BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,625,331	G→A	E37E (GAG→GAA)	purM →	phosphoribosylformylglycinamidine cyclo‑ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,625,331	0	G	A	100.0%	820.2 / NA	218	E37E (GAG→GAA)	purM	phosphoribosylformylglycinamidine cyclo‑ligase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (138/80); total (138/80)

Only 100 of 218 total aligned reads displayed.

AGTAACGCGTGGGGACCCAAGCAGTGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACAC  >  NZ_CP025268/2625198‑2625465

aGTAACGCGTGGGGACCCAAGCAGTGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGAGGCGGGTAATGCTCTGGGTGGAATAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATg                                                                                                                                  >  1:1426406/1‑140 (MQ=255)

 gTAACGCGTGGGGACCCAAGCAGTGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATgg                                                                                                                                 <  2:3236677/140‑1 (MQ=255)

         tGGGGACCCAAGCAGTGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTg                                                                                                                         >  2:3550574/1‑140 (MQ=255)

          ggggACCCAAGCAGTGACCGAAAAAACCTCTGTTAGGTACCTAGATGCCGGTGGTGATATTGCCGCGGGTGAGGCTCTGGTCGGAAGAATCAAACGTGTAGTGAGTAAAACGCGTGGGCCGGAAGGGATGGGCGGtgtcg                                                                                                                        >  2:2691896/1‑136 (MQ=255)

           gggACCCAAGCAGTGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTggg                                                                                                                       >  2:1843860/1‑140 (MQ=255)

                ccAAGCAGTGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCt                                                                                                                  >  2:1524241/1‑140 (MQ=255)

                ccAAGCAGTGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCt                                                                                                                  <  1:3299465/140‑1 (MQ=255)

                ccAAGCAGTGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCt                                                                                                                  <  1:1921006/140‑1 (MQ=255)

                  aaGCAGTGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTc                                                                                                                >  1:1103311/1‑140 (MQ=255)

                   aGCAGTGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCg                                                                                                               <  1:40465/140‑1 (MQ=255)

                   aGCAGTGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCg                                                                                                               <  1:3171808/140‑1 (MQ=255)

                     cAGTGACCGATCCAACCTCTCTTGAATACAAACATGCCGGTGTTGCTATTGACGCGAGTAATGCTCTGGTTGGAAGAACCAAAGGCGTAGTGAAGCAAACGCGTCGTCCGGAAGTCATGGGCGGTCTGGGCGGCTTCGGt                                                                                                             <  2:1490446/140‑1 (MQ=255)

                     cAGTGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGt                                                                                                             <  1:1719467/140‑1 (MQ=255)

                      aGTGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTg                                                                                                            <  1:4395886/140‑1 (MQ=255)

                      aGTGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTg                                                                                                            <  2:2065409/140‑1 (MQ=255)

                        tGACTGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATAGTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTgcg                                                                                                          <  2:4336374/140‑1 (MQ=255)

                        tGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTgcg                                                                                                          <  2:2648905/140‑1 (MQ=255)

                        tGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTgcg                                                                                                          <  2:2122901/140‑1 (MQ=255)

                        tGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTgcg                                                                                                          <  1:153161/140‑1 (MQ=255)

                        tGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTgcg                                                                                                          >  1:3567506/1‑140 (MQ=255)

                        tGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGGAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTgcg                                                                                                          >  2:2659670/1‑140 (MQ=255)

                        tGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAACAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTgcg                                                                                                          <  2:4552959/140‑1 (MQ=255)

                         gACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTgcgc                                                                                                         <  1:4096264/140‑1 (MQ=255)

                          aCCGATACAACCTCTCTTAGCTCCAAAGATGCCGGTGTTGATATTGACGCGGGTCATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGACGACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCt                                                                                                        <  2:4010559/140‑1 (MQ=255)

                          aCCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCt                                                                                                        >  1:1204031/1‑140 (MQ=255)

                          aCCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCt                                                                                                        >  2:3599351/1‑140 (MQ=255)

                          aCCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCt                                                                                                        >  1:495247/1‑140 (MQ=255)

                              aTAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCtgtgt                                                                                                    >  1:2365219/1‑140 (MQ=255)

                               tAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCtgtgtg                                                                                                   >  1:896478/1‑140 (MQ=255)

