BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,232,860	T→C	N170N (AAT→AAC)	fadH →	NADPH‑dependent 2,4‑dienoyl‑CoA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,232,860	0	T	C	100.0%	1126.9 / NA	330	N170N (AAT→AAC)	fadH	NADPH‑dependent 2,4‑dienoyl‑CoA reductase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (175/155); total (175/155)

Only 100 of 330 total aligned reads displayed.

AACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGA  >  NZ_CP025268/3232723‑3232995

 aCCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGaa                                                                                                                                      >  2:1950981/1‑140 (MQ=255)

   cGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAAtt                                                                                                                                    >  2:4172532/1‑140 (MQ=255)

     tttCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTc                                                                                                                                  >  2:25468/1‑140 (MQ=255)

       tCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGTCAGGATACGACGGTGTAGAGGTCATGGGTTCCGAAGGGTATTAGATCAACGAATTTCTg                                                                                                                                >  1:2851409/1‑140 (MQ=255)

        cGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACCATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGa                                                                                                                               <  1:3145669/140‑1 (MQ=255)

            ccccATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCt                                                                                                                           <  1:1480456/140‑1 (MQ=255)

             cccATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTg                                                                                                                          >  1:5104682/1‑140 (MQ=255)

                 tGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCa                                                                                                                      >  1:4685314/1‑140 (MQ=255)

                   aGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCAcc                                                                                                                    <  2:1719634/140‑1 (MQ=255)

                      tcaGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGTGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCTAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAAt                                                                                                                 <  2:4263684/138‑1 (MQ=255)

                      tAAGGCATGCAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAAt                                                                                                                 <  2:351821/140‑1 (MQ=255)

                      tAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAAt                                                                                                                 <  2:3301112/140‑1 (MQ=255)

                      tAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAAt                                                                                                                 <  1:480970/140‑1 (MQ=255)

                      tAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAAt                                                                                                                 <  1:4859177/140‑1 (MQ=255)

                      tAAGCCATGAAGAGAGCCTGCAACTGATCGACAAGTGCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAAt                                                                                                                 <  2:2329051/140‑1 (MQ=255)

                        aGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCa                                                                                                               <  1:1717797/140‑1 (MQ=255)

                        aGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCa                                                                                                               <  1:2074639/140‑1 (MQ=255)

                        aGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCa                                                                                                               <  1:2289336/140‑1 (MQ=255)

                        aGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCa                                                                                                               <  2:4500591/140‑1 (MQ=255)

                        aGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCa                                                                                                               <  2:549559/140‑1 (MQ=255)

                        aGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTGCTGACGCTGCGCACCAATCa                                                                                                               >  1:3760032/1‑140 (MQ=255)

                           cATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCg                                                                                                            <  2:1588941/140‑1 (MQ=255)

                             tGAAGAGATCCTGCAACTGATCGGCAATTTGGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGCGTAGAGGTGATGGGTTCCGAAGGGCATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTa                                                                                                          >  1:3068967/1‑140 (MQ=255)

                             tGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGATGTGATGGGTTGCGAAGGGTATTAGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTa                                                                                                          >  2:1201944/1‑140 (MQ=255)

                             tGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTa                                                                                                          <  1:1288804/140‑1 (MQ=255)

                             tGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTa                                                                                                          <  2:666221/140‑1 (MQ=255)

                             tGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTa                                                                                                          >  1:1418255/1‑140 (MQ=255)

                             tGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTa                                                                                                          >  1:2492517/1‑140 (MQ=255)

                                    aTCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCa                                                                                                   >  1:901121/1‑140 (MQ=255)

                                    aTCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCa                                                                                                   >  1:1163843/1‑140 (MQ=255)

                                    aTCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCa                                                                                                   >  1:2404471/1‑140 (MQ=255)

                                       cTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTg                                                                                                >  2:2910595/1‑140 (MQ=255)

                                         gCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTAGTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTggg                                                                                              >  2:4391571/1‑140 (MQ=255)

                                         gCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGAATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTggg                                                                                              >  2:1022430/1‑140 (MQ=255)

                                            aCTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGACGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGggcg                                                                                           <  2:4909476/140‑1 (MQ=255)

                                                aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGa                                                                                       >  2:2575683/1‑140 (MQ=255)

                                                aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGa                                                                                       >  2:4112582/1‑140 (MQ=255)

                                                aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGa                                                                                       >  1:771601/1‑140 (MQ=255)

                                                aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGa                                                                                       >  2:4656365/1‑140 (MQ=255)

                                                aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGAGGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGa                                                                                       >  2:1594824/1‑140 (MQ=255)

                                                 tCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGAt                                                                                      >  1:380050/1‑140 (MQ=255)

                                                  cGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGAtt                                                                                     >  2:4585150/1‑140 (MQ=255)

                                                     cAATTTCGCCAGATACGCGCAACTGGCGCGGGAAGAAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGACCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTAcc                                                                                  <  2:1402299/140‑1 (MQ=255)

                                                          tCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAAc                                                                             <  1:3580069/140‑1 (MQ=255)

                                                                  tGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCg                                                                     <  1:3456652/140‑1 (MQ=255)

                                                                    cgcgcAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGAt                                                                   <  2:2023834/140‑1 (MQ=255)

                                                                     gcgcAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGAtt                                                                  <  1:5032634/140‑1 (MQ=255)

