BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,499,085	T→C	81 (TAA→TAG)	yfdY ←	YfdY family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,499,085	0	T	C	99.6%	771.4 / NA	230	81 (TAA→TAG)	yfdY	YfdY family protein
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (133/95); total (135/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 230 total aligned reads displayed.

AAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGAT  >  NZ_CP025268/2498950‑2499219

aagaaATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTAcc                                                                                                                                    <  1:2902411/140‑1 (MQ=255)

 agaaATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCg                                                                                                                                   <  1:4537528/140‑1 (MQ=255)

 agaaATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCg                                                                                                                                   <  2:2653119/140‑1 (MQ=255)

  gaaATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGc                                                                                                                                  <  2:4940327/140‑1 (MQ=255)

  gaaATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGc                                                                                                                                  <  2:1682685/140‑1 (MQ=255)

  gaaATGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGc                                                                                                                                  <  1:2201113/140‑1 (MQ=255)

   aaaTGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGcc                                                                                                                                 <  1:276209/140‑1 (MQ=255)

   aaaTGCCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGcc                                                                                                                                 >  1:2661190/1‑140 (MQ=255)

     aTGCCGCCCCCTTTTCCGACAAAGGCGAAGTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTg                                                                                                                               <  2:3583907/140‑1 (MQ=255)

        ccgccCCCTTTTCCGACGAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCa                                                                                                                            <  2:3191286/140‑1 (MQ=255)

        ccgccCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCa                                                                                                                            <  2:147799/140‑1 (MQ=255)

          gccCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATa                                                                                                                          >  2:673122/1‑140 (MQ=255)

          gccCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATa                                                                                                                          <  1:3525626/140‑1 (MQ=255)

          gccCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATa                                                                                                                          >  1:2353231/1‑140 (MQ=255)

          gccCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATa                                                                                                                          >  1:1601062/1‑140 (MQ=255)

             cccTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATAccc                                                                                                                       <  1:3848190/140‑1 (MQ=255)

                    ccGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATa                                                                                                                <  2:1810381/140‑1 (MQ=255)

                    ccGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATa                                                                                                                <  1:3265836/140‑1 (MQ=255)

                    ccGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGACGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATa                                                                                                                <  2:3685777/140‑1 (MQ=255)

                       aCAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAg                                                                                                             >  1:928467/1‑140 (MQ=255)

                          aaGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGa                                                                                                          <  2:4343307/140‑1 (MQ=255)

                           aGGCGAATTATCTTCTTCATCCTGTCCCATAGCCCCTTTCACCCGGCAGGTGCAGAATTATGACTGATGCTGATGTGAACACCTGCCCAAATGACCGATCCTGACCCACTACTGACTGCCATGCCCCAGCACAAAGCGaa                                                                                                         >  2:3802938/1‑140 (MQ=255)

                                aaTTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACATTCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAatcatc                                                                                                    >  1:3074711/1‑140 (MQ=255)

                                      cTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCca                                                                                              <  1:1825658/140‑1 (MQ=255)

                                          ttCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAg                                                                                          >  2:4695169/1‑140 (MQ=255)

                                          ttCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAg                                                                                          >  1:4254891/1‑140 (MQ=255)

                                           tCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGc                                                                                         <  1:4550170/140‑1 (MQ=255)

                                               tcTGTGCGATAGCGTCTTTCACATGGCAGGTGATCAATCCTGTGTTATGATGATGTGAAATCTTTTCTATATGAGCTTTTCTGACCCATGACCGCTTGTCATACCCCATCTTAAGGCGAATCATCAAGCCTCAAGCCgtg                                                                                     >  2:4471804/2‑138 (MQ=255)

                                               ccTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAATCCACAAGCCggg                                                                                     >  2:2757692/1‑140 (MQ=255)

                                               ccTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCggg                                                                                     >  2:3697045/1‑140 (MQ=255)

                                               ccTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCggg                                                                                     >  2:622688/1‑140 (MQ=255)

                                               ccTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCggg                                                                                     >  1:4870519/1‑140 (MQ=255)

