BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,985,593	T→C	279 (TAA→TAG)	kduI ←	5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,985,593	0	T	C	100.0%	866.8 / NA	256	279 (TAA→TAG)	kduI	5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (115/141); total (115/141)

Only 100 of 256 total aligned reads displayed.

TCACAGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGCT  >  NZ_CP025268/2985457‑2985729

   caGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAgcgc                                                                                                                                    >  1:5071404/1‑140 (MQ=255)

   caGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAgcgc                                                                                                                                    >  1:284216/1‑140 (MQ=255)

    aGCCCGCGTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCa                                                                                                                                   <  2:4090879/140‑1 (MQ=255)

      cccGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCaaa                                                                                                                                 >  1:4064949/1‑140 (MQ=255)

        cGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATc                                                                                                                               >  1:1978726/1‑140 (MQ=255)

          cTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCACATCtt                                                                                                                             >  1:3920480/1‑140 (MQ=255)

          cTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCtt                                                                                                                             >  2:1524262/1‑140 (MQ=255)

             gcgcCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTaa                                                                                                                          <  1:4321529/140‑1 (MQ=255)

             gcgcCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTaa                                                                                                                          <  2:2211675/140‑1 (MQ=255)

             gcgcCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTaa                                                                                                                          <  2:3873605/140‑1 (MQ=255)

              cgcccgccCCAACGCCATCCCCTCAACAAGTCCAGTATCACACCCAGTGACGCCCGCAGCTTTAACTTCGAGAGAAAATGCACTCAACATCCTAACACTGCCTTATTCGTTTATGACCCCAACTAGCGCAAATCTTTAAc                                                                                                                         <  2:655437/140‑1 (MQ=255)

                   gCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCca                                                                                                                    <  2:578799/140‑1 (MQ=255)

                    ccccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcac                                                                                                                   <  1:1369088/140‑1 (MQ=255)

                     cccAACGCGATCCCCCGACCCAGTCCATTACCACAACCAGTGACGACCGCACCTTTACCTTCGAGAGAAAATGCACTTAAAAGCATAACAAGACCTTATTCGTTTATGCCCACTACTAGCGCAATTCTTTAACGGCcaca                                                                                                                  <  2:4807202/140‑1 (MQ=255)

                     cccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                  <  1:4616286/140‑1 (MQ=255)

                     cccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                  <  1:3907725/140‑1 (MQ=255)

                     cccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                  <  2:3433594/140‑1 (MQ=255)

                     cccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                  <  1:4839211/140‑1 (MQ=255)

                     cccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                  <  1:3819513/140‑1 (MQ=255)

                     cccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                  <  1:3735853/140‑1 (MQ=255)

                     cccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                  <  1:1507854/140‑1 (MQ=255)

                     cccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                  <  1:618251/140‑1 (MQ=255)

                     cccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                  <  1:2983742/140‑1 (MQ=255)

                     cccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCACAAATCTTTAACGGCcaca                                                                                                                  <  2:1017748/140‑1 (MQ=255)

                      ccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACAt                                                                                                                 <  1:72191/140‑1 (MQ=255)

                        aaCGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATgg                                                                                                               >  1:4460401/1‑140 (MQ=255)

                          cGCCATCCCCTGACCCAGTCCAGTATCACGACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTc                                                                                                             <  2:4848363/140‑1 (MQ=255)

                          cGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTc                                                                                                             <  1:2024982/140‑1 (MQ=255)

                           gCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTcc                                                                                                            <  2:4360335/140‑1 (MQ=255)

                              aTCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCata                                                                                                         <  1:2599721/140‑1 (MQ=255)

                               tCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcac                                                                                                                   >  2:1565212/1‑129 (MQ=255)

                               tCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcac                                                                                                                   <  1:1565212/129‑1 (MQ=255)

                               tCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCatat                                                                                                        >  1:4113169/1‑140 (MQ=255)

                               tCCCCTGACCAAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTACGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCatat                                                                                                        >  2:1655141/1‑140 (MQ=255)

                                ccccTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATatc                                                                                                       >  2:2511000/1‑140 (MQ=255)

