BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,070,477	T→C	212 (TAA→TAG)	argO ←	arginine exporter ArgO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,070,477	0	T	C	99.5%	1247.5 / NA	373	212 (TAA→TAG)	argO	arginine exporter ArgO
Reads supporting (aligned to +/- strand): ref base T (1/0); major base C (147/223); minor base A (0/2); total (148/225)
Fisher's exact test for biased strand distribution p-value = 5.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 373 total aligned reads displayed.

CTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACGCGGTGCCAGCCAGGCTG  >  NZ_CP025268/3070341‑3070613

cTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTaa                                                                                                                                       <  2:4613612/140‑1 (MQ=255)

cTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTGTCCATCAGATAGCGCCTaa                                                                                                                                       <  2:2974566/140‑1 (MQ=255)

cTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGGTAGTCTCTCCGTGTTAACTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTaa                                                                                                                                       >  1:1024622/1‑140 (MQ=255)

 tAATACCCATTAATGCCGCCAGGGCGATAACTTTGACCTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAAc                                                                                                                                      <  1:2744268/140‑1 (MQ=255)

 cactACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTCGAGGCAAGCTTAGCGCCTCTGTTTTATTCTTCCATCAGATAGCGCCTAAc                                                                                                                                      <  2:96454/137‑1 (MQ=255)

     aCCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGaa                                                                                                                                  <  1:1802607/140‑1 (MQ=255)

       ccATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACa                                                                                                                                <  2:1285265/140‑1 (MQ=255)

         aTTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAg                                                                                                                              >  1:4208816/1‑140 (MQ=255)

         aTTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAg                                                                                                                              >  1:1719524/1‑140 (MQ=255)

         aTTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAg                                                                                                                              >  2:3695616/1‑140 (MQ=255)

         aTTAATGCCGCCAGGGCGATAACTTTGAACTTCACGCTAGCCTCTCCGTGTTAATTAACTTGCCCAAAATTCAGAACCGCTGGAGGCAAGCTTATCGCCACTGTATTATTTTTCCATCAGATAGCGCCCAACTGAACAAg                                                                                                                              >  2:1196745/1‑140 (MQ=255)

            aaTGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCt                                                                                                                           <  1:4113496/140‑1 (MQ=255)

                 cgccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGc                                                                                                                      <  1:2769946/140‑1 (MQ=255)

                 cgccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGc                                                                                                                      <  1:4010296/140‑1 (MQ=255)

                  gccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCa                                                                                                                     >  2:542391/1‑140 (MQ=255)

                  gccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCa                                                                                                                     <  2:2154849/140‑1 (MQ=255)

                   ccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCAt                                                                                                                    >  2:2662190/1‑140 (MQ=255)

                   ccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCAt                                                                                                                    <  1:191928/140‑1 (MQ=255)

                   ccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCAt                                                                                                                    >  1:2241601/1‑140 (MQ=255)

                   ccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGCGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCAt                                                                                                                    >  2:1895591/1‑140 (MQ=255)

                      gggCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGGTAGCGCCTAACTGAACAAGGCTTGTGCATGAg                                                                                                                 <  2:4330910/140‑1 (MQ=255)

                           aTAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATa                                                                                                            >  1:885570/1‑140 (MQ=255)

                            tAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATAc                                                                                                           >  2:1536009/1‑140 (MQ=255)

                            tAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATAc                                                                                                           <  1:1157100/140‑1 (MQ=255)

                            tAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATAc                                                                                                           <  2:2924461/140‑1 (MQ=255)

                                   gAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGtctct                                                                                                    <  1:5098968/140‑1 (MQ=255)

                                      cTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGc                                                                                                 >  1:2353802/1‑140 (MQ=255)

                                       ttCACGTTAGTCTCTCCGTGTTTATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGcc                                                                                                <  1:4275325/140‑1 (MQ=255)

                                         cACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAg                                                                                              >  1:4679276/1‑140 (MQ=255)

                                              tAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGCCCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCa                                                                                         <  1:4392807/140‑1 (MQ=255)

                                              tAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCa                                                                                         <  2:3774411/140‑1 (MQ=255)

