BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,174,183	T→C	111 (TAA→TAG)	ygiZ ←	DUF2645 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,174,183	0	T	C	99.4%	523.5 / NA	156	111 (TAA→TAG)	ygiZ	DUF2645 family protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (45/110); minor base A (0/1); total (45/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 156 total aligned reads displayed.

TGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAGT  >  NZ_CP025268/3174050‑3174315

tGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGATTGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAg                                                                                                                                >  2:2826868/1‑140 (MQ=255)

tGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAg                                                                                                                                >  2:886397/1‑140 (MQ=255)

tGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAg                                                                                                                                >  2:2596456/1‑140 (MQ=255)

tGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAg                                                                                                                                >  1:2678852/1‑140 (MQ=255)

 gTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGc                                                                                                                               <  1:144351/140‑1 (MQ=255)

 gTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGc                                                                                                                               >  2:2872815/1‑140 (MQ=255)

 gTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGc                                                                                                                               <  1:4083392/140‑1 (MQ=255)

 gTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAAGACTCTCATAGATTTTACTAATAGc                                                                                                                               <  2:4586481/140‑1 (MQ=255)

  tCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                              <  1:1196186/140‑1 (MQ=255)

  tCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                              <  2:166399/140‑1 (MQ=255)

  tCCAGCGAATCGCCAAATTGCAAGGCAAAAATGTTAAATATGGGAATGCAGAACAAGGTGGATTTGACGCGAAGGTAACCTGGTAACTTCTCTGTCTATGAGAGCCGGTAAAACGAATCTCATAGATTTTACTAATAGCa                                                                                                                              <  2:410425/140‑1 (MQ=255)

   ccAGCGAATCGCCAACTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTACCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCaa                                                                                                                             <  2:4372447/140‑1 (MQ=255)

   ccAGCGAATCGCCAAATTTCAGCGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGCTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTGACTAATAGCaa                                                                                                                             <  2:3640571/140‑1 (MQ=255)

   ccAGCGAATCGCCAAATTGCTTGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCaa                                                                                                                             <  1:1728892/140‑1 (MQ=255)

   ccAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCaa                                                                                                                             <  1:3536269/140‑1 (MQ=255)

   ccAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCaa                                                                                                                             <  1:124920/140‑1 (MQ=255)

       cGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAata                                                                                                                         >  2:4546004/1‑140 (MQ=255)

            cGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAAc                                                                                                                    >  1:1996157/1‑140 (MQ=255)

             gCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAAcc                                                                                                                   <  1:185963/140‑1 (MQ=255)

               aaaaTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGt                                                                                                                 <  2:4141328/139‑1 (MQ=255)

                        tggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaaaa                                                                                                        <  2:4908412/140‑1 (MQ=255)

                                    tGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCaccaacca                                                                                            >  2:2474889/1‑140 (MQ=255)

                                     gAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGAGTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac                                                                                           >  2:4613740/1‑140 (MQ=255)

                                     gAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac                                                                                           >  1:4766655/1‑140 (MQ=255)

                                         ttGGTAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                       <  2:2206868/140‑1 (MQ=255)

                                         ttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                       <  2:2697152/140‑1 (MQ=255)

                                         ttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                       <  2:2698000/140‑1 (MQ=255)

                                         ttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                       <  2:280252/140‑1 (MQ=255)

                                         ttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                       <  1:209335/140‑1 (MQ=255)

                                         ttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                       <  2:128869/140‑1 (MQ=255)

                                         ttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                       <  2:128783/140‑1 (MQ=255)

                                         ttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                       <  1:4385100/140‑1 (MQ=255)

                                         ttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                       <  1:57734/140‑1 (MQ=255)

                                         ttGGGAATGCGCCACTAGGTTGACTTGAGGCGCCGGTATGCCGGTAGACCCCCTGTCTATGAGTGCCGCTAAAACGACTCTCATAGACTTTCCGAATAGCAAAAGATAAACCGCCCCCAAAAAAGCCACCAACCACAAAc                                                                                       <  2:1881976/140‑1 (MQ=255)

                                             gAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGa                                                                                   <  1:2596456/140‑1 (MQ=255)

                                               aTGCGGAACAAGGTTGATTTGAGTCAAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGATAAAACGCGTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACa                                                                                 <  2:4517502/140‑1 (MQ=255)

                                               aTGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGATGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAAGAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACa                                                                                 <  1:1052191/140‑1 (MQ=255)

                                                tGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCGATGAGAGCCGTTAAAACGACTCTCATAGATTTGACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACaa                                                                                >  2:2547066/1‑140 (MQ=255)

                                                        aaGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                        <  2:2569753/140‑1 (MQ=255)

                                                        aaGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                        <  1:3935588/140‑1 (MQ=255)

                                                        aaGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                        <  2:1840118/140‑1 (MQ=255)

                                                        aaGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                        <  2:4620138/140‑1 (MQ=255)

                                                        aaGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGGCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                        <  1:1143954/140‑1 (MQ=255)

                                                        aaGGTGGATCTGAGGCGAAGGTAAGCTGCTATCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTCCTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                        <  2:2296445/140‑1 (MQ=255)

                                                         tggTGGATTCGAGGCCAAGGTAAGCTAGTAACTTCTCTGTTTATGAGAGCCGTAAAAACAACTCTCATAGATTTTACCAATAGCAAAATATAAACCGCCCCCACACAATCCACCAACCACAAACCAGACAAATACcgagc                                                                       <  2:3977981/139‑1 (MQ=255)

                                                           gTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcga                                                                     >  1:2095883/1‑140 (MQ=255)

