BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,654,514	T→C	M600V (ATG→GTG)	yfhM ←	alpha2‑macroglobulin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,654,514	0	T	C	99.7%	1049.9 / NA	313	M600V (ATG→GTG)	yfhM	alpha2‑macroglobulin
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (168/143); minor base A (0/1); total (169/144)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 313 total aligned reads displayed.

ACTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCATCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCATCCAG  >  NZ_CP025268/2654380‑2654646

 ctgcctgcATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATTATCGTAACCCGATTACGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCGCAGCGCGGCGCGGCACCCGGCg                                                                                                                                >  2:3095762/1‑140 (MQ=255)

      ttCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGc                                                                                                                           >  1:1898182/1‑140 (MQ=255)

       tcatcaACAATGGGCTGTTTCACCGTGCTGTAAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGcc                                                                                                                          <  2:3417256/140‑1 (MQ=255)

        catcaACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGccc                                                                                                                         >  1:4920280/1‑140 (MQ=255)

        catcaACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGccc                                                                                                                         >  1:982790/1‑140 (MQ=255)

         aagaacaaTTGGCTGTTTAACCGTGCTGTCAGTACGATAACCGTAAACCGATTGCGGTGCGAACTGCGGATGGATCGCCGGCAATGCATCGGCAGGCCAGATAGCGTGCTCAGCGCGGCTCGTCAGGGGGAGACCGGCCg                                                                                                                        >  2:3047871/4‑140 (MQ=255)

              caaTGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTcc                                                                                                                   >  1:3502604/1‑140 (MQ=255)

                aTGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAg                                                                                                                 >  1:3755368/1‑140 (MQ=255)

                 tGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGc                                                                                                                >  2:3332996/1‑140 (MQ=255)

                   ggCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCaa                                                                                                              >  2:1616649/1‑140 (MQ=255)

                   ggCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCaa                                                                                                              >  1:2097763/1‑140 (MQ=255)

                      tGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACt                                                                                                           >  1:2738777/1‑140 (MQ=255)

                       gTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTa                                                                                                          <  1:4446290/140‑1 (MQ=255)

                        tttCACCGTGCTGTCCGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCCATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACtcc                                                                                                         >  1:3996763/1‑138 (MQ=255)

                        tttCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTAc                                                                                                         >  1:1258255/1‑140 (MQ=255)

                        tttCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTAc                                                                                                         >  2:2963238/1‑140 (MQ=255)

                        tttCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTAc                                                                                                         >  2:2519710/1‑140 (MQ=255)

                        tttCACCGTGCTGCCAGTACGATAATCGTAAACCGTTTTCGAGGCGAACTGCGGACGGATCTCCGGCCATTCACCGGCCGGCCAGACAGCCTGCTCACCGCGGCCCGTCACCGGGCGACCGCCCCATTCCACCCAACTAc                                                                                                         >  2:1029420/1‑140 (MQ=255)

                         ttCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCTGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTAcc                                                                                                        <  1:4704914/140‑1 (MQ=255)

                          tCACCGTGGTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGATCTGCGGGCGGTTCCCCGGCAATGCATCGGTAGGCCAGTTAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTAccc                                                                                                       <  2:3810379/140‑1 (MQ=255)

                          tCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGGCACCGGGCGACCGCCCGATTCCAGCAAACTAccc                                                                                                       <  1:1850519/140‑1 (MQ=255)

                           cACTGTGCTGTCAGTACGATAATCGTAACCCGATTTCGAGGCGAACTGCGGACGGTTCCCCGGCAATGCATCGGCTGGCCAGATAGCAGGCTCAGCGCGGCGCGTCTCCGGGCGACCGCCCGATTCCAGCCAACTACCCt                                                                                                      <  2:3659096/140‑1 (MQ=255)

                           cACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCt                                                                                                      <  1:916359/140‑1 (MQ=255)

                             ccGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTgg                                                                                                    >  2:4260258/1‑140 (MQ=255)

                                  cTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAACAt                                                                                               >  1:4871723/1‑140 (MQ=255)

                                  cTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAAt                                                                                               <  1:3929245/140‑1 (MQ=255)

                                  cTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAAt                                                                                               >  2:467596/1‑140 (MQ=255)

                                   tGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATa                                                                                              <  2:2750359/140‑1 (MQ=255)

                                     tCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGCAAATAAc                                                                                            <  2:4047221/140‑1 (MQ=255)

                                     tCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAAc                                                                                            <  2:3953908/140‑1 (MQ=255)