                                              tAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCa                                                                                         <  2:1878006/140‑1 (MQ=255)

                                               aGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCaa                                                                                        <  1:4456068/140‑1 (MQ=255)

                                                 actctcCGTGTTAATTGACTTGCCCAACATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGCTAGCGCCTAACGGAACAAGGCTTGTGCAGGAGCAATACCGTCTCTCGCCCGCTGCAAgg                                                                                      >  2:2380519/2‑140 (MQ=255)

                                                   tctcCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCGAGCTTAGCGCCTCCGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCa                                                                                    >  2:716479/1‑140 (MQ=255)

                                                   tctcCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCa                                                                                    <  2:3138067/140‑1 (MQ=255)

                                                     tcCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAAt                                                                                  >  1:790743/1‑140 (MQ=255)

                                                      ccGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGCGGCAAGCTTAGCGCCTCTGTTTTATTTTGCCATCAGATAGCGCCGAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATa                                                                                 <  2:2329454/140‑1 (MQ=255)

                                                      ccGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATa                                                                                 >  1:356412/1‑140 (MQ=255)

                                                      ccGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATa                                                                                 <  2:1099142/140‑1 (MQ=255)

                                                        gtgtTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATaaa                                                                               <  1:3761340/140‑1 (MQ=255)

                                                        gtgtTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATaaa                                                                               <  1:3605631/140‑1 (MQ=255)

                                                        gtgtTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATaaa                                                                               <  1:2523620/140‑1 (MQ=255)

                                                          gtTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAAcc                                                                             >  2:2003669/1‑140 (MQ=255)

                                                           ttAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACca                                                                            >  2:1426676/1‑140 (MQ=255)

                                                           ttAATTGACTTGCCCAAAATGCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACca                                                                            >  2:5052323/1‑140 (MQ=255)

                                                             aaTTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACcaca                                                                          <  2:2711065/140‑1 (MQ=255)

                                                               ttGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATa                                                                        >  1:2072591/1‑140 (MQ=255)

                                                                   cTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTACCTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAacac                                                                    <  2:2606559/140‑1 (MQ=255)

                                                                   cTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAaccc                                                                    <  1:2423477/140‑3 (MQ=255)

                                                                   cTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAacac                                                                    <  1:1463308/140‑1 (MQ=255)

                                                                   cTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAacac                                                                    <  1:2006866/140‑1 (MQ=255)

                                                                   cTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAacac                                                                    >  1:3038773/1‑140 (MQ=255)

                                                                   cTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAacac                                                                    <  2:409303/140‑1 (MQ=255)

                                                                   cTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAAGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAacac                                                                    <  2:3644427/140‑1 (MQ=255)

                                                                       cccAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATcc                                                                <  2:5091141/140‑1 (MQ=255)

                                                                       cccAAAATTCAGGAACTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATCAGCAATCCCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACCCATcc                                                                <  2:33732/140‑1 (MQ=255)

                                                                         cAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCca                                                              <  2:1738096/140‑1 (MQ=255)

                                                                             aTTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAAc                                                          <  2:349653/140‑1 (MQ=255)

                                                                             aTTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAAc                                                          <  1:2963324/140‑1 (MQ=255)

                                                                             aTTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAAc                                                          <  1:3243219/140‑1 (MQ=255)

                                                                             aTTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAAc                                                          <  2:4815514/140‑1 (MQ=255)

                                                                             aTTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAAc                                                          <  1:988471/140‑1 (MQ=255)

                                                                             aTTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAAc                                                          <  1:1786002/140‑1 (MQ=255)

                                                                                  ttaCCTCTGGAGGCATGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATATCGCCTAACGGAACAAGGCTTGTGCATCAGCAACACCGTCCCTCGCCAGCTGCAAGGCAATCAACCACATAATACATCCCACAACCAGAt                                                     <  2:2512372/138‑1 (MQ=255)

                                                                                  ggACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGAt                                                     <  1:1370179/140‑1 (MQ=255)

                                                                                  ggACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGAt                                                     <  2:2241601/140‑1 (MQ=255)

