BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,939,236	C→T	L450F (CTC→TTC)	fucI →	L‑fucose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,939,236	0	C	T	99.7%	1223.0 / NA	335	L450F (CTC→TTC)	fucI	L‑fucose isomerase
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (116/218); total (117/218)
Fisher's exact test for biased strand distribution p-value = 3.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 351 total aligned reads displayed.

ACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCA  >  NZ_CP025268/2939100‑2939371

 cgcgACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGcttcc‑t                                                                                                                                     >  2:1045404/1‑140 (MQ=255)

 cgcgACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGcttcc‑t                                                                                                                                     >  1:5099319/1‑140 (MQ=255)

 cgcgACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGcttcc‑t                                                                                                                                     >  1:2646867/1‑140 (MQ=255)

 cgcgACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGcttcc‑t                                                                                                                                     >  1:2413658/1‑140 (MQ=255)

 cgcgACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGcttcc‑t                                                                                                                                     >  1:1563795/1‑140 (MQ=255)

 cgcgACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGcttcc‑t                                                                                                                                     >  1:1729706/1‑140 (MQ=255)

 cacgACAGCGCAGGCAACCCGCCGTTGAAACCACACTAGGAAATCTCTCAGCCAGAGGCTGACGCTTGCCTCGCCGCTACCTAATGGTGCACGACGATCAACGAATACTTCCGTGCCGCCGGGTACTCTTCCCGcttcc‑t                                                                                                                                     >  2:664875/3‑140 (MQ=255)

    gACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTAc                                                                                                                                  >  2:2563354/1‑140 (MQ=255)

         cGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAg                                                                                                                             >  1:2485907/1‑140 (MQ=255)

            aGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTAC‑TTACCGAAggcg                                                                                                                          <  2:264102/140‑1 (MQ=255)

               tAACCCGACGATGACGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGgcggcg                                                                                                                       <  2:3113264/140‑1 (MQ=255)

                   ccGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTccc                                                                                                                   >  1:4220316/1‑140 (MQ=255)

                   ccGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGGccc                                                                                                                   >  1:304206/1‑140 (MQ=255)

                    cgacgaTGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCg                                                                                                                  <  1:4856935/140‑1 (MQ=255)

                    cgacgaTGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCg                                                                                                                  <  2:3357249/140‑1 (MQ=255)

                      ccgaTGACGTCACACTGGGCAATCCCTCAGCAAGAGGCTGACGCTTTCCTCGCCGCTATCGAATGGTGCCCGGCGATCCACTATTACTTCCGTGGCGGCGGCTACTCTCCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGtt                                                                                                                <  2:3894124/139‑1 (MQ=255)

                      acgaTGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGtt                                                                                                                >  2:4976914/1‑140 (MQ=255)

                      acgaTGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGtt                                                                                                                <  1:4796132/140‑1 (MQ=255)

                      acgaTGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGtt                                                                                                                <  1:1957858/140‑1 (MQ=255)

                       cgaTGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTc                                                                                                               >  2:1295835/1‑140 (MQ=255)

                            aaGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCAt                                                                                                          <  1:3321685/140‑1 (MQ=255)

                             aGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTAGCGAAGGCGGCGTCCCGTTCACCATg                                                                                                         >  2:4043765/1‑140 (MQ=255)

                               ccACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACCTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGAc                                                                                                       <  1:2974401/140‑1 (MQ=255)

                                cacaCTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACt                                                                                                      <  1:4095827/140‑1 (MQ=255)

                                cacaCTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACt                                                                                                      <  1:2867758/140‑1 (MQ=255)

                                cacaCTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACt                                                                                                      <  1:3918997/140‑1 (MQ=255)

                                   acTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCgt                                                                                                   >  2:3426840/1‑140 (MQ=255)

                                   acTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCgt                                                                                                   <  1:4543959/140‑1 (MQ=255)

                                       ggAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCa                                                                                               >  1:2303173/1‑140 (MQ=255)

                                       ggAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCa                                                                                               >  1:4490244/1‑140 (MQ=255)

                                         aaaTCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAAc                                                                                             <  2:3383401/140‑1 (MQ=255)