                                  aaCCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCAt                                                                                                >  2:84160/1‑140 (MQ=255)

                                  aaCCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGAAGGGCGTTCTCGGCGGCTGCTGTGCGCTGGGTGCAt                                                                                                >  1:635853/1‑140 (MQ=255)

                                    ccTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTg                                                                                              >  2:3926194/1‑140 (MQ=255)

                                      tctctTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGcc                                                                                            >  2:412361/1‑140 (MQ=255)

                                        tctTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGc                                                                                          >  2:699950/1‑140 (MQ=255)

                                            aGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCaaca                                                                                      >  1:1149316/1‑138 (MQ=255)

                                             gCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCaaaaa                                                                                     <  2:1513108/140‑1 (MQ=255)

                                                aCAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAatat                                                                                  >  1:3131213/1‑140 (MQ=255)

                                                  aaaGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCg                                                                                >  1:3528624/1‑140 (MQ=255)

                                                  aaaGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCg                                                                                >  2:1386219/1‑140 (MQ=255)

                                                  aaaGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCg                                                                                >  2:465720/1‑140 (MQ=255)

                                                    aGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGGGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTg                                                                              >  1:94620/1‑140 (MQ=255)

                                                       tGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGGCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAAc                                                                           >  2:659865/1‑140 (MQ=255)

                                                                tGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACGCGTGCTgg                                                                  >  1:744158/1‑140 (MQ=255)

                                                                ggATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTGGTGAAGGAGACGCGTCGTCCGGAAGTGGTGGGCGGTCTGGGGGGCTGCGGTGCGGTGTGGGCATGGTCGCAGAAGTGTGGTGAACCCGTGCggg                                                                  >  2:1974473/2‑140 (MQ=255)

                                                                  atatTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGAAAAAATATCGTGAACCCGTGCTGGtt                                                                >  2:1254820/1‑140 (MQ=255)

                                                                    atTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTc                                                              >  1:1178720/1‑140 (MQ=255)

                                                                       gACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTgg                                                           <  1:857776/140‑1 (MQ=255)

                                                                         cgcgGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCa                                                         >  2:1889459/1‑140 (MQ=255)

                                                                           cgGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACt                                                       <  1:210289/140‑1 (MQ=255)

                                                                            gggTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTg                                                      <  1:2182200/140‑1 (MQ=255)

                                                                             ggTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGa                                                     >  2:4205578/1‑140 (MQ=255)

                                                                             ggTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGa                                                     >  2:3901476/1‑140 (MQ=255)

                                                                              gTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGAc                                                    <  2:3131213/140‑1 (MQ=255)

                                                                               tAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACg                                                   <  1:1880880/140‑1 (MQ=255)

                                                                                    ctctGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTa                                              >  1:2050597/1‑140 (MQ=255)

                                                                                    ctctGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGGCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTa                                              >  1:1176487/1‑140 (MQ=255)

                                                                                      ctGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGGGGTCTGGGATGCTTCTGTGCGCTATGTGCATCTCAGGATAATTATCGTGAACCGGCGCTGGTCTCTGGCACTGACCGCGTAgg                                            >  2:1560203/1‑140 (MQ=255)

                                                                                       tGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGt                                           >  1:1828978/1‑140 (MQ=255)

                                                                                       tGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGt                                           >  1:2082275/1‑140 (MQ=255)

                                                                                       tGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGt                                           >  2:5119408/1‑140 (MQ=255)

                                                                                        ggTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTa                                          <  2:4945846/140‑1 (MQ=255)

                                                                                        ggTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTa                                          >  2:2230732/1‑140 (MQ=255)

                                                                                        ggTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTa                                          >  2:3293862/1‑140 (MQ=255)

                                                                                           tGGAAGCATCAAAGGCGTAGTGAAGAAAACGCGTCGGCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCa                                       <  1:1677971/140‑1 (MQ=255)

                                                                                            ggAAGAATCAAAGGCATAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCaa                                      >  2:317686/1‑140 (MQ=255)

                                                                                              aagaaTCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGc                                    >  1:3987498/1‑140 (MQ=255)

                                                                                               agaaTCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCt                                   >  1:3818233/1‑140 (MQ=255)

                                                                                                   tCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGt                               >  2:3380951/1‑140 (MQ=255)

                                                                                                   tCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGt                               >  1:4613855/1‑140 (MQ=255)