                                                                                  ggACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGAt                                                     <  2:4926528/140‑1 (MQ=255)

                                                                                  ggACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGAt                                                     <  1:3330498/140‑1 (MQ=255)

                                                                                  ggACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGAt                                                     <  1:64788/140‑1 (MQ=255)

                                                                                        ctGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATaa                                               >  1:1094325/1‑140 (MQ=255)

                                                                                             ggCAAGCTTAGCTCCTCTGTTTTATTTTTCCATCCGAGAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGCCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATgcgc                                          <  2:2087962/140‑1 (MQ=255)

                                                                                             ggCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATgcgc                                          <  2:2810111/140‑1 (MQ=255)

                                                                                             ggCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATgcgc                                          <  2:4476422/140‑1 (MQ=255)

                                                                                             ggCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATgcgc                                          <  2:4066822/140‑1 (MQ=255)

                                                                                               cAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCtg                                        <  2:3174193/140‑1 (MQ=255)

                                                                                               cAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCtg                                        <  2:1706371/140‑1 (MQ=255)

                                                                                                aaGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCtgt                                       >  1:4948667/1‑140 (MQ=255)

                                                                                                aaGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCtgt                                       >  1:3704279/1‑140 (MQ=255)

                                                                                                aaGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCtgt                                       >  1:3889093/1‑140 (MQ=255)

                                                                                                 accTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGCCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCtgtg                                      <  2:4146399/138‑1 (MQ=255)

                                                                                                  gCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGc                                     >  1:3082816/1‑140 (MQ=255)

                                                                                                            tctGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTg                                                                                           <  1:4669198/76‑1 (MQ=255)

                                                                                                            tctGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTg                                                                                           >  2:4667663/1‑76 (MQ=255)

                                                                                                             ctGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAACCCCCATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTgc                          >  1:3288904/1‑140 (MQ=255)

                                                                                                             ctGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCGTTTGCCGTgc                          >  2:608218/1‑140 (MQ=255)

                                                                                                              tGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTgcg                         <  2:4679276/140‑1 (MQ=255)

                                                                                                                    aTTTTTCCATCAGGAAGCTCCTCACTGAACAAGGCTTGTGCAAGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGACAATGCGCTGTGCTTTTGCCGTGCGCAGAcg                   <  2:4790585/140‑1 (MQ=255)

                                                                                                                    aTTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGAcg                   <  1:4873793/140‑1 (MQ=255)

                                                                                                                    aTTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGAcg                   <  2:812291/140‑1 (MQ=255)

                                                                                                                        ttCCATCATATAGCGCATGCCTGAAAAAGGCTTGTGCATGCGCAATACCGCCTCTCGCCCCCTGCGAGGCAATAAACCACATATCACATCCGACAACCGGATTGATACTGCGCTGTGCTTTTGCCGTGGGCAGACGCGGt               <  2:3734993/140‑1 (MQ=255)

                                                                                                                        ttCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACGCGGt               <  2:3940912/140‑1 (MQ=255)

                                                                                                                        ttCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACGCGGt               >  1:1222997/1‑140 (MQ=255)

                                                                                                                        ttCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACGCGGt               <  1:736520/140‑1 (MQ=255)

                                                                                                                        ttCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACGCGGt               <  2:3889093/140‑1 (MQ=255)

                                                                                                                               aGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACGCGGTgccagcc        <  2:3082816/140‑1 (MQ=255)

                                                                                                                                gATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACGCGGTgccagcca       >  1:1251825/1‑140 (MQ=255)

                                                                                                                                   aGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACGCGGTGCCAGCCAGGc    <  1:269314/140‑1 (MQ=255)

                                                                                                                                     cgcCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACGCGGTGCCAGCCAGGCTg  <  1:3376273/140‑1 (MQ=255)

                                                                                                                                     cgcCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATGGATAATGCGCTGTGCTTTTGCCATGCGCAGACGCGGTGCCAGCCAGGCTg  >  2:4393808/1‑140 (MQ=255)

CTAATACCCATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGCGCAGACGCGGTGCCAGCCAGGCTG  >  NZ_CP025268/3070341‑3070613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`