                                                   tgCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCaa                                                                                 >  2:519862/1‑140 (MQ=255)

                                                   tgCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCaa                                                                                 >  2:1229323/1‑140 (MQ=255)

                                                   tgCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCaa                                                                                 >  1:579304/1‑140 (MQ=255)

                                                   tgCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCaa                                                                                 >  1:4122002/1‑140 (MQ=255)

                                                   tgCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCaa                                                                                 >  1:1724443/1‑140 (MQ=255)

                                                   tgCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCaa                                                                                 >  2:58821/1‑140 (MQ=255)

                                                   tgCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCaa                                                                                 >  1:1930059/1‑140 (MQ=255)

                                                   tgCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCC‑AATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCa                                                                                  <  2:3805873/138‑1 (MQ=255)

                                                   tgCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCC‑AATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCa                                                                                  >  1:3805873/1‑138 (MQ=255)

                                                   tgCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCGGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCACCCAGCCGGGGCaa                                                                                 >  2:2723218/1‑140 (MQ=255)

                                                   tgCGATAGCGTCTTTCACATGGCAGGTGAACAATCAGGTCGGAGGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTCCCATACCCCACCATAAAGCGAAACATCAAGCCACAAGCCGGGGCaa                                                                                 >  2:4912318/1‑140 (MQ=255)

                                                    gCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAg                                                                                <  2:254262/140‑1 (MQ=255)

                                                    gCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAg                                                                                <  2:4127623/140‑1 (MQ=255)

                                                     cGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGa                                                                               <  2:1465343/140‑1 (MQ=255)

                                                         agcGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACa                                                                           >  1:3494468/3‑140 (MQ=255)

                                                             tCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACAGCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATaaa                                                                       >  2:2837199/1‑140 (MQ=255)

                                                             tCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATaaa                                                                       >  2:2860047/1‑140 (MQ=255)

                                                             tCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATaaa                                                                       >  1:2027094/1‑140 (MQ=255)

                                                               tttCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAata                                                                     <  1:3502376/140‑1 (MQ=255)

                                                               tttCACATGGCAGGTGAACAATCATGTCTGATGCTGATGAGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCGGCCATACCCCACCATAAAGCGAATAATCAAGCCACAAGCCGGGGCAAGAAACATAAata                                                                     >  1:4072285/1‑140 (MQ=255)

                                                                  cacaTGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATcc                                                                  >  1:3140973/1‑140 (MQ=255)

                                                                   acaTGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATccc                                                                 <  2:4169626/140‑1 (MQ=255)

                                                                    caTGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATcccc                                                                <  1:2744509/140‑1 (MQ=255)

                                                                      tGGCAGGTGAACCATCATGTCTGATTATGATGTGAACACCTGCCAAAATCAGCCATCCCGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGt                                                              <  2:4620233/140‑1 (MQ=255)

                                                                         cAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGTCAAGAAACATAAATAGCCCCGTCAc                                                           >  2:2677839/1‑140 (MQ=255)

                                                                             tGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGt                                                       >  2:710886/1‑140 (MQ=255)

                                                                             tGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGt                                                       <  1:2303288/140‑1 (MQ=255)

                                                                                aaaaTCTTGTCTAATGCTGATGTGTTCCCCTTCCCAAATGAGACATCCTGTCCCACTGCCGCCTTTTATACCCCACCATAAATCTAATCTTCTAGCCACAAGCCGGGGCAAGAAACATAAAGATCCCCGTCACTAGTTCa                                                    <  2:4980763/138‑1 (MQ=255)

                                                                                 cAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCAt                                                   >  1:1696286/1‑140 (MQ=255)

                                                                                      aTGTCTGATGCTGATGTGAACACCTGCCTAAATGAGCCATCCTGACGCAGTACCGCCGGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACCAGTTCATTACCg                                              >  2:2687694/1‑140 (MQ=255)

                                                                                       tGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGc                                             <  1:2914279/140‑1 (MQ=255)

                                                                                         tCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATAccc                                                                                                                       >  2:3960869/1‑64 (MQ=255)