                                                            tGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTCCTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcgag                                                                    <  2:1692585/140‑1 (MQ=255)

                                                            tGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcgag                                                                    <  1:3848229/140‑1 (MQ=255)

                                                            tGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcgag                                                                    <  1:1748923/140‑1 (MQ=255)

                                                            tGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcgag                                                                    >  1:3908900/1‑140 (MQ=255)

                                                            tGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcgag                                                                    <  2:640242/140‑1 (MQ=255)

                                                              gATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa                                                                  <  2:628442/140‑1 (MQ=255)

                                                              gATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa                                                                  <  1:4441837/140‑1 (MQ=255)

                                                              gATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa                                                                  <  1:2256517/140‑1 (MQ=255)

                                                              gATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa                                                                  <  1:3856393/140‑1 (MQ=255)

                                                              gATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa                                                                  <  2:3263118/140‑1 (MQ=255)

                                                              gATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTATCATAGAGTTTACTAATAGCAAAATATAAACCGTCCACAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa                                                                  <  2:3923091/140‑1 (MQ=255)

                                                              caTTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa                                                                  <  1:4509134/139‑1 (MQ=255)

                                                              aaTTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa                                                                  <  1:4508989/139‑1 (MQ=255)

                                                               aTTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAAt                                                                 >  1:2615723/1‑140 (MQ=255)

                                                                 ttGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATAc                                                               <  1:3325456/140‑1 (MQ=255)

                                                                    aGGCGAAGGTAAGTTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACata                                                            <  1:4497194/140‑1 (MQ=255)

                                                                    aGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACata                                                            <  1:4169654/140‑1 (MQ=255)

                                                                          aGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCa                                                      <  1:4693401/140‑1 (MQ=255)

                                                                           ggTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTCATAGCAAAATATAAACCGTCCCCAACAAAGCCACCAACCACATACCAGACAAGTCCCGAGCGAGAATACATATACCCaa                                                     <  2:522117/140‑1 (MQ=255)

                                                                               aGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACt                                                 >  1:2392133/1‑140 (MQ=255)

                                                                                  tGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGa                                              <  1:3451290/140‑1 (MQ=255)

                                                                                          tctcTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTcc                                      <  1:741415/140‑1 (MQ=255)

                                                                                          tctcTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTcc                                      <  2:4918999/140‑1 (MQ=255)

                                                                                          tctcTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTcc                                      <  1:4777896/140‑1 (MQ=255)

                                                                                              tGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCa                                  >  1:3774960/1‑140 (MQ=255)

                                                                                               ggcTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCGCAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACGCGAACATTTCCCGCAt                                 >  1:4084972/3‑140 (MQ=255)

                                                                                                   aTGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAag                             >  2:5043068/1‑140 (MQ=255)

                                                                                                   aTGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAag                             >  2:1639600/1‑140 (MQ=255)

                                                                                                      agagCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGACAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGt                          <  2:1628436/140‑1 (MQ=255)

                                                                                                          ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACt                                                 >  1:1182168/1‑113 (MQ=255)

                                                                                                          ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACt                                                 <  2:1182168/113‑1 (MQ=255)

                                                                                                          ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTc                      >  2:374667/1‑140 (MQ=255)

                                                                                                          ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTc                      >  1:4722539/1‑140 (MQ=255)

                                                                                                          ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTc                      >  2:857765/1‑140 (MQ=255)

                                                                                                          ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTc                      >  1:1034813/1‑140 (MQ=255)

                                                                                                           cGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCt                     <  2:1431696/140‑1 (MQ=255)

                                                                                                           cGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCAAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCt                     <  1:1689518/140‑1 (MQ=255)

                                                                                                            gTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCtt                    <  2:532513/140‑1 (MQ=255)

                                                                                                            gTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCtt                    <  1:2760352/140‑1 (MQ=255)

                                                                                                            gTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCtt                    <  1:4424614/140‑1 (MQ=255)

                                                                                                              tAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaa                  >  2:2487594/1‑140 (MQ=255)

                                                                                                                 accGACTCTCATAGATTTTAATCCTCGCACAATATATACCGTCCCCCAAAAAGCCCCCAACCCCAAACCACACATATACCGAGCGAGAATACATTTACCATACTCTCGAACATTTCCCGCATAAAAAGTTTTCTtacgct               <  2:2783544/138‑5 (MQ=255)

                                                                                                                 aaCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGTGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagat               <  2:4181803/140‑1 (MQ=255)

                                                                                                                 aaCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagat               <  1:5014389/140‑1 (MQ=255)

                                                                                                                 aaCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagat               <  1:219554/140‑1 (MQ=255)

                                                                                                                 aaCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagat               <  1:2970370/140‑1 (MQ=255)

                                                                                                                 aaCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagat               <  2:1661176/140‑1 (MQ=255)

                                                                                                                  aCGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagata              <  1:1497232/140‑1 (MQ=255)

                                                                                                                   cGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagataa             <  1:804933/140‑1 (MQ=255)

                                                                                                                   cGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCAACAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagatta             <  2:3460067/140‑3 (MQ=255)

                                                                                                                     aCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTtaagataaga           >  1:4607599/1‑140 (MQ=255)

                                                                                                                       tctcATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGaat         >  2:1464251/1‑140 (MQ=255)

                                                                                                                           aTAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAataata     >  2:915370/1‑140 (MQ=255)

                                                                                                                            tAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAAtaataa    <  2:3810845/140‑1 (MQ=255)

TGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAGT  >  NZ_CP025268/3174050‑3174315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`