                                     tCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAAc                                                                                            <  1:3295817/140‑1 (MQ=255)

                                     tCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAAc                                                                                            <  1:1151878/140‑1 (MQ=255)

                                     tCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGAGCCTCGGCAATGCATCGGCAGCCCAGATAGCCTGCTCAGCGCTGCGCGTCACCGGGCGACCGCACGATTCCAGCAAACTACCCTGGAAAATAAc                                                                                            <  1:3051334/140‑1 (MQ=255)

                                     tCAGTACCATAATCGTAATCCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAAc                                                                                            <  2:4889190/140‑1 (MQ=255)

                                       aGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCt                                                                                          >  2:1876529/1‑140 (MQ=255)

                                          aCGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCGGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTa                                                                                       >  2:3530971/1‑140 (MQ=255)

                                          aCGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTa                                                                                       <  1:1440920/140‑1 (MQ=255)

                                            gATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAAt                                                                                     >  1:3316847/1‑140 (MQ=255)

                                               aaTCGTATACCGACTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATAGGCAGGCCAGATAGGCTGCTCGGCGCGGCGCGTCACCGGGCGACCGCCCGATCCCAGCAAACTACCCTGGAAAATAACCTGTAATggg                                                                                  >  2:1370740/1‑140 (MQ=255)

                                               aaTCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATggg                                                                                  >  2:1725641/1‑140 (MQ=255)

                                                   gTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTGACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAAt                                                                              >  1:4254621/1‑140 (MQ=255)

                                                   gTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAAt                                                                              >  1:2903556/1‑140 (MQ=255)

                                                   gTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAAt                                                                              >  1:231493/1‑140 (MQ=255)

                                                   gTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAAt                                                                              >  1:550176/1‑140 (MQ=255)

                                                   gTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAACGGGGAAt                                                                              <  1:293789/140‑1 (MQ=255)

                                                      acccGATTACGAGTTTAACAGCGGTCTGATCCCCCACAATGCATCCACACTCGAGACATCCTGCGCAGCGCGGCGCGTCACCAGGCGACCGCCCGATCCCAGCAAACATCCCTGGAAAATAACCTGTAATGGGGCATCCg                                                                           <  2:3904547/138‑1 (MQ=255)

                                                      aaCCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGct                                                                           >  1:4500409/1‑139 (MQ=255)

                                                      aaCCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCg                                                                           >  1:1004002/1‑140 (MQ=255)

                                                      aaCCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCg                                                                           <  2:3882984/140‑1 (MQ=255)

                                                           aTTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCTAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTttcc                                                                      >  2:4002029/1‑140 (MQ=255)

                                                           aTTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTttcc                                                                      >  1:3940864/1‑140 (MQ=255)

                                                            tttCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTttcct                                                                     >  1:4777093/1‑140 (MQ=255)

                                                                 gtgCGAACTGCGGACGGATCCCCGTCAAGGCATCTGCAGGCCAGATAGCCTGCTCATCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAATCTACCCTGGAAAATAACCTGTATTGGGGAATGCGTTTCCTTCCAc                                                                <  2:1965093/138‑1 (MQ=255)

                                                                 aGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCAc                                                                <  2:2318286/140‑1 (MQ=255)

                                                                 aGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCAc                                                                <  1:2607117/140‑1 (MQ=255)

                                                                      aaCTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCt                                                           >  2:1452927/1‑140 (MQ=255)

                                                                       aCTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCtt                                                          <  2:2865919/140‑1 (MQ=255)

                                                                       aCTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCtt                                                          <  1:64060/140‑1 (MQ=255)

                                                                       aCTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCtt                                                          <  2:1863916/140‑1 (MQ=255)

                                                                        cTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCttt                                                         <  1:4979165/140‑1 (MQ=255)

                                                                         tGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTc                                                        <  2:4785003/140‑1 (MQ=255)

                                                                             gacggaTCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTa                                                    >  1:3413695/1‑140 (MQ=255)

                                                                                  aTCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAAc                                               >  1:1040747/1‑140 (MQ=255)

                                                                                   tCCCCGGCAATGCATCGGCAGGCCAGATAGCTTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATGCCAGCAACCTCCCCTGCAAAATAACCTGCAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGCAACt                                              >  2:4163547/1‑140 (MQ=255)

                                                                                   tCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACt                                              >  2:1170177/1‑140 (MQ=255)

                                                                                   tCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACt                                              <  1:3003965/140‑1 (MQ=255)

                                                                                   tCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCGTTCTGTAGAAACt                                              >  2:4962250/1‑140 (MQ=255)