                                                                        cAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGc                                                               <  1:25468/140‑1 (MQ=255)

                                                                          aCTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCg                                                             <  1:1536099/140‑1 (MQ=255)

                                                                          aCTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCg                                                             <  2:2711930/140‑1 (MQ=255)

                                                                          aCTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCg                                                             <  2:1659926/140‑1 (MQ=255)

                                                                          aCTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCg                                                             <  2:4966813/140‑1 (MQ=255)

                                                                          aCTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCg                                                             <  1:4459389/140‑1 (MQ=255)

                                                                          aCTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCg                                                             <  1:4172532/140‑1 (MQ=255)

                                                                          aCTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCg                                                             <  1:796402/140‑1 (MQ=255)

                                                                          aCTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCg                                                             <  2:5083162/140‑1 (MQ=255)

                                                                          aCTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGGTCCGAAGGGTATTTGAGCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCg                                                             <  1:1839182/140‑1 (MQ=255)

                                                                                     aGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAgtg                                                  <  2:3396919/140‑1 (MQ=255)

                                                                                        cAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAgtgcgt                                               >  1:3877816/1‑140 (MQ=255)

                                                                                         aGGGTACGACGGTGTAGAGGTGGTGGGTTCCGAAGGGTATTAGATCAACGCATGTCTGTCGCTGCGCACCATTCGGCGTAGTGACCAGTGGGGCGGCGATTGCCGCGGCCGGATGCGATTTGCCGTAGAAGTAgtgcgtg                                              <  2:3774750/140‑1 (MQ=255)

                                                                                         aGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAgtgcgtg                                              <  2:113827/140‑1 (MQ=255)

                                                                                         aGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAgtgcgtg                                              <  2:1985683/140‑1 (MQ=255)

                                                                                           gATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGtgcgtgcg                                            <  1:1223009/140‑1 (MQ=255)

                                                                                                    gtgtAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCTATTACCGCAACCGGATGCGATTTGCCGCAGACGTAGTGCGTGCGGTGCGCGaa                                   <  2:2842619/140‑1 (MQ=255)

                                                                                                     tgtAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAAc                                  >  2:3174304/1‑140 (MQ=255)

                                                                                                     tgtAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAAc                                  >  1:2744352/1‑140 (MQ=255)

                                                                                                     tgtAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTACGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAAc                                  >  2:2700697/1‑140 (MQ=255)

                                                                                                       tAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAAcgc                                >  1:1079357/1‑140 (MQ=255)

                                                                                                       tAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAAcgc                                >  2:1487092/1‑140 (MQ=255)

                                                                                                            gTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCATAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGAGGTGCGCGAACGCGTCgg                           >  2:3652026/1‑140 (MQ=255)

                                                                                                              gATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGACGATTACCCAAACCGGATGCGATTTGCCGTATAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCa                         >  2:300703/1‑140 (MQ=255)

                                                                                                               aTGGGTTCCGAAGGGTATTTGATCCACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGACGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                        >  2:4068339/1‑140 (MQ=255)

                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGGACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                        >  2:3093633/1‑140 (MQ=255)

                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACGAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                        >  2:4026323/1‑140 (MQ=255)

                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAAt                                                                                                                 >  2:1787901/1‑51 (MQ=255)

                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAAt                                                                                                                 <  1:1787901/51‑1 (MQ=255)

                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                        <  2:2190453/140‑1 (MQ=255)

                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                        >  2:897841/1‑140 (MQ=255)

                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                        >  2:763613/1‑140 (MQ=255)

                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                        >  2:4733180/1‑140 (MQ=255)

                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                        >  2:4605489/1‑140 (MQ=255)

                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                        >  2:1910578/1‑140 (MQ=255)

                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                        >  1:4594641/1‑140 (MQ=255)

                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                        >  2:2243295/1‑140 (MQ=255)

                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                        >  2:3834569/1‑140 (MQ=255)

                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                        >  1:2630968/1‑140 (MQ=255)

                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                        >  2:2619395/1‑140 (MQ=255)

                                                                                                                    ttCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACt                   <  1:684586/140‑1 (MQ=255)

                                                                                                                      ccGAAGGGTATTTGATCAACGAGTTTCTGACGCTGCGCACCAATCAGGGTGGGGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACGTc                 >  2:2670456/1‑140 (MQ=255)

                                                                                                                      ccGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTc                 >  2:2899694/1‑140 (MQ=255)

                                                                                                                       cGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCa                <  2:2864385/140‑1 (MQ=255)

                                                                                                                       cGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCa                <  2:3253685/140‑1 (MQ=255)

                                                                                                                       cGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCa                >  2:1843749/1‑140 (MQ=255)

                                                                                                                       cGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCa                <  1:1978454/140‑1 (MQ=255)

                                                                                                                         aaGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTCGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCatt              <  1:4377573/140‑1 (MQ=255)

                                                                                                                           gggTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCattat            <  2:4821451/140‑1 (MQ=255)

                                                                                                                              tATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTa                                                                                                          <  1:940672/43‑1 (MQ=255)

                                                                                                                              tATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACTACTGCATtattat         >  2:246193/1‑140 (MQ=255)

                                                                                                                              tATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATtattat         <  2:1571259/140‑1 (MQ=255)

                                                                                                                                tttGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCt       >  1:3372969/1‑140 (MQ=255)

AACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGA  >  NZ_CP025268/3232723‑3232995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`