                                                                                         tCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCa                                           >  2:5077514/1‑140 (MQ=255)

                                                                                           tgatgctgatgTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGt                                         >  1:1168739/1‑140 (MQ=255)

                                                                                           tgatgctgatgTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGt                                         <  1:533774/140‑1 (MQ=255)

                                                                                           tgatgctgatgTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGt                                         <  1:1428510/140‑1 (MQ=255)

                                                                                            gatgctgatgTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGtt                                        >  1:3111499/1‑140 (MQ=255)

                                                                                              tgctgatgTGAACACCTGCCCAAATGATCCATCCTGACCCACTACCGCGTGCCATACCCCACCATAAAGCGAATCATCAAGCCAGAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTAACGCCAGTTaa                                      >  2:3739331/1‑140 (MQ=255)

                                                                                              tgctgatgTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTaa                                      >  1:759485/1‑140 (MQ=255)

                                                                                              tgctgatgTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTaa                                      >  1:1786041/1‑140 (MQ=255)

                                                                                              tgctgatgTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTaa                                      >  2:3329362/1‑140 (MQ=255)

                                                                                              tgctgatgTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTaa                                      >  1:4407614/1‑140 (MQ=255)

                                                                                              tgctgatgTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTaa                                      >  1:2256748/1‑140 (MQ=255)

                                                                                                ctgatgTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAAcc                                    <  1:1966846/140‑1 (MQ=255)

                                                                                                   atgTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACcaca                                 >  2:4313378/1‑140 (MQ=255)

                                                                                                   atgTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACcaca                                 >  1:3605372/1‑140 (MQ=255)

                                                                                                   atgTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAATTCATTACCGCCAGTTAACcaca                                 <  1:3067601/140‑1 (MQ=255)

                                                                                                       gAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGt                             <  2:1980017/140‑1 (MQ=255)

                                                                                                        aaCACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTg                            >  2:403896/1‑140 (MQ=255)

                                                                                                        aaCACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGGTAACCACATGGTg                            >  2:4396123/1‑140 (MQ=255)

                                                                                                         ccacCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCCAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGa                           <  1:1557771/139‑1 (MQ=255)

                                                                                                         acacCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCACGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGa                           <  2:4511707/140‑1 (MQ=255)

                                                                                                         acacCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGa                           <  2:3110082/140‑1 (MQ=255)

                                                                                                         acacCTGCCCAAATGAGCAATTCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGa                           <  1:2553545/140‑1 (MQ=255)

                                                                                                         acacCCGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGa                           <  2:1892574/140‑1 (MQ=255)

                                                                                                             cTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAgga                       >  1:1033199/1‑138 (MQ=255)

                                                                                                             cTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAg                         >  2:220376/1‑138 (MQ=255)

                                                                                                                cccAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACGATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGtaata                    >  2:2326957/1‑140 (MQ=255)

                                                                                                                cccAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGtaata                    >  1:975390/1‑140 (MQ=255)

                                                                                                                   aaaTGAGCCATCCTGACCCACTACCGCCTGCTATACCCCACCATAAAGCGAATCATCAGGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAAt                 <  2:3072862/140‑1 (MQ=255)

                                                                                                                     aTGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGACTCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCACGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGa               >  2:4208072/1‑140 (MQ=255)

                                                                                                                      tGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAg              <  1:4797561/140‑1 (MQ=255)

                                                                                                                      tGAGCCATCATGCCCCACTACCGCCTGCCATACCCCACCATAACGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAg              <  2:2723831/140‑1 (MQ=255)

                                                                                                                       gAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCcac                                                                                             >  1:4169547/1‑60 (MQ=255)

                                                                                                                          ccATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCa          >  1:4348233/1‑140 (MQ=255)

                                                                                                                          ccATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCa          >  1:5059570/1‑140 (MQ=255)

                                                                                                                          ccATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCa          >  1:1213828/1‑140 (MQ=255)

                                                                                                                           cATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAg         <  2:2441990/140‑1 (MQ=255)

AAGAAATGCCGCCCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGAT  >  NZ_CP025268/2498950‑2499219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`