                                                                                                   tCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGt                               >  2:126627/1‑140 (MQ=255)

                                                                                                   tCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGt                               >  2:1574995/1‑140 (MQ=255)

                                                                                                   tCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGGGCTGGTATCTGGCACTGCCGGCGTAGGTACCAAGCTGCGt                               >  2:2824379/1‑140 (MQ=255)

                                                                                                    cAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTc                              <  2:2367453/140‑1 (MQ=255)

                                                                                                    cAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTc                              >  2:1596916/1‑140 (MQ=255)

                                                                                                    cAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTc                              <  2:3990411/140‑1 (MQ=255)

                                                                                                         gCGTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCa                         <  1:3763820/140‑1 (MQ=255)

                                                                                                           gTAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAAt                       <  1:2465304/140‑1 (MQ=255)

                                                                                                            tAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATg                      >  1:2619103/1‑140 (MQ=255)

                                                                                                            tAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATg                      >  2:249806/1‑140 (MQ=255)

                                                                                                            tAGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATg                      >  2:1694321/1‑140 (MQ=255)

                                                                                                            tAGCGAATAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGATCTGTGGGCGTTGCGGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACGAAGCTGCGTCTGGCAATg                      >  1:4011914/1‑140 (MQ=255)

                                                                                                             aGTGAAGAAAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATgg                     >  1:1290068/1‑140 (MQ=255)

                                                                                                                   gaaAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAg                                     >  2:4643631/1‑118 (MQ=255)

                                                                                                                   gaaAACGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAg                                     <  1:4643799/118‑1 (MQ=255)

                                                                                                                    aaaaCGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTaaa              >  1:2724809/1‑140 (MQ=255)

                                                                                                                     aaaCGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGCCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGCGACCCCGTGCTCGTTTCTGGCACCGACGGCGTATGTACCAAGCTGCGTCTGGCAATGGACTTaaaa             <  2:430831/140‑1 (MQ=255)

                                                                                                                      aaCGCGTCGTCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGGAGGTACCAAGCTGCGTCTGGCAATGGACTTaaaaa            >  2:2865687/1‑139 (MQ=255)

                                                                                                                          cgtcgtCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGTGCTGTGTGCATTGCCCAAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGCTCCCAAGCTGCGTCTGGCAATGGACTTAAAACGTCa        <  2:1043412/140‑1 (MQ=255)

                                                                                                                          cgtcgtCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCa        <  2:4529428/140‑1 (MQ=255)

                                                                                                                          cgtcgtCCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCa        <  2:2725363/140‑1 (MQ=255)

                                                                                                                           gtcgtcCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCAc       >  2:4103932/1‑140 (MQ=255)

                                                                                                                           gtcgtcCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCAc       >  1:2940207/1‑140 (MQ=255)

                                                                                                                           gtcgtcCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGGGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCAc       >  2:4391147/1‑140 (MQ=255)

                                                                                                                             cgtcCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGa     <  1:1386219/140‑1 (MQ=255)

                                                                                                                             cgtcCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGa     <  2:4831955/140‑1 (MQ=255)

                                                                                                                             cgtcCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGa     <  1:4765149/140‑1 (MQ=255)

                                                                                                                              gtcCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGac    <  1:4865022/140‑1 (MQ=255)

                                                                                                                              gtcCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGac    <  1:4349974/140‑1 (MQ=255)

                                                                                                                              gtcCGGAAGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGac    <  2:879868/140‑1 (MQ=255)

                                                                                                                              gtcCGGAAGTGATGGGCGGTCTGGGCGGCTTCAGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGac    <  2:3839735/140‑1 (MQ=255)

AGTAACGCGTGGGGACCCAAGCAGTGACCGATAAAACCTCTCTTAGCTACAAAGATGCCGGTGTTGATATTGACGCGGGTAATGCTCTGGTTGGAAGAATCAAAGGCGTAGTGAAGAAAACGCGTCGTCCGGAGGTGATGGGCGGTCTGGGCGGCTTCGGTGCGCTGTGTGCATTGCCGCAAAAATATCGTGAACCCGTGCTGGTTTCTGGCACTGACGGCGTAGGTACCAAGCTGCGTCTGGCAATGGACTTAAAACGTCACGACAC  >  NZ_CP025268/2625198‑2625465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`