                                    tGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGCGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATAtcatca                                                                                                   <  2:4397255/140‑1 (MQ=255)

                                          agtccagtATCACAACCATTGACGACCGCAGCTTTACCTTCGGGAGAAAATGCACTTAGAATCATTACGATGCCGTAGTCGTGTATGCCCACAACTAGCGCATTTCCTTAACGGCCACATTGTCCATACTGTCATAGAcc                                                                                             >  2:28773/1‑140 (MQ=255)

                                           gtccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCt                                                                                            >  1:2764053/1‑140 (MQ=255)

                                           gtccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCt                                                                                            >  1:1473512/1‑140 (MQ=255)

                                            tccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTCAACTCATAACAATACCTTATTCGTTTATTCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTg                                                                                           >  1:1181537/1‑140 (MQ=255)

                                            tccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTg                                                                                           >  1:3588016/1‑140 (MQ=255)

                                            tccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTg                                                                                           >  2:1462839/1‑140 (MQ=255)

                                            tccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCAGATCATCAAAGACCTg                                                                                           >  2:3361393/1‑140 (MQ=255)

                                                 tATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGtttt                                                                                      >  1:43502/1‑140 (MQ=255)

                                                      caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATTCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAATACCTGGTTTTAAcct                                                                                 >  2:1735830/1‑139 (MQ=255)

                                                      caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCg                                                                                 >  2:4987360/1‑140 (MQ=255)

                                                      caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCg                                                                                 >  1:1618190/1‑140 (MQ=255)

                                                      caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCg                                                                                 >  2:4607815/1‑140 (MQ=255)

                                                      caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCg                                                                                 >  1:58794/1‑140 (MQ=255)

                                                       aaCCAGTGACGACCGCCACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGa                                                                                <  1:2828934/140‑1 (MQ=255)

                                                         ccAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGAcc                                                                              <  1:2677447/140‑1 (MQ=255)

                                                           aGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCAt                                                                            >  2:447727/1‑140 (MQ=255)

                                                            gTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATg                                                                           >  1:2307381/1‑140 (MQ=255)

                                                              gacgacCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                                                         <  2:2339827/140‑1 (MQ=255)

                                                              gacgacCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                                                         <  1:2120222/140‑1 (MQ=255)

                                                              gacgacCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                                                         <  1:922478/140‑1 (MQ=255)

                                                              gacgacCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                                                         <  1:4776069/140‑1 (MQ=255)

                                                              gacgacCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                                                         <  1:701602/140‑1 (MQ=255)

                                                               acccccGCAACTTATCCCTCGATAGAAAATCCACTTACAATCATACCAATACCTTATTCGTTTATGCACACAACTACCGCAAATCTTTACCGACCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACAATGccc                                                                        <  2:1917042/136‑1 (MQ=255)

                                                                    cGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGAt                                                                   <  2:1473512/140‑1 (MQ=255)

                                                                    cGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAACGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGAt                                                                   >  1:3874666/1‑140 (MQ=255)

                                                                     gCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATa                                                                  <  2:5078935/140‑1 (MQ=255)

                                                                     gCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATa                                                                  <  1:2686545/140‑1 (MQ=255)

                                                                           ttACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGta                                                            <  1:354388/140‑1 (MQ=255)

                                                                                   gagagaAAATGCCCTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCttt                                                    <  2:3602142/140‑1 (MQ=255)

                                                                                        aaaaTGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTc                                               >  1:3975129/1‑140 (MQ=255)

                                                                                           aTGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCGAATCTTTAACGGCCACATGTTCCATCTCATCAAAGACCTGGTTTTCACCGACCCTGCCCCAGATAAAGGTATAAGCTTTGTTTCCgc                                            >  2:3656381/1‑139 (MQ=255)

                                                                                           aTGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGa                                            <  1:327196/140‑1 (MQ=255)

                                                                                             gCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                                          <  1:3122281/140‑1 (MQ=255)

                                                                                             gCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                                          <  1:655907/140‑1 (MQ=255)

