGTGTGGCTGCGGCCGGAGTTGGCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTa                                                                                                                        <  2:5026215/140‑1 (MQ=255)

              cgTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTa                                                                                                                        <  1:3227710/140‑1 (MQ=255)

              cgTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTa                                                                                                                        <  1:3006817/140‑1 (MQ=255)

              cgTTGTTGGTGTTGATTGAAGTCGAAAAGTCGCTGCCGCCGCGGTCGCCCTGAGGAGTTGCGCGCCTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTa                                                                                                                        <  2:1668266/140‑1 (MQ=255)

              cgTGGTTGGTGTTGATTGAAGTCGAAGGGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCGGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTa                                                                                                                        <  2:2990612/140‑1 (MQ=255)

                   ttgGTGTTGATTGATGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGATTGCCCCTGCCTGGTGTACATAAGGGCTACAACTCAGTCAGTATGTAc                                                                                                                   <  2:1259330/140‑1 (MQ=255)

                   ttgGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTAc                                                                                                                   <  1:4601844/140‑1 (MQ=255)

                        gtTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGaa                                                                                                              >  1:2911540/1‑140 (MQ=255)

                        gtTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGCACTGGaa                                                                                                              >  2:1078593/1‑140 (MQ=255)

                          tgattgaAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCTTTGGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAAcc                                                                                                            >  2:2239492/1‑140 (MQ=255)

                          tgattgaAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCTCCAGGTACGGGAAAGGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAATGGCTACAACCCATTCAGTATGTACTGGAAcc                                                                                                            >  2:4654963/1‑140 (MQ=255)

                           gattgaAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCg                                                                                                           >  1:4895706/1‑140 (MQ=255)

                             ttgaAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGc                                                                                                         <  2:3700998/140‑1 (MQ=255)

                             ttgaAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGGAGTGGAACCGGc                                                                                                         >  2:2340433/1‑140 (MQ=255)

                                 aGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGt                                                                                                     >  1:58438/1‑140 (MQ=255)

                                     gAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTa                                                                                                                        >  2:5003503/1‑117 (MQ=255)

                                     gAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTa                                                                                                                        <  1:5003503/117‑1 (MQ=255)

                                     gAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGaa                                                                                                 >  2:4953919/1‑140 (MQ=255)

                                         agTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGG‑CACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGAt                                                                                            <  1:2459604/140‑1 (MQ=255)

                                          gTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGAt                                                                                            <  1:3422183/140‑1 (MQ=255)

                                          gTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGAt                                                                                            <  1:3553013/140‑1 (MQ=255)

                                          gTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGAt                                                                                            <  1:4674183/140‑1 (MQ=255)

                                             gCTGCCGCCGGAGTTGCCCTGAGGAGTTGGGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCCAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTgg                                                                                         <  2:497037/140‑1 (MQ=255)

                                             gCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCCAGTAGAATCGATTgg                                                                                         >  2:2448896/1‑140 (MQ=255)

                                             gCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGAt                                                                                            >  2:2198937/1‑137 (MQ=255)

                                             gCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGAt                                                                                            <  1:2198937/137‑1 (MQ=255)

                                             gCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTgg                                                                                         >  2:2806726/1‑140 (MQ=255)

                                             gCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTgg                                                                                         >  1:433723/1‑140 (MQ=255)

                                                  cgccgGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCa                                                                                    <  1:1579468/140‑1 (MQ=255)

                                                   gccgGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCAt                                                                                   >  1:3860245/1‑140 (MQ=255)

                                                   gccgGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCAt                                                                                   >  1:2600178/1‑140 (MQ=255)

                                                    ccgGAGTTGCCCTGAGGAGTTGGGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATa                                                                                  <  2:1497125/140‑1 (MQ=255)

                                                    ccgGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATa                                                                                  <  1:2409267/140‑1 (MQ=255)

                                                        aGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATActgc                                                                              <  1:799992/140‑1 (MQ=255)