                                                                                        ggCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTcgcc                                         >  2:3216835/1‑140 (MQ=255)

                                                                                        ggCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTcgcc                                         <  1:1069806/140‑1 (MQ=255)

                                                                                        ggCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCGGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCGTTCTGTAGAAACTTagcc                                         <  2:638687/140‑1 (MQ=255)

                                                                                         gCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTcgccg                                        <  2:2304091/140‑1 (MQ=255)

                                                                                         gCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTcgccg                                        <  1:2238400/140‑1 (MQ=255)

                                                                                         gCAAGGCAGCGGCAGGCCAGATAGCCTGCTCACCGCGGCGCGTCCACGGGCGACCGCCCGATTCCAGCCAACGACCCTGCAAAATAACCTGTAAGGGGGAACGCGTTTCCTTCCACTGGCTTGCTGTAGAAACTCctccg                                        <  2:2578339/140‑1 (MQ=255)

                                                                                           aaTGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCcgcg                                      <  1:3670329/140‑1 (MQ=255)

                                                                                           aaTGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCcgcg                                      <  1:1775566/140‑1 (MQ=255)

                                                                                           aaTGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCcgcg                                      <  2:4350205/140‑1 (MQ=255)

                                                                                           aaTGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCcgcg                                      >  1:2536912/1‑140 (MQ=255)

                                                                                            aTGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCgcgc                                     >  1:4561545/1‑140 (MQ=255)

                                                                                            aTGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCgcgc                                     >  1:1918079/1‑140 (MQ=255)

                                                                                             tGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCTGGCGCCCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGcc                                    <  2:2343893/140‑1 (MQ=255)

                                                                                             tGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGcc                                    >  2:2928835/1‑140 (MQ=255)

                                                                                             tGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGcc                                    <  2:3260884/140‑1 (MQ=255)

                                                                                             tGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGcc                                    <  2:285672/140‑1 (MQ=255)

                                                                                             tGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGcc                                    >  1:137776/1‑140 (MQ=255)

                                                                                             tGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGcc                                    <  1:816780/140‑1 (MQ=255)

                                                                                              gCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGccc                                   >  1:4065212/1‑140 (MQ=255)

                                                                                                  cGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTa                               <  1:1647982/140‑1 (MQ=255)

                                                                                                        gCCCGATAGCCTGCTCAGCGCGGCGCGTCACCGAGGGACCGCCCGATTACAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATcc                         <  2:760808/140‑1 (MQ=255)

                                                                                                             aTAGCCTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGt                    >  1:5080413/1‑140 (MQ=255)

                                                                                                                  cTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACt               <  2:2104322/140‑1 (MQ=255)

                                                                                                                  cTGCTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGCCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACt               >  1:4289477/1‑140 (MQ=255)

                                                                                                                    gCTCAGCGCGGCGCGTCACCGGGCGACCGCCCTATTCCAGCAAACTACCCGGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGa             >  1:381868/1‑140 (MQ=255)

                                                                                                                     cTCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGaa            >  1:77775/1‑140 (MQ=255)

                                                                                                                      tCAGCGCGGCGCGTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAAc           <  1:1225983/140‑1 (MQ=255)

                                                                                                                          cgcggcgcgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCa       >  1:2035016/1‑140 (MQ=255)

                                                                                                                          cgcggcgcgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCa       >  1:323523/1‑140 (MQ=255)

                                                                                                                          cgcggcgcgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCa       >  1:4135372/1‑140 (MQ=255)

                                                                                                                          cgcggcgcgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCa       >  2:2606282/1‑140 (MQ=255)

                                                                                                                          cgcggcgcgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCCTCAACTGAACTTCa       >  1:505060/1‑140 (MQ=255)

                                                                                                                               cgcgTCACCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCATCCAg  >  1:1591920/1‑140 (MQ=255)

ACTGCCTTCATCAACAATGGGCTGTTTCACCGTGCTGTCAGTACGATAATCGTAAACCGATTTCGAGGCGAACTGCGGACGGATCCCCGGCAATGCATCGGCAGGCCAGATAGCCTGCTCAGCGCGGCGCGTCATCGGGCGACCGCCCGATTCCAGCAAACTACCCTGGAAAATAACCTGTAATGGGGAATGCGTTTCCTTCCACTGGCTTTCTGTAGAAACTTCGCCGCGCCCTTTATCATCCAGCGTCAACTGAACTTCATCCAG  >  NZ_CP025268/2654380‑2654646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`