                                                                                             gCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                                          <  1:2467298/140‑1 (MQ=255)

                                                                                             gCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                                          <  1:266829/140‑1 (MQ=255)

                                                                                             gCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCACAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                                          <  1:1068365/140‑1 (MQ=255)

                                                                                             acACTTAAAATCATAACAATACCTTATTCGTTTCTGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATACAGGTATAAGCTTTGGTTCCGaca                                          <  1:3147607/139‑1 (MQ=255)

                                                                                                  tAAAATCATAACAATACCGTAGTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGa                                     <  1:3656381/140‑1 (MQ=255)

                                                                                                         aTAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATc                              >  1:4419995/1‑140 (MQ=255)

                                                                                                           aaCAATACCTTATTCGTTTATGCGCACAACTAGCGCAAATCTTTAACGGCCACATGGTCGATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGa                            >  2:4029364/1‑140 (MQ=255)

                                                                                                           aaCAATACCTTATTCGTTTATGCCCACCACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACTATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGa                            <  2:2724288/140‑1 (MQ=255)

                                                                                                           aaCAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGa                            >  2:4256583/1‑140 (MQ=255)

                                                                                                           aaCAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGa                            <  1:4650449/140‑1 (MQ=255)

                                                                                                            acaacaCCTTGTTCGTTGATGCGCACAACTAGCGCAAATCTTGAACGGCCACATCGTACATATCATCAAAGAACTGGTTTTGACCGACCATGCCCCAGATAAAAGTATAAGGTTAAGTTCCGACACCGGAATGGTTCGAc                           <  2:2356138/140‑1 (MQ=255)

                                                                                                            aCAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGAc                           <  2:2011716/140‑1 (MQ=255)

                                                                                                             cAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGAcc                          <  2:3076165/140‑1 (MQ=255)

                                                                                                                tACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGc                       >  1:4724800/1‑140 (MQ=255)

                                                                                                                     tATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCggg                  >  2:185891/1‑140 (MQ=255)

                                                                                                                     tATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCggg                  >  1:1761939/1‑140 (MQ=255)

                                                                                                                     tATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCggg                  <  1:1524262/140‑1 (MQ=255)

                                                                                                                      aTTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCgggg                 <  1:841871/140‑1 (MQ=255)

                                                                                                                       ttCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATa                                                                  >  2:854488/1‑90 (MQ=255)

                                                                                                                       ttCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATa                                                                  <  1:854488/90‑1 (MQ=255)

                                                                                                                        tCGTTTATGCCCACAACTAGCGCAAAACTTAAACGGCCACATGGTCCATATGATCAAAGACCTGGTTTTCACAGACCATGCCCCAGATAAGGGTATAGGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGgag               >  1:3050492/1‑140 (MQ=255)

                                                                                                                          gTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGAt             <  2:2972884/140‑1 (MQ=255)

                                                                                                                           tttATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGTGAGATc            <  1:2183632/140‑1 (MQ=255)

                                                                                                                            ttATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTCTAAGCTGTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGAGCa           >  2:2143462/1‑140 (MQ=255)

                                                                                                                            ttATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCa           >  2:3131821/1‑140 (MQ=255)

                                                                                                                            ttATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCa           >  1:4523921/1‑140 (MQ=255)

                                                                                                                             tATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCAc          >  2:2630419/1‑140 (MQ=255)

                                                                                                                                gCCCACAACTCGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGc       <  2:3876873/140‑1 (MQ=255)

                                                                                                                                gCCCACAACTAGCGCAAATCTTTAACGGCCACATTGTCCATATCATCAGAGCCCTGGTTGTCACCGACCATGCCCCGGATAAGGGGGTAAGGTTTGGTTCCGGCACCGGAATGGATGGACCAGCTCGTGGAGATCACCGc       >  2:1314840/1‑140 (MQ=255)

                                                                                                                                     caACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGCt  <  2:3553649/140‑1 (MQ=255)

TCACAGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGCT  >  NZ_CP025268/2985457‑2985729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`