                                                            gCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGt                                                                          <  2:1293969/140‑1 (MQ=255)

                                                                 gaggagTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCaa                                                                     <  2:4323426/140‑1 (MQ=255)

                                                                     aggtGAGCGCGTTGCGCCGGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCGGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacg                                                                 <  2:3648373/137‑1 (MQ=255)

                                                                     agTTGCGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacg                                                                 <  2:4807384/140‑1 (MQ=255)

                                                                     agTTGCGCGCGTTGCGACAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacg                                                                 <  2:1743957/140‑1 (MQ=255)

                                                                     agTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacg                                                                 <  2:2881845/140‑1 (MQ=255)

                                                                     agTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacg                                                                 <  1:539497/140‑1 (MQ=255)

                                                                     agTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacg                                                                 <  1:1662303/140‑1 (MQ=255)

                                                                       ttGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTCTCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacgca                                                               <  2:238332/140‑1 (MQ=255)

                                                                       ttGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAAcacgca                                                               <  1:1995970/140‑1 (MQ=255)

                                                                            cgcgTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGAcc                                                          >  2:2005647/1‑140 (MQ=255)

                                                                             gcgTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCa                                                         <  1:2818136/140‑1 (MQ=255)

                                                                             gcgTTGCGCCAGGTACGGGTAATGGCAGGGCCGAATCCCCCTGCCTGGTGTATCTAACGGCTACAACTCATTCAGTATGTACTGGAACCGCCAAGTAGAATCGATTGGCCGCATACTGCTCGTATCAACACGCACGACCa                                                         <  2:4035343/140‑1 (MQ=255)

                                                                               gTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGACCCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCata                                                       <  1:1366336/140‑1 (MQ=255)

                                                                               gTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCata                                                       >  1:2629932/1‑140 (MQ=255)

                                                                               gTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCata                                                       <  1:2563472/140‑1 (MQ=255)

                                                                               gTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCata                                                       <  1:2299192/140‑1 (MQ=255)

                                                                               gTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCata                                                       <  1:1015086/140‑1 (MQ=255)

                                                                               gTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTCGCTGCATACTGCTGGTATCAACACGCACGACCata                                                       <  2:1795716/140‑1 (MQ=255)

                                                                               gTTGAGCCTGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGAGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCACCACGCACGACCata                                                       <  2:473170/140‑1 (MQ=255)

                                                                                 tGCGTCAGGTACGGGTGATGGCAGGGGCGAATGGCCCTGCCTGGTGTATCTAGGGGTTACAACGTATTCAGTATGTACTGGAACCGGCAAGTTGAATGGTTTGGCTGCATATTGTGGGTTTTGACACGCATGActttgtc                                                     >  2:599615/1‑134 (MQ=255)

                                                                                 tGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGGATCAACACGCACGACCATATc                                                     >  1:1184687/1‑140 (MQ=255)

                                                                                     ccAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCgtgt                                                 <  1:4492644/140‑1 (MQ=255)

                                                                                      cAGGTACGGGTAATCGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTa                                                <  2:2416557/140‑1 (MQ=255)

                                                                                           aCGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGt                                           <  2:917194/140‑1 (MQ=255)

                                                                                             gggTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGttt                                         <  2:2228631/140‑1 (MQ=255)

                                                                                             gggTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGttt                                         <  1:377172/140‑1 (MQ=255)

                                                                                             gggTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGttt                                         <  2:5065204/140‑1 (MQ=255)

                                                                                               gTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGt                                       <  2:1065601/140‑1 (MQ=255)

                                                                                               gTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGt                                       <  1:1085586/140‑1 (MQ=255)

                                                                                               gTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGt                                       <  1:3031845/140‑1 (MQ=255)

                                                                                               gTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGt                                       <  2:1828158/140‑1 (MQ=255)

                                                                                               gTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGt                                       <  1:4018955/140‑1 (MQ=255)