                                         aaaTCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAAc                                                                                             <  1:2077314/140‑1 (MQ=255)

                                             ctctcAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAAcatca                                                                                         >  2:4887975/1‑140 (MQ=255)

                                             ctctcAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAAcatca                                                                                         >  2:1400256/1‑140 (MQ=255)

                                             ctctcAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGATTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAAcatca                                                                                         >  2:4102012/1‑140 (MQ=255)

                                               ctcAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACatcatc                                                                                       >  1:3588472/1‑140 (MQ=255)

                                                tcAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACAtcatca                                                                                      <  1:147290/140‑1 (MQ=255)

                                                tcAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACAtcatca                                                                                      <  2:3509354/140‑1 (MQ=255)

                                                 cAGTAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGCCGAGCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCaa                                                                                     <  2:3560439/140‑1 (MQ=255)

                                                 cAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGACTGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCaa                                                                                     <  2:2646867/140‑1 (MQ=255)

                                                 cAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCaa                                                                                     <  2:4790267/140‑1 (MQ=255)

                                                 cAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCaa                                                                                     <  1:3938155/140‑1 (MQ=255)

                                                 cAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCaa                                                                                     >  1:1699243/1‑140 (MQ=255)

                                                       gagGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCt                                                                               <  2:950612/140‑1 (MQ=255)

                                                       gagGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCt                                                                               <  1:4506837/140‑1 (MQ=255)

                                                        agGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTg                                                                              >  1:1791029/1‑140 (MQ=255)

                                                          gCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTggg                                                                            <  1:613434/140‑1 (MQ=255)

                                                          gCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCAGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTggg                                                                            >  2:71066/1‑140 (MQ=255)

                                                            tgccGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATTCTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGAc                                                                          <  2:1959982/137‑1 (MQ=255)

                                                            tGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCCCGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGAc                                                                          <  2:1506693/140‑1 (MQ=255)

                                                            tGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTCCTCTTCCCGCTTCC‑TTACCGATGGCGGCGTCCCGTTCACCATGTCTCGTGTCAACATCATCAAAGGCCTGGGAc                                                                          <  2:4743413/140‑1 (MQ=255)

                                                            tGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGAc                                                                          <  1:2107620/140‑1 (MQ=255)

                                                            tGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGAc                                                                          <  2:2307007/140‑1 (MQ=255)

                                                            tGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGAc                                                                          <  1:1736940/140‑1 (MQ=255)

                                                            tGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGAc                                                                          <  2:4260947/140‑1 (MQ=255)

                                                            tGACGCTTGCCTCGCCGCCACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TCACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGAc                                                                          <  2:3634265/140‑1 (MQ=255)

                                                            tGACGCTGGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCGTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGAc                                                                          <  2:4207704/140‑1 (MQ=255)

                                                            tGACGCGTGCCTCGCCGCTCCCGAATGGTGCCCGGCGATCCACGAATACTCCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGAc                                                                          <  2:2672441/140‑1 (MQ=255)

                                                               cGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCgg                                                                       <  2:2722738/140‑1 (MQ=255)

                                                                   tGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACt                                                                   >  2:4345629/1‑140 (MQ=255)

                                                                    gCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTg                                                                  <  1:907196/140‑1 (MQ=255)

                                                                    gCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGGACTg                                                                  >  1:1875871/1‑140 (MQ=255)

                                                                        cgccgcTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCaaa                                                              <  1:2201271/140‑1 (MQ=255)

                                                                             cTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATcgcg                                                         <  1:5001646/140‑1 (MQ=255)

                                                                             cTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATcgcg                                                         <  2:5118936/140‑1 (MQ=255)

                                                                             cTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATcgcg                                                         <  2:2608663/140‑1 (MQ=255)

                                                                             cTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATcgcg                                                         <  1:2553687/140‑1 (MQ=255)

                                                                             cTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATcgcg                                                         <  2:3195609/140‑1 (MQ=255)

                                                                             cTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATcgcg                                                         <  2:3001827/140‑1 (MQ=255)

                                                                               aCCGAGTGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGa                                                       <  2:2130713/140‑1 (MQ=255)