                                                                                               gTAATGGCAGGGGCGAATGCCCATGCCTGGTGTATCTAAGGGCGACAACTCATTCACTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGt                                       <  1:1930167/140‑1 (MQ=255)

                                                                                                   tGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTTCGt                                   >  1:1756175/1‑140 (MQ=255)

                                                                                                   tGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGt                                   >  1:988972/1‑140 (MQ=255)

                                                                                                    ggCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGGATGTACTGGAACCGGCAAGTAGAATCGAGTGGCTGCATACGGCTTGTAGCAACACGCACTACCATATCGTGTAACTGTTTGTCCGtt                                  >  2:154691/1‑140 (MQ=255)

                                                                                                     gCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTta                                 <  2:2671163/140‑1 (MQ=255)

                                                                                                     gCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTta                                 <  2:3181700/140‑1 (MQ=255)

                                                                                                       aGGGGCGAAGGGCACTGCGTGGTGTTGCTAATGGCTACAAATCTTTCAGTGTGTACTGGAAGCGGCAAGTAGAATCGATTGGCTGCATAGTGCTGGTATCAACACGCAGGTACATATCGTGTAACTGTTTGTCCGTtata                               <  2:74688/140‑1 (MQ=255)

                                                                                                        ggggCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAg                              >  1:3368892/1‑140 (MQ=255)

                                                                                                         gggCGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAgg                             >  1:3491279/1‑140 (MQ=255)

                                                                                                            cGAATGCCCCTGCCTGGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGgtg                          <  2:4386714/140‑1 (MQ=255)

                                                                                                                     ctgcctgGTGTATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTgg                 >  1:3667255/1‑140 (MQ=255)

                                                                                                                            gtgtATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTTGCGTGAcc          >  2:4000311/1‑140 (MQ=255)

                                                                                                                            gtgtATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGAcc          >  1:1480597/1‑140 (MQ=255)

                                                                                                                             tgtATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTCTCAACACGCACGACCATATCATGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCg         >  2:2931145/1‑140 (MQ=255)

                                                                                                                             tgtATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCg         <  1:4279945/140‑1 (MQ=255)

                                                                                                                             tgtATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCg         >  1:3190070/1‑140 (MQ=255)

                                                                                                                             tgtATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCg         >  2:4246478/1‑140 (MQ=255)

                                                                                                                             tgtATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACAATATCGTGTAACTGTTTGTCCGTAATAGGGTTGCGATTTGGCCTGACCg         >  2:136145/1‑140 (MQ=255)

                                                                                                                             tgtAGCTAAGGGCTACAACGCAGGCAGTATGGACGGGAACCGGCAAGTAGAATCGAGTGGCTGCATACTGCTGGTATCAACACGCACGACCAGATCGTGTAACGGGGTGTCCGTTATAGGGGGTCGAGTTGGCGTGACCg         >  2:3239560/1‑140 (MQ=255)

                                                                                                                              gtATCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCga        <  1:4110499/140‑1 (MQ=255)

                                                                                                                                 tCTAAGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGAc     >  1:2350806/1‑140 (MQ=255)

                                                                                                                                    aaGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTc  <  2:3798022/140‑1 (MQ=255)

                                                                                                                                    aaGGGCTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTc  <  2:3338890/140‑1 (MQ=255)

TGGCGGTGATGCGGCGTTGTTGGTGTTGATTGAAGTCGAAGAGTGGCTGCCGCCGGAGTTGCCCTGAGGAGTTGAGCGCGTTGCGCCAGGTACGGGTAATGGCAGGGGCGAATGCCCCTGCCTGGTGTATCTAAGGGTTACAACTCATTCAGTATGTACTGGAACCGGCAAGTAGAATCGATTGGCTGCATACTGCTGGTATCAACACGCACGACCATATCGTGTAACTGTTTGTCCGTTATAGGGTGTCGATTTGGCGTGACCGAGACTTC  >  NZ_CP025268/2453126‑2453397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`