                                                                               aCCGAATGGTGCCCGGCGCTCCACGAATACCTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCCTCCCGTTCACCATCACTCGTGTCAACATCATCACAGGCCTGGGACCGGTACTGCAAATCGCGGa                                                       <  2:1709774/140‑1 (MQ=255)

                                                                                    aTGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCt                                                  <  1:621308/140‑1 (MQ=255)

                                                                                     tGGTGCCCGGCGATCCACGAATAGTTCCGTGGCGGCGGTTACCCTTCGCGCTTCC‑TTACCGAGGGCGGCGTCCCGTTCCCCATGGCTCGTGTCAACCTCATCCAAGGCCTGGGACCGGTACTGCCACTGGCGGAAGGCTg                                                 <  2:3266459/140‑1 (MQ=255)

                                                                                     tGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTg                                                 <  1:4384026/140‑1 (MQ=255)

                                                                                        tGCCCGGCAATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TCACCGACGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATAATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTgggg                                              >  2:3058195/1‑138 (MQ=255)

                                                                                         gCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGc                                             <  2:2092609/140‑1 (MQ=255)

                                                                                         gCCCCGCGACCCACAAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGCCTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCACGGAAGGCTGGGGc                                             <  2:3454839/140‑1 (MQ=255)

                                                                                          cccGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTGAT‑CCGTCTCCGTTACCGAAGTCGGCGATCCGTGCACGTTGAGGCTGGAGAACATGGTGGAAGGCCGAGGATCGGTACTGGAATTTGCGTACGGCTGAAgtt                                            >  2:289331/1‑138 (MQ=255)

                                                                                          cccGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCg                                            >  2:3773109/1‑140 (MQ=255)

                                                                                          cccGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCGGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCg                                            >  1:745247/1‑140 (MQ=255)

                                                                                               cgggccACGAATACTTCCGTGGCGGCCGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCCTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGCGGaa                                       <  1:404409/136‑1 (MQ=255)

                                                                                               cGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGaa                                       <  2:3955217/140‑1 (MQ=255)

                                                                                               cGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGaa                                       <  1:1210167/140‑1 (MQ=255)

                                                                                               cGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGaa                                       >  2:1457464/1‑140 (MQ=255)

                                                                                                 aTCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAAtt                                     >  1:1334485/1‑140 (MQ=255)

                                                                                                    cACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGGCCCGTTCACCATGACTCGTGTCAACATCAGCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGcc                                  <  2:4525145/140‑1 (MQ=255)

                                                                                                     ccGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCg                                 <  1:947400/139‑1 (MQ=255)

                                                                                                     actaaTACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCg                                 <  1:529334/137‑1 (MQ=255)

                                                                                                         aTACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAgg                             <  2:1775159/140‑1 (MQ=255)

                                                                                                            cTTCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGAtg                          >  1:2186295/1‑140 (MQ=255)

                                                                                                             ttCCGTGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGAtgt                         >  2:1930017/1‑140 (MQ=255)

                                                                                                                  tGGCGGCGGTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATg                    >  1:3593737/1‑140 (MQ=255)

                                                                                                                       gcggTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATc               >  2:1665119/1‑140 (MQ=255)

                                                                                                                       gcggTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATc               >  1:1265458/1‑140 (MQ=255)

                                                                                                                       gcggTTACTCTTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATc               >  2:541400/1‑140 (MQ=255)

                                                                                                                              ctctTCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTcaaca        <  1:4928700/140‑1 (MQ=255)

                                                                                                                                  tCCCGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACg    >  1:3374988/1‑140 (MQ=255)

                                                                                                                                   cccGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGc   <  1:4179530/140‑1 (MQ=255)

                                                                                                                                    ccGCTTCC‑TTCCCGAAGGCGGCGTCCCGTTCACCATGATTCGTGCCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCa  <  2:2385108/140‑1 (MQ=255)

                                                                                                                                    ccGCTTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCa  >  1:104501/1‑140 (MQ=255)

ACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCC‑TTACCGAAGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCA  >  NZ_CP025268/2939100‑2939371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`